期刊
VIROLOGY
卷 522, 期 -, 页码 281-291出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2018.07.001
关键词
Plant virus; Segmented single-stranded DNA genome; Transmission helper factor; Reassortment; Faba bean; Aphis craccivora; Acyrthosiphon pisum
类别
资金
- ERA-NET Plant Genomics RCA GENOMICS [040B]
- Trilateral Cooperation GABI-GENOPLANTE-MEC
- Agence Nationale de la Recherche (ANR)
- CNRS
- Grenoble Instruct Center (CNRS-CEA-UJF-EMBL) [ISBG: UMS 3518]
- FRISBI [ANR-10-INSB-05-02]
- GRAL within the Grenoble Partnership for Structural Biology (PSB) [ANR-10-LABX-49-01]
Nanoviruses possess a multipartite single-stranded DNA genome and are naturally transmitted to plants by various aphid species in a circulative non-propagative manner. Using the cloned genomic DNAs of faba bean necrotic stunt virus (FBNSV) for reconstituting nanovirus infections we analyzed the necessity of different virus components for infection and transmission by aphids. We found that in the absence of DNA-U1 and DNA-U2 symptom severity decreased, and in the absence of DNA-U1 the transmission efficiency decreased. Most significantly, we demonstrated that the protein encoded by DNA-N (NSP) is mandatory for aphid transmission. Moreover, we showed that the NSP of FBNSV could substitute for that of a distantly related nanovirus, pea necrotic yellow dwarf virus. Altering the FBNSV NSP by adding 13 amino acids to its carboxy-terminus resulted in an infectious but non-transmissible virus. We demonstrate that the NSP acts as a nanovirus transmission factor, the existence of which had been hypothesized earlier.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据