Article
Plant Sciences
Yuanyuan Jiang, Jiangrong Peng, Yunpeng Cao, Zhiqiang Han, Ling Zhang, Wenbing Su, Shunquan Lin, Yuan Yuan, Bin Wang, Xianghui Yang, Zhike Zhang
Summary: The study introduced a new method for observing the cell structure of Nicotiana benthamiana, significantly improving imaging by fluorescence microscopy and allowing clear visualization of cellular structures and protein interactions under high-power lens. This method provides insights and references for the study of cell structures and functions in other plants, demonstrating its applicability in live cell visualization through confocal scanning laser microscopy.
PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
(2021)
Article
Chemistry, Analytical
Ui Jin Lee, Yunkwang Oh, Oh Seok Kwon, Yong-Beom Shin, Moonil Kim
Summary: We developed a highly sensitive and specific bimolecular fluorescence complementation (BiFC)-based influenza A virus (IAV)-sensing system. The system utilizes a galactose/glucose-binding protein (GGBP) with a N-terminal large domain (YN1-172) and a C-terminal small domain (YC173-239) made up of enhanced yellow fluorescence protein (eYFP). The system demonstrated ultra-high sensitivity and selective responses for IAV detection, making it a simple and effective platform for IAV detection without the need for virus culture or RNA extraction processes.
Article
Biochemical Research Methods
Florent Velay, Melanie Soula, Marwa Mehrez, Clement Belbachir, Stefano D'Alessandro, Christophe Laloi, Patrice Crete, Ben Field
Summary: This study describes the development of a flexible modular cloning-based toolkit for bimolecular fluorescence complementation (BiFC) and proximity labelling in the chloroplast and other cellular compartments. The toolkit simplifies the construction of chloroplast fusion proteins, enables robust ratiometric quantification, and provides model positive and negative controls. The study highlights potential pitfalls in designing BiFC experiments, such as the choice of FP split, negative controls, cell type, and reference FP.
Article
Agriculture, Multidisciplinary
Yang Si-hua, Zhao Li-rong, Ding Sha, Tang Shi-qiao, Chen Chun, Zhang Huan-xin, Xu Chun-ling, Xie Hui
Summary: This study investigated the pathogenic process of Radopholus similis infection in tobacco plants and established a method for determining its pathogenicity. The nematode primarily parasitized the root cortex, leading to tissue decay. The study evaluated the pathogenicity of different populations of R. similis from various hosts, confirming the feasibility of the method.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2022)
Article
Biochemistry & Molecular Biology
Gul-i-Rayna Shahzad, Alessandro Passera, Giusva Maldera, Paola Casati, Iriti Marcello, Piero Attilio Bianco
Summary: Our study demonstrated that ePGPB and chitosan treatments showed significant biocontrol efficacy against CymRSV and CMV in Nicotiana benthamiana plants, leading to reduced symptom severity and enhanced plant height compared to virus-infected controls. However, these phenotypic traits were not correlated with relative virus quantification. Additionally, the upregulation of the EDS1 gene implied the involvement of a salicylic-acid-related defense pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
In Woong Park, In Sun Hwang, Eom-Ji Oh, Choon-Tak Kwon, Chang-Sik Oh
Summary: Clavibacter michiganensis causes bacterial canker and wilting in host plants. Nicotiana benthamiana is a surrogate host for C. michiganensis and C. capsici. C. michiganensis induced blister-like lesions, canker, and wilting in N. benthamiana, and the celA and pat-1 genes were not necessary for symptom development.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biology
Soyeon Lee, Dongkeun Park, Chunghun Lim, Jae-Ick Kim, Kyung-Tai Min
Summary: Mitochondria play a crucial role in the distribution and localization of energy in neurites, with recent studies highlighting the importance of local mRNA translation in axons. The research demonstrates that brain-derived neurotrophic factor (BDNF) induces local translation of mitochondrial initiation factor 3 (mtIF3) mRNA in axonal growth cones, leading to promotion of mitochondrial translation and maintenance of axonal growth. These findings provide new insights into the adaptive control of mitochondrial physiology and axonal development through local mtIF3 translation.
Article
Plant Sciences
Long He, Peng Jin, Xuan Chen, Tian-Ye Zhang, Kai-Li Zhong, Peng Liu, Jian-Ping Chen, Jian Yang
Summary: The study identified changes in host proteins during CWMV infection, with a focus on disruptions in chloroplast functions and decreased ABA concentrations. The suppression of the ABA pathway during CWMV infection and the induction of host defenses against CWMV through ABA treatment were highlighted.
Article
Biochemical Research Methods
Liu Chen, Zheng Ni, Jionggang Hua, Weicheng Ye, Keshu Liu, Tao Yun, Yinchu Zhu, Cun Zhang
Summary: This study confirmed the direct interaction between the gC protein of duck enteritis virus and seven host proteins (GNG2, AR1H1, PPP2CA, UBE2I, MCM5, NUBP1, HN1), further validated through MbYTH and BiFC analyses. These findings shed light on how DEV manipulates cellular machinery, enhancing our understanding of DEV pathogenesis.
JOURNAL OF PROTEOMICS
(2021)
Article
Plant Sciences
Aditya Banerjee, Aryadeep Roychoudhury
Summary: OsFEX was induced early under fluoride stress but suppressed after prolonged stress treatment. Overexpression of OsFEX was essential for generating high fluoride tolerance, rather than its early induction.
PLANT CELL REPORTS
(2021)
Review
Biochemistry & Molecular Biology
Ragunathan Devendran, Manish Kumar, Dibyendu Ghosh, Sneha Yogindran, Mir Jishan Karim, Supriya Chakraborty
Summary: This review provides a comprehensive overview of various aspects of Capsicum-infecting begomoviruses, including their emergence, epidemiology, pathogenesis mechanism, and management protocols.
TRENDS IN MICROBIOLOGY
(2022)
Article
Neurosciences
Emily K. Don, Alina Maschirow, Rowan A. W. Radford, Natalie M. Scherer, Andres Vidal-Itriago, Alison Hogan, Cindy Maurel, Isabel Formella, Jack J. Stoddart, Thomas E. Hall, Albert Lee, Bingyang Shi, Nicholas J. Cole, Angela S. Laird, Andrew P. Badrock, Roger S. Chung, Marco Morsch
Summary: Amyotrophic lateral sclerosis (ALS) is a motor neuron disease characterized by the loss of motor neurons and the presence of protein inclusion aggregates containing TDP-43. Bimolecular fluorescence complementation (BiFC) combined with the zebrafish system can be used to visualize and analyze ALS-linked aggregates formation effectively. This approach holds promise for studying ALS pathology and potentially other neurodegenerative diseases associated with protein aggregation.
MOLECULAR NEUROBIOLOGY
(2021)
Article
Biochemical Research Methods
Shintaro Ichikawa, Shota Kato, Yuta Fujii, Kazuya Ishikawa, Keiji Numata, Yutaka Kodama
Summary: In this study, we artificially controlled chloroplast-chloroplast interactions in living plant cells using the organelle glue (ORGL) technique. By targeting different fragments of a fluorescent protein to specific locations, we modulated the frequency of chloroplast-chloroplast interactions.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Plant Sciences
Miao Fang, Jisuk Yu, Hae-Ryun Kwak, Kook-Hyung Kim
Summary: This study investigates the symptom determinants and viral protein functions of Pepper mottle virus (PepMoV) in different isolates. The research identifies key regions involved in virus accumulation and symptom development, providing insights into the infection cycle and plant-virus interactions.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Feng-Bin Zhang, Shun-Xia Ji, Jin-Guang Yang, Xiao-Wei Wang, Wen-Hao Han
Summary: The study reveals the involvement of MYB transcription factors in plant defense against whiteflies. The expression of NbMYB genes was induced upon whitefly infestation, suggesting their role in plant defense response.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Virology
Naiqing Xu, Xinen Tang, Xin Wang, Miao Cai, Xiaowen Liu, Xiaolong Lu, Shunlin Hu, Min Gu, Jiao Hu, Ruyi Gao, Kaituo Liu, Yu Chen, Xiufan Liu, Xiaoquan Wang
Summary: This study found that the H9N2 subtype avian influenza virus has a high airborne transmissibility, while the H7N9 virus does not. The Hemagglutinin protein of the H9N2 virus was found to play a key role in replication, stability, and airborne transmission.
Article
Virology
Samar S. Ewies, Sabry M. Tamam, Ahmed S. Abdel-Moneim, Sherin R. Rouby
Summary: Contagious ecthyma (CE) is a highly contagious viral disease of sheep and goats worldwide. The study provided a clinical description of CE and screened for genetic variation in the B2L gene. Infected sheep exhibited anorexia and oral lesions, while inoculated chicken embryos showed pock lesions. The B2L gene was successfully amplified and found to be highly conserved.
Article
Virology
Yigal Farnoushi, Dan Heller, Avishai Lublin
Summary: In recent years, new variants of avian reovirus (ARV) have caused a variety of symptoms in chickens worldwide, including viral arthritis/tenosynovitis. This study analyzed emerging ARV variants in Israel and found significant genetic diversity. Most ARV isolates in Israel belonged to genotypic cluster 5 (GC5). The study suggests that Israel has not experienced the emergence of new ARV variants since the introduction of the live vaccine (ISR-7585), but ongoing monitoring is needed due to the continuous emergence of ARV variants.
Article
Virology
Shigeru Tajima, Michiyo Kataoka, Yuki Takamatsu, Hideki Ebihara, Chang-Kweng Lim
Summary: Yokose virus (YOKV), a bat-associated flavivirus, was found to replicate at a slower rate in mosquito cells compared to other mosquito-borne flaviviruses. Specific nucleotide mutations in the virus were identified to enhance its proliferation ability in mosquito cells.
Article
Virology
Alejandra Borjabad, Baojun Dong, Wei Chao, David J. Volsky, Mary Jane Potash
Summary: This study investigated HIV brain disease using a mouse model, and found that poly I:C can reverse associated cognitive impairment and reduce virus burden. The results also revealed transcriptional changes related to neuronal function and innate immune responses.
Article
Virology
Ching-Hung Lin, Feng-Cheng Hsieh, Meilin Wang, Chieh Hsu, Hsuan-Wei Hsu, Chun-Chun Yang, Cheng-Yao Yang, Hung-Yi Wu
Summary: This study demonstrates that the synthesis of coronavirus subgenomic mRNA is not solely determined by the sequence homology between the leader TRS and TRS-B, but also by the disassociation of the coronavirus polymerase from the viral genome. This finding provides a new insight into the transcription mechanism of coronaviruses.
Article
Virology
Nicholas S. Kron, Benjamin W. Neuman, Sathish Kumar, Patricia L. Blackwelder, Dayana Vidal, Delphina Z. Walker-Phelan, Patrick D. I. Gibbs, Lynne A. Fieber, Michael C. Schmale
Summary: Two recent studies documented the genome of a novel virus in marine animals, finding that the virus is widespread in apparently healthy animals but not highly expressed in neurons. The studies also identified viral replication factories and high levels of defective genomes in chronically infected animals.
Article
Virology
Andrew M. Ramey, Laura C. Scott, Christina A. Ahlstrom, Evan J. Buck, Alison R. Williams, Mia Kim Torchetti, David E. Stallknecht, Rebecca L. Poulson
Summary: We successfully detected and characterized highly pathogenic avian influenza viruses in hunter-harvested wild waterfowl samples from western Alaska. Genomic analysis revealed three independent viral introductions into Alaska. Our findings demonstrate the utility and potential limitations of using molecular processing approaches directly on original swab samples for viral research and monitoring.
Article
Virology
Ting Gong, Dongdong Wu, Yongzhi Feng, Xing Liu, Qi Gao, Xiaoyu Zheng, Zebu Song, Heng Wang, Guihong Zhang, Lang Gong
Summary: This study discovered that quercetin can inhibit PEDV replication both in vivo and in vitro, and alleviate the clinical symptoms and intestinal injury caused by the virus. This provides a new direction for the development of PED antiviral drugs.
Article
Virology
Min Zhu, Hao Zeng, Jianqiao He, Yaohui Zhu, Pingping Wang, Jianing Guo, Jinfan Guo, Huabo Zhou, Yifeng Qin, Kang Ouyang, Zuzhang Wei, Weijian Huang, Ying Chen
Summary: The reassortment between avian H9N2 and Eurasian avian-like (EA) H1N1 viruses may have potentially changed from avian-to-mammals adaptation. This study found that the introduction of EA H1N1 internal genes into H9N2 virus restored the replication capability and resulted in extreme virulence in some cases. This raises new concerns for public health due to the possible coexistence of H9N2 and EA H1N1 viruses in dogs.