期刊
VIROLOGY
卷 456, 期 -, 页码 43-54出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2014.03.007
关键词
Tobamovirus; Movement protein; Cell-to-cell movement; Viral replication complex; Endoplasmic reticulum; Microtubules
类别
资金
- Grants of the Human Frontier Science Program (HSFP) [RGP 22/2006]
- Swiss National Science Foundation [310031A_140694]
Tobacco mosaic virus (TMV) is a longstanding model for studying virus movement and macromolecular transport through plasmodesmata (PD). Its movement protein (MP) interacts with cortical microtubule (MT)-associated ER sites (C-MERs) to facilitate the formation and transport of ER-associated viral replication complexes (VRCs) along the ER-actin network towards PD. To investigate whether this movement mechanism might be conserved between tobamoviruses, we compared the functions of Oilseed rape mosaic virus (ORMV) MP with those of MPTMV. We show that MPORMV supports TMV movement more efficiently than MPTMV. Moreover, MPORMV localizes to C-MERs like MPTMV but accumulates to lower levels and does not localize to larger inclusions/VRCs or along MTs, patterns regularly seen for MPTMV. Our findings extend the role of C-MERs in viral cell-to-cell transport to a virus commonly used for functional genomics in Arabidopsis. Moreover, accumulation of tobamoviral MP in inclusions or along MTs is not required for virus movement. (C) 2014 Elsevier Inc. All rights reserved.
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