期刊
VIROLOGY
卷 471, 期 -, 页码 29-37出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2014.09.022
关键词
JEV; TNF-alpha; Amniotic; Endothelial cells; NF kappa B mediated HLA-F induction
类别
资金
- Council for Scientific and Industrial Research (CSIR) [CSIR37(1436)/10/EMR-II]
- DBT, Government of India, Pathogen Biology and DBT-IISc Partnership Programs at the Indian Institute of Science, Bangalore [DBT/BF/PR/INS/2011-12/IISc]
- DBT
- CSIR, Govt of India
Productive infection of human amniotic and endothelial cell lines with Japanese encephalitis virus (JEV) was established leading to the induction of NF kappa B and HLA-F, a non-classical MHC molecule. Induction of the HLA-F gene and protein in JEV-infected cells was shown to be NF kappa B dependent since it was blocked by inhibitors of NF kappa B activation. ShRNA targeting lentivirus-mediated stable knockdown of the p65 subunit of NF kappa B inhibited JEV-mediated induction of HLA-F both in the amniotic cell line, AV-3 as well as the human brain microendothelial cell line, HBMEC. The induction of HLA-F by treatment of AV-3 with TNF-alpha was also inhibited by ShRNA mediated knockdown of NF kappa B. TNF-alpha treatment of HEK293T cells that were transfected with reporter plasmids under the control of HLA-F enhancer A elements resulted in significant transactivation of the luciferase reporter gene. NF kappa B-mediated induction of HLA-F following JEV infection and TNF-alpha exposure is being suggested for the first time. (C) 2014 Elsevier Inc. All rights reserved.
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