期刊
VIROLOGY
卷 452, 期 -, 页码 287-296出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2014.01.024
关键词
Geminiviridae; Begomovirus; Euphorbia mosaic virus; Cleome leaf crumple virus; Rolling circle amplification (RCA); Somatic homologous recombination; Arabidopsis thaliana
类别
资金
- DFG [Je116/15-1]
- ERA-PG program [BMBF 0313986]
Four transgenic Arabidopsis thaliana lines carrying different reporter gene constructs based on split glucuronidase genes were used to monitor the frequency of somatic homologous recombination after geminivirus infections. Euphorbia mosaic virus and Cleome leaf crumple virus were chosen as examples, because they induce only mild symptoms and are expected to induce less general stress responses than other geminiviruses. After comparing the different plant lines and viruses as well as optimizing the infection procedure, Euphorbia mosaic virus enhanced recombination rates significantly in the transgenic reporter line 1445. The effect was tissue-specific in cells of the leaf veins as expected for this phloem-limited virus. The advantage for geminiviruses to activate a general recombination pathway is discussed with reference to an increased fitness by generating virus recombinants which have been observed frequently as an epidemiologic driving force. (C) 2014 Elsevier Inc. All rights reserved.
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