Article
Biochemistry & Molecular Biology
Kevin Delgado-Cunningham, Tomas Lopez, Firas Khatib, Carlos F. Arias, Rebecca M. DuBois
Summary: This study reports the structure of the capsid protein spike of the HAstV MLB clade and reveals its significant differences compared to the classical HAstV spikes. The study also suggests that MLB and classical HAstVs may utilize different receptors for host-cell attachment. Furthermore, the study shows that template-based predictions are surprisingly accurate relative to machine-learning-based predictions.
Article
Virology
Woonghee Lee, Ronnie O. Frederick, Marco Tonelli, Ann C. Palmenberg
Summary: CDHR3 is the cellular receptor for all virus isolates in the rhinovirus-C species (RV-C), with its extracellular domain 1 (EC1) binding to a hypervariable sequence footprint on the virus surface. The solution NMR determination of CDHR3 EC1 has shown that the protein is mostly inflexible, particularly in the virus-binding face, with cis-Pro residues influencing rigidity and overall conformation. The higher resolution dataset clarifies residues involved in essential calcium ion binding and a potential CDHR3 surface groove feature for native protein interactions with cellular partners.
Article
Biotechnology & Applied Microbiology
Matthew D. J. Dicks, Louisa M. Rose, Rebecca A. Russell, Lesley A. H. Bowman, Carl Graham, Jose M. Jimenez-Guardeno, Katie J. Doores, Michael H. Malim, Simon J. Draper, Mark Howarth, Sumi Biswas
Summary: This study describes a system that allows for the display of antigens on the surface of adenovirus vectors, resulting in potent humoral immunity. By using a protein superglue, antigens can be attached to the adenovirus capsid, improving the efficacy and boostability of the vaccine. Capsid decoration shields the virus particles from neutralizing antibodies.
Article
Biochemistry & Molecular Biology
Martin Selinger, Radim Novotny, Jakub Sys, Justin A. Roby, Hana Tykalova, Ganji Sri Ranjani, Marie Vancova, Katerina Jaklova, Filip Kaufman, Marshall E. Bloom, Zbynek Zdrahal, Libor Grubhoffer, Jade K. Forwood, Richard Hrabal, Michaela Rumlova, Jan Sterba
Summary: Tick-borne encephalitis virus (TBEV) is a medically significant flavivirus transmitted by ticks in Eurasia. It primarily affects the host's central nervous system, leading to severe encephalitis. The capsid protein (TBEVC) plays a critical role in host recognition and viral replication.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Nianxiang Wang, Zi Wang, Mengyao Ma, Xinhao Jia, Hang Liu, Mengwei Qian, Sijia Lu, Yuqiang Xiang, Zhanyong Wei, Lanlan Zheng
Summary: Porcine deltacoronavirus (PDCoV), a global epidemic enteropathogenic coronavirus, mainly infects piglets and causes significant losses to the pig industry. Despite the lack of commercial vaccines for PDCoV prevention and control, this study successfully analyzed the receptor-binding domain (RBD) of PDCoV and developed a vaccine using the RBD protein as a protective antigen. The vaccine induced stronger immune responses in mice and provided better protection against PDCoV compared to other adjuvants tested.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Immunology
Kizzmekia S. Corbett, Anne P. Werner, Sarah O' Connell, Matthew Gagne, Lilin Lai, Juan Moliva, Barbara Flynn, Angela Choi, Matthew Koch, Kathryn E. Foulds, Shayne F. Andrew, Dillon R. Flebbe, Evan Lamb, Saule T. Nurmukhambetova, Samantha J. Provost, Kevin W. Bock, Mahnaz Minai, Bianca M. Nagata, Alex Van Ry, Zackery Flinchbaugh, Timothy S. Johnston, Elham Bayat Mokhtari, Prakriti Mudvari, Amy R. Henry, Farida Laboune, Becky Chang, Maciel Porto, Jaclyn Wear, Gabriela S. Alvarado, Seyhan Boyoglu-Barnum, John-Paul M. Todd, Bridget Bart, Anthony Cook, Alan Dodson, Laurent Pessaint, Katelyn Steingrebe, Sayda Elbashir, Manjari Sriparna, Andrew Pekosz, Hanne Andersen, Kai Wu, Darin K. Edwards, Swagata Kar, Mark G. Lewis, Eli Boritz, Ian N. Moore, Andrea Carfi, Mehul S. Suthar, Adrian McDermott, Mario Roederer, Martha C. Nason, Nancy J. Sullivan, Daniel C. Douek, Barney S. Graham, Robert A. Seder
Summary: The mRNA-1273 vaccine from Moderna in a two-dose regimen provides strong protection against major variants of concern, particularly the B.1.351 variant. Higher neutralizing antibody responses and reduced viral replication in the upper and lower airways were observed after two doses, highlighting the importance of the full vaccine regimen for effective immunity.
Article
Virology
Luis Valiente, Silvia Lopez-Arguello, Alicia Rodriguez-Huete, Alejandro Valbuena, Mauricio G. Mateu
Summary: This study investigates the specific residues along the capsid interpentamer interfaces that are relevant to HRV infection and the viral cycle. The results suggest that these residues play a critical role in virion assembly and stability and therefore hold potential as targets for antiviral drugs. The study highlights the importance of understanding the structure-function relationship in HRV for the development of effective anti-HRV agents.
JOURNAL OF VIROLOGY
(2022)
Article
Cell Biology
Chingakham Ranjit Singh, M. Rebecca Glineburg, Chelsea Moore, Naoki Tani, Rahul Jaiswal, Ye Zou, Eric Aube, Sarah Gillaspie, Mackenzie Thornton, Ariana Cecil, Madelyn Hilgers, Azuma Takasu, Izumi Asano, Masayo Asano, Carlos R. Escalante, Akira Nakamura, Peter K. Todd, Katsura Asano
Summary: The eIF5-mimic protein (5MP) has been found to suppress non-AUG translation by interacting with eIF3, increasing the accuracy of initiation and protecting cells from unwanted byproducts of non-AUG translation in neurodegeneration.
Article
Multidisciplinary Sciences
Dominik Hrebik, Tibor Fuzik, Maria Gondova, Lenka Smerdova, Athanassios Adamopoulos, Ondrej Sedo, Zbynek Zdrahal, Pavel Plevka
Summary: This study elucidated the structure of the rhinovirus 14 complex with ICAM-1, showing how this complex is crucial for virus activation and genome release. The binding of ICAM-1 induces conformational changes in the capsid of rhinovirus 14, priming it for activation and genome release under acidic pH conditions.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Yusuke Higuchi, Tatsuya Suzuki, Takao Arimori, Nariko Ikemura, Emiko Mihara, Yuhei Kirita, Eriko Ohgitani, Osam Mazda, Daisuke Motooka, Shota Nakamura, Yusuke Sakai, Yumi Itoh, Fuminori Sugihara, Yoshiharu Matsuura, Satoaki Matoba, Toru Okamoto, Junichi Takagi, Atsushi Hoshino
Summary: The engineered human virus receptor ACE2 successfully neutralized SARS-CoV-2 at a lower concentration, preventing escape mutants from emerging. Furthermore, therapeutic administration of engineered ACE2 protected hamsters from infection and decreased lung virus titers and pathology.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Jennifer Gerbracht, Tommy Harding, Alastair G. B. Simpson, Andrew J. Roger, Sebastian Hess
Summary: This study used the flagellate Orciraptor agilis as a model protoplast feeder to examine its penetration of green algal cell walls and discovered lytic carbohydrate-active enzymes that are highly expressed and upregulated during the attack on the alga. Unexpectedly, various chitin-related factors indicating an unknown chitin metabolism in Orciraptor agilis were also found, suggesting their potential involvement in the feeding process. These findings provide molecular insights into microbial feeding behaviors and open up new directions for research on non-model eukaryotes in cell biology.
Article
Virology
Naoyuki Miyazaki, Chihong Song, Tomoichiro Oka, Motohiro Miki, Kosuke Murakami, Kenji Iwasaki, Kazuhiko Katayama, Kazuyoshi Murata
Summary: SaV and norovirus are common causes of acute gastroenteritis in humans and animals, posing public health problems worldwide. The genetically diverse HuSaV has hindered the development of vaccines and therapeutics due to the lack of viral replication system and structural information. This study successfully produced a virus-like particle from HuSaV and determined its atomic structure, providing important insights for medical treatment and vaccine development.
JOURNAL OF VIROLOGY
(2022)
Review
Biochemistry & Molecular Biology
S. Zolotukhin, L. H. Vandenberghe
Summary: In vivo therapeutic gene transfer is a novel class of medicines that relies on the safe and effective delivery of genetic material. The adeno-associated virus (AAV) vector has emerged as a preferred gene delivery vehicle, allowing for therapeutic gene expression in various clinical indications. Recent trends in AAV capsid engineering focus on enhancing targeting specificity, safety, and endurance, a significant challenge that is being addressed through techniques such as directed evolution, sequence analysis, and machine learning.
TRENDS IN MOLECULAR MEDICINE
(2022)
Article
Multidisciplinary Sciences
Hyunbum Jang, Iris Nira Smith, Charis Eng, Ruth Nussinov
Summary: The research used explicit solvent simulations to uncover how PTEN is localized on the cell membrane and inhibits cancer. By observing the interaction of PTEN with different components of the cell membrane, the study revealed its localization on signaling lipids and its effect on PIP3.
Article
Biochemical Research Methods
Trevor J. Gonzalez, Aaron Mitchell-Dick, Leo O. Blondel, Marco M. Fanous, Joshua A. Hull, Daniel K. Oh, Sven Moller-Tank, Ruth M. Castellanos Rivera, Jorge A. Piedrahita, Aravind Asokan
Summary: This article describes a structure-guided library approach to evolve new AAV capsids with improved characteristics. The method can enhance gene transfer efficiency, immune evasion, and can be adapted for genetic manipulation in animal models and development of human gene therapies.
Article
Virology
Holly A. Basta, Sean B. Cleveland, Rochelle A. Clinton, Alexander G. Dimitrov, Marcella A. McClure
JOURNAL OF VIROLOGY
(2009)
Article
Virology
Holly A. Basta, Valjean R. Bacot-Davis, Jessica J. Ciomperlik, Ann C. Palmenberg
JOURNAL OF VIROLOGY
(2014)
Article
Biochemistry & Molecular Biology
Peter A. Novick, Holly Basta, Mark Floumanhaft, Marcella A. McClure, Stephane Boissinot
MOLECULAR BIOLOGY AND EVOLUTION
(2009)
Article
Virology
Holly A. Basta, Shamaila Ashraf, Jean-Yves Sgro, Yury A. Bochkov, James E. Gern, Ann C. Palmenberg
Article
Virology
Holly A. Basta, Ann C. Palmenberg
Article
Multidisciplinary Sciences
Alaguraj Veluchamy, Xin Lin, Florian Maumus, Maximo Rivarola, Jaysheel Bhavsar, Todd Creasy, Kimberly O'Brien, Naomi A. Sengamalay, Luke J. Tallon, Andrew D. Smith, Edda Rayko, Ikhlak Ahmed, Stephane Le Crom, Gregory K. Farrant, Jean-Yves Sgro, Sue A. Olson, Sandra Splinter Bondurant, Andrew Allen, Pablo D. Rabinowicz, Michael R. Sussman, Chris Bowler, Leila Tirichine
NATURE COMMUNICATIONS
(2013)
Letter
Dermatology
Rubab Raza, Gagan Chhabra, Muhammad Bilal, Mary A. Ndiaye, Khurram Liaqat, Shoaib Nawaz, Jean-Yves Sgro, Ivan Rayment, Wasim Ahmad, Nihal Ahmad
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2023)
Article
Evolutionary Biology
Holly A. Basta, Alex J. Buzak, Marcella A. McClure
EVOLUTIONARY BIOINFORMATICS
(2007)
Article
Evolutionary Biology
Sivan Vadakkadath Meethal, Miguel J. Gallego, Ryan J. Haasl, Stephen J. Petras, Jean-Yves Sgro, Craig S. Atwood
BMC EVOLUTIONARY BIOLOGY
(2006)
Article
Virology
AA Khromykh, N Kondratieva, JY Sgro, A Palmenberg, EG Westaway
JOURNAL OF VIROLOGY
(2003)
Article
Virology
K Hueffer, JSL Parker, WS Weichert, RE Geisel, JY Sgro, CR Parrish
JOURNAL OF VIROLOGY
(2003)
Article
Virology
Yuri Kusov, Tatsuo Kanda, Ann Palmenberg, Jean-Yves Sgro, Verena Gauss-Mueller
JOURNAL OF VIROLOGY
(2006)
Article
Virology
Naiqing Xu, Xinen Tang, Xin Wang, Miao Cai, Xiaowen Liu, Xiaolong Lu, Shunlin Hu, Min Gu, Jiao Hu, Ruyi Gao, Kaituo Liu, Yu Chen, Xiufan Liu, Xiaoquan Wang
Summary: This study found that the H9N2 subtype avian influenza virus has a high airborne transmissibility, while the H7N9 virus does not. The Hemagglutinin protein of the H9N2 virus was found to play a key role in replication, stability, and airborne transmission.
Article
Virology
Samar S. Ewies, Sabry M. Tamam, Ahmed S. Abdel-Moneim, Sherin R. Rouby
Summary: Contagious ecthyma (CE) is a highly contagious viral disease of sheep and goats worldwide. The study provided a clinical description of CE and screened for genetic variation in the B2L gene. Infected sheep exhibited anorexia and oral lesions, while inoculated chicken embryos showed pock lesions. The B2L gene was successfully amplified and found to be highly conserved.
Article
Virology
Yigal Farnoushi, Dan Heller, Avishai Lublin
Summary: In recent years, new variants of avian reovirus (ARV) have caused a variety of symptoms in chickens worldwide, including viral arthritis/tenosynovitis. This study analyzed emerging ARV variants in Israel and found significant genetic diversity. Most ARV isolates in Israel belonged to genotypic cluster 5 (GC5). The study suggests that Israel has not experienced the emergence of new ARV variants since the introduction of the live vaccine (ISR-7585), but ongoing monitoring is needed due to the continuous emergence of ARV variants.
Article
Virology
Shigeru Tajima, Michiyo Kataoka, Yuki Takamatsu, Hideki Ebihara, Chang-Kweng Lim
Summary: Yokose virus (YOKV), a bat-associated flavivirus, was found to replicate at a slower rate in mosquito cells compared to other mosquito-borne flaviviruses. Specific nucleotide mutations in the virus were identified to enhance its proliferation ability in mosquito cells.
Article
Virology
Alejandra Borjabad, Baojun Dong, Wei Chao, David J. Volsky, Mary Jane Potash
Summary: This study investigated HIV brain disease using a mouse model, and found that poly I:C can reverse associated cognitive impairment and reduce virus burden. The results also revealed transcriptional changes related to neuronal function and innate immune responses.
Article
Virology
Ching-Hung Lin, Feng-Cheng Hsieh, Meilin Wang, Chieh Hsu, Hsuan-Wei Hsu, Chun-Chun Yang, Cheng-Yao Yang, Hung-Yi Wu
Summary: This study demonstrates that the synthesis of coronavirus subgenomic mRNA is not solely determined by the sequence homology between the leader TRS and TRS-B, but also by the disassociation of the coronavirus polymerase from the viral genome. This finding provides a new insight into the transcription mechanism of coronaviruses.
Article
Virology
Nicholas S. Kron, Benjamin W. Neuman, Sathish Kumar, Patricia L. Blackwelder, Dayana Vidal, Delphina Z. Walker-Phelan, Patrick D. I. Gibbs, Lynne A. Fieber, Michael C. Schmale
Summary: Two recent studies documented the genome of a novel virus in marine animals, finding that the virus is widespread in apparently healthy animals but not highly expressed in neurons. The studies also identified viral replication factories and high levels of defective genomes in chronically infected animals.
Article
Virology
Andrew M. Ramey, Laura C. Scott, Christina A. Ahlstrom, Evan J. Buck, Alison R. Williams, Mia Kim Torchetti, David E. Stallknecht, Rebecca L. Poulson
Summary: We successfully detected and characterized highly pathogenic avian influenza viruses in hunter-harvested wild waterfowl samples from western Alaska. Genomic analysis revealed three independent viral introductions into Alaska. Our findings demonstrate the utility and potential limitations of using molecular processing approaches directly on original swab samples for viral research and monitoring.
Article
Virology
Ting Gong, Dongdong Wu, Yongzhi Feng, Xing Liu, Qi Gao, Xiaoyu Zheng, Zebu Song, Heng Wang, Guihong Zhang, Lang Gong
Summary: This study discovered that quercetin can inhibit PEDV replication both in vivo and in vitro, and alleviate the clinical symptoms and intestinal injury caused by the virus. This provides a new direction for the development of PED antiviral drugs.
Article
Virology
Min Zhu, Hao Zeng, Jianqiao He, Yaohui Zhu, Pingping Wang, Jianing Guo, Jinfan Guo, Huabo Zhou, Yifeng Qin, Kang Ouyang, Zuzhang Wei, Weijian Huang, Ying Chen
Summary: The reassortment between avian H9N2 and Eurasian avian-like (EA) H1N1 viruses may have potentially changed from avian-to-mammals adaptation. This study found that the introduction of EA H1N1 internal genes into H9N2 virus restored the replication capability and resulted in extreme virulence in some cases. This raises new concerns for public health due to the possible coexistence of H9N2 and EA H1N1 viruses in dogs.
