期刊
VIROLOGY
卷 444, 期 1-2, 页码 158-170出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2013.06.004
关键词
Baculovirus; HearNPV; Helicoverpa; Heliothis; Virus-host interactions; Insect virus; Microarray; Genome scale; Expression profile
类别
资金
- Australian Research Council [LP0989824]
- Agrichem Pty Ltd.
- University of Queensland
- Australian Research Council [LP0989824] Funding Source: Australian Research Council
Knowledge of baculovirus insect host interactions at a genome-scale level is important for developing a number of baculovirus-based applications, but the gathering of such knowledge is hindered by the lack of genomic sequences in most insect hosts. In this study, expression kinetics of 24,206 Helicoveipa zea insect transcripts and 134 Helicoveipa armigera nucleopolyhedrovirus (HearNPV) genes at 0, 12, 24 and 48 h post-infection (hpi) were simultaneously analyzed using microarrays, which were developed from sequences obtained by deep transcriptome sequencing. Host genes in pathways important for infection such as those for energy generation, anti-viral peptides, apoptosis, detoxification, DNA polymerase activities, RNA polymerase activities, translation initiation, protein processing and cell cycle arrest were identified. Differential expression was linked to changes in the number of intracellular and extracellular viral genomes and occlusion bodies. The first comprehensive elucidation of HearNPV H. zea expression kinetics was obtained. (C) 2013 Elsevier Inc. All rights reserved.
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