期刊
VIROLOGY
卷 447, 期 1-2, 页码 131-139出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2013.09.001
关键词
Rice black-streaked dwarf virus; Capsid protein P10; ER membrane modification; ER stress; Unfolded protein response
类别
资金
- State Basic Research Program of China [2014CB138403, 2010CB126203, 2012CB722504]
- International Science & Technology Cooperation Program of China [2012DFA30900]
- National Natural Science Foundation of China [31071660, 31301637]
- Zhejiang Provincial Natural Science Foundation of China [Q13C140009]
- Zhejiang Provincial Postdoctoral Research Project Preferential Foundation [Bsh1202085]
- Program for Zhejiang Leading Team of Science and Technology Innovation [2009R50032]
- Program for Leading Team of Agricultural Research and Innovation of Ministry of Agriculture, China
Endoplasmic reticular (ER) membrane modifications play an important role in viral RNA replication and virion assembly but little is known about the involvement of ER-membrane remodeling in the infection cycle of fijiviruses in plant cells. The subcellular localization of Rice black-streaked dwarf virus outer capsid P10 was therefore examined using live-cell imaging. P10 fused to eGFP formed vesicular structures associated with ER membranes in Nicotiana benthamiana epidermal cells and in rice protoplasts. Subcellular fractionation experiments confirmed that P10 is an integral membrane protein. Three predicted transmembrane domains and two less-well-defined domains were each able to target eGFP to the ER. Disruption of the actin cytoskeleton with LatB, indicated that the maintenance of P10-induced membrane structures required the intact actin cytoskeleton. P10 induced the expression of ER stress marker genes, including ER stress-related chaperones and transcription factor, indicating that RBSDV P10 triggers ER stress and the unfolded protein response. (C) 2013 Elsevier Inc. All rights reserved.
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