Article
Oncology
Lucia Oton-Gonzalez, John Charles Rotondo, Carmen Lanzillotti, Elisa Mazzoni, Ilaria Bononi, Maria Rosa Iaquinta, Luca Cerritelli, Nicola Malagutti, Andrea Ciorba, Chiara Bianchini, Stefano Pelucchi, Mauro Tognon, Fernanda Martini
Summary: Classical markers alone may not be enough to classify HPV-positive HNSCC patients, but when combined with serological markers, they are strong prognostic indicators in OPSCC patients. Detection of HPV16 E7 oncoprotein in serum at diagnosis is correlated with disease recurrence and patient overall survival, providing a useful tool for stratifying and monitoring HPV-positive HNSCC patients for worse prognosis.
Article
Oncology
Xinmei Wang, Guangnan Shuai, Junhui Xu, Meihua Liu, Jianguo Zhao, Na Zhang, Wenwen Zhang, Pengpeng Qu
Summary: This study investigated the application of quantitative HPV16 E7 oncoprotein detection in cervical cancer screening. The results showed a positive correlation between the level of HPV16 E7 oncoprotein and the degree of cervical lesions, with a cut-off value of 8.68 ng/ml for identifying HSIL+. This method demonstrated good diagnostic value and predictive ability for disease progression.
FRONTIERS IN ONCOLOGY
(2023)
Article
Oncology
Leticia Torres-Ibarra, Attila T. Lorincz, Cosette M. Wheeler, Jack Cuzick, Rubi Hernandez-Lopez, Donna Spiegelman, Leith Leon-Maldonado, Berenice Rivera-Paredez, Pablo Mendez-Hernandez, Eduardo Lazcano-Ponce, Jorge Salmeron
Summary: The study demonstrates that adjunctive testing of HPV16/18-positive women with DS can greatly reduce unnecessary colposcopy referrals while maintaining acceptable sensitivity for CIN2+ and CIN3+.
INTERNATIONAL JOURNAL OF CANCER
(2021)
Article
Multidisciplinary Sciences
Sheung Chun Ng, Abin Biswas, Trevor Huyton, Juergen Schuenemann, Simone Reber, Dirk Goerlich
Summary: The Nup98 FG repeat domains in the nuclear pore complex (NPC) capture nuclear transport receptors (NTRs) and condense into a selective phase with barrier properties. Shortening the FG spacers enhances phase cohesion and barrier tightness, while arginines promote phase entry through hydrophobic interactions/hydrogen-bonding. Mutations in the FG motifs affect NTR entry, and the antagonism between cohesion and FG interactions is crucial for transport selectivity.
NATURE COMMUNICATIONS
(2023)
Article
Microbiology
Wenchao Ai, Chuanyong Wu, Liqing Jia, Xiao Xiao, Xuewen Xu, Min Ren, Tian Xue, Xiaoyan Zhou, Ying Wang, Chunfang Gaoa
Summary: This study provides evidence for the first time that HPV16 E6 sequences contain vital mutation features in predicting high-grade squamous intraepithelial lesion and can reduce even more unneeded colposcopies without a loss of sensitivity to detect cervical cancer. Moreover, the D32E and H85Y variants of E6 exhibited a significantly higher ability to degrade p53, which may play a vital role in the development of cervical cancer.
MICROBIOLOGY SPECTRUM
(2022)
Article
Obstetrics & Gynecology
Yi-Pin Chen, Chu-Chi Lin, Yu-Xin Xie, Chia-Yuan Chen, J. Timothy Qiu
Summary: The study linked codon-optimized GM-CSF to the HPV16 E7 sequence in a DNA vaccine to enhance immunity against tumor challenges. Results showed that the cGM-CSF-E7 DNA vaccine slowed tumor growth rate and promoted secretion of IFN-g by CD4 and CD8+ T cells. In vivo antibody depletion experiments demonstrated the important role of both CD4+ and CD8+ T cells in suppressing tumor growth.
TAIWANESE JOURNAL OF OBSTETRICS & GYNECOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Chengyu Hao, Yunji Zheng, Johanna Jonsson, Xiaoxu Cui, Haoran Yu, Chengjun Wu, Naoko Kajitani, Stefan Schwartz
Summary: By investigating the expression regulation of Human papillomavirus type 16 (HPV16) E2, a required splicing enhancer has been identified, which interacts with cellular RNA binding protein hnRNP G and promotes splicing and production of E2 mRNA. The splicing-enhancing function of hnRNP G is mediated by its interaction with splicing factor U2AF65. The interaction between hnRNP G and HPV16 E2 mRNA and U2AF65 is increased by keratinocyte differentiation and DNA damage response, while sumoylation of hnRNP G is reduced under the DNA damage response, leading to increased splicing and interactions between hnRNP G and E2 mRNA as well as U2AF65. Moreover, hnRNP G inhibits the splicing of the HPV16 E6 coding region, thereby suppressing the production of spliced E7 oncogene mRNAs.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Chemistry, Multidisciplinary
Susanna Falcucci, Francesca Paolini, Anna Maria Mileo, Rosella Franconi, Silvia Massa, Antonio Rinaldi, Aldo Venuti
Summary: This study successfully demonstrated the feasibility of immobilizing HPV16 E7 onto electrospun substrates for diagnostic tools, increasing detection signal from serum antibodies. The new anti-E7 ePCL-ELISA test showed higher efficiency in detecting E7-specific antibodies and can be prepared with a relatively low amount of antigen, reducing manufacturing costs.
Article
Microbiology
Shiwen Peng, Deyin Xing, Louise Ferrall, Ya-Chea Tsai, Richard B. S. Roden, Chien-Fu Hung, T. -C. Wu
Summary: Our research focused on eliminating the confounding effects of dominant HPV antigen presentation through murine MHC-I via epitope mutagenesis, while ensuring oncogenicity. Mutated DNA constructs of HPV16 E6 and E7 were able to abolish murine MHC-I presentation and instead use human HLA-A2 molecules for presentation. These mutated constructs still maintained their oncogenic properties.
Article
Microbiology
Shiwen Peng, Deyin Xing, Louise Ferrall, Ya-Chea Tsai, Richard B. S. Roden, Chien-Fu Hung, T-C Wu
Summary: The study developed a spontaneous HPV16-driven HNSCC model in HLA-A2 transgenic mice to test candidate immunotherapies. They eliminated the confounding effects of dominant HPV antigen presentation through epitope mutagenesis and characterized their presentation in murine and human MHC-I molecules.
Article
Chemistry, Multidisciplinary
Susanna Falcucci, Francesca Paolini, Anna Maria Mileo, Rosella Franconi, Silvia Massa, Antonio Rinaldi, Aldo Venuti
Summary: This study demonstrates the feasibility of immobilizing HPV16 E7 onto electrospun substrates for diagnostic tools. By functionalizing ePCL with E7, it showed superior properties compared to standard polystyrene plates, increasing the detection signal from serum antibodies by 5-6 times.
Article
Multidisciplinary Sciences
Eszter E. Najbauer, Sheung Chun Ng, Christian Griesinger, Dirk Goerlich, Loren B. Andreas
Summary: The cohesive FG domains in nuclear pore complexes form a selective permeability barrier, with nanoscopic insight into their interactions hindered by sequence heterogeneity. By using an engineered repetitive sequence and NMR spectroscopy, researchers were able to overcome this challenge and map the dynamics of cohesive interactions. The study found that the FG repeats remained disordered in both phase-separated and soluble states, with slow translation observed in phase-separated domains.
NATURE COMMUNICATIONS
(2022)
Article
Immunology
Shuang Ding, Hanmeng Wang, Yaqi Liao, Ranzhong Chen, Yu Hu, Hongrong Wu, Haiyan Shen, Shuangyang Tang
Summary: HPV16 E7 protein interacts with Daxx, possibly affecting the progression of cervical cancer, and the Daxx/JNK pathway may be involved in the anti-apoptotic activity of HPV16 E7.
MICROBIAL PATHOGENESIS
(2023)
Article
Biochemistry & Molecular Biology
Yazmin Gomez-Gomez, Jorge Organista-Nava, Sayuri Itzel Clemente-Perivan, Alfredo Lagunas-Martinez, Eric Genaro Salmeron-Barcenas, Daniel Villanueva-Morales, Dania Yahaira Ayala-Reyna, Luz del Carmen Alarcon-Romero, Julio Ortiz-Ortiz, Marco Antonio Jimenez-Lopez, Ciresthel Bello-Rios, Marco Antonio Leyva-Vazquez, Berenice Illades-Aguiar
Summary: The transcription factor Oct3/4 is involved in maintaining cancer stem cell characteristics. This study found that the expression of Oct3/4 varies with the progression of cervical cancer. Additionally, Oct3/4 can give rise to three isoforms through alternative splicing. The research showed that the expression of Oct3/4A, Oct3/4B, and Oct3/4B1 is significantly higher in patients with low-grade squamous intraepithelial lesion (LSIL), high-grade squamous intraepithelial lesion (HSIL), and cervical cancer (CC). Furthermore, the presence of HPV16 E7 oncoprotein increased the expression of Oct3/4A mRNA.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Xuechao Han, Zhixiao Gao, Yeping Cheng, Shuoshuo Wu, Jianxing Chen, Weifang Zhang
Summary: The study developed a vaccine targeting HPV E7 and demonstrated that combination therapy with immune checkpoint blockade effectively inhibited tumor growth and improved control of cervical cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Virology
Naiqing Xu, Xinen Tang, Xin Wang, Miao Cai, Xiaowen Liu, Xiaolong Lu, Shunlin Hu, Min Gu, Jiao Hu, Ruyi Gao, Kaituo Liu, Yu Chen, Xiufan Liu, Xiaoquan Wang
Summary: This study found that the H9N2 subtype avian influenza virus has a high airborne transmissibility, while the H7N9 virus does not. The Hemagglutinin protein of the H9N2 virus was found to play a key role in replication, stability, and airborne transmission.
Article
Virology
Samar S. Ewies, Sabry M. Tamam, Ahmed S. Abdel-Moneim, Sherin R. Rouby
Summary: Contagious ecthyma (CE) is a highly contagious viral disease of sheep and goats worldwide. The study provided a clinical description of CE and screened for genetic variation in the B2L gene. Infected sheep exhibited anorexia and oral lesions, while inoculated chicken embryos showed pock lesions. The B2L gene was successfully amplified and found to be highly conserved.
Article
Virology
Yigal Farnoushi, Dan Heller, Avishai Lublin
Summary: In recent years, new variants of avian reovirus (ARV) have caused a variety of symptoms in chickens worldwide, including viral arthritis/tenosynovitis. This study analyzed emerging ARV variants in Israel and found significant genetic diversity. Most ARV isolates in Israel belonged to genotypic cluster 5 (GC5). The study suggests that Israel has not experienced the emergence of new ARV variants since the introduction of the live vaccine (ISR-7585), but ongoing monitoring is needed due to the continuous emergence of ARV variants.
Article
Virology
Shigeru Tajima, Michiyo Kataoka, Yuki Takamatsu, Hideki Ebihara, Chang-Kweng Lim
Summary: Yokose virus (YOKV), a bat-associated flavivirus, was found to replicate at a slower rate in mosquito cells compared to other mosquito-borne flaviviruses. Specific nucleotide mutations in the virus were identified to enhance its proliferation ability in mosquito cells.
Article
Virology
Alejandra Borjabad, Baojun Dong, Wei Chao, David J. Volsky, Mary Jane Potash
Summary: This study investigated HIV brain disease using a mouse model, and found that poly I:C can reverse associated cognitive impairment and reduce virus burden. The results also revealed transcriptional changes related to neuronal function and innate immune responses.
Article
Virology
Ching-Hung Lin, Feng-Cheng Hsieh, Meilin Wang, Chieh Hsu, Hsuan-Wei Hsu, Chun-Chun Yang, Cheng-Yao Yang, Hung-Yi Wu
Summary: This study demonstrates that the synthesis of coronavirus subgenomic mRNA is not solely determined by the sequence homology between the leader TRS and TRS-B, but also by the disassociation of the coronavirus polymerase from the viral genome. This finding provides a new insight into the transcription mechanism of coronaviruses.
Article
Virology
Nicholas S. Kron, Benjamin W. Neuman, Sathish Kumar, Patricia L. Blackwelder, Dayana Vidal, Delphina Z. Walker-Phelan, Patrick D. I. Gibbs, Lynne A. Fieber, Michael C. Schmale
Summary: Two recent studies documented the genome of a novel virus in marine animals, finding that the virus is widespread in apparently healthy animals but not highly expressed in neurons. The studies also identified viral replication factories and high levels of defective genomes in chronically infected animals.
Article
Virology
Andrew M. Ramey, Laura C. Scott, Christina A. Ahlstrom, Evan J. Buck, Alison R. Williams, Mia Kim Torchetti, David E. Stallknecht, Rebecca L. Poulson
Summary: We successfully detected and characterized highly pathogenic avian influenza viruses in hunter-harvested wild waterfowl samples from western Alaska. Genomic analysis revealed three independent viral introductions into Alaska. Our findings demonstrate the utility and potential limitations of using molecular processing approaches directly on original swab samples for viral research and monitoring.
Article
Virology
Ting Gong, Dongdong Wu, Yongzhi Feng, Xing Liu, Qi Gao, Xiaoyu Zheng, Zebu Song, Heng Wang, Guihong Zhang, Lang Gong
Summary: This study discovered that quercetin can inhibit PEDV replication both in vivo and in vitro, and alleviate the clinical symptoms and intestinal injury caused by the virus. This provides a new direction for the development of PED antiviral drugs.
Article
Virology
Min Zhu, Hao Zeng, Jianqiao He, Yaohui Zhu, Pingping Wang, Jianing Guo, Jinfan Guo, Huabo Zhou, Yifeng Qin, Kang Ouyang, Zuzhang Wei, Weijian Huang, Ying Chen
Summary: The reassortment between avian H9N2 and Eurasian avian-like (EA) H1N1 viruses may have potentially changed from avian-to-mammals adaptation. This study found that the introduction of EA H1N1 internal genes into H9N2 virus restored the replication capability and resulted in extreme virulence in some cases. This raises new concerns for public health due to the possible coexistence of H9N2 and EA H1N1 viruses in dogs.