Article
Biochemistry & Molecular Biology
Kaushik Saha, Gourisankar Ghosh
Summary: This study reveals a novel function of U1 snRNP in defining splicing substrates in mammals. It demonstrates how SR proteins and U1 snRNP cooperate to generate a substrate for spliceosome assembly. The excess U1 snRNP selectively displaces some SR proteins, allowing for sequential recognition of splice signals. This mechanism may be employed by other splicing regulatory proteins.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Adi Amar-Schwartz, Yuval Cohen, Antony Elhaj, Vered Ben-Hur, Zahava Siegfried, Rotem Karni, Talya Dor
Summary: Duchene muscular dystrophy (DMD) and Becker muscular dystrophy (BMD) are genetic neuromuscular disorders caused by mutations in the dystrophin gene, and read-through therapies combined with NMD inhibitors may improve the treatment outcomes for these diseases by increasing the levels of mutant dystrophin mRNA.
HUMAN MOLECULAR GENETICS
(2023)
Article
Immunology
Helene Sertznig, Fabian Roesmann, Alexander Wilhelm, Delia Heininger, Barbara Bleekmann, Carina Elsner, Mario Santiago, Jonas Schuhenn, Zehra Karakoese, Yvonne Benatzy, Ryan Snodgrass, Stefan Esser, Kathrin Sutter, Ulf Dittmer, Marek Widera
Summary: This study shows that HIV-1 infection is associated with low levels of SRSF1 in intestinal and peripheral blood mononuclear cells. The repression of SRSF1 leads to increased viral RNA levels, but reduced levels of the Vif coding transcripts. However, in the presence of high levels of APOBEC3G, the decrease in SRSF1 enhances overall replication.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yuesheng Lv, Wenjing Zhang, Jinyao Zhao, Bing Sun, Yangfan Qi, Haoyu Ji, Chaoqun Chen, Jinrui Zhang, Junxiu Sheng, Taishu Wang, Daniel Dominguez, Han Liu, Quentin Liu, Songshu Meng, Xiaoling Li, Yang Wang
Summary: SRSF1 is identified as an autophagy suppressor that inhibits autophagosome formation by promoting the splicing of Bcl-x and disrupting the Beclin1-PIK3C3 complex. The decrease of SRSF1 stabilizes the Beclin1 and PIK3C3 complex, activating autophagy, while starvation- and oxidative stresses-induced autophagy can degrade SRSF1 through interacting with LC3-II. This positive feedback mechanism is crucial for inhibiting Gefitinib-resistant cancer cell progression.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Andreia E. S. Cunha, Rui J. S. Loureiro, Carlos J. V. Simoes, Rui M. M. Brito
Summary: Influenza viruses cause significant morbidity and mortality through seasonal outbreaks and pandemics. Due to their high rate of evolution, yearly vaccine updates are required and antiviral drugs are less effective. Non-structural protein 1 (NS1) has emerged as a promising target for influenza antiviral therapy, as it plays essential roles in virus replication and pathogenesis. Through molecular dynamics simulations, we identified druggable pockets on NS1 that are partially conserved and co-localize with stable binding regions for PI3K and TRIM25, suggesting their potential as new targets for antiviral development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Yanhui Zhang, Aifang Wang, Xiaohe Zhang, Xiaoliang Wang, Jin Zhang, Jinji Ma
Summary: The highly expressed LINC01296 in oral squamous cell carcinoma promotes tumor progression by binding to SRSF1 protein, demonstrating its role in enhancing proliferation, invasion, and migration of the tumor cells, providing important insights for the prevention, diagnosis, and treatment of oral squamous cell carcinoma.
BIOMED RESEARCH INTERNATIONAL
(2021)
Article
Multidisciplinary Sciences
Waqar Arif, Bhoomika Mathur, Michael F. Saikali, Ullas V. Chembazhi, Katelyn Toohill, You Jin Song, Qinyu Hao, Saman Karimi, Steven M. Blue, Brian A. Yee, Eric L. Van Nostrand, Sushant Bangru, Grace Guzman, Gene W. W. Yeo, Kannanganattu V. Prasanth, Sayeepriyadarshini Anakk, Carolyn L. Cummins, Auinash Kalsotra
Summary: This study reveals that SRSF1 deficiency leads to detrimental R-loop formation and genotoxicity, causing impaired gene expression, metabolism, and lipid trafficking in liver cells, ultimately resulting in NASH pathology. SRSF1 plays a crucial role in maintaining genome stability and tissue homeostasis.
NATURE COMMUNICATIONS
(2023)
Article
Biology
Fiona Haward, Magdalena M. Maslon, Patricia L. Yeyati, Nicolas Bellora, Jan N. Hansen, Stuart Aitken, Jennifer Lawson, Alex von Kriegsheim, Dagmar Wachten, Pleasantine Mill, Ian R. Adams, Javier F. Caceres
Summary: Shuttling RNA-binding proteins play a crucial role in coordinating gene expression between the nucleus and cytoplasm. By creating a mouse model with SRSF1 protein exclusively retained in the nucleus, researchers found that this alteration led to various cellular and tissue defects, particularly in the context of ciliogenesis. This study highlights the importance of SRSF1 shuttling in reprogramming gene expression networks under high cellular demands, such as during motile ciliogenesis.
Article
Biochemistry & Molecular Biology
Andrew M. Jobbins, Nejc Haberman, Natalia Artigas, Christopher Amourda, Helen A. B. Paterson, Sijia Yu, Samuel J. Blackford, Alex Montoya, Marian Dore, Yi-Fang Wang, Alessandro Sardini, Ines Cebola, Johannes Zuber, Sheikh Tamir Rashid, Boris Lenhard, Santiago Vernia
Summary: Pre-mRNA processing is altered in non-alcoholic fatty liver disease (NAFLD), with dysregulated alternative splicing and alternative polyadenylation. Decreased expression of SRSF10, a splicing factor, is associated with dysregulation of pre-mRNA processing in NAFLD.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Plant Sciences
Shanlan Chen, Yujian Mo, Yingjie Zhang, Hongbao Zhu, Yu Ling
Summary: SR proteins from sweet potato have similar functional domains and gene structures as those from Arabidopsis and rice. However, their expression patterns and alternative splicing regulations have changed under stresses. Novel alternative splicing regulations were found in sweet potato SR genes.
Review
Microbiology
Florentin Pastor, Lulzim Shkreta, Benoit Chabot, David Durantel, Anna Salvetti
Summary: Protein phosphorylation is crucial in regulating protein half-life, intracellular distribution, and activity, with CMGC kinases playing a key role in gene expression through splicing control. These kinases, belonging to different families, also influence viral replication, indicating potential translational opportunities.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Plant Sciences
Tongtong Wang, Xiaofeng Wang, Haiyan Wang, Chao Yu, Chengyun Xiao, Yiwu Zhao, Huanan Han, Shuangshuang Zhao, Qun Shao, Jianhua Zhu, Yanxiu Zhao, Pingping Wang, Changle Ma
Summary: Alternative splicing of pre-mRNAs is crucial for plant growth and development. Serine/arginine-rich (SR) proteins are a conserved family of RNA-binding proteins that play a critical role in both constitutive and alternative splicing. However, the regulation of SR proteins' phosphorylation and its effect on gene transcription and alternative splicing during plant development remain poorly understood. In this study, we found that SRPKIIs in Arabidopsis specifically control the phosphorylation status of a subset of SR proteins, including SR45 and SC35, and regulate their subcellular localization.
Review
Biochemistry & Molecular Biology
Jiawei Tang, Youquan Xie, Jingxun Huang, Liao Zhang, Weiye Jiang, Zhiyu Li, Jinlei Bian
Summary: This review discusses the impact of dysregulated alternative splicing on cancer and the role of SR proteins and SR protein-related kinases in cancer development. Small molecule inhibitors targeting SR proteins and SR protein-related kinases are categorized and summarized in terms of structure, inhibition target, mechanism of action, biological activity, and applicable diseases. This review aims to provide a timely summary of recent advances in alternative splicing regulated by kinases and an introduction to relevant small molecule inhibitors.
BIOORGANIC & MEDICINAL CHEMISTRY
(2022)
Article
Virology
Tong Wang, Fanhua Wei, Zhimin Jiang, Jingwei Song, Chengye Li, Jinhua Liu
Summary: The NS1 protein of influenza A viruses was found to enhance virulence by antagonizing host innate anti-viral responses. It was identified that the 14-3-3 epsilon protein interacts with NS1 and prevents the expression of IFN-beta, thereby inhibiting the immune response.
Article
Immunology
Matteo Mombelli, Dionysios Neofytos, Uyen Huynh-Do, Javier Sanchez-Cespedes, Susanne Stampf, Dela Golshayan, Suzan Dahdal, Guido Stirnimann, Aurelia Schnyder, Christian Garzoni, Reto M. Venzin, Lorenzo Magenta, Melanie Schonenberger, Laura Walti, Cedric Hirzel, Aline Munting, Michael Dickenmann, Michael Koller, John-David Aubert, Jurg Steiger, Manuel Pascual, Thomas F. Mueller, Mace Schuurmans, Christoph Berger, Isabelle Binet, Jean Villard, Nicolas J. Mueller, Adrian Egli, Elisa Cordero, Christian van Delden, Oriol Manuel
Summary: The use of MF59-adjuvanted or high-dose influenza vaccine in solid-organ transplant recipients was safe and resulted in a higher vaccine response rate.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Immunology
Marius Troseid, Piotr Nowak, Jessica Nystrom, Annica Lindkvist, Samir Abdurahman, Anders Sonnerborg
Article
Microbiology
Samir Abdurahman, Akos Vegvari, Masoud Youssefi, Michael Levi, Stefan Hoglund, Elin Andersson, Peter Horal, Bo Svennerholm, Jan Balzarini, Anders Vahlne
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2008)
Article
Biotechnology & Applied Microbiology
Babilonia Barqasho, Piotr Nowak, Samir Abdurahman, Lillian Walther-Jallow, Anders Sonnerborg
JOURNAL OF GENERAL VIROLOGY
(2010)
Article
Multidisciplinary Sciences
Jan Vesterbacka, Piotr Nowak, Babilonia Barqasho, Samir Abdurahman, Jessica Nystrom, Staffan Nilsson, Hiroyuki Funaoka, Tatsuo Kanda, Lars-Magnus Andersson, Magnus Gisslen, Anders Sonnerborg
Article
Multidisciplinary Sciences
Halime Ekici, Wondwossen Amogne, Getachew Aderaye, Lars Lindquist, Anders Sonnerborg, Samir Abdurahman
Article
Virology
Samir Abdurahman, Akos Vegvari, Michael Levi, Stefan Hoglund, Marita Hogberg, Weimin Tong, Ivan Romero, Jan Balzarini, Anders Vahlne
Article
Immunology
Samir Abdurahman, Babilonia Barqasho, Piotr Nowak, Do Duy Cuong, Wondwossen Amogne, Mattias Larsson, Lars Lindquist, Gaetano Marrone, Anders Sonnerborg
JOURNAL OF THE INTERNATIONAL AIDS SOCIETY
(2014)
Article
Virology
Samir Abdurahman, Masoud Youssefi, Stefan Hoglund, Anders Vahlne
Article
Virology
Samir Abdurahman, Stefan Hoglund, Anders Hoglund, Anders Vahlne
Article
Biotechnology & Applied Microbiology
S Abdurahman, S Höglund, L Goobar-Larsson, A Vahlne
JOURNAL OF GENERAL VIROLOGY
(2004)
Article
Virology
Naiqing Xu, Xinen Tang, Xin Wang, Miao Cai, Xiaowen Liu, Xiaolong Lu, Shunlin Hu, Min Gu, Jiao Hu, Ruyi Gao, Kaituo Liu, Yu Chen, Xiufan Liu, Xiaoquan Wang
Summary: This study found that the H9N2 subtype avian influenza virus has a high airborne transmissibility, while the H7N9 virus does not. The Hemagglutinin protein of the H9N2 virus was found to play a key role in replication, stability, and airborne transmission.
Article
Virology
Samar S. Ewies, Sabry M. Tamam, Ahmed S. Abdel-Moneim, Sherin R. Rouby
Summary: Contagious ecthyma (CE) is a highly contagious viral disease of sheep and goats worldwide. The study provided a clinical description of CE and screened for genetic variation in the B2L gene. Infected sheep exhibited anorexia and oral lesions, while inoculated chicken embryos showed pock lesions. The B2L gene was successfully amplified and found to be highly conserved.
Article
Virology
Yigal Farnoushi, Dan Heller, Avishai Lublin
Summary: In recent years, new variants of avian reovirus (ARV) have caused a variety of symptoms in chickens worldwide, including viral arthritis/tenosynovitis. This study analyzed emerging ARV variants in Israel and found significant genetic diversity. Most ARV isolates in Israel belonged to genotypic cluster 5 (GC5). The study suggests that Israel has not experienced the emergence of new ARV variants since the introduction of the live vaccine (ISR-7585), but ongoing monitoring is needed due to the continuous emergence of ARV variants.
Article
Virology
Shigeru Tajima, Michiyo Kataoka, Yuki Takamatsu, Hideki Ebihara, Chang-Kweng Lim
Summary: Yokose virus (YOKV), a bat-associated flavivirus, was found to replicate at a slower rate in mosquito cells compared to other mosquito-borne flaviviruses. Specific nucleotide mutations in the virus were identified to enhance its proliferation ability in mosquito cells.
Article
Virology
Alejandra Borjabad, Baojun Dong, Wei Chao, David J. Volsky, Mary Jane Potash
Summary: This study investigated HIV brain disease using a mouse model, and found that poly I:C can reverse associated cognitive impairment and reduce virus burden. The results also revealed transcriptional changes related to neuronal function and innate immune responses.
Article
Virology
Ching-Hung Lin, Feng-Cheng Hsieh, Meilin Wang, Chieh Hsu, Hsuan-Wei Hsu, Chun-Chun Yang, Cheng-Yao Yang, Hung-Yi Wu
Summary: This study demonstrates that the synthesis of coronavirus subgenomic mRNA is not solely determined by the sequence homology between the leader TRS and TRS-B, but also by the disassociation of the coronavirus polymerase from the viral genome. This finding provides a new insight into the transcription mechanism of coronaviruses.
Article
Virology
Nicholas S. Kron, Benjamin W. Neuman, Sathish Kumar, Patricia L. Blackwelder, Dayana Vidal, Delphina Z. Walker-Phelan, Patrick D. I. Gibbs, Lynne A. Fieber, Michael C. Schmale
Summary: Two recent studies documented the genome of a novel virus in marine animals, finding that the virus is widespread in apparently healthy animals but not highly expressed in neurons. The studies also identified viral replication factories and high levels of defective genomes in chronically infected animals.
Article
Virology
Andrew M. Ramey, Laura C. Scott, Christina A. Ahlstrom, Evan J. Buck, Alison R. Williams, Mia Kim Torchetti, David E. Stallknecht, Rebecca L. Poulson
Summary: We successfully detected and characterized highly pathogenic avian influenza viruses in hunter-harvested wild waterfowl samples from western Alaska. Genomic analysis revealed three independent viral introductions into Alaska. Our findings demonstrate the utility and potential limitations of using molecular processing approaches directly on original swab samples for viral research and monitoring.
Article
Virology
Ting Gong, Dongdong Wu, Yongzhi Feng, Xing Liu, Qi Gao, Xiaoyu Zheng, Zebu Song, Heng Wang, Guihong Zhang, Lang Gong
Summary: This study discovered that quercetin can inhibit PEDV replication both in vivo and in vitro, and alleviate the clinical symptoms and intestinal injury caused by the virus. This provides a new direction for the development of PED antiviral drugs.
Article
Virology
Min Zhu, Hao Zeng, Jianqiao He, Yaohui Zhu, Pingping Wang, Jianing Guo, Jinfan Guo, Huabo Zhou, Yifeng Qin, Kang Ouyang, Zuzhang Wei, Weijian Huang, Ying Chen
Summary: The reassortment between avian H9N2 and Eurasian avian-like (EA) H1N1 viruses may have potentially changed from avian-to-mammals adaptation. This study found that the introduction of EA H1N1 internal genes into H9N2 virus restored the replication capability and resulted in extreme virulence in some cases. This raises new concerns for public health due to the possible coexistence of H9N2 and EA H1N1 viruses in dogs.