期刊
VIROLOGY
卷 401, 期 2, 页码 215-227出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2010.02.021
关键词
Feline herpesvirus; FHV-1; Genomic sequence; Infectious BAC clone
类别
资金
- Companion Animal Fund
- Center for Feline Health and Well-Being at Michigan State University's College of Veterinary Medicine
Infection with feline herpesvirus-1 (FHV-1) is a major cause of upper respiratory and ocular diseases in Felidae. We report the first complete genomic sequence of FHV-1, as well as the construction and characterization of a bacterial artificial chromosome (BAC) clone of FHV-1, which contains the entire FHV-1 genome and has the BAC vector inserted at the left end of U-L. Complete genomic sequences were derived from both the FHV-1 BAC clone and purified virion DNA. The FHV-1 genome is 135,797 bp in size with an overall G + C content of 45%. A total of 78 open reading frames were predicted, encoding 74 distinct proteins. The gene arrangement is collinear with that of most sequenced varicelloviruses. The virus regenerated from the BAC was very similar to the parental C-27 strain in vitro in terms of plaque morphology and growth characteristics and highly virulent in cats in a preliminary in vivo study. (C) 2010 Elsevier Inc. All rights reserved.
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