Article
Biochemistry & Molecular Biology
Yu-Shin Sou, Junji Yamaguchi, Hiroshi Kameda, Keisuke Masuda, Yusuke Maeda, Yasuo Uchiyama, Masato Koike
Summary: GPHR is important for maintaining proper cholesterol levels and neuronal morphology by regulating the acidic pH of the Golgi apparatus. Knockout of GPHR leads to abnormal Golgi morphology and neurodegeneration.
Review
Cell Biology
Xiaoyan Zhang
Summary: The Golgi apparatus serves as a major processing station in the cell and requires a well-organized structure for proper protein glycosylation. Altered glycosylation is a hallmark of most cancer cells, and Golgi structural proteins are associated with tumor progression. Understanding Golgi structural and functional defects may help determine whether glycosylation defect is a cause or effect of oncogenesis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Virology
Antonio Real-Hohn, Martin Groznica, Georg Kontaxis, Rong Zhu, Otavio Augusto Chaves, Leonardo Vazquez, Peter Hinterdorfer, Heinrich Kowalski, Dieter Blaas
Summary: Rhinoviruses are the main cause of the common cold and can lead to complications in patients with other ailments. Colds have a significant socioeconomic impact, but there are no vaccines or approved treatments available.
Article
Virology
Seyedehmahsa Moghimi, Ekaterina Viktorova, Anna Zimina, Tomasz Szul, Elizabeth Sztul, George A. Belov
Summary: The research revealed the diverse roles of different Arfs during enterovirus infection, with Arf1 playing a crucial role in replication. Additionally, Arf6, known for its association with the plasma membrane, was found to be important for viral replication. This discovery may offer new insights into controlling enterovirus infections.
JOURNAL OF VIROLOGY
(2021)
Article
Virology
Danielle M. M. Pierce, Connor Hayward, David J. J. Rowlands, Nicola J. J. Stonehouse, Morgan R. R. Herod
Summary: FMDV is the causative agent of FMD, a significant disease in farmed animals that can result in major economic losses. The replication of this virus involves a highly coordinated processing of nonstructural proteins within membrane-associated compartments. Mutations at the 3B(3)-3C boundary increase the rate of proteolysis and affect the production of enzymatic nonstructural proteins.
JOURNAL OF VIROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Hsiang-Yi Chang, Wei Yuan Yang
Summary: Organelles can be disrupted by various factors, and autophagy is an important response for eliminating compromised organelles. The Golgi apparatus can be targeted for removal through autophagy, but certain components can also be eliminated through autophagosome-independent pathways when the Golgi is stressed. Future studies will investigate the coordination between different mechanisms in driving Golgi degradation and quantify the contribution of autophagy to Golgi maintenance.
Review
Cell Biology
Jiamin Yi, Jiangling Peng, Wenping Yang, Guoqiang Zhu, Jingjing Ren, Dan Li, Haixue Zheng
Summary: Protease 3C, encoded by all known picornaviruses, plays crucial roles in viral replication by cleaving viral polyproteins and assembling the viral RNA replication complex. It also interacts with host proteins to promote viral replication and inhibit host immune responses, ultimately contributing to picornavirus-mediated pathogenesis.
JOURNAL OF CELL SCIENCE
(2021)
Article
Multidisciplinary Sciences
Daniel L. Hurdiss, Priscila El Kazzi, Lisa Bauer, Nicolas Papageorgiou, Francois P. Ferron, Tim Donselaar, Arno L. W. van Vliet, Tatiana M. Shamorkina, Joost Snijder, Bruno Canard, Etienne Decroly, Andrea Brancale, Tzviya Zeev-Ben-Mordehai, Friedrich Forster, Frank J. M. van Kuppeveld, Bruno Coutard
Summary: This study reveals the crystal structure of the binding complex between enterovirus 2C protein and fluoxetine, uncovering an allosteric binding site. By engineering a hexameric form of 2C protein, the study demonstrates that compounds like fluoxetine can inhibit the ATPase activity of 2C. Cryo-electron microscopy analysis further shows how fluoxetine locks 2C protein in a hexameric state. These findings provide important insights into the inhibition mechanism of 2C and offer a robust engineering strategy for studying its structure, function, and drug-screening analysis.
Article
Oncology
Artem N. Pachikov, Ryan R. Gough, Caroline E. Christy, Mary E. Morris, Carol A. Casey, Chad A. LaGrange, Ganapati Bhat, Anatoly Kubyshkin, Iryna I. Fomochkina, Evgeniya Y. Zyablitskaya, Tatiana P. Makalish, Elena P. Golubinskaya, Kateryna A. Davydenko, Sergey N. Eremenko, Jean-Jack M. Riethoven, Amith S. Maroli, Thomas S. Payne, Robert Powers, Alexander Y. Lushnikov, Amanda J. Macke, Armen Petrosyan
Summary: The progression of prostate cancer (PCa) is associated with the translocation of S1P and S2P proteases to the endoplasmic reticulum (ER) and subsequent cleavage of ATF6, leading to enhanced unfolded protein response (UPR) and cell proliferation. By utilizing various ER stress models, it was discovered that PCa cells employ an elegant mechanism to auto-activate ER stress and promote cell proliferation.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2021)
Article
Virology
Nana Wang, Haiwei Wang, Jiabao Shi, Chen Li, Xinran Liu, Junhao Fan, Chao Sun, Craig E. Cameron, Hong Qi, Li Yu
Summary: Senecavirus A (SVA) is a picornavirus that causes vesicular disease in swine and is the only member of its genus. The IRES in SVA plays a crucial role in cap-independent translation. Studies have shown that the stem-loop I element in SVA's IRES is essential for IRES activity and viral replication.
Article
Microbiology
Manel Essaidi-Laziosi, Lena Royston, Bernadett Boda, Francisco Javier Perez-Rodriguez, Isabelle Piuz, Nicolas Hulo, Laurent Kaiser, Sophie Clement, Song Huang, Samuel Constant, Caroline Tapparel
Summary: By comparing infections of in vitro reconstituted airway epithelia from asthmatic and healthy donors, we found that tissues from asthmatic donors were more susceptible to respiratory viruses and had higher viral replication levels. In addition, viral infections led to alterations in gene expression, affecting cilia structure and motility, mucociliary clearance, and mucus secretion. These findings shed light on the mechanisms of asthma exacerbations and provide potential therapeutic targets.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Christine E. Peters, Ursula Schulze-Gahmen, Manon Eckhardt, Gwendolyn M. Jang, Jiewei Xu, Ernst H. Pulido, Melanie Ott, Or Gozani, Kliment A. Verba, Ruth Huettenhain, Jan E. Carette, Nevan J. Krogan
Summary: This study reports the structure of the interaction between Actin histidine methyltransferase SETD3 and the enterovirus CV-B3 2A protease, revealing the importance of the SET domain for virus replication. Mutations in key residues of the SET domain result in reduced binding to 2A and complete protection against enteroviral infection.
NATURE COMMUNICATIONS
(2022)
Article
Neurosciences
Henriette Haukedal, Giulia I. Corsi, Veerendra P. Gadekar, Nadezhda T. Doncheva, Shekhar Kedia, Noortje de Haan, Abinaya Chandrasekaran, Pia Jensen, Pernille Schionning, Sarah Vallin, Frederik Ravnkilde Marlet, Anna Poon, Carlota Pires, Fawzi Khoder Agha, Hans H. Wandall, Susanna Cirera, Anja Hviid Simonsen, Troels Tolstrup Nielsen, Jorgen Erik Nielsen, Poul Hyttel, Ravi Muddashetty, Blanca I. Aldana, Jan Gorodkin, Deepak Nair, Morten Meyer, Martin Rossel Larsen, Kristine Freude
Summary: Alzheimer's disease is a common cause of dementia with no cure. This study investigated specific disease phenotypes in glutamatergic forebrain neurons and identified Golgi fragmentation as one of the earliest AD phenotypes. The study also found that genetic variants in SORL1 could aggravate Golgi fragmentation and glycosylation changes.
FRONTIERS IN NEUROSCIENCE
(2023)
Editorial Material
Microbiology
David Aponte-Diaz, Matthew R. Vogt, Craig E. Cameron
Summary: EV-D68 contributes significantly to respiratory and neurological diseases. It exploits cellular autophagic compartments and its viral proteins 2B and 3A have difficulty engaging membranes, leading to a delay in RNA synthesis.
Article
Virology
Alexis Bouin, Michelle N. Vu, Ali Al-Hakeem, Genevieve P. Tran, Joseph H. C. Nguyen, Bert L. Semler
Summary: Group B enteroviruses, including coxsackievirus B3 (CVB3), can persistently infect cardiac tissue and cause dilated cardiomyopathy. 5' terminal deletions of viral genomic RNAs have been detected in persistently infected patients, and our study showed that these deletions decreased the levels of viral proteinase activity and synthesis of viral protein and RNA. However, a cooperative interaction was observed when full-length and terminally deleted forms were cotransfected into cardiomyocytes, leading to increased viral production.
JOURNAL OF VIROLOGY
(2023)
Article
Public, Environmental & Occupational Health
Claire A. Quiner, Yoshinori Nakazawa
INTERNATIONAL JOURNAL OF HEALTH GEOGRAPHICS
(2017)
Article
Virology
Claire A. Quiner, Poornima Parameswaran, Alexander T. Ciota, Dylan J. Ehrbar, Brittany L. Dodson, Sondra Schlesinger, Laura D. Kramer, Eva Harris
JOURNAL OF VIROLOGY
(2014)
Article
Multidisciplinary Sciences
Claire A. Quiner, Cynthia Moses, Benjamin P. Monroe, Yoshinori Nakazawa, Jeffrey B. Doty, Christine M. Hughes, Andrea M. McCollum, Saturnin Ibata, Jean Malekani, Emile Okitolonda, Darin S. Carroll, Mary G. Reynolds
Article
Hematology
Eduard Grebe, Michael P. Busch, Edward P. Notari, Roberta Bruhn, Claire Quiner, Daniel Hindes, Mars Stone, Sonia Bakkour, Hong Yang, Phillip Williamson, Debra Kessler, Rita Reik, Susan L. Stramer, Simone A. Glynn, Steven A. Anderson, Alan E. Williams, Brian Custer
Article
Hematology
Brian Custer, Claire Quiner, Richard Haaland, Amy Martin, Mars Stone, Rita Reik, Whitney R. Steele, Debra Kessler, Phillip C. Williamson, Steven A. Anderson, Alan E. Williams, Henry F. Raymond, Willi McFarland, William T. Robinson, Sara Glick, Kwa Sey, C. David Melton, Simone A. Glynn, Susan L. Stramer, Michael P. Busch
Article
Hematology
Claire Quiner, Roberta Bruhn, Eduard Grebe, Clara Di Germanio, Debra Kessler, Rita Reik, Phillip Williamson, Dylan Hampton, Rahima Fayed, Steve A. Anderson, Alan E. Williams, Simone A. Glynn, Michael P. Busch, Susan L. Stramer, Brian Custer
Article
Mathematical & Computational Biology
Claire Quiner, Kasey Jones, Georgiy Bobashev
Summary: This study examines the impact of revocable behavioral interventions, such as shelter-in-place, on epidemics and emphasizes the role of the proportion of susceptible individuals. The timing, duration, and intensity of interventions have significant effects on disease transmission. Through modeling the COVID-19 dynamics in Wake County, North Carolina, the researchers found that early interventions can shift the epidemic curve, while interventions near the peak can modify its shape and case count. Accurate estimation of the proportion susceptible is crucial for predicting the impact of interventions. The findings highlight the importance of considering susceptibility in intervention design and decision-making.
INFECTIOUS DISEASE MODELLING
(2022)
Meeting Abstract
Hematology
Brian Custer, Claire Quiner, Rich Haaland, Amy Martin, Mars Stone, Rita A. Reik, Whitney R. Steele, Debra A. Kessler, Phillip C. Williamson, Steven A. Anderson, Alan Williams, Susan L. Stramer, Michael P. Busch
Meeting Abstract
Hematology
Brian Custer, Eda Altan, Roberta Bruhn, Claire Quiner, Eric Delwart, Dylan Hampton, Sonia Bakkour, Mars Stone, Rita A. Reik, Whitney Steele, Debra A. Kessler, Phillip C. Williamson, Steven Anderson, Susan L. Stramer, Michael P. Busch
Meeting Abstract
Hematology
Roberta Bruhn, Eda Altan, Claire Quiner, Eric Delwart, Sonia Bakkour, Whitney Steele, Ed P. Notari, Rahima Fayed, Valerie Winkelman, Mars Stone, Rita A. Reik, Debra A. Kessler, Phillip C. Williamson, Steven Anderson, Susan L. Stramer, Michael P. Busch, Brian Custer
Article
Virology
Naiqing Xu, Xinen Tang, Xin Wang, Miao Cai, Xiaowen Liu, Xiaolong Lu, Shunlin Hu, Min Gu, Jiao Hu, Ruyi Gao, Kaituo Liu, Yu Chen, Xiufan Liu, Xiaoquan Wang
Summary: This study found that the H9N2 subtype avian influenza virus has a high airborne transmissibility, while the H7N9 virus does not. The Hemagglutinin protein of the H9N2 virus was found to play a key role in replication, stability, and airborne transmission.
Article
Virology
Samar S. Ewies, Sabry M. Tamam, Ahmed S. Abdel-Moneim, Sherin R. Rouby
Summary: Contagious ecthyma (CE) is a highly contagious viral disease of sheep and goats worldwide. The study provided a clinical description of CE and screened for genetic variation in the B2L gene. Infected sheep exhibited anorexia and oral lesions, while inoculated chicken embryos showed pock lesions. The B2L gene was successfully amplified and found to be highly conserved.
Article
Virology
Yigal Farnoushi, Dan Heller, Avishai Lublin
Summary: In recent years, new variants of avian reovirus (ARV) have caused a variety of symptoms in chickens worldwide, including viral arthritis/tenosynovitis. This study analyzed emerging ARV variants in Israel and found significant genetic diversity. Most ARV isolates in Israel belonged to genotypic cluster 5 (GC5). The study suggests that Israel has not experienced the emergence of new ARV variants since the introduction of the live vaccine (ISR-7585), but ongoing monitoring is needed due to the continuous emergence of ARV variants.
Article
Virology
Shigeru Tajima, Michiyo Kataoka, Yuki Takamatsu, Hideki Ebihara, Chang-Kweng Lim
Summary: Yokose virus (YOKV), a bat-associated flavivirus, was found to replicate at a slower rate in mosquito cells compared to other mosquito-borne flaviviruses. Specific nucleotide mutations in the virus were identified to enhance its proliferation ability in mosquito cells.
Article
Virology
Alejandra Borjabad, Baojun Dong, Wei Chao, David J. Volsky, Mary Jane Potash
Summary: This study investigated HIV brain disease using a mouse model, and found that poly I:C can reverse associated cognitive impairment and reduce virus burden. The results also revealed transcriptional changes related to neuronal function and innate immune responses.
Article
Virology
Ching-Hung Lin, Feng-Cheng Hsieh, Meilin Wang, Chieh Hsu, Hsuan-Wei Hsu, Chun-Chun Yang, Cheng-Yao Yang, Hung-Yi Wu
Summary: This study demonstrates that the synthesis of coronavirus subgenomic mRNA is not solely determined by the sequence homology between the leader TRS and TRS-B, but also by the disassociation of the coronavirus polymerase from the viral genome. This finding provides a new insight into the transcription mechanism of coronaviruses.
Article
Virology
Nicholas S. Kron, Benjamin W. Neuman, Sathish Kumar, Patricia L. Blackwelder, Dayana Vidal, Delphina Z. Walker-Phelan, Patrick D. I. Gibbs, Lynne A. Fieber, Michael C. Schmale
Summary: Two recent studies documented the genome of a novel virus in marine animals, finding that the virus is widespread in apparently healthy animals but not highly expressed in neurons. The studies also identified viral replication factories and high levels of defective genomes in chronically infected animals.
Article
Virology
Andrew M. Ramey, Laura C. Scott, Christina A. Ahlstrom, Evan J. Buck, Alison R. Williams, Mia Kim Torchetti, David E. Stallknecht, Rebecca L. Poulson
Summary: We successfully detected and characterized highly pathogenic avian influenza viruses in hunter-harvested wild waterfowl samples from western Alaska. Genomic analysis revealed three independent viral introductions into Alaska. Our findings demonstrate the utility and potential limitations of using molecular processing approaches directly on original swab samples for viral research and monitoring.
Article
Virology
Ting Gong, Dongdong Wu, Yongzhi Feng, Xing Liu, Qi Gao, Xiaoyu Zheng, Zebu Song, Heng Wang, Guihong Zhang, Lang Gong
Summary: This study discovered that quercetin can inhibit PEDV replication both in vivo and in vitro, and alleviate the clinical symptoms and intestinal injury caused by the virus. This provides a new direction for the development of PED antiviral drugs.
Article
Virology
Min Zhu, Hao Zeng, Jianqiao He, Yaohui Zhu, Pingping Wang, Jianing Guo, Jinfan Guo, Huabo Zhou, Yifeng Qin, Kang Ouyang, Zuzhang Wei, Weijian Huang, Ying Chen
Summary: The reassortment between avian H9N2 and Eurasian avian-like (EA) H1N1 viruses may have potentially changed from avian-to-mammals adaptation. This study found that the introduction of EA H1N1 internal genes into H9N2 virus restored the replication capability and resulted in extreme virulence in some cases. This raises new concerns for public health due to the possible coexistence of H9N2 and EA H1N1 viruses in dogs.
