Article
Virology
Michelle Felicia Lee, Mohd Ishtiaq Anasir, Chit Laa Poh
Summary: Dengue infections pose a critical threat to public health worldwide, and there is an urgent need to develop effective antivirals. Peptides designed to target the DENV envelope protein show promise as antiviral candidates. Among the twelve peptides designed, four exhibited potent inhibitory effects against multiple DENV serotypes. Peptide 3 demonstrated the best overall antiviral activity, while peptide 5F showed particular efficacy against DENV-4 during post-infection treatment.
Article
Entomology
Cinthya Amaral, Daniel Camara, Tiago Salles, Marcelo Damiao Meneses, Carlla De Araujo-Silva, Vanessa Dias, Fabio da Costa, Lucio Caldas, Renata Azevedo
Summary: The Flavivirus genus consists of a wide range of species that infect both vertebrates and invertebrates. Culex Flavivirus (CxFV) is an insect-specific virus that has garnered interest due to its ability to exclude other medically important viruses in nature. However, little is known about the ecology of CxFV and its co-circulation with other arboviruses. This study aimed to observe CxFV infection in mosquitoes captured in an urban area of Rio de Janeiro, Brazil, characterize the virus circulation, and provide isolates. The results showed that the CxFV isolates from this study belonged to genotype 2, which is consistent with sequences from Latin America and Africa.
Article
Immunology
Yuan Fang, Ernest Tambo, Jing-Bo Xue, Yi Zhang, Xiao-Nong Zhou, Emad I. M. Khater
Summary: This study reports the first detection of four arboviruses (DENV, CFAV, PAFV, and CxFV) in mosquitoes in Saudi Arabia, showing their co-circulation in Jeddah. Phylogenetic analysis revealed a close relationship between the detected DENV-2 strain and strains found in China and Japan, indicating frequent movement of DENV-2 strains among these countries, with at least five introductions into Saudi Arabia from India from 2014 to 2018.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Review
Pharmacology & Pharmacy
Ivo C. C. Martins, Rafaela C. C. Ricardo, Nuno C. C. Santos
Summary: Dengue, West Nile, and Zika viruses are vector-borne flaviviruses that cause disease outbreaks with potentially severe symptoms and socioeconomic repercussions. Currently, there are no specific treatments available, and only generic symptom relief is possible. The effectiveness of the first dengue vaccine, Dengvaxia, is limited. Prophylactic approaches against other viruses are even more restricted. Therefore, there is a need for therapeutic strategies.
Article
Microbiology
Mattia Calzolari, Paolo Bonilauri, Annalisa Grisendi, Gastone Dalmonte, Alice Vismarra, Davide Lelli, Chiara Chiapponi, Romeo Bellini, Antonio Lavazza, Michele Dottori
Summary: In this study, we detected several arboviruses, including West Nile virus, Usutu virus, and Tahyna virus, in mosquitoes collected in Emilia-Romagna, Italy in 2021. Our findings suggest the presence of viral hot spots and highlight the importance of investigating Tahyna virus as a potential cause of human diseases.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Guntur Fibriansah, Elisa X. Y. Lim, Jan K. Marzinek, Thiam-Seng Ng, Joanne L. Tan, Roland G. Huber, Xin-Ni Lim, Valerie S. Y. Chew, Victor A. Kostyuchenko, Jian Shi, Ganesh S. Anand, Peter J. Bond, James E. Crowe, Shee-Mei Lok
Summary: Dengue virus has four serotypes (DENV1-4) with different strains within each serotype. The virus can exhibit smooth or bumpy surface morphologies at 37 degrees C, depending on the strain. High-affinity therapeutic antibodies are able to neutralize diverse morphologies of dengue virus.
Article
Immunology
Hong-Jyun Huang, Martyr Yang, Hsin-Wei Chen, Shuying Wang, Chih-Peng Chang, Tzong-Shiann Ho, Yu-San Kao, Sen-Mao Tien, Hsing-Han Lin, Po-Chun Chang, Yen-Chung Lai, Yu-Peng Hsiao, Yi-Ling Liu, Chiao-Hsuan Chao, Robert Anderson, Trai-Ming Yeh, Yee-Shin Lin, Shu-Wen Wan
Summary: This study developed a modified DENV vaccine candidate with enhanced features by combining modifications of two DENV proteins. The vaccine candidate showed protective effects against DENV infection in a mouse model, indicating its potential clinical application.
Article
Microbiology
Seydou Ka, Natacha Merindol, Aissatou Aicha Sow, Amita Singh, Karima Landelouci, Melodie B. Plourde, Genevieve Pepin, Marco Masi, Roberta Di Lecce, Antonio Evidente, Matar Seck, Lionel Berthoux, Laurent Chatel-Chaix, Isabel Desgagne-Penix
Summary: The study found that Amaryllidaceae alkaloids (AAs) from plants of the Amaryllidaceae family have strong antiviral activity, with cherylline specifically inhibiting dengue virus and Zika virus replication. This suggests that AAs, including cherylline, could be a potential source of new antiviral drugs.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Pharmacology & Pharmacy
Yunzheng Yan, Jingjing Yang, Dian Xiao, Jiye Yin, Mengwen Song, Yijie Xu, Lei Zhao, Qingsong Dai, Yuexiang Li, Cui Wang, Zhuang Wang, Xiaofeng Ren, Xiaotong Yang, Jie Ni, Miaomiao Liu, Xiaojia Guo, Wei Li, Xingjuan Chen, Zhiqiang Liu, Ruiyuan Cao, Wu Zhong
Summary: This study found that Nafamostat (NM) has the potential to inhibit flavivirus infections. Through in vitro and in vivo experiments, researchers found that NM effectively inhibits the replication of Zika virus and other flaviviruses. Further research revealed the antiviral mechanism of NM and proposed a novel drug design approach targeting the flavivirus envelope protein.
ANTIVIRAL RESEARCH
(2022)
Article
Immunology
Te-Sheng Lien, Der-Shan Sun, Shih-Che Hung, Wen-Sheng Wu, Hsin-Hou Chang
Summary: The study shows that the dengue virus envelope protein domain III can induce neutrophil NETosis processes, and inhibition of Nlrp3 inflammasome can suppress this process, providing a new molecular target for treating dengue-induced inflammatory pathogenesis.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Xiao Ma, Zhenghong Yuan, Zhigang Yi
Summary: This study revealed the key residues of the E protein involved in the entry and egress of Zika virus. The results showed that DIII-mediated E trimerization is essential for viral egress, while key residues in DII participate in virus entry.
EMERGING MICROBES & INFECTIONS
(2022)
Article
Microbiology
Tao Hu, Zhen Wu, Shaoxiong Wu, Mingshu Wang, Renyong Jia, Dekang Zhu, Mafeng Liu, Xinxin Zhao, Qiao Yang, Ying Wu, Shaqiu Zhang, Juan Huang, Sai Mao, Xumin Ou, Qun Gao, Di Sun, Yunya Liu, Ling Zhang, YanLing Yu, Shun Chen, Anchun Cheng
Summary: The loop regions of the TMUV E domain III play a critical role in virus entry and assembly process, with mutations affecting viral proliferation and infectivity. Specifically, mutations in loops II-IV disrupted virion assembly while overall, loops I-IV were found to be important for virus binding and entry.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Cell Biology
Sundy N. Y. Yang, Belinda Maher, Chunxiao Wang, Kylie M. Wagstaff, Johanna E. Fraser, David A. Jans
Summary: This study identifies two drugs that can inhibit the replication of dengue virus and other flaviviruses by directly binding to a viral protein.
Review
Immunology
Zhuanzhuan Liu, Qingxin Zhang, Liya Li, Junjie He, Jinyang Guo, Zichen Wang, Yige Huang, Zimeng Xi, Fei Yuan, Yiji Li, Tingting Li
Summary: This review explores the correlations between temperature and dengue epidemics, including the distribution and development of Aedes mosquitoes, the structure of DENV, and the vector competence of Aedes mosquitoes. Additionally, it discusses the immune and metabolic mechanism through which temperature affects the vector competence of Aedes mosquitoes to transmit DENV.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Te-Sheng Lien, Der-Shan Sun, Wen-Sheng Wu, Hsin-Hou Chang
Summary: This study aimed to investigate the cytotoxicity of EIII-coated silica nanoparticles (EIII-SNPs) on endothelial cells and their potential role in hemorrhage pathogenesis. The results showed that EIII-SNPs induced greater endothelial cytotoxicity in vitro than EIII or silica nanoparticles alone. Moreover, the combination of EIII-SNPs and antiplatelet antibodies resulted in more severe hemorrhage pathogenesis in mice compared to single treatments. These findings suggest that EIII-coated nanoparticles could be used to develop a dengue hemorrhage pathogenesis model and highlight the potential role of EIII in DHF pathogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Public, Environmental & Occupational Health
Kristen Marshall, Grace M. Vahey, Emily McDonald, Jacqueline E. Tate, Rachel Herlihy, Claire M. Midgley, Breanna Kawasaki, Marie E. Killerby, Nisha B. Alden, J. Erin Staples
MMWR-MORBIDITY AND MORTALITY WEEKLY REPORT
(2020)
Article
Virology
Jens H. Kuhn, Scott Adkins, Daniela Alioto, Sergey V. Alkhovsky, Gaya K. Amarasinghe, Simon J. Anthony, Tatjana Avsic-Zupanc, Maria A. Ayllon, Justin Bahl, Anne Balkema-Buschmann, Matthew J. Ballinger, Tomas Bartonicka, Christopher Basler, Sina Bavari, Martin Beer, Dennis A. Bente, Eric Bergeron, Brian H. Bird, Carol Blair, Kim R. Blasdell, Steven B. Bradfute, Rachel Breyta, Thomas Briese, Paul A. Brown, Ursula J. Buchholz, Michael J. Buchmeier, Alexander Bukreyev, Felicity Burt, Nihal Buzkan, Charles H. Calisher, Mengji Cao, Inmaculada Casas, John Chamberlain, Kartik Chandran, Remi N. Charrel, Biao Chen, Michela Chiumenti, Il-Ryong Choi, J. Christopher S. Clegg, Ian Crozier, John V. da Graca, Elena Dal Bo, Alberto M. R. Davila, Juan Carlos de la Torre, Xavier de Lamballerie, Rik L. de Swart, Patrick L. Di Bello, Nicholas Di Paola, Francesco Di Serio, Ralf G. Dietzgen, Michele Digiaro, Valerian V. Dolja, Olga Dolnik, Michael A. Drebot, Jan Felix Drexler, Ralf Duerrwald, Lucie Dufkova, William G. Dundon, W. Paul Duprex, John M. Dye, Andrew J. Easton, Hideki Ebihara, Toufic Elbeaino, Koray Ergunay, Jorlan Fernandes, Anthony R. Fooks, Pierre B. H. Formenty, Leonie F. Forth, Ron A. M. Fouchier, Juliana Freitas-Astua, Selma Gago-Zachert, George Fu Gao, Maria Laura Garcia, Adolfo Garcia-Sastre, Aura R. Garrison, Aiah Gbakima, Tracey Goldstein, Jean-Paul J. Gonzalez, Anthony Griffiths, Martin H. Groschup, Stephan Guenther, Alexandro Guterres, Roy A. Hall, John Hammond, Mohamed Hassan, Jussi Hepojoki, Satu Hepojoki, Udo Hetzel, Roger Hewson, Bernd Hoffmann, Seiji Hongo, Dirk Hoeper, Masayuki Horie, Holly R. Hughes, Timothy H. Hyndman, Amara Jambai, Rodrigo Jardim, Daohong Jiang, Qi Jin, Gilda B. Jonson, Sandra Junglen, Serpil Karadag, Karen E. Keller, Boris Klempa, Jonas Klingstrom, Gary Kobinger, Hideki Kondo, Eugene V. Koonin, Mart Krupovic, Gael Kurath, Ivan V. Kuzmin, Lies Laenen, Robert A. Lamb, Amy J. Lambert, Stanley L. Langevin, Benhur Lee, Elba R. S. Lemos, Eric M. Leroy, Dexin Li, Jianrong Li, Mifang Liang, Wenwen Liu, Yan Liu, Igor S. Lukashevich, Piet Maes, William Marciel de Souza, Marco Marklewitz, Sergio H. Marshall, Giovanni P. Martelli, Robert R. Martin, Shin-Yi L. Marzano, Sebastien Massart, John W. McCauley, Nicole Mielke-Ehret, Angelantonio Minafra, Maria Minutolo, Ali Mirazimi, Hans-Peter Muehlbach, Elke Muhlberger, Rayapati Naidu, Tomohide Natsuaki, Beatriz Navarro, Jose A. Navarro, Sergey V. Netesov, Gabriele Neumann, Norbert Nowotny, Marcio R. T. Nunes, Are Nylund, Arnfinn L. Okland, Renata C. Oliveira, Gustavo Palacios, Vicente Pallas, Bernadett Palyi, Anna Papa, Colin R. Parrish, Alex Pauvolid-Correa, Janusz T. Paweska, Susan Payne, Daniel R. Perez, Florian Pfaff, Sheli R. Radoshitzky, Aziz-ul Rahman, Pedro L. Ramos-Gonzalez, Renato O. Resende, Carina A. Reyes, Bertus K. Rima, Victor Romanowski, Gabriel Robles Luna, Paul Rota, Dennis Rubbenstroth, Jonathan A. Runstadler, Daniel Ruzek, Sead Sabanadzovic, Jiri Salat, Amadou Alpha Sall, Maria S. Salvato, Kamil Sarpkaya, Takahide Sasaya, Martin Schwemmle, Muhammad Z. Shabbir, Xiaohong Shi, Zhengli Shi, Yukio Shirako, Peter Simmonds, Jana Sirmarova, Manuela Sironi, Sophie Smither, Teemu Smura, Jin-Won Song, Kirsten M. Spann, Jessica R. Spengler, Mark D. Stenglein, David M. Stone, Petra Strakova, Ayato Takada, Robert B. Tesh, Natalie J. Thornburg, Keizo Tomonaga, Noel Tordo, Jonathan S. Towner, Massimo Turina, Ioannis Tzanetakis, Rainer G. Ulrich, Anna Maria Vaira, Bernadette van den Hoogen, Arvind Varsani, Nikos Vasilakis, Martin Verbeek, Victoria Wahl, Peter J. Walker, Hui Wang, Jianwei Wang, Xifeng Wang, Lin-Fa Wang, Taiyun Wei, Heather Wells, Anna E. Whitfield, John V. Williams, Yuri I. Wolf, Zhiqiang Wu, Xin Yang, Xinglou Yang, Xuejie Yu, Natalya Yutin, F. Murilo Zerbini, Tong Zhang, Yong-Zhen Zhang, Guohui Zhou, Xueping Zhou
ARCHIVES OF VIROLOGY
(2020)
Article
Virology
Alison Jane Basile, Kalanthe Horiuchi, Christin H. Goodman, Olga Kosoy, Amanda J. Panella, Jason O. Velez, Daniel M. Pastula, Aaron C. Brault, J. Erin Staples, Amanda E. Calvert
Summary: The study developed rapid and accurate IgM and IgG microsphere immunoassays for serologically identifying Heartland virus infections. Both tests showed sensitivities, specificities, and accuracies above 95% for detecting suspected infections, making them rapid and reliable first-line methods for serological identification.
JOURNAL OF CLINICAL VIROLOGY
(2021)
Article
Microbiology
Amanda E. Calvert, Kalanthe Horiuchi, Karen L. Boroughs, Yee T. Ong, Kimberly M. Anderson, Brad J. Biggerstaff, Mars Stone, Graham Simmons, Michael P. Busch, Claire Y-H Huang
Summary: The study demonstrates the persistence of IgM neutralizing antibodies after ZIKV infection, with IgM playing a predominant role in ZIKV neutralization and showing lower cross-reactivity compared to IgG.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Virology
Jens H. Kuhn, Scott Adkins, Bernard R. Agwanda, Rim Al Kubrusli, Sergey V. Alkhovsky, Gaya K. Amarasinghe, Tatjana Avsic-Zupanc, Maria A. Ayllon, Justin Bahl, Anne Balkema-Buschmann, Matthew J. Ballinger, Christopher F. Basler, Sina Bavari, Martin Beer, Nicolas Bejerman, Andrew J. Bennett, Dennis A. Bente, Eric Bergeron, Brian H. Bird, Carol D. Blair, Kim R. Blasdell, Dag-Ragnar Blystad, Jamie Bojko, Wayne B. Borth, Steven Bradfute, Rachel Breyta, Thomas Briese, Paul A. Brown, Judith K. Brown, Ursula J. Buchholz, Michael J. Buchmeier, Alexander Bukreyev, Felicity Burt, Carmen Buettner, Charles H. Calisher, Mengji Cao, Inmaculada Casas, Kartik Chandran, Remi N. Charrel, Qi Cheng, Yuya Chiaki, Marco Chiapello, Il-Ryong Choi, Marina Ciuffo, J. Christopher S. Clegg, Ian Crozier, Elena Dal Bo, Juan Carlos de la Torre, Xavier de Lamballerie, Rik L. de Swart, Humberto Debat, Nolwenn M. Dheilly, Emiliano Di Cicco, Nicholas Di Paola, Francesco Di Serio, Ralf G. Dietzgen, Michele Digiaro, Olga Dolnik, Michael A. Drebot, J. Felix Drexler, William G. Dundon, W. Paul Duprex, Ralf Durrwald, John M. Dye, Andrew J. Easton, Hideki Ebihara, Toufic Elbeaino, Koray Ergunay, Hugh W. Ferguson, Anthony R. Fooks, Marco Forgia, Pierre B. H. Formenty, Jana Franova, Juliana Freitas-Astua, Jingjing Fu, Stephanie Fuerl, Selma Gago-Zachert, George Fu Gao, Maria Laura Garcia, Adolfo Garcia-Sastre, Aura R. Garrison, Thomas Gaskin, Jean-Paul J. Gonzalez, Anthony Griffiths, Tony L. Goldberg, Martin H. Groschup, Stephan Guenther, Roy A. Hall, John Hammond, Tong Han, Jussi Hepojoki, Roger Hewson, Jiang Hong, Ni Hong, Seiji Hongo, Masayuki Horie, John S. Hu, Tao Hu, Holly R. Hughes, Florian Huettner, Timothy H. Hyndman, M. Ilyas, Risto Jalkanen, Daohong Jiang, Gilda B. Jonson, Sandra Junglen, Fujio Kadono, Karia H. Kaukinen, Michael Kawate, Boris Klempa, Jonas Klingstrom, Gary Kobinger, Igor Koloniuk, Hideki Kondo, Eugene V. Koonin, Mart Krupovic, Kenji Kubota, Gael Kurath, Lies Laenen, Amy J. Lambert, Stanley L. Langevin, Benhur Lee, Elliot J. Lefkowitz, Eric M. Leroy, Shaorong Li, Longhui Li, Jianrong Li, Huazhen Liu, Igor S. Lukashevich, Pie Maes, William Marciel de Souza, Marco Marklewitz, Sergio H. Marshall, Shin-Yi L. Marzano, Sebastien Massart, John W. McCauley, Michael Melzer, Nicole Mielke-Ehret, Kristina M. Miller, Tobi J. Ming, Ali Mirazimi, Gideon J. Mordecai, Hans-Peter Muehlbach, Elke Muehlberger, Rayapati Naidu, Tomohide Natsuaki, Jose A. Navarro, Sergey V. Netesov, Gabriele Neumann, Norbert Nowotny, Marcio R. T. Nunes, Alejandro Olmedo-Velarde, Gustavo Palacios, Vicente Pallas, Bernadett Palyi, Anna Papa, Sofia Paraskevopoulou, Adam C. Park, Colin R. Parrish, David A. Patterson, Alex Pauvolid-Correa, Janusz T. Paweska, Susan Payne, Carlotta Peracchio, Daniel R. Perez, Thomas S. Postler, Liying Qi, Sheli R. Radoshitzky, Renato O. Resende, Carina A. Reyes, Bertus K. Rima, Gabriel Robles Luna, Victor Romanowski, Paul Rota, Dennis Rubbenstroth, Luisa Rubino, Jonathan A. Runstadler, Sead Sabanadzovic, Amadou Alpha Sall, Maria S. Salvato, Rosemary Sang, Takahide Sasaya, Angela D. Schulze, Martin Schwemmle, Mang Shi, Xiaohong Shi, Zhengli Shi, Yoshifumi Shimomoto, Yukio Shirako, Stuart G. Siddell, Peter Simmonds, Manuela Sironi, Guy Smagghe, Sophie Smither, Jin-Won Song, Kirsten Spann, Jessica R. Spengler, Mark D. Stenglein, David M. Stone, Jari Sugano, Curtis A. Suttle, Amy Tabata, Ayato Takada, Shigeharu Takeuchi, David P. Tchouassi, Amy Teffer, Robert B. Tesh, Natalie J. Thornburg, Yasuhiro Tomitaka, Keizo Tomonaga, Noel Tordo, Baldwyn Torto, Jonathan S. Towner, Shinya Tsuda, Changchun Tu, Massimo Turina, Ioannis E. Tzanetakis, Janice Uchida, Tomio Usugi, Anna Maria Vaira, Marta Vallino, Bernadette van den Hoogen, Arvind Varsani, Nikos Vasilakis, Martin Verbeek, Susanne von Bargen, Jiro Wada, Victoria Wahl, Peter J. Walker, Lin-Fa Wang, Guoping Wang, Yanxiang Wang, Yaqin Wang, Muhammad Waqas, Taiyun Wei, Shaohua Wen, Anna E. Whitfield, John V. Williams, Yuri I. Wolf, Jiangxiang Wu, Lei Xu, Hironobu Yanagisawa, Caixia Yang, Zuokun Yang, F. Murilo Zerbini, Lifeng Zhai, Yong-Zhen Zhang, Song Zhang, Jinguo Zhang, Zhe Zhang, Xueping Zhou
Summary: In March 2021, the phylum Negarnaviricota was amended and expanded by four families, three subfamilies, 42 genera, and 200 species. Additionally, 39 species were renamed or moved, and seven species were abolished. This article presents the updated taxonomy of Negarnaviricota accepted by the ICTV.
ARCHIVES OF VIROLOGY
(2021)
Correction
Virology
Jens H. Kuhn, Scott Adkins, Bernard R. Agwanda, Rim Al Kubrusli, Sergey V. Alkhovsky, Gaya K. Amarasinghe, Tatjana Avsic-Zupanc, Maria A. Ayllon, Justin Bahl, Anne Balkema-Buschmann, Matthew J. Ballinger, Christopher F. Basler, Sina Bavari, Martin Beer, Nicolas Bejerman, Andrew J. Bennett, Dennis A. Bente, Eric Bergeron, Brian H. Bird, Carol D. Blair, Kim R. Blasdell, Dag-Ragnar Blystad, Jamie Bojko, Wayne B. Borth, Steven Bradfute, Rachel Breyta, Thomas Briese, Paul A. Brown, Judith K. Brown, Ursula J. Buchholz, Michael J. Buchmeier, Alexander Bukreyev, Felicity Burt, Carmen Buettner, Charles H. Calisher, Mengji Cao, Inmaculada Casas, Kartik Chandran, Remi N. Charrel, Qi Cheng, Yuya Chiaki, Marco Chiapello, Il-Ryong Choi, Marina Ciuffo, J. Christopher S. Clegg, Ian Crozier, Elena Dal Bo, Juan Carlos de la Torre, Xavier de Lamballerie, Rik L. de Swart, Humberto Debat, Nolwenn M. Dheilly, Emiliano Di Cicco, Nicholas Di Paola, Francesco Di Serio, Ralf G. Dietzgen, Michele Digiaro, Olga Dolnik, Michael A. Drebot, J. Felix Drexler, William G. Dundon, W. Paul Duprex, Ralf Durrwald, John M. Dye, Andrew J. Easton, Hideki Ebihara, Toufic Elbeaino, Koray Ergunay, Hugh W. Ferguson, Anthony R. Fooks, Marco Forgia, Pierre B. H. Formenty, Jana Franova, Juliana Freitas-Astua, Jingjing Fu, Stephanie Furl, Selma Gago-Zachert, George Fu Gao, Maria Laura Garcia, Adolfo Garcia-Sastre, Aura R. Garrison, Thomas Gaskin, Jean-Paul J. Gonzalez, Anthony Griffiths, Tony L. Goldberg, Martin H. Groschup, Stephan Guenther, Roy A. Hall, John Hammond, Tong Han, Jussi Hepojoki, Roger Hewson, Jiang Hong, Ni Hong, Seiji Hongo, Masayuki Horie, John S. Hu, Tao Hu, Holly R. Hughes, Florian Huettner, Timothy H. Hyndman, M. Ilyas, Risto Jalkanen, Daohong Jiang, Gilda B. Jonson, Sandra Junglen, Fujio Kadono, Karia H. Kaukinen, Michael Kawate, Boris Klempa, Jonas Klingstrom, Gary Kobinger, Igor Koloniuk, Hideki Kondo, Eugene V. Koonin, Mart Krupovic, Kenji Kubota, Gael Kurath, Lies Laenen, Amy J. Lambert, Stanley L. Langevin, Benhur Lee, Elliot J. Lefkowitz, Eric M. Leroy, Shaorong Li, Longhui Li, Jianrong Liu, Huazhen Liu, Igor S. Lukashevich, Piet Maes, William Marciel de Souza, Marco Marklewitz, Sergio H. Marshall, Shin-Yi L. Marzano, Sebastien Massart, John W. McCauley, Michael Melzer, Nicole Mielke-Ehret, Kristina M. Miller, Tobi J. Ming, Ali Mirazimi, Gideon J. Mordecai, Hans-Peter Muehlbach, Elke Muehlberger, Rayapati Naidu, Tomohide Natsuaki, Jose A. Navarro, Sergey V. Netesov, Gabriele Neumann, Norbert Nowotny, Marcio R. T. Nunes, Alejandro Olmedo-Velarde, Gustavo Palacios, Vicente Pallas, Bernadett Palyi, Anna Papa, Sofia Paraskevopoulou, Adam C. Park, Colin R. Parrish, David A. Patterson, Alex Pauvolid-Correa, Janusz T. Paweska, Susan Payne, Carlotta Peracchio, Daniel R. Perez, Thomas S. Postler, Liying Qi, Sheli R. Radoshitzky, Renato O. Resende, Carina A. Reyes, Bertus K. Rima, Gabriel Robles Luna, Victor Romanowski, Paul Rota, Dennis Rubbenstroth, Luisa Rubino, Jonathan A. Runstadler, Sead Sabanadzovic, Amadou Alpha Sall, Maria S. Salvato, Rosemary Sang, Takahide Sasaya, Angela D. Schulze, Martin Schwemmle, Mang Shi, Xiaohong Shi, Zhengli Shi, Yoshifumi Shimomoto, Yukio Shirako, Stuart G. Siddell, Peter Simmonds, Manuela Sironi, Guy Smagghe, Sophie Smither, Jin-Won Song, Kirsten Spann, Jessica R. Spengler, Mark D. Stenglein, David M. Stone, Jari Sugano, Curtis A. Suttle, Amy Tabata, Ayato Takada, Shigeharu Takeuchi, David P. Tchouassi, Amy Teffer, Robert B. Tesh, Natalie J. Thornburg, Yasuhiro Tomitaka, Keizo Tomonaga, Noel Tordo, Baldwyn Torto, Jonathan S. Towner, Shinya Tsuda, Changchun Tu, Massimo Turina, Ioannis E. Tzanetakis, Janice Uchida, Tomio Usugi, Anna Maria Vaira, Marta Vallino, Bernadette van den Hoogen, Arvind Varsani, Nikos Vasilakis, Martin Verbeek, Susanne von Bargen, Jiro Wada, Victoria Wahl, Peter J. Walker, Lin-Fa Wang, Guoping Wang, Yanxiang Wang, Yaqin Wang, Muhammad Waqas, Taiyun Wei, Shaohua Wen, Anna E. Whitfield, John V. Williams, Yuri I. Wolf, Jiangxiang Wu, Lei Xu, Hironobu Yanagisawa, Caixia Yang, Zuokun Yang, F. Murilo Zerbini, Lifeng Zhai, Yong-Zhen Zhang, Song Zhang, Jinguo Zhang, Zhe Zhang, Xueping Zhou
ARCHIVES OF VIROLOGY
(2021)
Article
Virology
Amanda E. Calvert, Susan L. Bennett, Ann R. Hunt, Rachel H. Fong, Benjamin J. Doranz, John T. Roehrig, Carol D. Blair
Summary: A broadly cross-reactive E1-specific MAb has been found to protect animals from Venezuelan equine encephalitis virus infection and inhibit virus replication and cell fusion in vitro. The amino acid residue N100 in the fusion loop of the E1 protein is identified as critical for binding. Further research is needed to explore the potential of broadly cross-reactive MAbs for vaccine and therapeutic development.
Article
Infectious Diseases
Benjamin Skinner, Sierra Mikula, Brent S. Davis, Jordan A. Powers, Holly R. Hughes, Amanda E. Calvert
Summary: Cache Valley virus is a mosquito-borne virus known to cause fetal death and severe malformations in livestock, and has recently emerged as a potential viral pathogen causing severe disease in humans. Developing anti-CVV murine monoclonal antibodies can improve diagnostic capacity to detect CVV infections accurately and quickly.
PLOS NEGLECTED TROPICAL DISEASES
(2022)
Article
Microbiology
Jordan A. Powers, Benjamin Skinner, Brent S. Davis, Brad J. Biggerstaff, Lucy Robb, Elizabeth Gordon, Amanda E. Calvert, Gwong-Jen Chang
Summary: This study describes the generation of 39 stable cell lines secreting flaviviral virus-like particles (VLP) and nonstructural protein 1 (NS1) in HEK-293 cells, which will expand serological diagnostic testing for flaviviruses worldwide.
MICROBIOLOGY SPECTRUM
(2022)
Article
Virology
Steven M. Erb, Siritorn Butrapet, John T. Roehrig, Claire Y-H Huang, Carol D. Blair
Summary: Dengue viruses are transmitted to humans by mosquitoes. A study found that adaptive mutations in the virus can increase the infection rate in mosquitoes.
Article
Virology
Jens H. Kuhn, Scott Adkins, Sergey Alkhovsky, Tatjana Avsic-Zupanc, Maria A. Ayllon, Justin Bahl, Anne Balkema-Buschmann, Matthew J. Ballinger, Martina Bandte, Martin Beer, Nicolas Bejerman, Eric Bergeron, Nadine Biedenkopf, Laurent Bigarre, Carol D. Blair, Kim R. Blasdell, Steven B. Bradfute, Thomas Briese, Paul A. Brown, Remy Bruggmann, Ursula J. Buchholz, Michael J. Buchmeier, Alexander Bukreyev, Felicity Burt, Carmen Buettner, Charles H. Calisher, Thierry Candresse, Jeremy Carson, Inmaculada Casas, Kartik Chandran, Remi N. Charrel, Yuya Chiaki, Anya Crane, Mark Crane, Laurent Dacheux, Elena Dal Bo, Juan Carlos de la Torre, Xavier de Lamballerie, William M. de Souza, Rik L. de Swart, Nolwenn M. Dheilly, Nicholas Di Paola, Francesco Di Serio, Ralf G. Dietzgen, Michele Digiaro, J. Felix Drexler, W. Paul Duprex, Ralf Duerrwald, Andrew J. Easton, Toufc Elbeaino, Koray Ergunay, Guozhong Feng, Claudette Feuvrier, Andrew E. Firth, Anthony R. Fooks, Pierre B. H. Formenty, Juliana Freitas-Astua, Selma Gago-Zachert, Maria Laura Garcia, Adolfo Garcia-Sastre, Aura R. Garrison, Scott E. Godwin, Jean-Paul J. Gonzalez, Joelle Gouy de Bellocq, Anthony Grifths, Martin H. Groschup, Stephan Gunther, John Hammond, Jussi Hepojoki, Melanie M. Hierweger, Seiji Hongo, Masayuki Horie, Hidenori Horikawa, Holly R. Hughes, Adam J. Hume, Timothy H. Hyndman, Daohong Jiang, Gilda B. Jonson, Sandra Junglen, Fujio Kadono, David G. Karlin, Boris Klempa, Jonas Klingstrom, Michel C. Koch, Hideki Kondo, Eugene Koonin, Jarmila Krasova, Mart Krupovic, Kenji Kubota, Ivan Kuzmin, Lies Laenen, Amy J. Lambert, Jianrong Li, Jun-Min Li, Francois Liefrig, Igor S. Lukashevich, Dongsheng Luo, Piet Maes, Marco Marklewitz, Sergio H. Marshall, Shin-Yi L. Marzano, John W. McCauley, Ali Mirazimi, Peter G. Mohr, Nick J. G. Moody, Yasuaki Morita, Richard N. Morrison, Elke Muhlberger, Rayapati Naidu, Tomohide Natsuaki, Jose A. Navarro, Yutaro Neriya, Sergey Netesov, Gabriele Neumann, Norbert Nowotny, Francisco M. Ochoa-Corona, Gustavo Palacios, Laurane Pallandre, Vicente Pallas, Anna Papa, Sofa Paraskevopoulou, Colin R. Parrish, Alex Pauvolid-Correa, Janusz T. Paweska, Daniel R. Perez, Florian Pfaf, Richard K. Plemper, Thomas S. Postler, Francoise Pozet, Sheli R. Radoshitzky, Pedro L. Ramos-Gonzalez, Marius Rehanek, Renato O. Resende, Carina A. Reyes, Victor Romanowski, Dennis Rubbenstroth, Luisa Rubino, Artemis Rumbou, Jonathan A. Runstadler, Melanie Rupp, Sead Sabanadzovic, Takahide Sasaya, Heike Schmidt-Posthaus, Martin Schwemmle, Torsten Seuberlich, Stephen R. Sharpe, Mang Shi, Manuela Sironi, Sophie Smither, Jin-Won Song, Kirsten M. Spann, Jessica R. Spengler, Mark D. Stenglein, Ayato Takada, Robert B. Tesh, Jana Tesikova, Natalie J. Thornburg, Nicole D. Tischler, Yasuhiro Tomitaka, Keizo Tomonaga, Noel Tordo, Kenta Tsunekawa, Massimo Turina, Ioannis E. Tzanetakis, Anna Maria Vaira, Bernadette van den Hoogen, Bert Vanmechelen, Nikos Vasilakis, Martin Verbeek, Susanne von Bargen, Jiro Wada, Victoria Wahl, Peter J. Walker, Anna E. Whitfeld, John Williams, Yuri Wolf, Junki Yamasaki, Hironobu Yanagisawa, Gongyin Ye, Yong-Zhen Zhang, Arnfnn Lodden Okland
Summary: This article presents the updated taxonomy of Negarnaviricota, including the addition of new families, genera, and species, renaming and moving of species, and correction of misspelled names as accepted by the International Committee on Taxonomy of Viruses (ICTV).
ARCHIVES OF VIROLOGY
(2022)
Review
Microbiology
Carol D. Blair
Summary: Arboviruses, transmitted by arthropods like mosquitoes, can cause serious diseases in humans and other vertebrates. Understanding the interactions between mosquitoes and the viruses they transmit is crucial for controlling transmission. Mosquitoes have innate immune responses that can modulate virus replication, and studying these responses can lead to better methods for interrupting the transmission cycle.
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
(2023)
Review
Entomology
Holly R. Hughes, Joan L. Kenney, Amanda E. Calvert
Summary: Cache Valley virus (CVV) is a mosquito-borne virus that can cause severe diseases, including fetal malformations, in both ruminants and humans. Despite being a public health and agricultural concern, there is limited scientific research on CVV. More studies are needed to understand its geographic distribution, ecological cycle, seroprevalence in humans and animals, and its potential as a human teratogen.
JOURNAL OF MEDICAL ENTOMOLOGY
(2023)
Article
Microbiology
Jordan A. Powers, Karen L. Boroughs, Sierra Mikula, Christin H. Goodman, Emily H. Davis, Elisa M. Thrasher, Holly R. Hughes, Brad J. Biggerstaff, Amanda E. Calvert
Summary: New antibodies have been developed for the detection of California serogroup viruses (CSGVs), which can replace human-positive control sera in diagnostic tests. A human-murine chimeric antibody was engineered by combining the variable regions of a murine antibody and the constant region of human IgM. This engineered antibody showed equivalent reactivity to CSGVs and can be used in diagnostic serological techniques.
MICROBIOLOGY SPECTRUM
(2023)