期刊
VIROLOGY
卷 401, 期 2, 页码 322-332出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2010.02.028
关键词
Astrovirus; Virus release; Capsid protein processing; Caspases
类别
资金
- CONACyT [44884-Q, 79574]
- DGAPA-UNAM [IN226106, CRP.LA/MEX03-01]
- ICGB-OPS-RELAB
Caspases (Casp) activity has been associated with the intracellular proteolytic processing of the structural protein to yield the mature capsid formed by VP70 and with the cell release of human astrovirus (HAstV). This work describes the role of individual Casp on these events. The activity of initiator (-8, -9) and executioner (-3/7) Casp was clearly detected at 12 h post-infection. All these proteases were able to cleave VP90 in an in vitro assay, but this processing was blocked in cells transfected with siRNA against Casp-3, -9, but not against Casp-8. In contrast, virus release, observed in the absence of cell lysis, was more drastically affected by either silencing Casp-3 or in the presence of the inhibitor Ac-DEVD-CHO. Cleavage of VP90 to yield VP70 was mapped at motif TYVD657. These data indicate that the processing of VP90 and the release of HAstV from the cell are two Casp-related, but apparently independent, events. (C) 2010 Elsevier Inc. All rights reserved.
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