Article
Microbiology
Lishuang Wang, Peijie Tian, Xiuling Yang, Xueping Zhou, Songbai Zhang, Chun Li, Xuehui Yang, Yong Liu
Summary: Pepper vein yellows virus (PeVYV) is a new Polerovirus identified in Chinese pepper plants, causing symptoms such as intervein yellow staining and leaf curl. The P0 protein of PeVYV plays a role in pathogenicity, post-transcriptional gene silencing (PTGS) activity, and nuclear localization, with the F-box motif being crucial for symptom determination and PTGS inhibition. This study highlights the importance of further research on the F-box domain amino acids of the P0 protein in the interaction between PeVYV and plants.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Virology
Liming Liu, Qian Ren, Bin Peng, Baoshan Kang, Huijie Wu, Qinsheng Gu
Summary: In this study, the complete genome sequence of Melon aphid-borne yellows virus (MABYV) was obtained, and an infectious cDNA clone was constructed. The clone could cause yellowing symptoms in bottle guard, cucumber, and muskmelon plants, and could be transmitted by aphids. Point mutations in certain regions of the genome resulted in low viral accumulation and failed to induce symptoms.
Article
Biology
Xiao-Yan Zhang, Yuan-Yuan Li, Ying Wang, Da-Wei Li, Jia-Lin Yu, Cheng-Gui Han
Summary: Polerovirus P0 proteins play multifunctional roles with viral suppressor of RNA silencing (VSR) functions, as well as the ability to induce cell death in infiltrated regions of N. benthamiana. The study compared the features of P0 proteins encoded by different Brassica yellows virus (BrYV) genotypes, identified key residues for inducing cell death and revealed synergistic interactions with PEMV 2. This research provides theoretical guidance for controlling viral diseases caused by poleroviruses.
Article
Biochemistry & Molecular Biology
Xin Zhang, Mamun-Or Rashid, Tian-Yu Zhao, Yuan-Yuan Li, Meng-Jun He, Ying Wang, Da-Wei Li, Jia-Lin Yu, Cheng-Gui Han
Summary: The C-terminal truncation of P0 proteins compromises the systemic infection of BrYV and PLRV. While the truncation does not affect the local VSR activity of P0 proteins, the systemic infection cannot be rescued by transient or ectopic expression of VSR proteins. Additionally, systemic infection may be independent of the VSR activity of P0.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Miryam Perez-Canamas, Monica Bustos, Victoria Puertolas, Yolanda Castello, Sofia Peiro, Carmen Hernandez
Summary: In this study, the authors investigate the structural and functional properties of a viral suppressor of RNA silencing (VSR) encoded by Pepper vein yellows virus 5 (PeVYV-5). They find that PeVYV-5 P0 is a potent VSR that induces the degradation of key components of RNA silencing machinery. The sequencing analysis of new viral isolates reveals limited heterogeneity in the P0 protein, suggesting negative selection. This study provides important insights into the role of VSRs in plant virus control.
Article
Plant Sciences
Jiahui Zhang, Huiyuan Li, Xue Zhong, Jinfu Tian, Arnaud Segers, Lanqin Xia, Frederic Francis
Summary: Grain aphid (Sitobion miscanthi) is a dominant and devastating insect pest in wheat, causing significant losses to wheat production. This study identified and characterized a potential RNAi target gene (SmDSR33) encoding a salivary protein in grain aphids. Transgenic wheat lines expressing dsRNA for targeted silencing of SmDSR33 were generated, and the feeding experiments showed attenuated expression levels of SmDSR33 in aphids and reduced fecundity, survival, and reproduction. Altered feeding behaviors were observed in electroneurography assays. The results suggest that SmDSR33 can be an effective RNAi target for wheat aphid control.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Entomology
Jun Jiang, Anna Erickson, Wenjie Qiao, Emilyn E. Matsumura, Bryce W. Falk
Summary: This study demonstrates that Flock House virus (FHV) can infect multiple aphid species, and engineered FHV particles can decrease the expression levels of key genes in aphids and even cause their death. This research contributes to the development of RNA interference-based pest control methods.
JOURNAL OF PEST SCIENCE
(2023)
Article
Plant Sciences
Han-Guang Wen, Jian-Hua Zhao, Bo-Sen Zhang, Feng Gao, Xue-Ming Wu, Yong-Sheng Yan, Jie Zhang, Hui-Shan Guo
Summary: This study developed a microbe-induced gene silencing technology and demonstrated interspecies RNA interference between rhizospheric microorganisms. By targeting fungal genes, researchers showed the effectiveness of MIGS in inhibiting fungal growth and protecting crops against fungal pathogens.
Article
Plant Sciences
Licheng Wang, Wenbao Chen, Huan Ma, Jingyuan Li, Xingan Hao, Yunfeng Wu
Summary: The phytoplasma encoded RSS SWP16 inhibits RNA silencing in plants, leading to viral infection and providing insight into pathogen-plant interaction mechanisms.
Article
Entomology
Farman Ullah, Hina Gul, Kaleem Tariq, Muhammad Hafeez, Nicolas Desneux, Xiwu Gao, Dunlun Song
Summary: The Ecdysone receptor (EcR) is an essential gene for aphid growth and development. This study demonstrates the potential of using RNA interference (RNAi) technology to control aphids by silencing the EcR gene. The results suggest that EcR could be a target gene for the development of RNAi-based bio-aphicide.
ENTOMOLOGIA GENERALIS
(2022)
Article
Biochemistry & Molecular Biology
Robert Pertermann, Ralph Peter Golbik, Selvaraj Tamilarasan, Torsten Gursinsky, Selma Gago-Zachert, Vitantonio Pantaleo, Iris Thondorf, Sven-Erik Behrens
Summary: Plant viruses can produce suppressor proteins that inhibit RNA silencing, a mechanism of antiviral plant immunity. These proteins can bind to virus-derived siRNAs and miRNAs, influencing cellular gene expression. The study found that a specific viral suppressor protein, p19, can bind to different miRNAs with varying affinities due to specific features in the RNA and protein molecules. These findings provide important insights into the efficiency and adaptability of the plant antiviral immune response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
M. P. Rabadan, M. Juarez, P. Gomez
Summary: Understanding the emergence and prevalence of viral diseases in crops requires systematic monitoring and analysis. This study monitored six aphid-transmitted viruses in melon and zucchini crops in Spain for 10 consecutive cropping seasons. The most prevalent viruses were CABYV and WMV, and mixed infections seemed to affect the evolutionary epidemiology of these viral diseases. Genetic characterization of CABYV and WMV populations revealed variation and structure in CABYV populations, while WMV populations showed no genetic differentiation and were under purifying selection.
Article
Microbiology
Shuangchao Wang, Jingze Zhang, Clement Nzabanita, Mingming Zhang, Jianhua Nie, Lihua Guo
Summary: The study investigated the function of an uncharacterized protein encoded by FgHV1, p20, which was identified as a novel fungal RNA silencing suppressor in an agro-infiltration assay. The research elucidated the mechanism of RNA silencing suppression by p20, revealing its capability to bind single-strand sRNA in a non-sequence-specific manner from both virus and non-virus sources.
Article
Virology
Ken-Der Wang, Mansour A. Dughbaj, Tan Tri Nguyen, Tiffany Quyen Y. Nguyen, Shyamal Oza, Kevin Valdez, Priscilla Anda, Jacob Waltz, Melanie Ann Sacco
Summary: The protein P0 serves as a viral suppressor of RNA silencing (VSR) for poleroviruses, but also elicits the hypersensitive response (HR) in specific Nicotiana species. Deletions and mutations in the N-terminus and C-terminus of P0 affected both HR elicitation and VSR activity. Two conserved blocks of amino acid residues were associated with HR, and a specific mutation in block 1 enhanced HR. Deletion or mutation of block 2 preserved VSR activity but impaired HR, allowing the virus to escape from resistance in Nicotiana glutinosa.
Article
Biochemistry & Molecular Biology
Jiyeong Choi, Samira Pakbaz, Luz Marcela Yepes, Elizabeth Jeannette Cieniewicz, Corinne Schmitt-Keichinger, Rossella Labarile, Serena Anna Minutillo, Michelle Heck, Jian Hua, Marc Fuchs
Summary: In this study, researchers identified viral suppressors of RNA silencing encoded by Grapevine fanleaf virus (GFLV) and demonstrated their ability to reverse systemic RNA silencing and modify host gene expression. These findings reveal the potential interference mechanism of GFLV in the plant antiviral RNA silencing pathways during infection.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2023)
Article
Biochemistry & Molecular Biology
Meng Yang, Asigul Ismayil, Zhihao Jiang, Yan Wang, Xiyin Zheng, Liming Yan, Yiguo Hong, Dawei Li, Yule Liu
Summary: The study reveals that the replicase gamma a of Barley stripe mosaic virus can inhibit vacuolar acidification and suppress autophagic degradation to promote viral infection in plants. The disruption of vacuolar acidification seems to be a common viral counter-defense strategy employed by multiple viruses, highlighting the importance of functional vacuolar acidification in plant antiviral defense.
Article
Plant Sciences
Zhaolei Li, Xinxin Yang, Wenli Li, Zhiyan Wen, Jiangning Duan, Zhihao Jiang, Dingliang Zhang, Xialin Xie, Xueting Wang, Fangfang Li, Dawei Li, Yongliang Zhang
Summary: In this study, we found that SAMDC3 is involved in defense against BSMV infection by regulating the abundance of the γb protein. This finding contributes to our understanding of how a plant host deploys the ubiquitin-proteasome system to mount defenses against viral infections.
Article
Multidisciplinary Sciences
Zongyu Gao, Dingliang Zhang, Xiaoling Wang, Xin Zhang, Zhiyan Wen, Qianshen Zhang, Dawei Li, Savithramma P. Dinesh-Kumar, Yongliang Zhang
Summary: The study reveals that beet black scorch virus activates MAPK signaling in plants and the viral coat protein counteracts MAPK-mediated defense. This finding highlights the importance of MAPK cascades in plant defense against viral infections.
NATURE COMMUNICATIONS
(2022)
Article
Biology
Xiao-Dong Fang, Qiang Gao, Ying Zang, Ji-Hui Qiao, Dong-Min Gao, Wen-Ya Xu, Ying Wang, Dawei Li, Xian-Bing Wang
Summary: Host factor casein kinase 1 (CK1) regulates liquid-liquid phase separation (LLPS) of viral proteins during negative-sense RNA (NSR) virus infection.
Article
Biochemistry & Molecular Biology
Ning Yue, Zhihao Jiang, Xuan Zhang, Zhenggang Li, Xueting Wang, Zhiyan Wen, Zongyu Gao, Qinglin Pi, Yongliang Zhang, Xian-Bing Wang, Chenggui Han, Jialin Yu, Dawei Li
Summary: Viral replication and movement are closely related, but the molecular mechanisms controlling the transition between replication and movement are mostly unknown. This study discovered that palmitoylation of the BSMV γb protein in tobacco regulates viral replication and movement.
Article
Biochemistry & Molecular Biology
Xin Zhang, Mamun-Or Rashid, Tian-Yu Zhao, Yuan-Yuan Li, Meng-Jun He, Ying Wang, Da-Wei Li, Jia-Lin Yu, Cheng-Gui Han
Summary: The C-terminal truncation of P0 proteins compromises the systemic infection of BrYV and PLRV. While the truncation does not affect the local VSR activity of P0 proteins, the systemic infection cannot be rescued by transient or ectopic expression of VSR proteins. Additionally, systemic infection may be independent of the VSR activity of P0.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Multidisciplinary
Zhang Xiao-yan, Peng Yan-mei, Xiang Hai-ying, Wang Ying, Li Da-Wei, Yu Jia-lin, Han Cheng-gui
Summary: We reported the distribution and prevalence levels of BrYV genotypes in crucifer crops in China. The study revealed a widespread prevalence of BrYV in China, with common mixed infections with other viruses.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2022)
Article
Plant Sciences
Dong Deng, Suli Sun, Wenqi Wu, Canxing Duan, Zhaoli Wang, Shilong Zhang, Zhendong Zhu
Summary: In this study, the causal agent of common bean powdery mildew was identified as E. vignae, and resistance of common bean cultivars was evaluated, resulting in the identification of 15 resistant or segregant cultivars.
Article
Biochemistry & Molecular Biology
Dong Deng, Suli Sun, Wenqi Wu, Chao Xiang, Canxing Duan, Dongmei Yu, Xuehong Wu, Zhendong Zhu
Summary: This study evaluated the disease resistance and molecular variations of mutants derived from two elite vegetable pea cultivars, SJ1 and CW8. The mutants showed resistant variations in Fusarium wilt and powdery mildew, and genetic variations were confirmed using SSR markers. The phylogenetic analysis divided the mutants into distinct groups with genetic differences. The findings provide valuable information for pea production and breeding.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Yusheng Xia, Baobao Wang, Lihong Zhu, Wenqi Wu, Suli Sun, Zhendong Zhu, Xinhai Li, Jianfeng Weng, Canxing Duan
Summary: This study comprehensively characterized and mapped the genetic basis of FER resistance in maize using RIL and CSSL populations. A total of 17 QTL were identified, with qFER1.03 having the strongest effect on FER resistance. Further analysis revealed that qFER1.03 differed from other resistance QTL on chromosome 1 and identified a candidate gene, GRMZM2G017792 (MPK3), in this region.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Dingliang Zhang, Zongyu Gao, He Zhang, Yizhou Yang, Xinxin Yang, Xiaofei Zhao, Hailong Guo, Ugrappa Nagalakshmi, Dawei Li, Savithramma P. Dinesh-Kumar, Yongliang Zhang
Summary: This article investigates a novel MAPK-AL7 module that promotes NLR-mediated immunity by inhibiting the transcription of ROS scavenging genes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Microbiology
Si Liu, Yanhong Han, Wan-Xiang Li, Shou-Wei Ding
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
(2023)
Article
Biochemistry & Molecular Biology
Qianshen Zhang, Zhiyan Wen, Xin Zhang, Jiajie She, Xiaoling Wang, Zongyu Gao, Ruiqi Wang, Xiaofei Zhao, Zhen Su, Zhen Li, Dawei Li, Xiaofeng Wang, Yongliang Zhang
Summary: The study employed TurboID-based proximity labeling to investigate the replication of a plant virus and identified RETICULON-LIKE PROTEIN B2 as a proviral factor in viral replication complex formation.
Article
Plant Sciences
Li-Juan Zhu, Chong-Tao Zhang, Ya-Ni Bai, Chao-Yang Jiang, Shi-Yun He, Qing Chang, Qian Xu, Yan-Hong Han
Summary: Jasmine virus H (JaVH) is a newly discovered pathogen causing yellow mosaic symptoms on jasmine plants. Its complete genome sequences were determined from samples collected in China, and the 3 ' untranslated region (3 ' UTR) of the virus was found to play a crucial role in viral RNA accumulation.
PHYTOPATHOLOGY RESEARCH
(2023)
Article
Multidisciplinary Sciences
Xin Tong, Jia-Jia Zhao, Ya-Lan Feng, Jing-Ze Zou, Jian Ye, Junfeng Liu, Chenggui Han, Dawei Li, Xian-Bing Wang
Summary: Symptom recovery is achieved through a balanced arms race between viruses and plants. A small peptide called VISP1 can mediate the autophagic degradation of viral silencing suppressors, thus maintaining the balance between virus pathogenicity and plant immunity.
NATURE COMMUNICATIONS
(2023)
Article
Virology
Naiqing Xu, Xinen Tang, Xin Wang, Miao Cai, Xiaowen Liu, Xiaolong Lu, Shunlin Hu, Min Gu, Jiao Hu, Ruyi Gao, Kaituo Liu, Yu Chen, Xiufan Liu, Xiaoquan Wang
Summary: This study found that the H9N2 subtype avian influenza virus has a high airborne transmissibility, while the H7N9 virus does not. The Hemagglutinin protein of the H9N2 virus was found to play a key role in replication, stability, and airborne transmission.
Article
Virology
Samar S. Ewies, Sabry M. Tamam, Ahmed S. Abdel-Moneim, Sherin R. Rouby
Summary: Contagious ecthyma (CE) is a highly contagious viral disease of sheep and goats worldwide. The study provided a clinical description of CE and screened for genetic variation in the B2L gene. Infected sheep exhibited anorexia and oral lesions, while inoculated chicken embryos showed pock lesions. The B2L gene was successfully amplified and found to be highly conserved.
Article
Virology
Yigal Farnoushi, Dan Heller, Avishai Lublin
Summary: In recent years, new variants of avian reovirus (ARV) have caused a variety of symptoms in chickens worldwide, including viral arthritis/tenosynovitis. This study analyzed emerging ARV variants in Israel and found significant genetic diversity. Most ARV isolates in Israel belonged to genotypic cluster 5 (GC5). The study suggests that Israel has not experienced the emergence of new ARV variants since the introduction of the live vaccine (ISR-7585), but ongoing monitoring is needed due to the continuous emergence of ARV variants.
Article
Virology
Shigeru Tajima, Michiyo Kataoka, Yuki Takamatsu, Hideki Ebihara, Chang-Kweng Lim
Summary: Yokose virus (YOKV), a bat-associated flavivirus, was found to replicate at a slower rate in mosquito cells compared to other mosquito-borne flaviviruses. Specific nucleotide mutations in the virus were identified to enhance its proliferation ability in mosquito cells.
Article
Virology
Alejandra Borjabad, Baojun Dong, Wei Chao, David J. Volsky, Mary Jane Potash
Summary: This study investigated HIV brain disease using a mouse model, and found that poly I:C can reverse associated cognitive impairment and reduce virus burden. The results also revealed transcriptional changes related to neuronal function and innate immune responses.
Article
Virology
Ching-Hung Lin, Feng-Cheng Hsieh, Meilin Wang, Chieh Hsu, Hsuan-Wei Hsu, Chun-Chun Yang, Cheng-Yao Yang, Hung-Yi Wu
Summary: This study demonstrates that the synthesis of coronavirus subgenomic mRNA is not solely determined by the sequence homology between the leader TRS and TRS-B, but also by the disassociation of the coronavirus polymerase from the viral genome. This finding provides a new insight into the transcription mechanism of coronaviruses.
Article
Virology
Nicholas S. Kron, Benjamin W. Neuman, Sathish Kumar, Patricia L. Blackwelder, Dayana Vidal, Delphina Z. Walker-Phelan, Patrick D. I. Gibbs, Lynne A. Fieber, Michael C. Schmale
Summary: Two recent studies documented the genome of a novel virus in marine animals, finding that the virus is widespread in apparently healthy animals but not highly expressed in neurons. The studies also identified viral replication factories and high levels of defective genomes in chronically infected animals.
Article
Virology
Andrew M. Ramey, Laura C. Scott, Christina A. Ahlstrom, Evan J. Buck, Alison R. Williams, Mia Kim Torchetti, David E. Stallknecht, Rebecca L. Poulson
Summary: We successfully detected and characterized highly pathogenic avian influenza viruses in hunter-harvested wild waterfowl samples from western Alaska. Genomic analysis revealed three independent viral introductions into Alaska. Our findings demonstrate the utility and potential limitations of using molecular processing approaches directly on original swab samples for viral research and monitoring.
Article
Virology
Ting Gong, Dongdong Wu, Yongzhi Feng, Xing Liu, Qi Gao, Xiaoyu Zheng, Zebu Song, Heng Wang, Guihong Zhang, Lang Gong
Summary: This study discovered that quercetin can inhibit PEDV replication both in vivo and in vitro, and alleviate the clinical symptoms and intestinal injury caused by the virus. This provides a new direction for the development of PED antiviral drugs.
Article
Virology
Min Zhu, Hao Zeng, Jianqiao He, Yaohui Zhu, Pingping Wang, Jianing Guo, Jinfan Guo, Huabo Zhou, Yifeng Qin, Kang Ouyang, Zuzhang Wei, Weijian Huang, Ying Chen
Summary: The reassortment between avian H9N2 and Eurasian avian-like (EA) H1N1 viruses may have potentially changed from avian-to-mammals adaptation. This study found that the introduction of EA H1N1 internal genes into H9N2 virus restored the replication capability and resulted in extreme virulence in some cases. This raises new concerns for public health due to the possible coexistence of H9N2 and EA H1N1 viruses in dogs.
