期刊
VIROLOGY
卷 394, 期 1, 页码 82-90出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2009.08.012
关键词
Hepatitis C virus; JFH-1; IRES-mediated translation; Transacting factors; Antivirals; Peptides
类别
资金
- National Institutes of Health [AI-45733, AI-38056, AI-45188]
Hepatitis C virus (HCV) infection frequently leads to chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma. There is no effective therapy or vaccine available to HCV-infected patients other than interferon-ribavarin combination, which is effective in a relatively small percentage of infected patients. Our previous results have shown that a synthetic peptide (LAP) corresponding to the N-terminal 18 amino acids of the Lupus autoantigen (La) was a potent inhibitor of HCV IRES-mediated translation. We demonstrate here that LAP efficiently blocks HCV replication of infectious JFH1 Virus in cell culture. Our data Suggest that LAP forms complexes with IRES-transacting factors (ITAFs) PTB and PCBP2. LAP-mediated inhibition of HCV IRES-mediated translation in vitro could be fully rescued by recombinant PCB and PCBP2. Also transient expression of PTB / PCBP2 combination significantly restores HCV replication in LAP-inhibited Cultures. These results Suggest that ITAFs could be potential targets to block HCV replication. (C) 2009 Elsevier Inc. All rights reserved.
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