Article
Medicine, Research & Experimental
Grant R. Campbell, Jia Zhuang, Gang Zhang, Igor Landa, Luke J. Kubiatowicz, Diana Dehaini, Ronnie H. Fang, Liangfang Zhang, Stephen A. Spector
Summary: This study demonstrates that DIABLO/SMAC mimetic loaded TNP have outstanding neutralizing breadth and potency against HIV-1, selectively killing HIV-1-infected cells through autophagy-dependent apoptosis with no off-target or cytotoxic effects on bystander cells. Genetic inhibition of early stages of autophagy abolishes this effect, indicating the potential of TNP as therapeutic agents for HIV-1 treatment.
Article
Multidisciplinary Sciences
Edurne Rujas, Hong Cui, Jonathan Burnie, Clare Burn Aschner, Tiantian Zhao, Sara Insausti, Krithika Muthuraman, Anthony Semesi, Jasper Ophel, Jose L. Nieva, Michael S. Seaman, Christina Guzzo, Bebhinn Treanor, Jean-Philippe Julien
Summary: In this study, HIV-1 bNAbs were engineered by directly fusing their Fab fragments to the human apoferritin light chain, resulting in a multispecific and avid molecule. The molecule exhibited high neutralization potency against a broad panel of HIV-1 pseudoviruses and demonstrated IgG-like bioavailability in vivo through Fc receptor modulation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Immunology
Romina Cecilia Russi, Diego del Balzo, Ivana Gabriela Reidel, Mariano Alonso Bivou, Noelia Flor, Agustin Lujan, Diego Sanchez, Maria Teresa Damiani, Carolina Veaute
Summary: This study evaluated a vaccine based on a fragment of the Polymorphic membrane protein D (FPmpD) of C. trachomatis as an immunogen using a DNA prime-protein boost strategy. The results showed that both free recombinant FPmpD and recombinant FPmpD formulated with adjuvants increased the titers of anti-rFPmpD antibodies in mice and induced a rapid and robust humoral immune response upon Ct challenge. However, the adjuvant-containing vaccine formulations were more effective in reducing Ct shedding and preventing immunopathology compared to the vaccine with free rFPmpD.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Chemistry, Physical
Nahid Akhtar, Amit Joshi, Joginder Singh, Vikas Kaushik
Summary: The study aimed to design a novel potent multi-epitope HCMV vaccine by predicting epitopes on target envelope glycoproteins, conducting molecular docking and population coverage analysis. A 283 amino acid long vaccine was successfully designed that is soluble, stable, and non-allergenic, with potential efficacy and safety further requiring validation through in vivo studies.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Chemistry, Multidisciplinary
Xiaoqian Xin, Yifeng Liu, Lei Guo, Hui Wang, Daiqiang Lu, Yaotian Chang, Mingming Wan, Yong Zhang, Yaming Shan, Qiao Zhang, Xiaowen Liu, Feng Gao
Summary: The HIV virus poses a major threat to human health with over 84 million people infected. Developing a vaccine has been challenging due to the high genetic variation of the virus. However, a new HIV-1 envelope glycoprotein nanoparticle vaccine has been designed using amphiphilic polymers, which shows potent and broad neutralizing activities against multiple HIV-1 subtypes. Additionally, this vaccine remains stable under different storage conditions and can potentially be applied to other protein-based vaccines.
Article
Immunology
Blandine Noailly, Melyssa Yaugel-Novoa, Justine Werquin, Fabienne Jospin, Daniel Drocourt, Thomas Bourlet, Nicolas Rochereau, Stephane Paul
Summary: Broadly neutralizing antibodies (bNAbs) show promising potential for HIV-1 prevention. Different bNAb isotypes have varying abilities in viral neutralization and ADCC-like activity. These findings contribute to the search for new treatments and antibody-based vaccines.
Article
Medicine, General & Internal
Glenda E. Gray, Linda-Gail Bekker, Fatima Laher, Mookho Malahleha, Mary Allen, Zoe Moodie, Nicole Grunenberg, Yunda Huang, Doug Grove, Brittany Prigmore, Jia J. Kee, David Benkeser, John Hural, Craig Innes, Erica Lazarus, Graeme Meintjes, Nivashnee Naicker, Dishiki Kalonji, Maphoshane Nchabeleng, Modulakgotla Sebe, Nishanta Singh, Philip Kotze, Sheetal Kassim, Thozama Dubula, Vimla Naicker, William Brumskine, Cleon N. Ncayiya, Amy M. Ward, Nigel Garrett, Girisha Kistnasami, Zakir Gaffoor, Pearl Selepe, Philisiwe B. Makhoba, Matsontso P. Mathebula, Pamela Mda, Tania Adonis, Katlego S. Mapetla, Bontle Modibedi, Tricia Philip, Gladys Kobane, Carter Bentley, Shelly Ramirez, Simbarashe Takuva, Megan Jones, Mpho Sikhosana, Millicent Atujuna, Michele Andrasik, Nima S. Hejazi, Adrian Puren, Lubbe Wiesner, Sanjay Phogat, Carlos Diaz Granados, Marguerite Koutsoukos, Olivier van der Meeren, Susan W. Barnett, Niranjan Kanesa-Thasan, James G. Kublin, M. Juliana McElrath, Peter B. Gilbert, Holly Janes, Lawrence Corey
Summary: The ALVAC-gp120 vaccine regimen did not prevent HIV-1 infection among participants in South Africa, despite previous evidence of immunogenicity. This phase 2b-3 trial showed that further vaccinations were halted due to lack of efficacy in preventing HIV-1 infection.
NEW ENGLAND JOURNAL OF MEDICINE
(2021)
Article
Engineering, Biomedical
Zifu Zhong, Yong Chen, Kim Deswarte, Heleen Lauwers, Emily De Lombaerde, Xiaole Cui, Simon Van Herck, Tingting Ye, Mark Gontsarik, Stefan Lienenklaus, Niek N. Sanders, Bart N. Lambrecht, Stefaan De Koker, Bruno G. De Geest
Summary: Synthetic CpG oligodeoxynucleotides can activate innate immune cells, and when combined with ionizable cationic lipid lipid nanoparticles, can trigger strong immune activation in lymph nodes, showing potential in vaccine design and cancer immunotherapy.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Biotechnology & Applied Microbiology
Qizhong Lu, Zongliang Zhang, Hexian Li, Kunhong Zhong, Qin Zhao, Zeng Wang, Zhiguo Wu, Donghui Yang, Shuang Sun, Nian Yang, Meijun Zheng, Qiang Chen, Cheng Long, Wenhao Guo, Hui Yang, Chunlai Nie, Aiping Tong
Summary: This study identified four specific nanobodies with antiviral activity against SARS-CoV-2 spike protein, with the heterodimer nanobody Nb91-Nb3-hFc showing the strongest RBD-binding affinity and neutralizing ability. The use of a naive VHH library could be a potential resource for rapid acquisition of antiviral nanobodies, with Nb91-Nb3-hFc showing potential as a therapeutic agent for COVID-19 treatment.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Cindy X. Cai, Nicole A. Doria-Rose, Nicole A. Schneck, Vera B. Ivleva, Brad Tippett, William R. Shadrick, Sarah O'Connell, Jonathan W. Cooper, Zachary Schneiderman, Baoshan Zhang, Daniel B. Gowetski, Daniel Blackstock, Jacob Demirji, Bob C. Lin, Jason Gorman, Tracy Liu, Yile Li, Adrian B. McDermott, Peter D. Kwong, Kevin Carlton, Jason G. Gall, Q. Paula Lei
Summary: This study explores the potential of CAP256V2LS as a drug for HIV-1 prevention. The researchers developed a method to separate and quantify different sulfation proteoforms and found that the fully sulfated proteoform demonstrated the highest antigen binding and neutralization efficiency. The study also highlighted the variable levels of sulfation produced by different cell lines, which can aid in the development of more potent clinical products.
SCIENTIFIC REPORTS
(2022)
Review
Biochemistry & Molecular Biology
Cathrine Scheepers, Simone I. Richardson, Thandeka Moyo-Gwete, Penny L. Moore
Summary: In this review, the significance of different antibody isotypes for HIV-1 neutralization breadth and potency is discussed, along with how this knowledge can be utilized to enhance passive and active immunization against HIV-1.
TRENDS IN MOLECULAR MEDICINE
(2022)
Article
Cell Biology
Manukumar Honnayakanahalli Marichannegowda, Michael Mengual, Amit Kumar, Elena E. Giorgi, Joshua J. Tu, David R. Martinez, Ethan O. Romero-Severson, Xiaojun Li, Liping Feng, Sallie R. Permar, Feng Gao
Summary: Research on HIV-1 evolution and selection pressure in fetuses can help understand the impact of immune responses on virus evolution and vertical transmission. It was found that most infections occur within 2 months of childbirth and fetal viruses have unique selection sites in regions targeted by antibodies and T cell responses. This suggests that the fetal immune system plays a role in shaping viral evolution.
CELL REPORTS MEDICINE
(2021)
Article
Cell Biology
Angela R. Corrigan, Hongying Duan, Cheng Cheng, Christopher A. Gonelli, Li Ou, Kai Xu, Megan E. DeMouth, Hui Geng, Sandeep Narpala, Sarah O'Connell, Baoshan Zhang, Tongqing Zhou, Manjula Basappa, Jeffrey C. Boyington, Steven J. Chen, Sijy O'Dell, Amarendra Pegu, Tyler Stephens, Yaroslav Tsybovsky, Jelle van Schooten, John P. Todd, Shuishu Wang, Nicole A. Doria-Rose, Kathryn E. Foulds, Richard A. Koup, Adrian B. McDermott, Marit J. van Gils, Peter D. Kwong, John R. Mascola
Summary: The study found that priming with FP-conjugates, either alone or as part of a cocktail with SOSIP trimers, can reduce trimer-base responses and improve neutralization outcomes compared to priming with only trimers or a mixture of SOSIP trimer and FP conjugates.
Article
Cell Biology
Marcos V. P. Gondim, Scott Sherrill-Mix, Frederic Bibollet-Ruche, Ronnie M. Russell, Stephanie Trimboli, Andrew G. Smith, Yingying Li, Weimin Liu, Alexa N. Avitto, Julia C. DeVoto, Jesse Connell, Angharad E. Fenton-May, Pierre Pellegrino, Ian Williams, Emmanouil Papasavvas, Julio C. C. Lorenzi, D. Brenda Salantes, Felicity Mampe, M. Alexandra Monroy, Yehuda Z. Cohen, Sonya Heath, Michael S. Saag, Luis J. Montaner, Ronald G. Collman, Janet M. Siliciano, Robert F. Siliciano, Lindsey J. Plenderleith, Paul M. Sharp, Marina Caskey, Michel C. Nussenzweig, George M. Shaw, Persephone Borrow, Katharine J. Bar, Beatrice H. Hahn
Summary: Type 1 interferons (IFN-I) have potent antiviral effects against HIV-1, but their resistance in later stages of infection remains high. A study of 500 clonally derived HIV-1 isolates found that resistance to IFN-I decreases in the first year after infection, increases with CD4(+) T cell loss, and is highest during viral rebound after treatment interruption. This dynamic interplay between host innate responses and the evolving HIV-1 quasispecies affects the control of HIV-1 by IFN-I.
SCIENCE TRANSLATIONAL MEDICINE
(2021)
Article
Immunology
Mina C. Hosseinipour, Craig Innes, Sarita Naidoo, Philipp Mann, Julia Hutter, Gita Ramjee, Modulakgotla Sebe, Lucas Maganga, Michael E. Herce, Allan C. DeCamp, Kyle Marshall, One Dintwe, Erica Andersen-Nissen, Georgia D. Tomaras, Nonhlanhla Mkhize, Lynn Morris, Ryan Jensen, Maurine D. Miner, Giuseppe Pantaleo, Song Ding, Olivier Van der Meeren, Susan W. Barnett, M. Juliana McElrath, Lawrence Corey, James G. Kublin
Summary: The study compared different HIV vaccine administration regimens, with the DNA and protein coadministration regimen showing better results in increasing HIV-1 V1/V2 antibody response rate, and DNA administration by Biojector leading to higher CD4(+) T-cell response rates to HIV envelope protein. Both regimens were found to be safe and promising for advancement into efficacy trials.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Immunology
Victor Raul Gomez Roman, Kristoffer Jarlov Jensen, Sanne Skov Jensen, Christian Leo-Hansen, Sanne Jespersen, David da Silva Te, Candida Medina Rodrigues, Christoph Mikkel Janitzek, Lasse Vinner, Terese Lea Katzenstein, Peter Andersen, Ingrid Kromann, Lars Vibe Andreasen, Ingrid Karlsson, Anders Fomsgaard
AIDS RESEARCH AND HUMAN RETROVIRUSES
(2013)
Letter
Immunology
Kristoffer Jarlov Jensen, Victor Raul Gomez Roman, Sanne Skov Jensen, Christian Leo-Hansen, Ingrid Karlsson, Terese Lea Katzenstein, Candida Medina Rodrigues, Sanne Jespersen, Christoph Mikkel Janitzek, David da Silva Te, Peter Hayes, Anders Fomsgaard
CLINICAL AND VACCINE IMMUNOLOGY
(2012)
Article
Immunology
Victor Raul Gomez Roman, Sanne Skov Jensen, Christian Leo-Hansen, Kristoffer Jarlov Jensen, Christoph Mikkel Janitzek, Candida Medina Rodrigues, Sanne Jespersen, Terese Lea Katzenstein, David da Silva Te, Anders Fomsgaard
CLINICAL AND VACCINE IMMUNOLOGY
(2012)
Article
Biochemical Research Methods
Victor Raul Gomez Roman, Lasse Vinner, Berit Grevstad, Jesper Juhl Hansen, Frank Wegmann, Anna-Lena Spetz, Anders Fomsgaard
JOURNAL OF IMMUNOLOGICAL METHODS
(2010)
Article
Biochemical Research Methods
Harry Kaltsidis, Hannah Cheeseman, Jakub Kopycinski, Ambreen Ashraf, Michelle Cashin Cox, Lorna Clark, Insiyah Anjarwalla, Len Dally, Phillip Bergin, Aggeliki Spentzou, Chris Higgs, Frances Gotch, Brian Gazzard, Raul Gomez, Peter Hayes, Peter Kelleher, Dilbinder K. Gill, Jill Gilmour
JOURNAL OF IMMUNOLOGICAL METHODS
(2011)
Review
Immunology
P. Pala, V. R. Gomez-Roman, J. Gilmour, P. Kaleebu
MUCOSAL IMMUNOLOGY
(2009)
Article
Public, Environmental & Occupational Health
Valentina Bernasconi, Paul A. Kristiansen, Mike Whelan, Raid Gomez Roman, Alison Bettis, Solomon Abebe Yimer, Celine Gurry, Svein R. Andersen, Debra Yeskey, Henshaw Mandi, Arun Kumar, Johan Holst, Carolyn Clark, Jakob P. Cramer, John-Arne Rottingen, Richard Hattchet, Melanie Saville, Gunnstein Norheim
BUNDESGESUNDHEITSBLATT-GESUNDHEITSFORSCHUNG-GESUNDHEITSSCHUTZ
(2020)
Editorial Material
Biotechnology & Applied Microbiology
Tung Thanh Le, Zacharias Andreadakis, Arun Kumar, Raul Gomez Roman, Stig Tollefsen, Melanie Saville, Stephen Mayhew
NATURE REVIEWS DRUG DISCOVERY
(2020)
Editorial Material
Microbiology
Raul Gomez Roman, Lin-Fa Wang, Benhur Lee, Kim Halpin, Emmie de Wit, Christopher C. Broder, Mahmudur Rahman, Paul Kristiansen, Melanie Saville
Review
Infectious Diseases
Arun Kumar, William E. Dowling, Raul Gomez Roman, Amol Chaudhari, Celine Gurry, Tung Thanh Le, Stig Tollefson, Carolyn E. Clark, Valentina Bernasconi, Paul A. Kristiansen
Summary: This review discusses the development of COVID-19 vaccines, including various types and efficacy. Recent findings of new variants suggest that current vaccines may need updates to combat these new strains.
CURRENT INFECTIOUS DISEASE REPORTS
(2021)
Review
Infectious Diseases
Raul Gomez Roman, Nadia Tornieporth, Neil George Cherian, Amy C. Shurtleff, Maina L'Azou Jackson, Debra Yeskey, Adam Hacker, Eric Mungai, Tung Thanh Le
Summary: Henipaviruses, including Nipah virus, are considered pathogens with notable epidemic potential due to their high pathogenicity and the lack of specific medical countermeasures. Innovative strategies are necessary to address the sporadic and unpredictable nature of henipavirus outbreaks. Stronger partnerships with scientific institutions and regulatory authorities in low-income and middle-income countries are essential to inform appropriate investments and development of strategies for the equitable use of multiple medical countermeasures.
LANCET INFECTIOUS DISEASES
(2022)
Article
Immunology
Qifeng Zhou, Rachmat Hidajat, Bo Peng, David Venzon, M. Kristine Aldrich, Ersell Richardson, Eun Mi Lee, V. S. Kalyanaraman, George Grimes, V. Raul Gomez-Roman, L. Ebonita Summers, Nina Malkevich, Marjorie Robert-Guroff
Article
Immunology
V. Paul Gomez-Roman, Ruth H. Florese, Bo Peng, David C. Montefiori, Vaniambadi S. Kalyanaraman, David Venzon, Indresh Srivastava, Susan W. Barnett, Marjorie Robert-Guroff
JAIDS-JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES
(2006)
Article
Immunology
V. Raul Gomez-Roman, George J. Grimes, Gopal K. Potti, Bo Peng, Thorsten Demberg, Luisa Gravlin, James Treece, Ranajit Pal, Eun Mi Lee, W. Gregory Alvord, Phillip D. Markham, Marjorie Robert-Guroff
Article
Virology
Naiqing Xu, Xinen Tang, Xin Wang, Miao Cai, Xiaowen Liu, Xiaolong Lu, Shunlin Hu, Min Gu, Jiao Hu, Ruyi Gao, Kaituo Liu, Yu Chen, Xiufan Liu, Xiaoquan Wang
Summary: This study found that the H9N2 subtype avian influenza virus has a high airborne transmissibility, while the H7N9 virus does not. The Hemagglutinin protein of the H9N2 virus was found to play a key role in replication, stability, and airborne transmission.
Article
Virology
Samar S. Ewies, Sabry M. Tamam, Ahmed S. Abdel-Moneim, Sherin R. Rouby
Summary: Contagious ecthyma (CE) is a highly contagious viral disease of sheep and goats worldwide. The study provided a clinical description of CE and screened for genetic variation in the B2L gene. Infected sheep exhibited anorexia and oral lesions, while inoculated chicken embryos showed pock lesions. The B2L gene was successfully amplified and found to be highly conserved.
Article
Virology
Yigal Farnoushi, Dan Heller, Avishai Lublin
Summary: In recent years, new variants of avian reovirus (ARV) have caused a variety of symptoms in chickens worldwide, including viral arthritis/tenosynovitis. This study analyzed emerging ARV variants in Israel and found significant genetic diversity. Most ARV isolates in Israel belonged to genotypic cluster 5 (GC5). The study suggests that Israel has not experienced the emergence of new ARV variants since the introduction of the live vaccine (ISR-7585), but ongoing monitoring is needed due to the continuous emergence of ARV variants.
Article
Virology
Shigeru Tajima, Michiyo Kataoka, Yuki Takamatsu, Hideki Ebihara, Chang-Kweng Lim
Summary: Yokose virus (YOKV), a bat-associated flavivirus, was found to replicate at a slower rate in mosquito cells compared to other mosquito-borne flaviviruses. Specific nucleotide mutations in the virus were identified to enhance its proliferation ability in mosquito cells.
Article
Virology
Alejandra Borjabad, Baojun Dong, Wei Chao, David J. Volsky, Mary Jane Potash
Summary: This study investigated HIV brain disease using a mouse model, and found that poly I:C can reverse associated cognitive impairment and reduce virus burden. The results also revealed transcriptional changes related to neuronal function and innate immune responses.
Article
Virology
Ching-Hung Lin, Feng-Cheng Hsieh, Meilin Wang, Chieh Hsu, Hsuan-Wei Hsu, Chun-Chun Yang, Cheng-Yao Yang, Hung-Yi Wu
Summary: This study demonstrates that the synthesis of coronavirus subgenomic mRNA is not solely determined by the sequence homology between the leader TRS and TRS-B, but also by the disassociation of the coronavirus polymerase from the viral genome. This finding provides a new insight into the transcription mechanism of coronaviruses.
Article
Virology
Nicholas S. Kron, Benjamin W. Neuman, Sathish Kumar, Patricia L. Blackwelder, Dayana Vidal, Delphina Z. Walker-Phelan, Patrick D. I. Gibbs, Lynne A. Fieber, Michael C. Schmale
Summary: Two recent studies documented the genome of a novel virus in marine animals, finding that the virus is widespread in apparently healthy animals but not highly expressed in neurons. The studies also identified viral replication factories and high levels of defective genomes in chronically infected animals.
Article
Virology
Andrew M. Ramey, Laura C. Scott, Christina A. Ahlstrom, Evan J. Buck, Alison R. Williams, Mia Kim Torchetti, David E. Stallknecht, Rebecca L. Poulson
Summary: We successfully detected and characterized highly pathogenic avian influenza viruses in hunter-harvested wild waterfowl samples from western Alaska. Genomic analysis revealed three independent viral introductions into Alaska. Our findings demonstrate the utility and potential limitations of using molecular processing approaches directly on original swab samples for viral research and monitoring.
Article
Virology
Ting Gong, Dongdong Wu, Yongzhi Feng, Xing Liu, Qi Gao, Xiaoyu Zheng, Zebu Song, Heng Wang, Guihong Zhang, Lang Gong
Summary: This study discovered that quercetin can inhibit PEDV replication both in vivo and in vitro, and alleviate the clinical symptoms and intestinal injury caused by the virus. This provides a new direction for the development of PED antiviral drugs.
Article
Virology
Min Zhu, Hao Zeng, Jianqiao He, Yaohui Zhu, Pingping Wang, Jianing Guo, Jinfan Guo, Huabo Zhou, Yifeng Qin, Kang Ouyang, Zuzhang Wei, Weijian Huang, Ying Chen
Summary: The reassortment between avian H9N2 and Eurasian avian-like (EA) H1N1 viruses may have potentially changed from avian-to-mammals adaptation. This study found that the introduction of EA H1N1 internal genes into H9N2 virus restored the replication capability and resulted in extreme virulence in some cases. This raises new concerns for public health due to the possible coexistence of H9N2 and EA H1N1 viruses in dogs.
