期刊
VIROLOGY
卷 373, 期 1, 页码 98-111出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2007.11.012
关键词
herpes simplex virus; HSV; entry; nectin-1; glycoprotein; down-regulation; endocytosis; receptor
类别
资金
- NIAID NIH HHS [R37 AI018289, R21 AI056045, R01 AI076231, R01 AI056045-04, R37 AI018289-26, AI-18289, AI-056045, R01 AI018289, R01 AI056045] Funding Source: Medline
- NIGMS NIH HHS [T32 GM007229-32, T32 GM007229, T32-GM07229] Funding Source: Medline
- NINDS NIH HHS [R01 NS036731, NS-36731, R01 NS036731-10] Funding Source: Medline
During herpes simplex virus (HSV) entry, membrane fusion occurs either on the cell surface or after virus endocytosis. In both cases, binding of glycoprotein D (gD) to a receptor such as nectin-1 or HVEM is required. In this study, we co-cultured cells expressing gD with nectin-1 expressing cells to investigate the effects of gD on nectin-1 at cell contacts. After overnight co-cultures with gD expressing cells, there was a down-regulation of nectin-1 in B78H1-C10, SY5Y, A431 and HeLa cells, which HSV enters by endocytosis. In contrast, on Vero cells, which HSV enters at the plasma membrane, nectin-1 was not down-regulated. Further analysis of B78H1-derived cells showed that nectin-1 downregulation corresponds to the ability of gD to bind nectin-1 and is achieved by internalization and low-pH-dependent degradation of nectin-1. Moreover, gD is necessary for virion internalization in B78H1 cells expressing nectin-1. These data suggest that the determinants of gD-mediated internalization of nectin-1 may direct HSV to an endocytic pathway during entry. (C) 2007 Elsevier Inc. All rights reserved.
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