期刊
VIROLOGY
卷 382, 期 2, 页码 182-189出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2008.09.012
关键词
Immunology; Protein expression; Torque teno virus; TTV; Anellovirus
类别
资金
- Helsinki Biomedical Graduate School
- Biomedicum Helsinki Foundation
- Alfred Kordelin Foundation
- Research arid Science Foundation of Farmos
- Academy of Finland [122539]
- Finnish Konkordia Fund
- Ella and Georg Ehrnrooth Foundation
- Paulo Foundation
- Finnish Foundation for Research on Viral Diseases
- Medical Society of Finland (FLS)
- Finnish Funding Agency for Technology and Innovation (Tekes)
- Helsinki University Central Hospital Research and Education Fund
- Sigrid Juselius Foundation
- Maud Kuistila Memorial Foundation
- Academy of Finland (AKA) [122539, 122539] Funding Source: Academy of Finland (AKA)
Torque teno virus (TTV) is a non-enveloped human virus with a circular (similar to 3800 nt) ssDNA genome. TTV transcription results in three Viral mRNAs and six proteins, the function or antigenicity of which are unknown. The six open reading frames of TTV genotype 6 were expressed in bacteria and insect cells. Expression of the ORF1/1-encoded protein was inefficient, while expression of the others was Successful, with ORFI and ORF1/2 as arginine-rich region depleted. All six recombinant TTV proteins were antigenic. of healthy adults, 11/25 (44%) showed strong IgG reactivity with one or more proteins. Four Subjects, two Of Whom Were genotype-6-DNA positive, were followed. One of the latter showed concurrently a strong IgG response against the ORF1 protein. The other showed appearance of IgG against the ORF2 protein concomitantly with resolution of the genotype-6 viremia. The genotype-6 sequences remained Unaltered for years, suggesting that some mechanisms other than amino acid substitutions play a role in TTV immune evasion. (C) 2008 Elsevier Inc. All rights reserved
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