Article
Biotechnology & Applied Microbiology
Jesus Lavado-Garcia, Inmaculada Jorge, Arnau Boix-Besora, Jesus Vazquez, Francesc Godia, Laura Cervera
Summary: Developing new vaccine technologies that can adapt to global virus outbreaks, such as the recent SARS-CoV-2 pandemic, is crucial. Virus-like particles (VLPs) offer a promising approach for vaccine candidates, with HIV-1 Gag VLPs being a flexible system that has shown success against various diseases. The study established an analytical method to characterize the Gag protein core and determine the variability of Gag stoichiometry in HIV-1 VLPs, providing a key advantage in quantifying and ensuring the quality of VLP production on a large scale for new recombinant vaccine production technologies.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Review
Biochemistry & Molecular Biology
Veronna Marie, Michelle Lucille Gordon
Summary: The HIV-1 Gag polyprotein, originally believed to only contribute to the physical nature of the virus, has been found to have multiple roles in viral replication and functionality. It can mediate its own trafficking, interact with host factors, and aid in viral genome packaging. It has also been associated with drug resistance and treatment failure.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Yi-Ru Lin, Shih-Ming Chu, Fu-Hsien Yu, Kuo-Jung Huang, Chin-Tien Wang
Summary: The amount of HIV-1 Gag-Pol or Pol viral incorporation is largely dependent on virus particle production, and cleavage blocking in the Gag-Pol N-terminal Gag domain does not exert significant impacts on Pol packaging.
Article
Microbiology
Denise Jurczyszak, Lara Manganaro, Sofija Buta, Conor Gruber, Marta Martin-Fernandez, Justin Taft, Roosheel Patel, Melissa Cipolla, Hala Alshammary, Lubbertus C. F. Mulder, Ravi Sachidanandam, Dusan Bogunovic, Viviana Simon
Summary: The study demonstrates that ISG15-deficient cells have superior resistance to HIV-1 infection. Gene expression analysis shows that ISG15-knockout CD4(+) T cells express similar inflammatory markers as ISG15-deficient patients.
Article
Biochemistry & Molecular Biology
Hitoshi Sawada, Shukumi Inoue, Takako Saito, Kei Otsuka, Maki Shirae-Kurabayashi
Summary: The extracellular ubiquitin-proteasome system plays a role in sperm binding to and penetration of the vitelline coat during fertilization in ascidian Halocynthia roretzi. The inhibitor of UBA strongly inhibits the fertilization process in H. roretzi. The UBA1 and UBA6 genes are found to be expressed in the ovary and testis and may be involved in the ubiquitination of HrVC70.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Abdul A. Waheed, Yanan Zhu, Eva Agostino, Lwar Naing, Yuta Hikichi, Ferri Soheilian, Seung- Wan Yoo, Yun Song, Peijun Zhang, Barbara S. Slusher, Norman J. Haughey, Eric O. Freed
Summary: This study demonstrates the essential role of nSMase2 in the morphogenesis and maturation of HIV-1 particles, as well as other primate lentiviruses. Inhibition or depletion of nSMase2 results in the production of immature and non-infectious viral particles.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
Alexandra B. Samal, Todd J. Green, Jamil S. Saad
Summary: This study reveals the detailed structure of the myrMA lattice and its role in Env incorporation during HIV-1 particle assembly. It was found that substitution of MA residues Leu13 and Leu31 induced a conformational change in myrMA, which may destabilize the trimer-trimer interactions within the lattice.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Biochemistry & Molecular Biology
Charlotte Bussienne, Roland Marquet, Jean-Christophe Paillart, Serena Bernacchi
Summary: Protein post-translational modifications (PTMs) play key roles in regulating the functions and replication of HIV-1 Pr55(Gag), affecting its localization and infection mechanisms. These modifications also facilitate viral uncoating and assembly by allowing the virus to hijack cellular machinery.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Vyacheslav Akimov, Mirjam Fehling-Kaschek, Inigo Barrio-Hernandez, Michele Puglia, Jakob Bunkenborg, Mogens M. Nielsen, Jens Timmer, Joern Dengjel, Blagoy Blagoev
Summary: Receptor tyrosine kinases (RTK) play crucial roles in cell proliferation and differentiation by binding growth factors, with EGFR being a prototypic RTK that can bind multiple ligands. A proteomics-based workflow combined with mathematical modeling revealed that ligand-specific ubiquitination events on EGFR play a significant role in signal attenuation and termination. The absolute magnitude of EGFR ubiquitination, rather than distinctly regulated ubiquitination sites, is a major determinant for signal attenuation and subsequent cellular outcomes.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Medicine, Research & Experimental
Suzanne L. Campion, Elena Brenna, Elaine Thomson, Will Fischer, Kristin Ladell, James E. McLaren, David A. Price, Nicole Frahm, Juliana M. McElrath, Kristen W. Cohen, Janine R. Maenza, Stephen R. Walsh, Lindsey R. Baden, Barton F. Haynes, Bette Korber, Persephone Borrow, Andrew J. McMichael
Summary: A study found that preexisting memory CD4(+) T cells could shape the early immune response to vaccination with a previously unencountered HIV-1 antigen in HIV-1-seronegative volunteers who received an HIV-1 vaccine.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Biochemistry & Molecular Biology
Yingke Tang, Ryan T. Behrens, Corine St Gelais, Siqi Wu, Saravanan Vivekanandan, Ehud Razin, Pengfei Fang, Li Wu, Nathan Sherer, Karin Musier-Forsyth
Summary: Human lysyl-tRNA synthetase (LysRS) is re-localized from cytoplasm to nucleus in HIV-1 infected cells through phosphorylation. Phosphorylation promotes HIV-1 transcription, and nuclear pS207-LysRS generates Ap4A, which also activates HIV-1 transcription. Additionally, MSC-derived peptide stabilizes LysRS MSC binding and inhibits HIV-1 replication.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Microbiology
Jerome Kervevan, Aurelie Bouteau, Juliane S. Lanza, Adele Hammoudi, Sandra Zurawski, Mathieu Surenaud, Lydie Dieudonne, Marion Bonnet, Cecile Lefebvre, Hakim Hocini, Romain Marlin, Aurelie Guguin, Barbara Hersant, Oana Hermeziu, Elisabeth Menu, Christine Lacabaratz, Jean-Daniel Lelievre, Gerard Zurawski, Veronique Godot, Sandrine Henri, Botond Z. Igyarto, Yves Levy, Sylvain Cardinaud
Summary: Targeting of skin Langerhans cells (LC) may efficiently induce potent anti-HIV immune responses, serving as a promising strategy for developing effective HIV-1 vaccines. This approach demonstrates the potential of directing immune responses through specific cell targeting and may lead to the induction of protective antibodies against HIV-1.
Article
Immunology
Elisa Saccon, Flora Mikaeloff, Pol Figueras Ivern, Akos Vegvari, Anders Sonnerborg, Ujjwal Neogi, Robert van Domselaar
Summary: Untreated HIV-1 infection leads to a decrease in CD4(+) T cell lymphocytes, susceptibility to opportunistic infections, and ultimately death. This study found that cytotoxic lymphocytes can target the HIV-1 Gag protein through granzyme cleavage to potentially control HIV-1 infection.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Virology
Jinwei Zhang
Summary: The cellular metabolism of host tRNAs and the life cycle of HIV-1 intersect at various key interfaces, with the virus hijacking host tRNAs for reverse transcription and structural protein regulation, providing potential targets for the development of HIV/AIDS treatments.
Review
Immunology
Fabien Thery, Denzel Eggermont, Francis Impens
Summary: ISG15 is an important antimicrobial protein that acts through the ISGylation process, but its substrate repertoire remains unclear. Mass spectrometry-based proteomics studies can reveal the mechanism of action and substrates of ISG15.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Endocrinology & Metabolism
Corinne Caillaud, Mie Mechta, Heidi Ainge, Andreas N. Madsen, Patricia Ruell, Emilie Mas, Catherine Bisbal, Jacques Mercier, Stephen Twigg, Trevor A. Mori, David Simar, Romain Barres
JOURNAL OF ENDOCRINOLOGY
(2015)
Article
Nutrition & Dietetics
Karen Lambert, Marjorie Coisy-Quivy, Catherine Bisbal, Pascal Sirvent, Gerald Hugon, Jacques Mercier, Antoine Avignon, Ariane Sultan
Article
Endocrinology & Metabolism
Cyril Breuker, Cacylde Amouzou, Odile Fabre, Karen Lambert, Pascal Seyer, Annick Bourret, Tamim Salehzada, Jacques Mercier, Ariane Sultan, Catherine Bisbal
METABOLISM-CLINICAL AND EXPERIMENTAL
(2018)
Article
Cell Biology
Karen Lambert, Marie Hokayem, Claire Thomas, Odile Fabre, Cecile Cassan, Annick Bourret, Florence Bernex, Jessica Lees, Marie Demion, Pascal Seyer, Gerald Hugon, Jacques Mercier, Antoine Avignon, Catherine Bisbal
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2018)
Article
Multidisciplinary Sciences
Karen Lambert, Marie Hokayem, Claire Thomas, Odile Fabre, Cecile Cassan, Annick Bourret, Florence Bernex, Christine Feuillet-Coudray, Cecile Notarnicola, Jacques Mercier, Antoine Avignon, Catherine Bisbal
SCIENTIFIC REPORTS
(2018)
Article
Multidisciplinary Sciences
Cacylde Amouzou, Cyril Breuker, Odile Fabre, Annick Bourret, Karen Lambert, Olivier Birot, Christine Fedou, Anne-Marie Dupuy, Jean-Paul Cristol, Thibault Sutra, Nicolas Molinari, Laurent Maimoun, Denis Mariano-Goulart, Florence Galtier, Antoine Avignon, Francoise Stanke-Labesque, Jacques Mercier, Ariane Sultan, Catherine Bisbal
Article
Cell Biology
O. Fabre, C. Breuker, C. Amouzou, T. Salehzada, M. Kitzmann, J. Mercier, C. Bisbal
CELL DEATH & DISEASE
(2014)
Article
Virology
Michele Bengue, Pauline Ferraris, Cecile Baronti, Cheikh Tidiane Diagne, Loic Talignani, Sineewanlaya Wichit, Florian Liegeois, Catherine Bisbal, Antoine Nougairede, Dorothee Misse
Article
Biochemistry & Molecular Biology
Karen Lambert, Marie Demion, Jean-Christophe Lagace, Marie Hokayem, Mamta Dass, Anne Virsolvy, Bernard Jover, Annick Bourret, Catherine Bisbal
Summary: The study found that exercise and polyphenols can prevent cardiac hypertrophy, but their combination does not bring additional benefits. Rats supplemented with polyphenols showed a shift from pathologic to physiological hypertrophy. Polyphenols supplementation appears to have a better protective effect in sedentary obese insulin-resistant rats compared to exercised rats.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2021)
Editorial Material
Physiology
Luc Pellerin, Philippe Connes, Catherine Bisbal, Karen Lambert
FRONTIERS IN PHYSIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Adriana Capozzi, Cedric Saucier, Catherine Bisbal, Karen Lambert
Summary: Inactivity and high-calorie diets contribute to obesity and premature aging, while improvements in healthcare management have led to a growing population of elderly people. Obesity and aging are major risk factors for non-communicable diseases that result in comorbidities and increased mortality, particularly affecting skeletal muscle mass and quality. Skeletal muscles are important for various bodily functions and limiting their deterioration is a significant public health and socio-economic challenge. Supplementation with natural compounds like grape polyphenols has shown potential in regulating metabolic pathways and preventing obesity and aging-related muscle comorbidities in cell and animal studies. However, there is a lack of clinical studies validating these promising results observed in animal models.
Meeting Abstract
Physiology
Karen Lambert, Cacylde Amouzou, Annick Bourret, Cedric Moro, Odile Fabre, Jacques Mercier, Ariane Sultan, Catherine Bisbal
Meeting Abstract
Physiology
Karen Lambert, Marie Hokayem, Marie Demion, Claire Thomas, Alice Fevet, Mamta Dass, Anne Virsolvy, Bernard Jover, Annick Bourret, Jacques Mercier, Catherine Bisbal
Meeting Abstract
Endocrinology & Metabolism
C. Amouzou, C. Breuker, K. Lambert, F. Galtier, J. Cristol, A. Avignon, J. Mercier, A. Sultan, C. Bisbal
Meeting Abstract
Endocrinology & Metabolism
A. Sultan, O. Fabre, C. Amouzou, C. Breuker, K. Lambert, C. Fedou, A. Dupuis, J. Cristol, F. Galtier, A. Avignon, J. Mercier, C. Bisbal
Article
Virology
Naiqing Xu, Xinen Tang, Xin Wang, Miao Cai, Xiaowen Liu, Xiaolong Lu, Shunlin Hu, Min Gu, Jiao Hu, Ruyi Gao, Kaituo Liu, Yu Chen, Xiufan Liu, Xiaoquan Wang
Summary: This study found that the H9N2 subtype avian influenza virus has a high airborne transmissibility, while the H7N9 virus does not. The Hemagglutinin protein of the H9N2 virus was found to play a key role in replication, stability, and airborne transmission.
Article
Virology
Samar S. Ewies, Sabry M. Tamam, Ahmed S. Abdel-Moneim, Sherin R. Rouby
Summary: Contagious ecthyma (CE) is a highly contagious viral disease of sheep and goats worldwide. The study provided a clinical description of CE and screened for genetic variation in the B2L gene. Infected sheep exhibited anorexia and oral lesions, while inoculated chicken embryos showed pock lesions. The B2L gene was successfully amplified and found to be highly conserved.
Article
Virology
Yigal Farnoushi, Dan Heller, Avishai Lublin
Summary: In recent years, new variants of avian reovirus (ARV) have caused a variety of symptoms in chickens worldwide, including viral arthritis/tenosynovitis. This study analyzed emerging ARV variants in Israel and found significant genetic diversity. Most ARV isolates in Israel belonged to genotypic cluster 5 (GC5). The study suggests that Israel has not experienced the emergence of new ARV variants since the introduction of the live vaccine (ISR-7585), but ongoing monitoring is needed due to the continuous emergence of ARV variants.
Article
Virology
Shigeru Tajima, Michiyo Kataoka, Yuki Takamatsu, Hideki Ebihara, Chang-Kweng Lim
Summary: Yokose virus (YOKV), a bat-associated flavivirus, was found to replicate at a slower rate in mosquito cells compared to other mosquito-borne flaviviruses. Specific nucleotide mutations in the virus were identified to enhance its proliferation ability in mosquito cells.
Article
Virology
Alejandra Borjabad, Baojun Dong, Wei Chao, David J. Volsky, Mary Jane Potash
Summary: This study investigated HIV brain disease using a mouse model, and found that poly I:C can reverse associated cognitive impairment and reduce virus burden. The results also revealed transcriptional changes related to neuronal function and innate immune responses.
Article
Virology
Ching-Hung Lin, Feng-Cheng Hsieh, Meilin Wang, Chieh Hsu, Hsuan-Wei Hsu, Chun-Chun Yang, Cheng-Yao Yang, Hung-Yi Wu
Summary: This study demonstrates that the synthesis of coronavirus subgenomic mRNA is not solely determined by the sequence homology between the leader TRS and TRS-B, but also by the disassociation of the coronavirus polymerase from the viral genome. This finding provides a new insight into the transcription mechanism of coronaviruses.
Article
Virology
Nicholas S. Kron, Benjamin W. Neuman, Sathish Kumar, Patricia L. Blackwelder, Dayana Vidal, Delphina Z. Walker-Phelan, Patrick D. I. Gibbs, Lynne A. Fieber, Michael C. Schmale
Summary: Two recent studies documented the genome of a novel virus in marine animals, finding that the virus is widespread in apparently healthy animals but not highly expressed in neurons. The studies also identified viral replication factories and high levels of defective genomes in chronically infected animals.
Article
Virology
Andrew M. Ramey, Laura C. Scott, Christina A. Ahlstrom, Evan J. Buck, Alison R. Williams, Mia Kim Torchetti, David E. Stallknecht, Rebecca L. Poulson
Summary: We successfully detected and characterized highly pathogenic avian influenza viruses in hunter-harvested wild waterfowl samples from western Alaska. Genomic analysis revealed three independent viral introductions into Alaska. Our findings demonstrate the utility and potential limitations of using molecular processing approaches directly on original swab samples for viral research and monitoring.
Article
Virology
Ting Gong, Dongdong Wu, Yongzhi Feng, Xing Liu, Qi Gao, Xiaoyu Zheng, Zebu Song, Heng Wang, Guihong Zhang, Lang Gong
Summary: This study discovered that quercetin can inhibit PEDV replication both in vivo and in vitro, and alleviate the clinical symptoms and intestinal injury caused by the virus. This provides a new direction for the development of PED antiviral drugs.
Article
Virology
Min Zhu, Hao Zeng, Jianqiao He, Yaohui Zhu, Pingping Wang, Jianing Guo, Jinfan Guo, Huabo Zhou, Yifeng Qin, Kang Ouyang, Zuzhang Wei, Weijian Huang, Ying Chen
Summary: The reassortment between avian H9N2 and Eurasian avian-like (EA) H1N1 viruses may have potentially changed from avian-to-mammals adaptation. This study found that the introduction of EA H1N1 internal genes into H9N2 virus restored the replication capability and resulted in extreme virulence in some cases. This raises new concerns for public health due to the possible coexistence of H9N2 and EA H1N1 viruses in dogs.