pH-Induced conformational changes of BSA in fluorescent AuNCs@BSA and its effects on NCs emission

We investigated the pH-induced fluorescence changes of BSA-protected gold nanoclusters, Au16NCs@BSA, and the corresponding conformational changes of ligand protein by fluorescence, circular dichrosim (CD) and IR spectral measurements. The studies presented here demonstrated that BSA in AuNCs@BSA underwent identifiable conformational changes on both the secondary and the tertiary structure levels. The results of CD and IR interpreted the significant change of second structures at extreme acidity and alkaline, where more unordered structures were gained. Of note was that the extreme alkaline (pH = 11.43) induced the changes from exposed to buried alpha-helices, which was different from the pH-induced structural changes of BSA. In addition, the large fluorescence intensity gap of tryptophan between AuNCs@BSA and native BSA indicated efficient energy transfer took place between BSA and AuNCs, implying that the gold core resided near tryptophan in BSA. (c) 2013 Elsevier B.V. All rights reserved.