Article
Environmental Sciences
Zihan Xu, Jing Liu, Ermeng Wang, Chenyun Zhao, Xuelei Hu, Ka Hou Chu, Lan Wang
Summary: This study found that the freshwater crab Sinopotamon henanense responded to cadmium exposure with changes in microstructure, biomacromolecules, and biochemical parameters in the gills and hepatopancreas. Antioxidant defense mechanisms were activated, oxidative damage increased, and neurotoxicity was inhibited as cadmium bioaccumulated. During depuration, a significant portion of cadmium was excreted, leading to recovery of microstructure and biomacromolecules in the organs. Optimal biomarkers for indicating crab health status were identified. Overall, S. henanense showed resistance to cadmium stress through antioxidant defense and self-detoxification mechanisms.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Environmental Sciences
Ruijing Xuan, Hao Wu, Yingjun Li, Bingyan Wei, Lan Wang
Summary: Studies on the freshwater crab Sinopotamon henanense have shown that acute and sub-chronic exposure to Cd2+ can induce differential alterations in respiratory physiology and gill morphology. The research revealed that sub-chronic exposure resulted in lower MT content and higher MDA levels in the gills, while acute exposure decreased PPP activity and NADPH content in the hepatopancreas. Morphological changes in the sinus gland were observed in crabs exposed to 2.86 mg/L Cd2+ for 3 weeks, providing evidence for S. henanense as a biomarker of acute or long-term waterborne Cd2+ pollution.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Fisheries
Yue Liang, Minnan Bao, Lang Lang, Lu Wang, Shijie Wang, Chien M. Chen, Ka-Hou Chu, Lan Wang
Summary: This study investigated the function and expression changes of proPO in freshwater crab Sinopotamon henanense (ShproPO) in response to both cadmium (Cd) and Aeromonas hydrophila infection. ShproPO was found to be involved in the antibacterial process and its expression was promoted by low concentrations of Cd after A. hydrophila infection. Additionally, ShproPO was identified as a key enzyme in melanization response and was found to enhance phagocytosis of A. hydrophila-infected hemocytes.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Environmental Sciences
Zhi-wen Sang, Min-nan Bao, Yue Liang, Ka-hou Chu, Lan Wang
Summary: In this study, the ACP gene of the freshwater crab Sinopotamon henanense was cloned and the impact of cadmium stress on the expression and function of ShACP was investigated. The findings revealed that ShACP enhances immune function, but high levels of cadmium stress inhibit its expression. This study provides a theoretical foundation for further research on crab immune systems and has significant implications for studying immune responses in crustaceans under heavy metal stress.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Environmental Sciences
Jing Liu, Ermeng Wang, Ziru Cheng, Yuan Gao, Chienmin Chen, Ru Jia, Zhi Luo, Lan Wang
Summary: Cadmium (Cd) is a carcinogenic environmental pollutant that harms male reproductive systems, but the mitigating effects of zinc (Zn) on Cd-induced toxicity have not been fully elucidated. This study investigated the effects of Zn supplementation on Cd-exposed freshwater crabs. Cd exposure caused Cd accumulation, Zn deficiency, decreased sperm survival rate, poor sperm quality, altered ultrastructure, increased apoptosis, and elevated metallothionein (MT) expression in the testis. However, Zn supplementation effectively alleviated these effects by preventing Cd accumulation, increasing Zn bioavailability, alleviating apoptosis, decreasing reactive oxygen species (ROS) levels, and restoring MT distribution. Furthermore, Zn also regulated the expression of apoptosis-related genes and proteins, metal transporter-related genes, and MT expression. These findings provide important insights for the development of mitigation strategies for Cd contamination or poisoning.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Fisheries
Minnan Bao, Yue Liang, Lang Lang, Chien Min Chen, Zuobing Zhang, Lan Wang
Summary: This study reports a novel antimicrobial peptide, Shcrustin, from the freshwater crab Sinopotamon henanense, which exhibits inhibitory activity against gram-positive and gram-negative bacteria. Shcrustin is expressed in different tissues of the crab and its expression increases after bacterial challenge but is suppressed in the presence of cadmium. RNA interference experiments suggest that Shcrustin may be involved in hemolymph phagocytosis to clear invading pathogens. Therefore, Shcrustin plays an important role in innate immunity and its expression and immune function may be affected by cadmium.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Environmental Sciences
Maria Hernandez-Lopez, Diego Romero
Summary: Aquatic ecosystems are threatened by pollutants, including plastics and toxic elements. This study evaluated the accumulation and distribution of cadmium in blue crab tissues exposed to microplastics. The presence of microplastics did not affect cadmium concentrations in the crabs, except for oxidized microplastics where a positive correlation was found between cadmium concentrations in the hepatopancreas and muscles, which is relevant for food safety.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Environmental Sciences
Min Sun, Jun Qing Liu, Xiao Lin Du, Si Qi Liu, Lan Wang
Summary: Cadmium exposure disrupts the expression and function of Shvasa, as well as the functions of Shnanos and Shpiwi, leading to severe reproductive toxicity in Sinopotamon henanense.
Article
Environmental Sciences
Y. Y. Wu, W. F. Tian, C. X. Cheng, L. Yang, Q. Q. Ye, W. H. Li, J. Y. Jiang
Summary: This study investigated the toxic effects of cadmium on the freshwater snail Cipangopaludina cathayensis. The results showed that cadmium exposure led to metabolic disorders, oxidative stress, and immunosuppression. Transcriptome analysis identified differentially expressed genes related to energy metabolism, antioxidant activity, and immune function. These findings suggest that cadmium exposure may contribute to disease outbreaks in freshwater snails.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Biochemistry & Molecular Biology
Ermeng Wang, Jing Liu, Chenyun Zhao, Yuan Gao, Ziru Cheng, Chien-Min Chen, Lan Wang
Summary: In this study, the genetic information of Pgp in the freshwater crab Sinopotamon henanense (ShPgp) was revealed for the first time. It was found that ShPgp was widely expressed in various tissues and mainly distributed in the cytoplasm and cell membrane. Exposure to cadmium or cadmium-containing quantum dots (Cd-QDs) resulted in increased expression of ShPgp mRNA and protein, as well as enhanced multi-xenobiotic resistance (MXR) activity and ATP contents. Knockdown of Shpgp led to elevated apoptosis and altered gene expression in the crabs. MTF1 and HSF1 were identified as regulators of ShPgp, while PPAR had limited regulatory effect.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Ecology
Yanjun Shen, Qinghua Li, Ruli Cheng, Yang Luo, Yufeng Zhang, Qing Zuo
Summary: In this study, the complete mitogenomes of Sinopotamon chishuiense and the nearly complete mitogenomes of S. wushanense were sequenced and analyzed. Novel patterns of gene rearrangement were detected, and the phylogenetic relationship was revealed. The results have significant implications for understanding the evolutionary history and taxonomic relationship of Sinopotamon.
ECOLOGY AND EVOLUTION
(2023)
Article
Physiology
Yingjiang Xu, Huan Liu, Dianfeng Han, Lihua Ren, Xianghong Gong, Fang Jiang, Yanmei Cui, Xiaojing Liu, Chuanbo Ren, Jinglin Xue, Xiuhui Tian
Summary: The study revealed that the metabolic changes in the digestive system of mantis shrimp under Cd stress, with 91 metabolites differentially expressed mainly in amino acids, phospholipids, and fatty acid esters. The functional pathways of differential metabolites showed significant effects on biological processes such as amino acid metabolism, transmembrane transport, energy metabolism, and signal transduction. The Cd-induced oxidative stress and energy metabolism disorders were characterized by the differential expression of amino acids and ADP in mantis shrimp, while the interference of transmembrane transport and signal transduction was due to the differential expression of phospholipids.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Yu-Tong Ji, Xiao-Juan Zhou, Qian Yang, Yuan-Biao Lu, Jun Wang, Jie-Xin Zou
Summary: A new species classification scheme of the freshwater crab genus Aparapotamon has been discovered, and mitochondrial genome characteristics and protein-coding genes associated with altitudinal adaptation have been identified. Geological movements of the Qinghai-Tibet Plateau and Hengduan Mountains significantly influenced speciation and differentiation of the genus.
Article
Marine & Freshwater Biology
Jiawen Cui, Yuhao Liu, Zhiyu Hao, Yuhang Liu, Minna Qiu, Lu Kang, Xiaohua Teng, You Tang
Summary: In this experiment, a poisoning model of common carp with cadmium (Cd) exposure was established to assess the nephrotoxicity of Cd. Results showed that Cd exposure induced kidney injury and had an impact on mitochondrial damage and apoptosis. This study provides insights into the underlying mechanisms and theoretical basis for evaluating Cd toxicity to aquatic organisms.
AQUATIC TOXICOLOGY
(2023)
Article
Environmental Sciences
Hui Zhang, Meng Xiu, Hailong Li, Maichao Li, Xiaomei Xue, Yuwei He, Wenyan Sun, Xuan Yuan, Zhen Liu, Xinde Li, Tony R. Merriman, Changgui Li
Summary: The study found that chronic low-dose cadmium exposure can disrupt purine metabolism and energy metabolism, and alter gut homeostasis. Uox-KO mice are more susceptible to cadmium. These findings extend the understanding of potential toxicological interactions between the liver and gut microbiota, and shed light on the progression of metabolic diseases caused by cadmium exposure.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)