Article
Chemistry, Analytical
Yuhong Duan, Lu Xu, Wenyu Song, Hui Gao, Lina Sun, Fangfang Chen, Fen Ma
Summary: In this study, an electrogenerated chemiluminescence (ECL) biosensor based on heterodimerization interaction between CD44 and PEX-14 was developed for highly sensitive quantification of CD44. The biosensor exhibited excellent sensitivity and a wide linear range, making it suitable for detecting CD44 expression in living cells.
MICROCHEMICAL JOURNAL
(2022)
Article
Biotechnology & Applied Microbiology
Yan Li, Juan Wang, Dejian Qian, Liang Chen, Xiumei Mo, Lei Wang, Yan Wang, Wenguo Cui
Summary: Three-dimensional electrospun short fibrous sponges were successfully prepared to mimic the 3D ECM and promote tissue regeneration. The sponges exhibited high porosity, water absorption, and compression resilience, providing an oxygen-rich environment for cell growth and promoting vascularization. In vitro and in vivo studies showed that the sponges were effective in promoting cell proliferation and vascular formation, with better performance in diabetic wound repair compared to 2D micro/nanofiber membranes.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Polymer Science
Kristina Peranidze, Tatiana V. Safronova, Nataliya R. Kildeeva
Summary: Currently, the field of tissue engineering is focused on developing polymer-based materials with suitable microenvironments for cell attachment, differentiation, and proliferation. The biomimicking approach aims to create biocompatible and bioactive materials that mimic the structural features of the natural extracellular matrix. This study discusses promising fibrous materials for bone tissue regeneration obtained through electrospinning techniques, including natural and synthetic polymers combined with bioactive inorganic materials.
Review
Medicine, Research & Experimental
Liang Shan, Fengling Wang, Dandan Zhai, Xiangyun Meng, Jianjun Liu, Xiongwen Lv
Summary: Liver fibrosis is a consequence of chronic liver injuries, characterized by the imbalance of extracellular matrix production and degradation. Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) are crucial in collagen synthesis and lysis. This article reviews experimental studies on liver fibrosis based on MMPs/TIMPs and highlights the potential of targeting this interaction as a therapeutic strategy for hepatic fibrosis. The article also summarizes drugs, including natural products, that have been found to reduce liver fibrosis by altering the ratio of MMPs/TIMPs.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Chemistry, Multidisciplinary
Qimanguli Saiding, Jing Jin, Menglu Qin, Zhengwei Cai, Min Lu, Fei Wang, Wenguo Cui, Xinliang Chen
Summary: The study introduced a new approach utilizing heat-shrinkable electrospun fibrous tape (HS-EFT) to induce contraction of relaxed soft tissues, promoting fibroblast proliferation and extracellular matrix deposition for non-invasive tissue repair. The use of HS-EFT in rabbits showed promising results in reducing wound area and tissue repair, suggesting its potential in structurally and functionally reconstructing relaxed soft tissues.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Plant Sciences
Jose F. Sanchez-Sevilla, Miguel A. Botella, Victoriano Valpuesta, Victoria Sanchez-Vera
Summary: Autophagy is a catabolic and recycling pathway essential for maintaining cellular homeostasis in plants. In cultivated strawberry fruits, autophagy occurs in two waves, characterized by differential expression of ATG genes and ATG8 lipidation. This process plays a significant role in fruit ripening, affecting growth and development.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Engineering, Multidisciplinary
Kun Li, Yingnan Zhang, Junwei Xu, Jingxi Wang, Xuenan Gu, Ping Li, Yubo Fan
Summary: The magnetic PCL/Fe3O4/ICA 3D scaffold, manufactured through electrospinning, exhibited enhanced cell proliferation and infiltration, especially when combined with the static magnetic field, providing a new idea for the design and application of magnetic scaffolds in bone tissue engineering.
COMPOSITES PART B-ENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Xiaoxi Chen, Liu Yang, Run Huang, Sheng Li, Qiangqiang Jia
Summary: The study showed the essential roles of matrix metalloproteinases (MMPs) in the nymph-adult transition of hemimetabolous insects, particularly highlighting the dominant role of PaMmp2 in the imaginal molting process of American cockroach. Inhibition of Mmp activity led to eclosion failure and reduced chitin content, affecting cell interactions and wing expansion.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Article
Cell Biology
Younghoon Jang, Young-Kwon Park, Ji-Eun Lee, Danyang Wan, Nhien Tran, Oksana Gavrilova, Kai Ge
Summary: Our study reveals that MED1 is not generally required for transcription during adipogenesis, but plays a crucial role in postnatal adipose expansion and induction of lipid synthesis genes after diet switch.
GENES & DEVELOPMENT
(2021)
Article
Environmental Sciences
Carlos Ramos, Rebeca Canedo-Mondragon, Carina Becerril, Georgina Gonzalez-Avila, Ana Laura Esquivel, Ana Lilia Torres-Machorro, Martha Montano
Summary: Exposure to short-term wood smoke was found to significantly increase total cells, macrophages, neutrophils, and collagen in the respiratory system of guinea pigs, leading to overexpression of pro-inflammatory cytokines, MMPs, and TIMPs in lung tissue.
Article
Clinical Neurology
Giuseppe Cosentino, Vincenzo Di Stefano, Rosalia Lo Presti, Maria Montana, Massimiliano Todisco, Matteo Gastaldi, Andrea Cortese, Enrico Alfonsi, Cristina Tassorelli, Brigida Fierro, Gregorio Caimi, Filippo Brighina
Summary: This study found that the plasmatic levels of MMPs and TIMPs were significantly altered in patients with CIDP, with higher concentrations of MMP-9, downregulation of TIMP-1, and lower concentration of MMP-2. These new biomarkers may have potential usefulness in the clinical setting and further studies are needed to confirm their utility.
NEUROLOGICAL SCIENCES
(2021)
Article
Chemistry, Analytical
Yuhong Duan, Xinrui Qiao, Lu Xu, Lina Sun, Fen Ma
Summary: A novel sandwich structure electrogenerated chemiluminescence biosensor was developed for determination of matrix metalloproteinase and its expressing cancer cells. The biosensor exhibited excellent sensitivity and a wide linear range.
MICROCHEMICAL JOURNAL
(2022)
Article
Oncology
Yuka Ikeda, Kurumi Taniguchi, Haruka Sawamura, Sayuri Yoshikawa, Ai Tsuji, Satoru Matsuda
Summary: In this article, the invasiveness of cancer cells in relation to APRO family proteins is discussed. The role of matrix metalloproteinases (MMPs) and exosomes containing specific microRNAs in regulating cancer invasiveness is highlighted. Understanding these mechanisms can lead to innovative strategies for cancer therapy.
Article
Multidisciplinary Sciences
Luiz F. Barella, Mario Rossi, Sai P. Pydi, Jaroslawna Meister, Shanu Jain, Yinghong Cui, Oksana Gavrilova, Gianluca Fulgenzi, Lino Tessarollo, Jurgen Wess
Summary: This study found that beta-arrestin-1 (barr1) is essential for beta-cell replication and function in insulin-resistant mice, with deficiency leading to impaired glucose homeostasis possibly through reduced Pdx1 expression levels. Enhancing barr1 activity and expression in beta-cells may be a useful strategy to restore proper glucose homeostasis in type 2 diabetes.
NATURE COMMUNICATIONS
(2021)
Article
Medicine, General & Internal
Tetsuji Morishita, Hiroyasu Uzui, Yusuke Sato, Yasuhiko Mitsuke, Hiroshi Tada
Summary: This study investigated the possible relationships between cachexia, metalloproteinases, and haemodynamics in chronic heart failure patients in Japan, the most aged country in the world. The results showed that cachexia was associated with adverse outcomes and increased activity of MMP species and PVR in this population. Cachexia was also found to be an independent predictor of mortality, with specific biomarkers identified as predictors of cardiac cachexia.
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Cardiac & Cardiovascular Systems
Kim A. Dora, Lyudmyla Borysova, Xi Ye, Chloe Powell, Timea Z. Beleznai, Christopher P. Stanley, Vito D. Bruno, Tobias Starborg, Errin Johnson, Anna Pielach, Michael Taggart, Nicola Smart, Raimondo Ascione
Summary: This study investigated the myogenic reactivity of coronary microvascular smooth muscle cells in patients with heart valve disease and identified a correlation between poor myogenic tone and expression of cellular markers.
CARDIOVASCULAR RESEARCH
(2022)
Editorial Material
Dermatology
J. E. Fromme, P. Zigrino
BRITISH JOURNAL OF DERMATOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Francesca Oltrabella, Anthony Jackson-Crawford, Guanhua Yan, Sarah Rixham, Tobias Starborg, Martin Lowe
Summary: This study reveals that the 27 kDa protein Ipip27A is essential for endocytic trafficking of megalin in the proximal renal tubule of zebrafish larvae and functions together with OCRL in supporting proximal tubule endocytosis. Knockout of Ipip27A leads to endocytic phenotype similar to the loss of OCRL, with reduced uptake of fluid-phase and protein cargo, decreased megalin abundance, and altered endosome morphology. These findings identify Ipip27A as a new player in endocytic traffic in the proximal tubule and suggest that defective endocytosis may underlie renal tubulopathy in Lowe syndrome and Dent-2 disease.
HUMAN MOLECULAR GENETICS
(2022)
Article
Dermatology
Maike Kumper, Sabrina Hessenthaler, Jan Zamek, Stephan Niland, Elke Pach, Cornelia Mauch, Paola Zigrino
Summary: MMP14 plays a critical role in skin physiological and pathological processes, and its deletion in endothelial cells leads to reduced melanoma growth and metastasis through decreased vessel permeability. This suggests that targeting EC-derived MMP14 may be therapeutically crucial in inhibiting melanoma growth and metastasis.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2022)
Article
Cell Biology
Costanza Montagna, Rene B. Svensson, Monika L. Bayer, Salvatore Rizza, Emiliano Maiani, Ching-Yan Chloe Yeung, Giuseppe Filomeni, Michael Kjaer
Summary: Autophagy plays a crucial role in tendon homeostasis and quality control through regulating the secretion of type I procollagen. Activation of autophagy alters the ultrastructural morphology of tendons and negatively affects their biomechanical properties. It may contribute to the development of injured tendons.
CELL DEATH & DISEASE
(2022)
Article
Biology
Joseph Morgan, Rebecca Yarwood, Tobias Starborg, Guanhua Yan, Martin Lowe
Summary: In this study, the functional requirement for the F-BAR protein pacsin2 in endocytosis in the renal proximal tubule of zebrafish larvae was identified. Pacsin2 is enriched at the apical pole of renal tubular epithelial cells and is necessary for efficient endocytosis and maintenance of megalin uptake and recycling.
Article
Multidisciplinary Sciences
Ching-Yan Chloe Yeung, Frank Dondelinger, Erwin M. Schoof, Birgitte Georg, Yinhui Lu, Zhiyong Zheng, Jingdong Zhang, Jens Hannibal, Jan Fahrenkrug, Michael Kjaer
Summary: This study demonstrates the circadian regulation of protein cargo in small extracellular vesicles released by tendon fibroblasts. The abundance of individual proteins in these vesicles shows a 24-hour rhythmic pattern, and the release of vesicles enriched in different protein types is temporally separated. The protein sorting mechanism in the exosome biogenesis pathway, regulated by flotillin-1, plays a role in regulating the abundance of matrix metalloproteinase 14 in tendon fibroblast small extracellular vesicles.
Article
Engineering, Biomedical
Helena Raymond-Hayling, Yinhui Lu, Karl E. Kadler, Tom Shearer
Summary: In this work, a custom fibre tracking algorithm (FTA) was developed to characterize the 3D microstructure of tendons. The algorithm was able to track large numbers of collagen fibrils and handle imaging artefacts associated with electron microscopy. Through the use of FTA, the geometrical properties of collagen fibrils in tendon tissue, such as length, width, alignment, area, and location, were reconstructed and characterized. This algorithm has the potential to shed light on the relationship between tissue microstructure and function in various biological research areas and is freely available for download.
ACTA BIOMATERIALIA
(2022)
Article
Cell & Tissue Engineering
Yoshifumi Tsuchiya, Rene B. Svensson, Ching-Yan Chloe Yeung, Peter Schjerling, Michael Kjaer
Summary: Skeletal muscle has adaptability and regenerative potential due to muscle stem cell activity. The interplay between skeletal muscle and adjacent tendon tissue has not been fully investigated. In this study, we found that factors released by mechanically loaded tendon constructs stimulated myotube formation in human-derived myoblasts. These results suggest the importance of mechanical loading in the signaling interplay between skeletal muscle and tendon tissue for musculoskeletal tissue development and regeneration in humans.
TISSUE ENGINEERING PART A
(2023)
Article
Physiology
Ching-Yan Chloe Yeung, Annesofie T. Olesen, Richard Wilson, Shireen R. Lamande, John F. Bateman, Rene B. Svensson, S. Peter Magnusson, Michael Kjaer
Summary: Both aging and physical activity can affect the amount of intramuscular connective tissue in skeletal muscle. This study investigated the impact on specific extracellular matrix (ECM) proteins and found that aging is associated with an increased abundance of these proteins. However, regular physical activity did not alter the ECM protein levels. These findings indicate that age-related changes in the intramuscular ECM solubility can occur, but physical training does not have the same effect.
JOURNAL OF APPLIED PHYSIOLOGY
(2023)
Article
Cell Biology
Anders Karlsen, Ching-Yan Chloe Yeung, Peter Schjerling, Linda Denz, Christian Hoegsbjerg, Jens R. Jakobsen, Michael R. Krogsgaard, Manuel Koch, Stefano Schiaffino, Michael Kjaer, Abigail L. Mackey
Summary: Through single-nucleus RNA sequencing, we identified distinct clusters of myonuclei in the myotendinous junction (MTJ) of humans, characterized by different gene expression profiles and fiber types, which has important implications for understanding the specialization of the MTJ.
JOURNAL OF CELL SCIENCE
(2023)
Article
Neurosciences
Ching-Yan Chloe Yeung, Rene B. Svensson, Kateryna Yurchenko, Nikolaj M. Malmgaard-Clausen, Ida Tryggedsson, Marius Lendal, Anja Jokipii-Utzon, Jens L. Olesen, Yinhui Lu, Karl E. Kadler, Peter Schjerling, Michael Kjaer
Summary: By analyzing samples from healthy individuals, researchers found that the gene expression in human tendon tissue exhibited day-to-night changes, with a reduction in collagen I expression during the night. However, chronic tendinopathy patients showed fewer differential gene expressions. This indicates the presence of a conserved circadian clock in healthy human patellar tendons.
JOURNAL OF PHYSIOLOGY-LONDON
(2023)
Biographical-Item
Biochemistry & Molecular Biology
Ineke Braakman, Stephen High, Karl Kadler, Roberto Sitia, Kostas Tokatlidis, Philip Woodman
Article
Biochemistry & Molecular Biology
Michal Dudek, Honor Morris, Natalie Rogers, Dharshika R. J. Pathiranage, Sujitha Saba Raj, Danny Chan, Karl E. Kadler, Judith Hoyland, Qing-Jun Meng
Summary: Studies on mice have shown that the sleep-wake cycle is crucial in regulating molecular and cellular processes in intervertebral discs (IVDs), and disruption to this cycle may contribute to IVD degeneration and musculoskeletal diseases. The research also found that mice with disrupted sleep-wake cycles exhibited changes in gene expression patterns in the IVD, leading to alterations in cell fate and extracellular matrix homeostasis. These findings may provide insights into the identification of new molecular targets for treating IVD degeneration.
Article
Cell & Tissue Engineering
Adam J. Janvier, Emily G. Pendleton, Luke J. Mortensen, Daniel C. Green, James R. Henstock, Elizabeth G. Canty-Laird
Summary: This study investigates the effects of different strain levels on fiber generation and structural organization through multimodal analysis, and finds that collagen isoforms play a complex regulatory role in the development of tissue-engineered tendons.
JOURNAL OF TISSUE ENGINEERING
(2022)