Article
Agriculture, Dairy & Animal Science
Yu Zhang, Bu-ting Duan, Yong-juan Zhao, Kai-ling Cui, Tong Xu, Xue-song Zhang, Xiao-ling Lv, Lu-Lu Guo, Ming-xue Zheng, Rui Bai
Summary: Eimeria tenella infection induces autophagy in chicken embryo cecal epithelial cells. The expression of autophagy-related proteins is increased, while p62 protein levels are decreased. Chloroquine enhances autophagy, reducing E. tenella infection, while rapamycin induces autophagy and inhibits E. tenella infection. In contrast, 3-Methyladenine treatment increases E. tenella infection rate.
Article
Immunology
Muhammad Mohsin, Ziping Zhang, Guangwen Yin
Summary: The study investigated the effects of probiotics on coccidiosis in poultry, and found that probiotics improved immune response and antioxidant capacity, as well as serum chemistry, protecting poultry from infection.
Article
Veterinary Sciences
Lixin Xu, Quanjia Xiang, Mongqi Li, Xiaoting Sun, Mingmin Lu, Ruofeng Yan, Xiaokai Song, Xiangrui Li
Summary: This study evaluated the effects of Eimeria mitis on Eimeria necatrix or Eimeria tenella infection and found that the presence of E. mitis exacerbated the outcome of co-infection with E. tenella. However, it had little impact on the infection outcome of E. necatrix. These results suggest the importance of considering the relationships among different Eimeria species when using mixed infection models.
VETERINARY SCIENCES
(2022)
Article
Microbiology
Runhui Zhang, Wanpeng Zheng, Arwid Daugschies, Berit Bangoura
Summary: This study found that the parasites Eimeria tenella and Toxoplasma gondii have significant effects on chickens. Chicken macrophages, as phagocytic cells, participate in the innate immune response against these parasites. The study observed that E. tenella modulates host cell responses to T. gondii, reducing its invasion and replication in chicken macrophages.
Article
Immunology
Hailiang Yu, Changhao Mi, Qi Wang, Wenbin Zou, Guojun Dai, Tao Zhang, Genxi Zhang, Kaizhou Xie, Jinyu Wang, Huiqiang Shi
Summary: This study used high-throughput sequencing to detect the expression profiles of circRNAs in chickens during E. tenella infection, revealing differentially expressed circRNAs that play important roles in the immune response. These findings advance our understanding of circRNA regulatory mechanisms in host resistance and susceptibility to E. tenella infection in chickens.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Veterinary Sciences
Muhammad Mohsin, Lina Li, Xiaohang Huang, Muhammad Tahir Aleem, Yusuf Jibril Habib, Akram Ismael Shehata, Muhammad Zeeshan Afzal, Rao Zahid Abbas, Asghar Abbas, Guangwen Yin
Summary: This study evaluated the cumulative effect of probiotics and EtIMP1C on coccidiosis prevention, with the group administered with both showing less intestinal lesion, reduced oocyst shedding, satisfactory feed conversion ratio (FCR) and improved hematological parameters compared to the group with EtIMP1C alone. Although there was no statistically significant difference between the two groups in terms of lesion and oocyst scores, as well as immunological and hematological parameters, probiotics show promise in combating poultry coccidiosis either alone or in combination with vaccines, including IMP1C based vaccine.
PAKISTAN VETERINARY JOURNAL
(2021)
Article
Biotechnology & Applied Microbiology
F. Q. Memon, Y. Yang, F. Lv, A. M. Soliman, Y. Chen, J. Sun, Y. Wang, G. Zhang, Z. Li, B. Xu, J. A. Gadahi, H. Si
Summary: The results of this study suggest that feed supplementation of probiotic and Bidens pilosa (individually or in combination) is effective in inhibiting the occurrence of disease and decreasing the severity of Eimeria infection in chickens.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Matthew Ogwiji, Isa Danladi Jatau, Joseph Audu Natala, Dahiru Sani, Buhari Habibu, Aliyu Danlami Andamin, Stephen Kyari, Khadijat Abidemi Gasaliyu
Summary: The study evaluated the effects of dietary supplements on gut health, performance, and severity of Eimeria tenella infection in broiler chickens. The results showed that the supplements ameliorated the consequences of coccidiosis in chickens.
TROPICAL ANIMAL HEALTH AND PRODUCTION
(2022)
Article
Parasitology
Arnar K. S. Sandholt, Feifei Xu, Robert Soderlund, Anna Lunden, Karin Troell, Staffan G. Svard, Eva Wattrang
Summary: This study utilized dual RNA-Seq analysis to monitor gene expression of both parasite and host during early stages of Eimeria tenella infection in chicken cells. The results revealed distinct patterns of upregulation and downregulation in immune system genes at different time points, suggesting interactions between parasite invasion and immune system regulation or evasion mechanisms.
Article
Biochemistry & Molecular Biology
Xueting Ma, Baohong Liu, Zhenxing Gong, Zigang Qu, Jianping Cai
Summary: Protein phosphorylation was comprehensively analyzed in different stages of E. tenella life cycle, revealing significant changes in 15247 phosphorylation sites and identifying 456, 479, and 198 differentially expressed phosphoproteins. Gene Ontology and KEGG pathway enrichment analyses of DEPPs indicated their involvement in diverse functions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Igori Balta, Adela Marcu, Mark Linton, Carmel Kelly, Lavinia Stef, Ioan Pet, Patrick Ward, Gratiela Gradisteanu Pircalabioru, Carmen Chifiriuc, Ozan Gundogdu, Todd Callaway, Nicolae Corcionivoschi
Summary: The study demonstrated that the antimicrobial mixture (AG) effectively reduced the infectivity of Eimeria parasites by reducing pathogen virulence and improving host immune response, attenuating the symptoms of coccidiosis.
SCIENTIFIC REPORTS
(2021)
Article
Genetics & Heredity
Peipei Cheng, Zhihao Zhang, Fayu Yang, Shuo Cai, Lina Wang, Chunmei Wang, Mi Wang, Yingchun Liu, Chenzhong Fei, Lifang Zhang, Feiqun Xue, Feng Gu
Summary: This study optimized the genome editing conditions for Eimeria tenella and successfully triggered DNA double-strand breaks using FnCas12a, inserting an enhanced yellow fluorescent protein and a selection marker to create an engineered E. tenella parasite expressing EYFP throughout its lifecycle, thereby enhancing the feasibility of gene function dissection and development of anticoccidial drugs and vaccines for Eimeria species.
FRONTIERS IN GENETICS
(2021)
Article
Agriculture, Dairy & Animal Science
Menggang Li, Xiaoling Lv, Mingxue Zheng, Yingyi Wei
Summary: EtROP27, a pathogenic gene of Eimeria tenella, was successfully expressed as a recombinant protein. It showed promising potential as an antigen gene candidate for the development of a coccidiosis vaccine.
Article
Parasitology
Zhan Liu, Xiangfei Geng, Qiping Zhao, Shunhai Zhu, Hongyu Han, Yu Yu, Wenhao Huang, Yawen Yao, Bing Huang, Hui Dong
Summary: In this study, we found that the transcription and translation levels of vimentin increased continuously after Eimeria tenella sporozoite infection in chickens, and the phosphorylation levels of vimentin also changed. Vimentin accumulated around sporozoites in host cells after infection, and inhibiting vimentin expression significantly improved the invasion efficiency of sporozoites.
PARASITES & VECTORS
(2022)
Article
Parasitology
Li-yun Chang, Ke-qian Di, Jing Xu, Yi-fan Chen, Jian-zhong Xi, De-He Wang, Er-ying Hao, Li-jun Xu, Hui Chen, Rong-yan Zhou
Summary: This study demonstrated that natural garlic essential oil has significant anticoccidial effects on Eimeria tenella infection in chickens. It can reduce clinical symptoms, cecal lesions, oocyst excretion, and increase body weight, while improving intestinal functions and enhancing immune function. Garlic essential oil could potentially be used as an efficient anti-parasitic drug for chicken coccidiosis based on its similar efficacy and relatively low cost compared to diclazuril.
VETERINARY PARASITOLOGY
(2021)
Article
Food Science & Technology
Athina Tzora, Aikaterini Nelli, Anastasia S. Kritikou, Danai Katsarou, Ilias Giannenas, Ilias Lagkouvardos, Nikolaos S. Thomaidis, Ioannis Skoufos
Summary: The effects of different feeding systems on the microbiota and metabolome of Kefalograviera cheese samples were examined in this study. The experimental feeding system was found to alter the metagenomic profile and significantly correlate with specific cheese metabolites.
Article
Agriculture, Dairy & Animal Science
M. Amani-Yengejeh, A. Taghizadeh, H. Mohammadzadeh, A. Hosseinkhani, S. Shirmohammadi, S. Abachi, V. Palangi, H. Eseceli, M. Besharati, I. Giannenas
Summary: In recent years, nitrogen pollution from agro-industrial waste has become a global concern. This study focused on the production of lactosylurea from these waste products and evaluated its nutritive value for ruminant diets. The results showed that lactosylurea had a significant positive impact on feed digestibility and ruminal parameters compared to other non-protein nitrogen sources. It also reduced environmental contamination from agro-industrial waste.
JOURNAL OF ANIMAL AND FEED SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Styliani Lioliopoulou, Georgios A. Papadopoulos, Ilias Giannenas, Konstantina Vasilopoulou, Clare Squires, Paschalis Fortomaris, Fani Th. Mantzouridou
Summary: This study investigated the effects of pomegranate peel byproduct (PPB) supplementation with and without xylanase enzyme on laying hens' diet. The results showed that adding 2.5% PPB and xylanase enhanced yolk coloration and total phenolic content, while adding 5% PPB reduced Malondialdehyde levels in egg yolks.
Review
Agriculture, Dairy & Animal Science
Xuedong Ding, Ilias Giannenas, Ioannis Skoufos, Jing Wang, Weiyun Zhu
Summary: Fat deposition is a crucial factor affecting chicken meat quality, which is closely associated with the lipid metabolism of chickens. Therefore, regulating the lipid metabolism is essential for improving the quality of chicken meat. Plant extracts have been widely used in chicken breeding due to their unique regulatory effects on growth and health. Certain plant extracts have been reported to alter fatty acid composition, decrease abdominal fat percentage, and enhance intramuscular fat content in chickens by improving antioxidant capacity, regulating gene expression, enzymes, and signaling pathways related to lipid metabolism, modulating intestinal microbiota, affecting hormone levels, and regulating DNA methylation. This review paper provides insights into the application and mechanisms of plant extracts in regulating the lipid metabolism of chickens, serving as a reference for future utilization of plant extracts in chicken breeding.
Article
Agriculture, Dairy & Animal Science
Ioannis Nanas, Stella Dokou, Labrini V. Athanasiou, Eleni Dovolou, Thomas M. Chouzouris, Stelios Vasilopoulos, Katerina Grigoriadou, Ilias Giannenas, Georgios S. Amiridis
Summary: This study investigated the effects of partially replacing soybean meal with a mixture of flaxseed and lupins on milk production and fertility parameters in dairy cows during the transition period. The results showed that the dietary treatment had no significant effect on milk yield or composition. However, cows fed with flaxseed and lupin seeds exhibited earlier postpartum estrus and conception compared to the control group. These findings suggest that the substitution of soybean meal with flaxseed and lupin is a profitable feed modification that improves overall fertility in dairy cows.
Article
Agriculture, Dairy & Animal Science
Stella Dokou, Antonios Athanasoulas, Stylianos Vasilopoulos, Zoitsa Basdagianni, Eleni Dovolou, Ioannis Nanas, Katerina Grigoriadou, Georgios S. Amiridis, Ilias Giannenas
Summary: In Mediterranean countries, using locally produced flaxseed and lupins as a partial substitute for soymeal in dairy cow feed can positively affect milk yield and cheese production. This alternative feed does not impact the general health and milk yield of the cows, but it does result in milk and cheese with improved fatty acid profiles that are beneficial to human health. Overall, the substitution of soymeal with flaxseed and lupins is profitable and provides special characteristics to milk and cheese.
Article
Agriculture, Dairy & Animal Science
Tilemachos Mantzios, Vasilios Tsiouris, Georgios A. Papadopoulos, Vangelis Economou, Evanthia Petridou, Georgia D. Brellou, Ilias Giannenas, Costas G. Biliaderis, Konstantinos Kiskinis, Paschalis Fortomaris
Summary: This study investigated the effect of three commercial water acidifiers on the performance, gut health, and C. jejuni colonization in experimentally challenged broiler chicks. The results showed that the tested products did not affect the transmission of C. jejuni from the seeder to sentinel birds. However, all the products ameliorated the adverse effects induced by C. jejuni infection in the physicochemical characteristics of the intestinal content. The continuous use of certain products from the first days of life evoked undesirable effects on broilers' performance and induced severe gross lesions in the upper gastrointestinal tract, leading to the need to modify the dosage scheme in future investigations.
Article
Microbiology
Tilemachos Mantzios, Vasilios Tsiouris, Konstantinos Kiskinis, Vangelis Economou, Evanthia Petridou, Anestis Tsitsos, Apostolos Patsias, Ioanna Apostolou, Georgios A. Papadopoulos, Ilias Giannenas, Paschalis Fortomaris
Summary: This study aimed to determine the antibacterial activity of nine commercial water disinfectants, acidifiers, and glyceride blends against zoonotic pathogens. The results showed promising antibacterial activity for most of the tested products, making them good choices for pathogen control and reducing antimicrobial resistance in poultry farms. Further in vivo studies are recommended to provide more information and determine the optimal dosage scheme and possible synergies of these products.
Article
Food Science & Technology
Irene Bosmali, Ilias Giannenas, Styliani Christophoridou, Christos G. G. Ganos, Aggelos Papadopoulos, Fokion Papathanasiou, Alexandros Kolonas, Olga Gortzi
Summary: This study assessed the impact of genotype, location, and cultivation type on the nutrient and anti-nutrient components of large-seeded bean populations. Significant differences were found in protein, fiber, starch, fat, mineral content, and trypsin inhibitor levels. The location and year of cultivation also influenced the nutritional and antinutritional content of the beans.
Article
Veterinary Sciences
Georgios Magklaras, Ioannis Skoufos, Eleftherios Bonos, Anastasios Tsinas, Christos Zacharis, Ioannis Giavasis, Kostas Petrotos, Konstantina Fotou, Konstantina Nikolaou, Konstantina Vasilopoulou, Ilias Giannenas, Athina Tzora
Summary: In this study, a novel silage made from Greek olive, winery, and feta cheese waste by-products was evaluated as a feed ingredient for weaned pigs. The results showed no negative effects on the pigs' performance, but positive effects on the intestinal microbial populations and meat quality. The development of new sustainable feed sources is crucial for modern animal production systems.
VETERINARY SCIENCES
(2023)
Article
Veterinary Sciences
Christos Zacharis, Eleftherios Bonos, Ilias Giannenas, Ioannis Skoufos, Athina Tzora, Chrysoula (Chrysa) Voidarou, Anastasios Tsinas, Konstantina Fotou, Georgios Papadopoulos, Chrysanthi Mitsagga, Christos Athanassiou, Efthimia Antonopoulou, Katerina Grigoriadou
Summary: The procurement of adequate feed resources is a major challenge for the animal industry worldwide, as the demand for protein-rich feeds exceeds production capacity. This study investigated the use of Tenebrio molitor larvae, fed on either a standard substrate or enriched with plant material, as feed ingredients for growing pigs. The results showed that the larvae meals did not affect pig growth or feed consumption rates, but significantly impacted the fecal microflora balance, blood cholesterol levels, microbial populations in the meat, and its fatty acid profile.
VETERINARY SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Konstantina Vasilopoulou, Georgios A. Papadopoulos, Styliani Lioliopoulou, Ioanna Pyrka, Nikolaos Nenadis, Soumela Savvidou, George Symeon, Vassilios Dotas, Ioannis Panitsidis, Georgios Arsenos, Ilias Giannenas
Summary: This study investigated the effects of olive leaf extract as a feed additive on meat quality in broiler chickens. The inclusion of 1% olive leaf extract showed potential in reducing oxidation in broiler meat and enhancing its quality.
Article
Microbiology
Ioannis Skoufos, Aikaterini Nelli, Brigkita Venardou, Ilias Lagkouvardos, Ilias Giannenas, Georgios Magklaras, Christos Zacharis, Lizhi Jin, Jin Wang, Evangelia Gouva, Stylianos Skoufos, Eleftherios Bonos, Athina Tzora
Summary: The aim of this study was to evaluate the effects of dietary supplementation with an innovative silage on the composition of intestinal microbiota in weaned and finishing pigs. The results showed that the innovative silage could beneficially alter the composition of gastrointestinal microbiota in pigs.
Article
Food Science & Technology
Soumela Savvidou, Nikolas Panteli, Vassilios Dotas, George Symeon, Dimitrios Galamatis, Ioannis Panitsidis, Eirini Voutsinou, Christina Tatidou, Prafulla Kumar, Efthimia Antonopoulou, Georgios Michailidis, Ilias Giannenas
Summary: The present study investigated the effects of dietary supplementation with extracts of pomegranate and onion on meat composition, oxidative stability, cellular signaling pathways, and gene expression. The results showed that the aqueous extract increased moisture content in meat, and protein content in thigh and liver samples. Supplementation with pomegranate and onion extracts improved protein and lipid oxidative stability, enhanced hepatic energy status, and exerted ameliorative effects on stress-related proteins. The encapsulated extract reduced lipid oxidation more effectively, while the aqueous extract had higher total antioxidant capacity and provided better protein protection.
Article
Food Science & Technology
Georgios A. Papadopoulos, Styliani Lioliopoulou, Nikolaos Nenadis, Ioannis Panitsidis, Ioanna Pyrka, Aggeliki G. Kalogeropoulou, George K. Symeon, Alexios-Leandros Skaltsounis, Panagiotis Stathopoulos, Ioanna Stylianaki, Dimitrios Galamatis, Anatoli Petridou, Georgios Arsenos, Ilias Giannenas
Summary: The effects of olive leaf extract on laying hens were evaluated in this study. The results showed that supplementing 1% olive leaf extract in the diet improved egg quality attributes and enhanced antioxidant capacity. Additionally, T2 group had significantly higher eggshell weight and thickness.