Article
Veterinary Sciences
S. Remesar, A. Mendez, A. Benito, A. Prieto, D. Garcia-Dios, C. M. Lopez, R. Panadero, P. Diez-Banos, P. Morrondo, P. Diaz
Summary: In Europe, most cases of canine babesiosis are caused by Babesia canis, Babesia vogeli, and Babesia vulpes. A rapid and efficient multiplex PCR was developed to simultaneously detect and distinguish between large and small forms of the parasite. The multiplex PCR successfully discriminated between the two Babesia groups and can be used as a reliable tool for detecting infections in dogs. It allows for rapid administration of an appropriate treatment, improving the survival rate of infected animals and aiding in understanding the prevalence and distribution of B. vulpes.
RESEARCH IN VETERINARY SCIENCE
(2023)
Article
Parasitology
Katarzyna Tolkacz, Milena Kretschmer, Sabina Nowak, Robert W. Myslajek, Mustafa Alsarraf, Dagmara Wezyk, Anna Bajer
Summary: This study reports the first cases of H. canis infection in dogs and wolves in Poland. The infection manifested as anemia and apathy in older dogs, while young dogs remained asymptomatic. The transmission routes of the virus still remain unclear and further research is needed.
PARASITES & VECTORS
(2023)
Article
Microbiology
Jana Radzijevskaja, Dalyte Mardosaite-Busaitiene, Asta Aleksandravieiene, Birute Karveliene, Migle Razgunaite, Inga Stadaliene, Algimantas Paulauskas
Summary: Canine babesiosis is a rapidly expanding tick-borne disease in central and northeast Europe, and different genotypes of B. canis have been identified in Lithuania.
Article
Parasitology
Agnija Kivrane, Agne Namina, Maija Seleznova, Sarmite Akopjana, Valentina Capligina, Renate Ranka
Summary: A novel duplex RT-PCR molecular method was developed for the detection of B. canis and A. phagocytophilum in canine clinical samples. Sequence variability of Bc28.1 and mdh genes indicated the genetic variability of B. canis and A. phagocytophilum isolates occurring in Latvian domestic dogs.
PARASITES & VECTORS
(2021)
Article
Infectious Diseases
M. Padmaja, Harkirat Singh, Harsh Panwar, Jyoti, Niraj Kumar Singh, Nirbhay Kumar Singh
Summary: Two multiplex SYBR Green based real-time PCR assays were standardized and evaluated to detect DNA from four canine haemoparasites. The assays showed high sensitivity and specificity compared to microscopic evaluation. The study also revealed the prevalence of different haemoparasites and identified risk factors associated with their infections.
TICKS AND TICK-BORNE DISEASES
(2022)
Article
Infectious Diseases
Christina S. Helm, Christiane Weingart, Sabrina Ramuenke, Ingo Schaefer, Elisabeth Mueller, Georg von Samson-Himmelstjerna, Barbara Kohn, Juergen Kruecken
Summary: Since 2015, there has been an increase in autochthonous canine babesiosis cases caused by Babesia canis in the Berlin/Brandenburg area in Germany. Genetic analysis revealed high parasite variability and potential multiple origins for the infections.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Biochemistry & Molecular Biology
Ivana Rubic, Richard Burchmore, Stefan Weidt, Clement Regnault, Josipa Kules, Renata Baric Rafaj, Tomislav Masek, Anita Horvatic, Martina Crnogaj, Peter David Eckersall, Predrag Novak, Vladimir Mrljak
Summary: This study aimed to explore and characterize the serum metabolome of dogs naturally infected with Babesia canis. By using different analytical platforms, 295 metabolites were detected and various metabolic pathways were found to be involved in canine babesiosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Entomology
Anna Grochowska, Justyna Dunaj, Slawomir Pancewicz, Piotr Czupryna, Piotr Majewski, Mulugeta Wondim, Elzbieta Tryniszewska, Anna Moniuszko-Malinowska
Summary: The study in Poland found that Dermacentor reticulatus ticks in urban areas are infected with at least three pathogens, posing a significant public health risk for tick-borne diseases.
EXPERIMENTAL AND APPLIED ACAROLOGY
(2021)
Article
Microbiology
Olga Pawelczyk, Damian Kotela, Marek Asman, Joanna Witecka, Peter Wilhelmsson, Paulina Bubel, Krzysztof Solarz
Summary: Tick-borne microorganisms, including Babesia canis, are important agents causing infectious diseases in humans and animals. This study reports two cases of symptomatic canine babesiosis caused by Babesia canis in domestic dogs from Poland, as well as the presence of Dermacentor reticulatus ticks on one of the infected dogs. Molecular analysis confirmed the presence of Babesia canis in the dogs' blood, with the obtained sequence being identical to a known isolate.
Article
Parasitology
Rafael Chiummo, Eva Zschiesche, Balazs Capari, Robert Farkas, Mathieu Chiquet, Dhimiter Rapti, Rezart Postoli, Alain Audry, Michael Leschnik
Summary: This study demonstrated that the use of fluralaner chewable tablets effectively prevents the transmission of Babesia canis in dogs under natural conditions, with a 100% efficacy rate.
PARASITES & VECTORS
(2023)
Article
Parasitology
Tera Pijnacker, Richard Bartels, Martin van Leeuwen, Erik Teske
Summary: This study aimed to identify ADVIA hematology analyzer parameters that suggest B. cants parasitemia in dogs and develop a predictive model. Both conventional statistical methods and machine learning methods were used, and important parameters including platelet count, mean platelet volume, and percentage of large unstained cells were identified. The study successfully formulated models with high sensitivity and specificity, suggesting that the algorithms can be easily integrated into laboratories using ADVIA analyzers to screen for Babesia parasitemia in dogs.
PARASITES & VECTORS
(2022)
Article
Parasitology
Dorota Dwuznik-Szarek, Ewa J. Mierzejewska, Anna Rodo, Katarzyna Gozdzik, Jolanta Behnke-Borowczyk, Dorota Kiewra, Natalia Kartawik, Anna Bajer
Summary: The study demonstrates the continuous expansion of two populations of D. reticulatus ticks in Poland, mainly along river basins. There is a marked colonization of the gap zone and a decreasing distance between Western and Eastern tick populations. The incidence of canine babesiosis is lower in Western Poland and the gap area, while higher in Central and Eastern Poland, suggesting strong associations between tick distribution and occurrence of babesiosis.
PARASITES & VECTORS
(2021)
Article
Microbiology
Anna Bajer, Maciej Kowalec, Viktoriya A. Levytska, Ewa Julia Mierzejewska, Mustafa Alsarraf, Vasyl Poliukhovych, Anna Rodo, Dagmara Wezyk, Dorota Dwuznik-Szarek
Summary: This study found a high prevalence of Babesia spp. and Borrelia burgdorferi s.l. infections in pet dogs from Central and North-Eastern Europe, but not in sled dogs and Baltic countries.
Article
Biology
Mariana Ionita, Laurentiu Leica, Marion Wassermann, Emanuel Mitrea, Isabela Madalina Nicorescu, Ioan Liviu Mitrea
Summary: This study aimed to investigate the genetic diversity of Babesia spp. strains in a tick-endemic area in southeastern Romania. It identified the presence of both Babesia canis and Babesia vogeli strains, with different genotypes of B. canis. It also revealed the first evidence of genetic variability in canine babesiosis in Romania, providing a basis for further studies on the disease.
Article
Multidisciplinary Sciences
Abdelfattah Selim, Ameer Megahed, Mourad Ben Said, Abdullah D. Alanazi, Mohamed Z. Sayed-Ahmed
Summary: This study investigated the prevalence of B. vogeli in dogs from Egypt using PCR assay and evaluated the associated risk factors. The prevalence rate of B. vogeli was 5.1%, with tick infestation and the type of dog shelter floor identified as potential risk factors. Phylogenetic analysis revealed the Egyptian isolate to be grouped with isolates from Japan, Venezuela, and Paraguay. The findings contribute to the understanding of canine babesiosis epidemiology and disease control in companion dogs.
SCIENTIFIC REPORTS
(2022)
Article
Agriculture, Dairy & Animal Science
Consuelo Borie-Polanco, Nicolas Galarce-Galvez, Karina Yevenes-Coa, Jose-Manuel Yanez-Lopez, James Robeson-Camus, Alfonso Carbonero-Martinez
Summary: The experiment showed that the addition of a bacteriophage cocktail in a mayonnaise-like matrix significantly reduced the SE counts, demonstrating the effectiveness of bacteriophages as biocontrol agents for SE. Further studies are needed to confirm the reduction in undiluted homemade mayonnaise.
REVISTA COLOMBIANA DE CIENCIAS PECUARIAS
(2021)
Article
Agriculture, Dairy & Animal Science
Luis R. Saa, Lucia T. Guzman, Natacha C. Fierro, Luz M. Castro, Fabian Reyes-Bueno, Alfonso Carbonero
Summary: Bovine leukemia virus (BLN) is widespread in cattle herds in Ecuador, with individual and herd seroprevalence rates of 17.3% and 37.8% respectively. Risk factors associated with BLN seropositivity include artificial insemination, concrete floors, presence of wild ruminants, and sampling season. A control program should focus on these identified risk factors.
REVISTA COLOMBIANA DE CIENCIAS PECUARIAS
(2021)
Article
Veterinary Sciences
Lukasz Adaszek, Anna Wilczynska, Jerzy Zietek, Marcin Kalinowski, Oliwier Teodorowski, Dagmara Winiarczyk, Maciej Skrzypczak, Stanislaw Winiarczyk
Summary: This study presented the first clinical case of granulocytic anaplasmosis in a captive ring-tailed lemur in Poland. The 4-year-old female lemur showed symptoms including anorexia, epistaxis, and tick infestation. Treatment with doxycycline for 3 weeks resulted in the lemur's recovery within 24 hours, highlighting the importance of considering A. phagocytophilum infection in differential diagnosis, especially in cases of thrombocytopenia associated with Ixodes ricinus parasitism in this animal species.
BMC VETERINARY RESEARCH
(2021)
Article
Toxicology
Anna Rymuszka, Anna Sieroslawska, Lukasz Adaszek
Summary: Nodularin, a cyclic peptide released by toxic cyanobacteria, causes cytotoxic and immunotoxic effects depending on cell type and toxin concentration. These effects include cytotoxicity, inhibition of chemotaxis, decreased proliferation of lymphocytes, increased production of reactive oxygen and nitrogen species, and altered gene expression of cytokines. While the observed changes in cytokine mRNA levels were evident, they were not clearly dose-dependent.
JOURNAL OF APPLIED TOXICOLOGY
(2021)
Article
Infectious Diseases
Jose Manuel Diaz-Cao, Lukasz Adaszek, Beata Dziegiel, Jorge Paniagua, Javier Caballero-Gomez, Stanislaw Winiarczyk, Dagmara Winiarczyk, David Cano-Terriza, Ignacio Garcia-Bocanegra
Summary: A survey study conducted in southern Spain's Mediterranean ecosystems aimed to assess the prevalence of selected tick-borne pathogens (TBP) in wild ungulates. The study found TBP DNA in various wild ruminants, with different species showing different prevalences. The role of wild ruminants in maintaining TBP in Mediterranean ecosystems was suggested, while the contribution of wild boar seemed limited in the region.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Veterinary Sciences
Oliwier Teodorowski, Marcin Kalinowski, Dagmara Winiarczyk, Radoslaw Janecki, Stanislaw Winiarczyk, Lukasz Adaszek
Summary: The study conducted molecular surveillance of piroplasmosis, granulocytic anaplasmosis, and Lyme borreliosis in horses from Poland. The results showed a relatively low prevalence of these diseases, but the need for consideration in differential diagnosis of related symptoms.
VETERINARY MEDICINE AND SCIENCE
(2021)
Article
Reproductive Biology
Michal Wieszczeczy, Leszek Krakowski, Grzegorz Opielak, Izabela Krakowska, Jacek Furmaga, Piotr Brodzki, Mariola Bochniarz, Roman Dabrowski, Tomasz Piech, Barbara Zdzisinska, Lukasz Adaszek
Summary: The study confirmed the significant role of miRNA-129 and VEGF in diagnosing BPH in dogs, helping differentiate dogs with BPH and contribute to the diagnosis of prostate disorders.
Article
Medicine, General & Internal
Mateusz Winiarczyk, Dagmara Winiarczyk, Katarzyna Michalak, Kai Kaarniranta, Lukasz Adaszek, Stanislaw Winiarczyk, Jerzy Mackiewicz
Summary: Macular edema and its complications from choroidal neovascularization are leading causes of blindness in elderly with AMD. A study found 11 significantly differentially expressed proteins in tears of neovascular AMD patients, related to impaired protein clearance, inflammation, and neovascularization signaling pathways. Tear film protein analysis is a novel approach for AMD biomarker screening.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Veterinary Sciences
A. Carbonero, A. Maldonado-Iniesta, Y. Trujillo, J. Perea, M. Riofrio, I Garcia-Bocanegra, C. Borge
Summary: This study aimed to determine the prevalence of three genes (htrA, htrB, and ppk1) related to environmental persistence in C. jejuni and C. coli isolates obtained from abattoir samples at different stages. The overall prevalence of these genes was 40.3%, 93.3%, and 68.9%, respectively. Significant differences were found between the prevalence in C. jejuni and C. coli, with higher prevalence of htrA and htrB in C. coli, and higher prevalence of ppk1 in C. jejuni. Furthermore, a significant increase in the frequency of htrA and htrB genes in the final meat products compared to initial broiler samples was observed in C. jejuni. These results suggest the involvement of htrA and htrB genes in the environmental persistence of Campylobacter jejuni.
VETERINARY RESEARCH COMMUNICATIONS
(2022)
Article
Veterinary Sciences
Oleh M. Fedets, Kostyantyn V. Dmytruk, Lukasz Adaszek, Iryna M. Kurlyak, Olena V. Dmytruk, Urszula Lisiecka, Stanislaw Winiarczyk
Summary: This study found an association between single nucleotide polymorphisms (SNPs) in the glutathione S-transferase P1 (GSTP1) gene and mammary tumors in dogs. Dogs with mammary tumors showed significant differences in GSTP1 SNPs compared to healthy dogs. This suggests that GSTP1 SNPs may be associated with the occurrence of mammary tumors in dogs.
VETERINARY AND COMPARATIVE ONCOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Anna Wilczynska, Renata Komsta, Mateusz Szadkowski, Jerzy Zietek, Lukasz Adaszek
Summary: This study analyzed the prevalence of E. cuniculi infection in guinea pigs with different clinical disorders. The results showed that encephalitozoonosis is a significant problem in rodents kept as domestic animals and may be a source of infection for humans.
Review
Medicine, Research & Experimental
Anna Wilczynska, Jerzy Zietek, Oliwier Teodorowski, Lukasz Adaszek
Summary: Encephalitzoon spp. are microsporidia that can infect both humans and animals, particularly immunocompromised individuals. Recent discoveries of new microsporidial species have raised questions about their natural origin and their potential as zoonotic pathogens.
POSTEPY HIGIENY I MEDYCYNY DOSWIADCZALNEJ
(2022)
Article
Veterinary Sciences
O. Teodorowski, S. Winiarczyk, P. Debiak, M. Skrzypczak, L. Mazurek, L. Adaszek
Summary: This study aimed to analyze cases of granulocytic anaplasmosis in 53 hunting dogs in Poland and found that lethargy, inappetence, and fever were the most common clinical signs in A. phagocytophilum-positive dogs. The most common laboratory abnormality was thrombocytopenia, and the recovery rate was 96%, with a mortality rate of 4%. This indicates the importance of considering A. phagocytophilum infection in differential diagnosis in dogs, especially in hunting dogs with certain symptoms and tick invasions.
POLISH JOURNAL OF VETERINARY SCIENCES
(2021)
Article
Veterinary Sciences
Oliwier Teodorowski, Stanislaw Winiarczyk, Duygu Tarhan, Banu Dokuzeylul, Alev Meltem Ercan, Mehmet Erman Or, Marta Staniec, Lukasz Adaszek
Summary: This study investigated the link between uncomplicated Babesia canis infection in dogs and blood concentrations of zinc and copper, as well as erythrocytic antioxidant defence. Results showed that infected dogs had higher levels of antioxidant enzymes and lower levels of blood micronutrients compared to uninfected dogs. Additionally, oxidative stress was proposed as a mechanism leading to anemia in dogs with babesiosis.
JOURNAL OF VETERINARY RESEARCH
(2021)
Article
Veterinary Sciences
L. Guz, J. Wawrzykowski, L. Adaszek
Summary: This study screened the in vitro activity of essential oil from Achillea millefolium against Babesia canis, revealing its anti-babesial properties. The essential oil was found to contain a variety of biologically active compounds, with chamazulene, beta-caryophyllene, and (E)-germacrene D as the most abundant components.
POLISH JOURNAL OF VETERINARY SCIENCES
(2021)
