Article
Biochemical Research Methods
Soren M. Karst, Ryan M. Ziels, Rasmus H. Kirkegaard, Emil A. Sorensen, Daniel McDonald, Qiyun Zhu, Rob Knight, Mads Albertsen
Summary: High-throughput amplicon sequencing of large genomic regions remains challenging for short-read technologies. Here, a new approach combining unique molecular identifiers (UMIs) with Oxford Nanopore Technologies (ONT) or Pacific Biosciences circular consensus sequencing was reported, achieving high-accuracy single-molecule consensus sequences. The method was successfully applied to sequence ribosomal RNA operon amplicons and genomic sequences of microbial communities, showing a very low chimera rate.
Review
Agriculture, Dairy & Animal Science
Stephanie Buchheister, Andre Bleich
Summary: Ensuring animal health and standardization in biomedical research requires monitoring infectious agents and considering the impact of microbiome on research results to enhance validity and reliability. The evolution of monitoring methods and the development of strategies to cope with microbiome variation are crucial for increasing reproducibility and generalizability in animal experiments.
Article
Genetics & Heredity
Sarah V. Emser, Helmut Schaschl, Eva Millesi, Ralf Steinborn
Summary: By utilizing single long-range PCR with back-to-back primers targeting a consensus sequence motif in Vertebrata, this study successfully extended the application to sequencing mitogenomes of hibernating rodents, providing valuable insights into mitochondrial-derived peptides (MDPs) and potential polymorphisms associated with cold adaptation. The versatility and sensitivity of this approach make it promising for future studies on mitochondrial genomics in a wide range of vertebrate species.
FRONTIERS IN GENETICS
(2021)
Article
Plant Sciences
Manuel Anguita-Maeso, Carmen Haro, Juan A. Navas-Cortes, Blanca B. Landa
Summary: This study evaluated the effects of extraction methods, plant age, and PCR primers on the characterization of xylem bacterial communities. Different extraction methods and PCR primers resulted in significant biases in describing the bacterial community. The relative importance of dominant bacterial genera varied depending on the primers and extraction methods used.
Article
Agriculture, Dairy & Animal Science
Tahani Salama Behour, Eman Mohamed Abd El Fattah
Summary: In this study, the genotype of Trypanosoma brucei evansi (T. b. evansi) in Egyptian camels was analyzed and a new variant of non-RoTat 1.2 T. b. evansi was discovered. The prevalence of RoTat 1.2 T. b. evansi was found to be higher compared to the non-RoTat 1.2 type, with the latter only detected in Aswan samples.
TROPICAL ANIMAL HEALTH AND PRODUCTION
(2023)
Article
Multidisciplinary Sciences
Abdelfattah Selim, Hayat Ali Alafari, Kotb Attia, Muneera D. F. AlKahtani, Fatima M. Albohairy, Ibrahim Elsohaby
Summary: Surra is a parasitic disease caused by Trypanosoma evansi (T. evansi) and transmitted by biting flies. A study in three Egyptian governorates found a high prevalence of T. evansi infection in camels, with older camels, spring season, females, and poor conditioned camels being more susceptible. The card agglutination test for T. evansi showed higher sensitivity than the thin blood smear method and both tests had perfect specificity. The findings suggest that the card agglutination test is a useful tool for the routine control of trypanosomiasis in camels.
SCIENTIFIC REPORTS
(2022)
Article
Infectious Diseases
Daniel Bravo-Barriga, Martina Ferraguti, Sergio Magallanes, Pilar Aguilera-Sepulveda, Francisco Llorente, Elisa Perez-Ramirez, Ana Vazquez, Fatima Guerrero-Carvajal, Maria Paz Sanchez-Seco, Miguel Angel Jimenez-Clavero, Carlos Mora-Rubio, Alfonso Marzal, Eva Frontera, Florentino de Lope
Summary: Usutu virus (USUV) has caused an increasing number of animal and human cases in Europe. The circulation of USUV in Extremadura, Spain was investigated through mosquito and bird sampling. USUV was detected in two pools of Culex pipiens mosquitoes, and USUV-specific antibodies were found in 17 wild birds from eight species. This indicates active circulation of USUV and a potential risk of exposure for the human population.
TRANSBOUNDARY AND EMERGING DISEASES
(2023)
Article
Agriculture, Multidisciplinary
Yohan Charbonnier, Daciana Papura, Olivier Touzot, Noriane Rhouy, Gilles Sentenac, Adrien Rusch
Summary: The study found that bats increase their hunting activity significantly when European grapevine moths are present in vineyards. Molecular analysis shows that at least 10 species of bats prey on the three grapevine moth species. This suggests that bats can be natural enemies of grape pests in vineyard landscapes.
AGRICULTURE ECOSYSTEMS & ENVIRONMENT
(2021)
Article
Plant Sciences
Gi Yoon Shin, Amy Smith, Teresa A. Coutinho, Bhabesh Dutta, Brian H. Kvitko
Summary: Species of Pantoea are plant pathogenic bacteria that infect economically important plant species. This study evaluated the specificity of different primers for rapid identification of Pantoea species and found that some primers showed high specificity and accuracy.
Article
Medicine, General & Internal
Agnieszka Sroka-Oleksiak, Wojciech Pabian, Joanna Sobonska, Kamil Drozdz, Tomasz Bogiel, Monika Brzychczy-Wloch
Summary: The aim of this study was to evaluate the sensitivity of group B Streptococcus (GBS) detection using polymerase chain reaction primers and a preincubation step. Vaginal and rectal swabs were collected from 97 pregnant women and subjected to enrichment broth culture-based diagnostics and amplification using specific primers. The preincubation step increased the sensitivity of GBS detection, and additional GBS cases were identified using nucleic acid amplification techniques. The atr gene primers showed the highest number of true positive results compared to other primers.
Article
Immunology
Faleh A. Alfaleh, Mahmoud M. Elhaig
Summary: This study estimated the prevalence of T. evansi infection and its associated risk factors in camels in Egypt. The overall prevalence was found to be 19.6%, with higher infection rates in female camels and camels with clinical signs. Genetic analysis confirmed the presence of two distinct genotypes of T. evansi in Egyptian camels, closely related to isolates from the Philippines and China.
MICROBIAL PATHOGENESIS
(2023)
Article
Plant Sciences
Meagan Van Dyk, Christoffel F. J. Spies, Lizel Mostert, Francois Halleen
Summary: This study reveals that pruning debris serves as an inoculum source of P. globosa in established olive orchards in South Africa. It emphasizes the importance of reducing the inoculum sources of this pathogen as an additional management strategy against olive trunk diseases.
Article
Biochemistry & Molecular Biology
Daniel Vaulot, Stefan Geisen, Frederic Mahe, David Bass
Summary: Metabarcoding of microbial eukaryotes has greatly advanced in the past decade, with the development of a database listing 285 primers and 83 unique primer pairs used for eukaryotic 18S rRNA gene metabarcoding. An R-based web application has been developed to assist researchers in making informed primer choices and including protists in their investigations.
MOLECULAR ECOLOGY RESOURCES
(2022)
Article
Agronomy
Caroline Ariyoshi, Gustavo Hiroshi Sera, Lucas Mateus Rivero Rodrigues, Filipe Gimenez Carvalho, Luciana Harumi Shigueoka, Ana Ester Socatelli Mendonca, Carlos Theodoro Motta Pereira, Suzete Aparecida Lanza Destefano, Luiz Filipe Protasio Pereira
Summary: Bacterial halo blight (BHB) is a bacterial disease in coffee-producing regions. Development of resistant cultivars is a sustainable alternative to chemical control. The study identified a marker linked to resistance and developed a cost-effective method for marker-assisted selection (MAS) in breeding programs.
Article
Food Science & Technology
Hela Gargouri, Nizar Moalla, Hassen Hadj Kacem
Summary: The study recommends using PCR-RFLP and PCR techniques for meat species detection, detecting multiple animal species and identifying 10 animal species in daily meat analysis.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2021)
Article
Veterinary Sciences
Amanda E. Fine, Mathieu Pruvot, Camilla T. O. Benfield, Alexandre Caron, Giovanni Cattoli, Philippe Chardonnet, Maurizio Dioli, Thomas Dulu, Martin Gilbert, Richard Kock, Juan Lubroth, Jeffrey C. Mariner, Stephane Ostrowski, Satya Parida, Sasan Fereidouni, Enkhtuvshin Shiilegdamb, Jonathan M. Sleeman, Claudia Schulz, Jean-Jacques Soula, Yves Van der Stede, Berhe G. Tekola, Chris Walzer, Steffen Zuther, Felix Njeumi
FRONTIERS IN VETERINARY SCIENCE
(2020)
Article
Multidisciplinary Sciences
Nguyen Quynh Huong, Nguyen Thi Thanh Nga, Nguyen Van Long, Bach Duc Luu, Alice Latinne, Mathieu Pruvot, Nguyen Thanh Phuong, Le Tin Vinh Quang, Vo Van Hung, Nguyen Thi Lan, Nguyen Thi Hoa, Phan Quang Minh, Nguyen Thi Diep, Nguyen Tung, Van Dang Ky, Scott Roberton, Hoang Bich Thuy, Martin Gilbert, Leanne Wicker, Jonna A. K. Mazet, Christine Kreuder Johnson, Tracey Goldstein, Alex Tremeau-Bravard, Victoria Ontiveros, Damien O. Joly, Chris Walzer, Amanda E. Fine, Sarah H. Olson
Article
Infectious Diseases
Timothee Vergne, Mathieu Andraud, Sarah Bonnet, Nick De Regge, Marc Desquesnes, Johanna Fite, Florence Etore, Mutien-Marie Garigliany, Ferran Jori, Laetitia Lempereur, Marie-Frederique Le Potier, Elsa Quillery, Claude Saegerman, Laurence Vial, Emilie Bouhsira
Summary: Stable flies may play a minor role in the spread of ASFV at low abundances, but could be responsible for a significant proportion of transmission events at higher abundances, potentially reducing time to pig mortality. The relative contribution of stable flies is strongly influenced by factors like blood volume regurgitated and infectious dose for pigs.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Public, Environmental & Occupational Health
Chanfong Philavong, Mathieu Pruvot, Daniel Reinharz, Mayfong Mayxay, Kongsy Khammavong, Phonesavanh Milavong, Sayapeth Rattanavong, Paul F. Horwood, Philippe Dussart, Bounlom Douangngeun, Watthana Theppangna, Amanda E. Fine, Matthew T. Robinson, Paul N. Newton
ZOONOSES AND PUBLIC HEALTH
(2020)
Article
Multidisciplinary Sciences
Kirsty Officer, Mathieu Pruvot, Paul Horwood, Daniela Denk, Kris Warren, Vibol Hul, Nhim Thy, Nev Broadis, Philippe Dussart, Bethany Jackson
Review
Parasitology
Marc Desquesnes, Marisa Gonzatti, Alireza Sazmand, Sophie Thevenon, Geraldine Bossard, Alain Boulange, Geoffrey Gimonneau, Philippe Truc, Stephane Herder, Sophie Ravel, Denis Sereno, Vincent Jamonneau, Sathaporn Jittapalapong, Philippe Jacquiet, Philippe Solano, David Berthier
Summary: This review discusses the reliable and up-to-date methods for diagnosing trypanosomoses caused by trypanosomes in wild and domestic mammals. The diseases have significant impact on human and animal health, as well as livestock economy. The review covers various diagnostic methods including parasite detection, DNA detection, and antibody detection, while considering factors such as sensitivity, specificity, and cost constraints. It also addresses the challenge of diagnosing mixed infections and the importance of improving diagnostic procedures worldwide for better infection control and One Health.
PARASITES & VECTORS
(2022)
Editorial Material
Parasitology
Alireza Sazmand, Marc Desquesnes, Domenico Otranto
TRENDS IN PARASITOLOGY
(2022)
Article
Microbiology
Phirabhat Saengsawang, Serge Morand, Marc Desquesnes, Sarawut Yangtara, Tawin Inpankaew
Summary: This study detected Bartonella spp. in rodents from urban and suburban areas in Thailand, with approximately 38% of the rodents testing positive for Bartonella DNA. The Pacific rat and Asian house rat were found to harbor Bartonella spp., with four species identified in blood samples. Specific species complexes were found in particular habitats, indicating a need for a monitoring program for zoonotic Bartonella infection in high-risk areas.
Article
Agriculture, Dairy & Animal Science
Eukote Suwan, Piangjai Chalermwong, Rucksak Rucksaken, Metita Sussadee, Sarawan Kaewmongkol, Ruenruetai Udonsom, Sathaporn Jittapalapong, Bandid Mangkit
Summary: This study developed and evaluated a pETite-GRA7-based indirect ELISA method for detecting Toxoplasma gondii infection in cats. The results showed high sensitivity and specificity, with good concordance with existing diagnostic methods.
Review
Biochemistry & Molecular Biology
Denis Sereno, Bruno Oury, Anne Geiger, Andrea Vela, Ahmed Karmaoui, Marc Desquesnes
Summary: Isothermal amplification of nucleic acids has the potential to be applied for the detection of infectious agents in resource-limited areas. This review focuses on the application of this technique for Trypanosoma and Leishmania infections and proposes ways to improve its translation into real-world field applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Parasitology
Marc Desquesnes, Alireza Sazmand, Marisa Gonzatti, Alain Boulange, Geraldine Bossard, Sophie Thevenon, Geoffrey Gimonneau, Philippe Truc, Stephane Herder, Sophie Ravel, Denis Sereno, Etienne Waleckx, Vincent Jamonneau, Philippe Jacquiet, Sathaporn Jittapalapong, David Berthier, Philippe Solano, Laurent Hebert
Summary: Reliable diagnostic tools are necessary to effectively treat and control animal trypanosomoses, which can be pathogenic. This review emphasizes the implementation of previously reviewed diagnostic methods in complex scenarios, and highlights the need for an integrative approach that combines parasite detection, DNA/RNA/antigen detection, antibody detection, and epizootiological information. While antibody detection tests have high sensitivity, DNA-based methods are more specific. Further developments are required to address gaps in current diagnostic methods and to improve prevention and control of animal trypanosomoses.
PARASITES & VECTORS
(2022)
Article
Entomology
Marc Desquesnes, Kornkanok Thaisungnoen, Piangjai Chalermwong, Adele Nevot, Clement Fossaert, Antoine Lucas, Sathaporn Onju, Phoompong Boonsaen, Sathaporn Jittapalapong
Summary: This study evaluated the use of pedometers attached to the tails of feeder cattle to assess tail flick frequency and evaluate the impact of hematophagous flies. The results showed a strong correlation between fly density and both visual and pedometer records of tail flicks. The pedometers were able to reveal a high nuisance of mosquitoes that should be further investigated. In conclusion, the tail pedometers proved to be useful tools in evaluating fly density and the impact of dipterans on cattle behavior.
Article
Biochemistry & Molecular Biology
Warin Rangubpit, Eukote Suwan, Danai Sangthong, Kannika Wongpanit, Roger W. Stich, Prapasiri Pongprayoon, Sathaporn Jittapalapong
Summary: In this study, molecular dynamics simulations were conducted to investigate the binding of GTX and GSH to RmGST. It was found that GSH binds tighter and sits rigidly inside the G-site, while GTX occupies both active sites.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Chanchanok Duangsri, Tiina A. Salminen, Marion Alix, Sarawan Kaewmongkol, Nattaphong Akrimajirachoote, Wanthanee Khetkorn, Sathaporn Jittapalapong, Pirkko Maenpaa, Aran Incharoensakdi, Wuttinun Raksajit
Summary: Polyhydroxybutyrate (PHB) is a biocompatible and biodegradable polymer with potential applications as a replacement for fossil-derived polymers. The key enzyme for PHB production in Arthrospira platensis is PHA synthase (PhaC). This study constructed recombinant E. cloni ((R))10G cells carrying the A. platensis phaC gene (rPhaC(Ap)) and characterized the catalytic properties and structure of the overexpressed rPhaC(Ap). The obtained model of PhaC(Ap) revealed its asymmetric dimeric structure and the involvement of specific residues in substrate binding and dimerization.
Article
Parasitology
Maria Teresa Tejedor-Junco, Adrian Melian Henriquez, Pedro Pelaez Puerto, Maria Dolores Ramos, Margarita Gonzalez-Martin, Manuel Morales Doreste, Geoffrey Gimonneau, Marc Desquesnes, Sergio Martin Martel, Juan Alberto Corbera
Summary: This study investigates the occurrence of Surra, a disease caused by Trypanosoma evansi, in camels in the Canary Islands. Control measures implemented in 1997 led to a decrease in prevalence, but an outbreak in 2014 necessitated the establishment of a lazaret for isolation and further control measures. The only vector for the disease was Stomoxys calcitrans, and no other animals were found to be positive for T. evansi. The study provides evidence to aid in the control of African Animal Trypanosomosis in endemic areas and may contribute to revising control strategies for animal Trypanosomosis in camels.