Review
Infectious Diseases
Ina Gajic, Jovana Kabic, Dusan Kekic, Milos Jovicevic, Marina Milenkovic, Dragana Mitic Culafic, Anika Trudic, Lazar Ranin, Natasa Opavski
Summary: Antimicrobial resistance poses a significant threat to global public health. Accurate and rapid detection of antibiotic resistance and appropriate treatment methods are crucial for controlling its emergence. However, traditional testing methods are time-consuming and costly, highlighting the need for innovative approaches.
Article
Infectious Diseases
Anna Vilaro, Elena Novell, Vicens Enrique-Tarancon, Jordi Balielles, Lourdes Migura-Garcia, Lorenzo Fraile
Summary: Antimicrobial susceptibility testing is important for antimicrobial stewardship, but limited resources often restrict the number of drugs that can be tested for each antimicrobial family. This study examined the minimal inhibitory concentration for various bacterial strains collected in Spain, and found that there were generally no significant associations between MIC patterns for different antimicrobial families. However, some drug-bug combinations showed high correlation, indicating that MIC values from one representative drug could be indicative of the whole antimicrobial family.
Article
Microbiology
Xi Wang, W. Evan Chaney, Hilary O. Pavlidis, James P. McGinnis, J. Allen Byrd, Yuhua Z. Farnell, Timothy J. Johnson, Audrey P. McElroy, Morgan B. Farnell
Summary: Comparing different isolation and antimicrobial susceptibility testing methods on Salmonella isolated from poultry samples, the study found that methodology plays a critical role in detecting antimicrobial resistance phenotypes. Results showed discrepancies in resistance profiles depending on the methods used, highlighting the importance of selecting appropriate methodology for accurate surveillance of antimicrobial resistance in poultry pathogens.
Article
Microbiology
Tomefa E. Asempa, Christian M. Gill, Vindana Chibabhai, David P. Nicolau
Summary: This study investigates the zinc concentrations in commonly used automated susceptibility testing platforms and reveals significant variability. The findings pose challenges to the development of zinc-limited media.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Tamara Alhamami, Wai Yee Low, Yan Ren, Kara Taylor, Manouchehr Khazandi, Tania Veltman, Henrietta Venter, Mandi Carr, Conny Turni, Sam Abraham, Darren J. Trott
Summary: This study investigated the antimicrobial susceptibility and phylogenetic relationships of H. somni isolates from Australian feedlot cattle. The results showed that these isolates did not exhibit resistance to the tested antimicrobials and had limited genetic diversity.
VETERINARY MICROBIOLOGY
(2022)
Article
Veterinary Sciences
William B. Crosby, Brandi B. Karisch, Lari M. Hiott, Lee J. Pinnell, Alexandra Pittman, Jonathan G. Frye, Charlene R. Jackson, John Dustin Loy, William B. Epperson, John Blanton, Sarah F. Capik, Paul S. Morley, Amelia R. Woolums
Summary: This study aimed to evaluate the impact of tulathromycin metaphylaxis on antimicrobial resistance in MH isolated from stocker calves, and found that metaphylaxis increased the risk of isolation of multidrug resistant MH. However, the antimicrobial resistance genes identified in the isolated MH were primarily associated with integrative conjugative elements (ICE).
FRONTIERS IN VETERINARY SCIENCE
(2023)
Review
Immunology
Tucker Maxson, Thiphasone Kongphet-Tran, Thitipong Mongkolrattanothai, Tatiana Travis, Katherine Hendricks, Corinne Parker, Heather P. McLaughlin, Julia Bugrysheva, Frank Ambrosio, Pierre Michel, Blake Cherney, Christine Lascols, David Sue
Summary: Research has shown that most Bacillus anthracis isolates are susceptible to current first-line antimicrobials recommended for postexposure prophylaxis and treatment. Data on potential alternative antimicrobials will be helpful in case of significant resistance to first-line antimicrobials, bioengineered strains, or unavailability/tolerance issues with first-line antimicrobials.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Infectious Diseases
Lukas Lueftinger, Peter Majek, Thomas Rattei, Stephan Beisken
Summary: This study investigates the performance of in-silico antimicrobial susceptibility testing (AST) from clinical metagenomic data. Using simulated metagenomes with known resistance phenotype, the impact of various factors on AST accuracy is explored. The results show that an optimized metagenomics assembly and binning workflow can improve AST accuracy, and machine learning-based methods outperform rule-based approaches on simulated patient samples.
Review
Microbiology
Szilvia Neuhaus, Andrea T. Fessler, Ralf Dieckmann, Lara Thieme, Mathias W. Pletz, Stefan Schwarz, Sascha Al Dahouk
Summary: Disinfection is crucial in reducing infections, but the excessive use of disinfectants may contribute to antimicrobial resistance. However, there is currently no consensus on this matter. The lack of standardized procedures and criteria to evaluate bacterial susceptibility to disinfectants makes data analysis challenging. Therefore, standardization and adaptation of evaluation concepts are urgently needed.
Article
Microbiology
Amelia S. Bhatnagar, Maria-Jose Machado, Logan Patterson, Karen Anderson, Rebecca L. Abelman, Allen Bateman, Angela Biggs, Porscha Bumpus-White, Bradley Craft, Mondraya Howard, Stephen P. LaVole, David Lonsway, Sarah Sabour, Annah Schneider, Paula Snippes-Vagnone, Michael Tran, David Torpey, Ann Valley, Christopher A. Elkins, Maria Karlsson, Allison C. Brown
Summary: In 2017, the CDC established the Antimicrobial Resistance Laboratory Network to improve detection of multidrug-resistant organisms. They evaluated a commercial broth microdilution panel and found that increasing the transfer volume can improve the accuracy of antimicrobial susceptibility testing, but also increases the rate of errors.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Review
Microbiology
Tanis C. Dingle, Johann Pitout
Summary: This article discusses the implementation of susceptibility testing and reporting of the newest beta-lactam/beta-lactamase inhibitors introduced to the North American antibiotic market, including ceftazidime-avibactam, meropenem-vaborbactam, and imipenem-relebactam.
JOURNAL OF CLINICAL MICROBIOLOGY
(2022)
Article
Microbiology
Ayesha Khan, William R. Miller, Dierdre Axell-House, Jose M. Munita, Cesar A. Arias
Summary: Enterococci are dangerous hospital pathogens with the potential of developing resistance to all antimicrobials. Accurate and feasible antimicrobial susceptibility testing methods are crucial in guiding the treatment of patients with enterococcal infections.
JOURNAL OF CLINICAL MICROBIOLOGY
(2022)
Article
Spectroscopy
Mohammad Javad Jafari, Mohsen Golabi, Thomas Ederth
Summary: The use of infrared attenuated total reflection (IR-ATR) provides a simple method for assessing bacterial susceptibility to antibiotics by monitoring bacterial metabolic activities. Principal component analysis can quickly discriminate antimicrobial resistance based on obtained spectra.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2024)
Article
Biochemical Research Methods
Yikang Xu, Dacheng Ren
Summary: The current phenotypic antibiotic susceptibility testing (AST) is time-consuming, while genotypic ASTs lack specific information at the phenotype level. To address this, researchers have developed a new electrochemical biosensor based on magnetically coupled LC sensors, which can rapidly detect bacterial susceptibility to antibiotics within 30 minutes.
JOURNAL OF BIOLOGICAL ENGINEERING
(2023)
Article
Microbiology
Xuejiao Hu, Yunhu Zhao, Peng Han, Suling Liu, Weijiang Liu, Cong Mai, Qianyun Deng, Jing Ren, Jiajie Luo, Fangyuan Chen, Xuefeng Jia, Jing Zhang, Guanhua Rao, Bing Gu
Summary: Multidrug-resistant (MDR) bacteria are a significant public health concern, and the current antibiotic susceptibility testing (AST) methods are time-consuming and can lead to delays in treatment and increased mortality. In this study, we developed a machine learning model using Acinetobacter baumannii as an example to explore a fast AST method using metagenomic next-generation sequencing (mNGS) data. The model accurately predicted antimicrobial resistance (AMR) signatures and antibacterial phenotypes, and the mNGS-based AST approach reduced reporting time significantly compared to culture-based AST methods. This model has the potential to be used for rapid genotypic AST and improve rational antimicrobial usage.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Veterinary Sciences
J. R. Fletcher, K. Y. Yuen, A. J. Stewart, A. C. Young, J. S. Gibson, O. A. James, C. E. Medina-Torres, B. M. Forde, A. Sole-Guitart
Summary: Chronic oroantral fistulae (OAF) with secondary sinusitis can occur in horses following repulsion of cheek teeth. This case report describes the successful treatment of a complicated OAF with concurrent MDR Escherichia coli infection using various therapeutic strategies. Whole-genome sequencing may be beneficial in guiding antimicrobial selection for MDR or XDR infections in the future.
AUSTRALIAN VETERINARY JOURNAL
(2022)
Article
Veterinary Sciences
Cleide H. Sprohnle-Barrera, Justine S. Gibson, Rochelle Price, Rikki M. Graham, Amy Jennison, Madeline R. Ricca, Rachel E. Allavena
Summary: This study reports a case of severe subcutaneous emphysema and multi-organ failure in a Siberian Husky dog. Pathological and genetic analysis revealed Clostridium perfringens type A as the causative pathogen with multiple virulence-related genes.
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
(2022)
Article
Infectious Diseases
Nicole Jacqueline Kalnins, Catriona Croton, Mark Haworth, Justine Gibson, Sarah Leonie Purcell, Allison Jean Stewart
Summary: This study investigated the antimicrobial prescribing habits of Australian veterinary clinics for dog-to-dog bite wounds. The results showed that antimicrobials were commonly prescribed for these wounds, with underdosing of a commonly used drug. The use of high-importance antimicrobials was associated with wound severity, year, and surgery.
Article
Veterinary Sciences
L. Awawdeh, C. Turni, J. L. Mollinger, J. Henning, R. N. Cobbold, D. J. Trott, J. S. Gibson
Summary: Avian colibacillosis is a major disease in poultry worldwide, causing significant mortality and economic losses. This study found a small reservoir of resistant E. coli in Australian commercial meat chickens, despite the absence of antibiotic use in the industry. Some of these resistant isolates carried resistance genes and signature mutations.
Article
Veterinary Sciences
Hester Rynhoud, Catriona Croton, Grace Henry, Erika Meler, Justine S. Gibson, Ricardo J. Soares Magalhaes
Summary: The study showed that dogs treated with oclacitinib required fewer courses of antimicrobials compared to dogs receiving other anti-pruritic treatments, indicating a potential sparing effect of oclacitinib on the prescription of antimicrobials for allergic skin diseases in dogs.
BMC VETERINARY RESEARCH
(2022)
Article
Genetics & Heredity
Lida Omaleki, Patrick J. Blackall, Thom Cuddihy, Rhys T. White, Jodi M. Courtice, Conny Turni, Brian M. Forde, Scott A. Beatson
Summary: This study investigates the outbreak dynamics of fowl cholera caused by Pasteurella multocida by using whole-genome sequencing and phylogenomic analysis. The analysis reveals a high degree of genetic diversity in the lipopolysaccharide outer core biosynthesis loci, indicating potential reasons for the repeated vaccination failures. The study also identifies potential phase variation mechanisms and emphasizes the importance of selecting appropriate vaccine strains based on the predicted LPS structure.
MICROBIAL GENOMICS
(2022)
Article
Veterinary Sciences
Nicole J. J. Kalnins, Justine S. Gibson, Allison J. J. Stewart, Catriona Croton, Sarah L. L. Purcell, Bandula Rajapaksha, Mark Haworth
Summary: Dog-to-dog bite wounds are common, but studies on antimicrobial usage patterns are limited. This retrospective study evaluated 1526 dog bite events from 1999 to 2019 in three Australian university clinics and found that amoxicillin-clavulanic acid was the most commonly prescribed antimicrobial. The study also found an association between wound severity and the use of high-importance antimicrobials. Routine susceptibility testing is recommended to optimize antimicrobial stewardship.
JOURNAL OF VETERINARY INTERNAL MEDICINE
(2022)
Article
Multidisciplinary Sciences
Tasneem Imam, Justine S. Gibson, Suman Das Gupta, Mohammad Foysal, Shetu B. Das, Md Ahasanul Hoque, Guillaume Fournie, Joerg Henning
Summary: Adapting the Social Cognitive Theory framework, a cross-sectional study was conducted on 137 commercial chicken farms in Bangladesh to investigate factors influencing farmers' behavior towards the application of antimicrobials. The study found that almost all farmers used antimicrobials for treating poultry diseases, with a significant percentage also using them for healthy growth and increased production. The inappropriate usage of antimicrobials was primarily driven by short-term goals, while farmers' perception of control over antimicrobial administration had a marginal influence. The study suggests the need for targeted education programs to reduce antimicrobial application in poultry flocks.
SCIENTIFIC REPORTS
(2023)
Article
Agriculture, Dairy & Animal Science
Suzanne Suk Kwan Tso, Ellie Leister, Claire Rebecca Sharp, Jane Heller, Justine S. Gibson
Summary: This study describes the clinical course, culture and susceptibility profiles of dogs and cats mechanically ventilated for tick paralysis. The majority of these cases had concurrent evidence of aspiration pneumonia. Appropriate use of antimicrobials improved outcome. Clinicians should be aware of the development of bacterial pneumonia and select antimicrobials based on culture and susceptibility testing.
Article
Veterinary Sciences
Helen Owen, Emily Jones, Carla Kowald, Sebastian Hand, Michael McGowan, Rowland Cobbold, Tamsin S. Barnes, Justine S. Gibson, Shahab Ranjbar, Chiara Palmieri, Rachel Allavena
Summary: This study aimed to create a post-mortem data collection tool for evaluating liver diseases in Australian beef cattle and to analyze the pathological findings in both normal and condemned livers. The study developed an efficient tool for rapid assessment of large numbers of cattle livers, allowing thorough investigation into the impact of liver disease on beef production.
RESEARCH IN VETERINARY SCIENCE
(2023)
Article
Multidisciplinary Sciences
Sara Horsman, Erika Meler, Deirdre Mikkelsen, John Mallyon, Hong Yao, Ricardo J. Soares Magalhaes, Justine S. S. Gibson
Summary: This study investigates the associations between Staphylococcus spp. infections and nasal microbiota in a canine animal shelter population. It reveals that shelter dogs with dermatological conditions have different nasal microbial communities compared to those without dermatological conditions, with decreased diversity and abundance. The study emphasizes the importance of exploring decolonization and probiotic therapies for restoring the nasal microbiota.
SCIENTIFIC REPORTS
(2023)
Article
Veterinary Sciences
Doan Hoang Phu, Ruethai Narinthorn, Nguyen Thi Nhung, Niwat Chansiripornchai, Patrick J. Blackall, Conny Turni, Juan Carrique-Mas, Thotsapol Thomrongsuwannakij
Summary: Chicken in southern Thailand has different prevalence and resistance patterns of Campylobacter. Commercial broilers exhibit higher resistance to quinolones and lower resistance to macrolides and tetracycline compared to native chickens. Resistance patterns are more consistent within farms, and commercial broilers show higher homogeneity. Phenotypic and genotypic resistance have partial agreement among isolates.
Article
Veterinary Sciences
Jinnat Ferdous, Md Helal Uddin, Rashed Mahmud, Mathew Hennessey, Abdullah Al Sattar, Suman Das Gupta, Justine S. Gibson, Robyn Alders, Joerg Henning, Guillaume Fournie, Md. Ahasanul Hoque
Summary: Bangladesh's commercial poultry production is rapidly growing, primarily due to the perception that processed poultry provides safer food than traditional live bird markets. However, this study found a lack of consistency in quality control and meat traceability within the dressed and processed poultry production and distribution network (PDN). This raises concerns about the safety of dressed poultry products and the potential increase in food-borne disease risks.
VETERINARY RESEARCH COMMUNICATIONS
(2023)
Correction
Veterinary Sciences
Jinnat Ferdous, Helal Uddin, Rashed Mahmud, Mathew Hennessey, Abdullah Al Sattar, Suman Das Gupta, Justine S. Gibson, Robyn Alders, Joerg Henning, Guillaume Fournie, Ahasanul Hoque
VETERINARY RESEARCH COMMUNICATIONS
(2023)
Article
Marine & Freshwater Biology
Alexandra M. McGowan, Jennifer M. Seddon, Janet M. Lanyon, Nicholas Clark, Justine S. Gibson
Summary: Bacterial isolates cultured from the fresh faeces of dugongs in Queensland, Australia, showed resistance to antibiotics such as penicillin and trimethoprim, and contained various resistance and virulence genes. This highlights the role that dugongs can play as a sentinel species for antimicrobial resistance in coastal waters.
