Article
Multidisciplinary Sciences
Alex J. Vecchio, Sewwandi S. Rathnayake, Robert M. Stroud
Summary: The study reveals the molecular and structural basis of how CpE targets susceptible claudins, as well as differences in the primary CpE receptors in mice and humans due to sequence changes in the target motif.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Multidisciplinary
Man Kyu Shim, Jinhee Na, In Kyung Cho, Eun Hyang Jang, Jooho Park, Sangmin Lee, Jong-Ho Kim
Summary: The study developed a claudin-4-targeting drug carrier that efficiently accumulated in pancreatic cancer tissues, suppressed tumor growth with minimal toxicity to normal tissues. This claudin-4-targeting drug carrier may represent a promising new approach for treating pancreatic cancer.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Microbiology
Yosra Ben Cheikh, Marie Agnes Travers
Summary: Studies on marine epizootics often focus on a single pathogen, but this paradigm is not always sufficient to explain the disease. This study found that Vibrio splendidus strains were associated with mortality in Mytilus edulis and that the structure and function of the mussel microbiota were affected by Vibrio exposure.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Food Science & Technology
Liyou Dong, Renata M. C. Ariens, Antoine H. P. America, Aman Paul, Teun Veldkamp, Jurriaan J. Mes, Harry J. Wichers, Coen Govers
Summary: The study investigated the antimicrobial capacity of insect-derived digest against C. perfringens, with fractions containing black soldier fly larvae protein showing significant inhibition of bacterial growth. It also found that exposure to the digest resulted in a significant reduction in abundance and diversity of healthy microbiota, mainly affecting Firmicutes and Proteobacteria, while increasing SCFA secretion. This suggests that black soldier fly larvae protein could be a promising additional tool in combating C. perfringens infection.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Review
Biology
Floriana Gernone, Annamaria Uva, Marco Silvestrino, Maria Alfonsa Cavalera, Andrea Zatelli
Summary: This review discusses the possible role of gut microbiota in the etiopathogenesis of canine idiopathic epilepsy, summarizing recent clinical and preclinical studies supporting the connection between microbiota and epilepsy via the gut-brain axis.
Article
Medicine, Research & Experimental
Yinghua Ma, David Sannino, Jennifer R. Linden, Sylvia Haigh, Baohua Zhao, John B. Grigg, Paul Zumbo, Friederike Duendar, Daniel Butler, Caterina P. Profaci, Kiel Telesford, Paige N. Winokur, Kareem R. Rumah, Susan A. Gauthier, Vincent A. Fischetti, Bruce A. McClane, Francisco A. Uzal, Lily Zexter, Michael Mazzucco, Richard Rudick, David Danko, Evan Balmuth, Nancy Nealon, Jai Perumal, Ulrike Kaunzner, Ilana L. Brito, Zhengming Chen, Jenny Z. Xiang, Doron Betel, Richard Daneman, Gregory F. Sonnenberg, Christopher E. Mason, Timothy Vartanian
Summary: Multiple sclerosis (MS), a complex disease of the CNS, has been linked to gut dysbiosis. This study found that individuals with MS were more likely to have epsilon toxin-producing strains of C. perfringens in their gut microbiomes compared to healthy controls. These strains were able to produce functional epsilon toxin and had genetic characteristics similar to highly conjugative plasmids. In an animal model of MS, epsilon toxin could substitute for pertussis toxin and induced demyelination in various regions of the CNS, similar to lesions seen in MS patients.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Food Science & Technology
Jihong Li, Eric Mi, Arhat Pradhan, Bruce A. McClane
Summary: This study investigated the influence of NanR on c-cpe strains. In sporulation medium, the nanR null mutants showed lower sialidase activity, sporulation, and CPE production. In vegetative medium, the nanR null mutants produced more spores. These results suggest that NanR plays a crucial role in the regulation of c-cpe strains.
Review
Oncology
Amita R. Banga, Peace Odiase, Kartik Rachakonda, Amar P. Garg, Samuel E. Adunyah, Girish Rachakonda
Summary: This article discusses the potential value of the interaction between Clostridium perfringens Enterotoxin (CPE) and Claudin-4 in the treatment of breast cancer metastasis to the brain, and introduces the important role of Claudin-4 in cell trafficking and disease development.
Article
Agriculture, Dairy & Animal Science
Javier Arenas-Montes, Pablo Perez-Martinez, Cristina Vals-Delgado, Juan Luis Romero-Cabrera, Magdalena P. Cardelo, Ana Leon-Acuna, Gracia M. Quintana-Navarro, Juan F. Alcala-Diaz, Jose Lopez-Miranda, Antonio Camargo, Francisco Perez-Jimenez
Summary: Pet ownership may reduce the risk of metabolic syndrome and obesity, with dog ownership showing more significant benefits. Having pets can alter the gut microbiota and contribute to the prevention of cardiometabolic diseases.
Article
Veterinary Sciences
Sergio Alvarez-Perez, Blanca Anega, Jose L. Blanco, Marta Hernandez, Marta E. Garcia
Summary: This study demonstrates the high effectiveness of fidaxomicin against C. perfringens strains from dogs and cats. Although fidaxomicin is considered critically important and not yet licensed for veterinary use, the results provide useful baseline data for tracking the emergence of fidaxomicin-resistant C. perfringens strains in the veterinary setting.
BMC VETERINARY RESEARCH
(2023)
Article
Microbiology
Mauricio A. Navarro, Jihong Li, Juliann Beingesser, Bruce A. McClane, Francisco A. Uzal
Summary: NanI can significantly enhance the activity of C. perfringens enterotoxin (CPE) in the presence of mucus, leading to increased cytotoxicity and damage to intestinal mucosa.
Article
Nutrition & Dietetics
Zipeng Jiang, Wentao Li, Weifa Su, Chaoyue Wen, Tao Gong, Yu Zhang, Yizhen Wang, Mingliang Jin, Zeqing Lu
Summary: This study aimed to investigate the protective effects of Bacillus amyloliquefaciens (BA40) against Clostridium perfringens infection in mice. The results demonstrated that BA40 treatment alleviated intestinal structure damage, reduced inflammatory responses, and restored intestinal microbiota imbalance induced by the C. perfringens infection. Additionally, BA40 treatment also influenced metabolic pathways, such as purine metabolism, 2-oxocarboxylic acid metabolism, and starch and sucrose metabolism. Overall, BA40 effectively protected mice from C. perfringens infection.
FRONTIERS IN NUTRITION
(2021)
Article
Agriculture, Dairy & Animal Science
Fangshen Guo, Fangyuan Wang, Haiyan Ma, Zhouzheng Ren, Xiaojun Yang, Xin Yang
Summary: The study reveals that dietary deoxynivalenol and Clostridium perfringens challenge have harmful effects on the jejunal health of broiler chickens, affecting mucosal expression and intestinal microbiota composition.
Article
Food Science & Technology
Thea Neumann, Maren Krueger, Jasmin Weisemann, Stefan Mahrhold, Daniel Stern, Martin B. Dorner, Cecile Feraudet-Tarisse, Christopher Poehlmann, Katharina Schulz, Ute Messelhaeusser, Dagmar Rimek, Frank Gessler, Thomas Elssner, Stephanie Simon, Andreas Rummel, Brigitte G. Dorner
Summary: The study successfully identified nine specific mAbs targeting CPE and showed their high affinity in detecting CPE. Through ELISAs, CPE can be detected specifically, which is useful for diagnosing diarrhea in clinical laboratories.
Article
Microbiology
Xin Feng, Tonghao Li, Hui Zhu, Lidan Liu, Shengqun Bi, Xiaolin Chen, Huihua Zhang
Summary: This study investigated the changes in ileal microbiota of yellow feather broilers under Clostridium perfringens and/or Eimeria challenge. The results showed that co-infection with Eimeria significantly decreased the diversity of ileal microbiota, and C. perfringens and Eimeria challenge also decreased the relative abundance of beneficial bacteria.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Melanie Werner, Jan S. Suchodolski, Jonathan A. Lidbury, Jorg M. Steiner, Katrin Hartmann, Stefan Unterer
Summary: The study found that fecal cultures were ineffective in distinguishing between dogs with chronic diarrhea and healthy dogs, and there was a high level of variability in culture results among different laboratories.
JOURNAL OF VETERINARY INTERNAL MEDICINE
(2021)
Article
Veterinary Sciences
Linda Toresson, Joerg M. Steiner, Eva Spodsberg, Gunilla Olmedal, Jan S. Suchodolski, Jonathan A. Lidbury, Thomas Spillmann
Summary: This study showed that oral cobalamin supplementation is a potential alternative to parenteral supplementation in dogs with exocrine pancreatic insufficiency (EPI), significantly increasing serum cobalamin concentrations.
VETERINARY JOURNAL
(2021)
Article
Veterinary Sciences
Carolyn E. Arnold, Rachel Pilla, M. Keith Chaffin, Jessica L. Leatherwood, Tryon A. Wickersham, Todd R. Callaway, Sara D. Lawhon, Jonathan A. Lidbury, Joerg M. Steiner, Jan S. Suchodolski
Summary: This study examined the influence of various factors on the fecal microbiota of healthy horses, revealing that the amount of grain in the diet and colitis had significant effects. Horses with AAD showed more severe dysbiosis compared to those with Salmonella infection.
JOURNAL OF VETERINARY INTERNAL MEDICINE
(2021)
Review
Veterinary Sciences
Jan S. Suchodolski
Summary: The gut microbiome plays a crucial role in maintaining immune and metabolic functions, with impact on intestinal and overall organ health. Dysbiosis concept continues to evolve, with molecular tools becoming the standard for microbiome analysis, though challenges in standardization and result consistency persist. The Dysbiosis Index is a useful tool for assessing microbiome status in clinical patients.
VETERINARY CLINICAL PATHOLOGY
(2022)
Article
Veterinary Sciences
Sarah E. Cocker, Yuri A. Lawrence, Jonathan A. Lidbury, Jan S. Suchodolski, Jorg M. Steiner, Keith P. Richter
Summary: Long-term administration of supraphysiologic doses of GCs significantly increases serum cPLI concentrations in sick dogs, although the change is small and often clinically insignificant, it may pose a clinical interpretation dilemma in some dogs.
VETERINARY CLINICAL PATHOLOGY
(2022)
Article
Veterinary Sciences
I. L. Oikonomidis, K. Theodorou, E. Papaioannou, P. G. Xenoulis, K. K. Adamama-Moraitou, J. M. Steiner, M. Kritsepi-Konstantinou, J. S. Suchodolski, T. Rallis, N. Soubasis
Summary: This study investigated the changes in thyroid hormone concentrations in dogs with canine parvoviral enteritis during hospitalization and found an association between thyroid hormones and prognosis, as well as the development of systemic inflammatory response syndrome.
VETERINARY JOURNAL
(2021)
Article
Immunology
K. G. Kokkinaki, M. N. Saridomichelakis, L. Leontides, M. E. Mylonakis, A. O. Konstantinidis, J. M. Steiner, J. S. Suchodolski, P. G. Xenoulis
Summary: This study investigated the epidemiological, clinical, and clinicopathologic aspects of FeLV and FIV infections in different populations of cats in Greece. Various factors and clinical signs were found to be associated with FeLV and FIV infections, emphasizing the importance of testing all sick cats, especially those in high-risk groups.
COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES
(2021)
Article
Immunology
M. K. Chatzis, D. Kasabalis, J. M. Steiner, M. N. Saridomichelakis, J. S. Suchodolski, P. G. Xenoulis
Summary: The study found that dogs with leishmaniosis had significantly lower serum cobalamin concentrations before treatment compared to clinically healthy dogs, and there was an increase in serum cobalamin concentrations during treatment.
COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES
(2021)
Article
Veterinary Sciences
Elisabeth Skotnitzki, Jan S. Suchodolski, Kathrin Busch, Melanie Werner, Yury Zablotski, Bianca D. Ballhausen, Felix Neuerer, Stefan Unterer
Summary: The study reported a higher prevalence of signs of chronic GI disease in dogs after an episode of acute hemorrhagic diarrhea (AHD), possibly due to severe intestinal mucosal damage and associated barrier dysfunction triggering chronic GI disease later in life.
JOURNAL OF VETERINARY INTERNAL MEDICINE
(2022)
Article
Veterinary Sciences
Panagiotis G. Xenoulis, Romy M. Heilmann, Eva M. Stavroulaki, Denise S. Riggers, Laura J. Gneipel, Jan S. Suchodolski, Jorg M. Steiner
Summary: This study investigated the association between HTGL and IR as well as the subclinical inflammatory phenotype in Miniature Schnauzers. It was found that HTGL in these dogs was associated with increased serum calprotectin concentrations, and dietary intervention led to a decrease in serum insulin concentrations.
JOURNAL OF VETERINARY INTERNAL MEDICINE
(2022)
Article
Veterinary Sciences
Chee-Hoon Chang, Jonathan A. Lidbury, Jan S. Suchodolski, Joerg M. Steiner
Summary: This study found that oral supplementation is an alternative to injectable supplementation for cobalamin supplementation in dogs with hypocobalaminemia caused by chronic enteropathy or exocrine pancreatic insufficiency.
JOURNAL OF VETERINARY INTERNAL MEDICINE
(2022)
Article
Veterinary Sciences
F. C. Droes, J. S. Suchodolski, J. M. Steiner, J. A. Lidbury
Summary: This study aimed to estimate the prevalence and describe the clinical and laboratory data of dogs with chronic hepatitis and portal vein thrombosis and splanchnic venous thrombosis. The study found that portal vein and splanchnic venous thrombosis are potentially serious complications that were identified in a relatively low proportion of dogs with chronic hepatitis.
JOURNAL OF SMALL ANIMAL PRACTICE
(2023)
Article
Veterinary Sciences
Michael Hung, Justin Heinz, Jorg M. Steiner, Jan Suchodolski, Jonathan Lidbury
Summary: This study evaluated serum cobalamin and methylmalonic acid concentrations in juvenile dogs with parvoviral enteritis or nonparvoviral acute enteropathy. The results showed that juvenile dogs with parvoviral enteritis or nonparvoviral acute enteropathy had lower serum cobalamin concentrations than healthy juvenile dogs, but cellular cobalamin deficiency was not apparent based on serum MMA concentrations.
JOURNAL OF VETERINARY INTERNAL MEDICINE
(2023)
Article
Veterinary Sciences
Rae Mcatee, Sarah M. Schmid, M. Katherine Tolbert, Scott Hetzel, Jan S. Suchodolski, Jessica C. Pritchard
Summary: In healthy dogs, concurrent administration of a probiotic does not mitigate the adverse effects caused by esomeprazole administration. However, it does lead to an increase in fecal dysbiosis index.
JOURNAL OF VETERINARY INTERNAL MEDICINE
(2023)
Article
Veterinary Sciences
Sharon Kuzi, Soha Zgairy, Barbara A. Byrne, Jan Suchodolski, Sondra C. Turjeman, So Young Park, Itamar Aroch, Mike Hong, Omry Koren, Eran Lavy
Summary: This study investigated the fecal microbiome features in dogs with giardiasis and found that the fecal microbial composition of symptomatic and asymptomatic dogs with giardiasis is similar. Giardiasis is not characterized by prominent dysbiosis based on fecal dysbiosis index (DI).
JOURNAL OF VETERINARY INTERNAL MEDICINE
(2023)