期刊
VETERINARY MICROBIOLOGY
卷 171, 期 1-2, 页码 66-73出版社
ELSEVIER
DOI: 10.1016/j.vetmic.2014.03.009
关键词
Rotavirus; Novel genotype; Adult cow diarrhea; Viral metagenomics; Japan
资金
- Ministry of Health, Labour and Welfare, Japan [H24-shinkou-ippan-005]
- Grants-in-Aid for Scientific Research [24780294, 25305010, 24580430] Funding Source: KAKEN
There are few reports describing diarrhea of adult cattle caused by group A rotaviruses. Here, we report the identification of a novel bovine group A rotavirus from diarrhea of adult cows. A group A rotavirus was detected from an epizootic outbreak of diarrhea in adult cows with a decrease in milk production in Japan in 2013. The comprehensive genomic analyses from fecal samples by viral metagenomics using a next-generation sequencer revealed that it had an unreported genotype combination G15P[14]. The genome constellation of this strain, namely, RVA/Cow-wt/JPN/Tottori-SG/2013/G15P[14]] was G15-P[14]-12-R2-C2-M2-A3-N2-T6-E2-H3 representing VP7-VP4-VP6-VP1-VP2-VP3-NSP1-NSP2-NSP3-NSP4-NSP5, respectively. Each gene segment of Tottori-SG was most closely related to Japanese bovine group A rotaviruses suggesting that Tottori-SG might have derived from multiple reassortment events from group A rotavirus strains circulating among Japanese cattle. No other diarrhea pathogen of adult cattle was detected by routine diagnosis and metagenomics. Viral metagenomics, using a next-generation sequencer, is useful to characterize group A rotaviruses from fecal samples and offers unbiased comprehensive investigations of pathogen. (C) 2014 Elsevier B.V. All rights reserved.
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