Review
Microbiology
Gang Zhou, Qian Wang, Yingsi Wang, Xia Wen, Hong Peng, Ruqun Peng, Qingshan Shi, Xiaobao Xie, Liangqiu Li
Summary: This review examines the properties and interactions of five common outer membrane proteins (Omps), including OmpA, OmpC, OmpF, OmpW, and OmpX, in relation to porin-mediated permeability in gram-negative bacteria. It also highlights the discovered regulatory characteristics and identified molecular mechanisms in antibiotic penetration through Omps. Uncovering the functional properties of Omps will pave the way for investigating effective agents or approaches that use Omps as targets to eliminate resistant gram-negative bacteria.
Review
Microbiology
Brijeshwar Singh, Surbhi Jaiswal, Prashant Kodgire
Summary: The indiscriminate use of antibiotics has led to the emergence of unmanageable antibiotic-resistant bacteria. Vibrio spp. is a gram-negative enteric pathogenic group with more than 100 species infecting humans and fish. It is divided into two groups, one causing cholera and the other causing non-cholera or vibriosis infections. Preventative measures such as vaccinations need to be developed to control Vibrio infections and reduce reliance on antibiotics, especially with the emergence of multi-drug resistant strains. This review aims to provide comprehensive information on Vibrio spp. outer membrane proteins and vesicles, including their structure, function, and immunoprotective role.
CRITICAL REVIEWS IN MICROBIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Federica Dell'Annunziata, Veronica Folliero, Rosa Giugliano, Anna De Filippis, Cristina Santarcangelo, Viviana Izzo, Maria Daglia, Massimiliano Galdiero, Carla Renata Arciola, Gianluigi Franci
Summary: Outer membrane vesicles (OMVs) released by Gram-negative bacteria are rounded nanostructures that can encapsulate biologically active toxins and virulence factors, and have been recently found to mediate horizontal gene transfer processes. Little is known about the packaging, release, and transfer mechanisms of OMVs, but they play an important role in HGT processes in bacteria.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Ren-Hsuan Ku, Li-Hua Li, Yi-Fu Liu, En-Wei Hu, Yi-Tsung Lin, Hsu-Feng Lu, Tsuey-Ching Yang
Summary: This study investigated the mechanism of decreased oxidative stress tolerance mediated by Delta ompA(299-356) mutant and revealed the regulatory network between Delta ompA(299-356)-rpoE-ompO and rpoE-rpoN.
MICROBIOLOGY SPECTRUM
(2023)
Review
Biochemistry & Molecular Biology
Sofia Combo, Sergio Mendes, Kaare Magne Nielsen, Gabriela Jorge da Silva, Sara Domingues
Summary: Outer membrane vesicles (OMV) released from Gram-negative bacteria can carry molecules with antibacterial activity, making them potential tools for delivering antimicrobial agents.
Article
Microbiology
Janine H. Peterson, Matthew Thomas Doyle, Harris D. Bernstein
Summary: Several antibacterial compounds have been found to inhibit BAM function through different mechanisms, affecting the biogenesis of outer membrane proteins in Gram-negative bacteria. These findings not only provide important insights for developing novel broad-spectrum antibiotics, but also valuable tools for future studies on BAM function.
Review
Chemistry, Medicinal
Charlotte M. J. Wesseling, Nathaniel I. Martin
Summary: New approaches targeting Gram-negative bacteria are crucial in combating antibiotic resistance. This review focuses on peptide-based and small organic compounds that disrupt the outer membrane of Gram-negative bacteria and synergize with clinically used antibiotics. The review also assesses the hemolytic activity of these compounds and their selectivity towards Gram-negative bacteria.
ACS INFECTIOUS DISEASES
(2022)
Article
Immunology
Hidetada Hirakawa, Kazutomo Suzue, Haruyoshi Tomita
Summary: The Tol/Pal system is a set of protein complexes produced by Gram-negative bacteria, playing essential roles in colicin uptake, pathogenesis, and cell growth. It is proposed as a potential target for antimicrobial chemotherapy and vaccine development due to its immunogenicity and ability to stimulate immune responses.
Article
Microbiology
Claire Maher, Ram Maharjan, Geraldine Sullivan, Amy K. Cain, Karl A. Hassan
Summary: This study investigated the significance of a primary amine group as a chemical feature that promotes the uptake and accumulation of compounds in the Gram-negative model organism Escherichia coli. The results showed that the primary amine promotes passage through the outer membrane porin OmpF and active efflux plays a major role in resistance.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Claire Maher, Ram Maharjan, Geraldine Sullivan, Amy K. Cain, Karl A. Hassan
Summary: Gram-negative bacteria have low permeability in their cell envelopes, making it difficult for antibiotic development. This study aimed to design new antibiotics that can breach this barrier by examining the specific components of the cell envelope. The findings showed that ampicillin has better permeability than benzylpenicillin through the outer membrane porin OmpF. Disruption of the outer membrane compromised the fitness of bacteria under benzylpenicillin exposure, while it had a smaller effect under ampicillin treatment. Disruption of genes encoding the AcrAB-TolC efflux system had a detrimental effect on survival under both antibiotics, particularly ampicillin.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biochemistry & Molecular Biology
Miran Tang, Deyi Zhao, Sichen Liu, Xiaotuan Zhang, Zhuocheng Yao, Hule Chen, Cui Zhou, Tieli Zhou, Chunquan Xu
Summary: This study aimed to investigate the mechanisms of synergy between pentamidine and antibiotics against Gram-negative bacteria. The results showed that pentamidine strongly synergized with rifampicin, linezolid, and tetracycline, but not with vancomycin. Pentamidine increased membrane permeability, dissipated membrane proton motive force, and inactivated the efflux pump, leading to intracellular accumulation of antibiotics and bacterial destruction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Ron Solan, Joana Pereira, Andrei N. Lupas, Rachel Kolodny, Nir Ben-Tal
Summary: Research has identified many proteins combining multiple beta barrels within a single chain, with combinations that include eight-stranded barrels prevailing. These multibarrels seem to result from independent, lineage-specific fusion and amplification events. The absence of universally conserved multibarrels in bacteria with an outer membrane, along with their frequent de novo genesis, suggests that their functions are beneficial rather than essential in specific environments.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Analytical
Yi-Fei Qin, Xin-Yu Lu, Zheng Shi, Qian-Sheng Huang, Xiang Wang, Bin Ren, Li Cui
Summary: Pathogenic bacterial infections and increasing antimicrobial resistance pose a major threat to human health. This study developed a label-free Raman spectroscopy combined with deep learning model for the rapid detection and identification of pathogen-derived extracellular vesicles (EVs). The platform enables accurate identification of EVs at different levels and provides insights into their biogenesis process, spectral markers, and bacterial origin. This will facilitate rapid diagnosis and timely treatment of bacterial infections.
ANALYTICAL CHEMISTRY
(2022)
Review
Immunology
Shuming Wang, Jiayi Guo, Yang Bai, Cai Sun, Yanhao Wu, Zhe Liu, Xiaofei Liu, Yanfeng Wang, Zhigang Wang, Yongmin Zhang, Huifang Hao
Summary: This paper reviews the applications of bacterial outer membrane vesicles in tumor vaccines and their interaction with host cells.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Engineering, Biomedical
Wenlong Huang, Lingxi Meng, Yuan Chen, Zaiquan Dong, Qiang Peng
Summary: Antibiotic therapy is important, but its improper use and emergence of drug resistance have reduced its effectiveness. Therefore, developing more potent antimicrobial therapies is crucial. Outer membrane vesicles (OMVs) secreted by Gram-negative bacteria have been used as active agents, drug carriers, and vaccine adjuvants, showing great potential.
ACTA BIOMATERIALIA
(2022)