期刊
VETERINARY MICROBIOLOGY
卷 165, 期 3-4, 页码 378-383出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetmic.2013.04.020
关键词
E. coli; AHL; Quorum Sensing; Flagella
资金
- Chinese National Science Foundation [31072136, 30771603, 31270171]
- Jiangsu High Education Key Basic Science Foundation [08KJA230002]
- Genetically Modified Organisms Technology Major Project of China [2009ZX08006-004B]
To investigate the effect of the Quorum Sensing (QS)-I system on the expression of virulence factors in Shiga toxin producing and verotoxin-producing Escherichia coli (STEC and VTEC), the yenI gene from Yersinia enterocolitica was cloned into E. coli F18ab 107/86. Recombinant E. coli transformed with yenI produced acyl-homoserine lactone synthase (AHL), as measured using cross-streaking assays with the reporter biosensor strain Chromobacterium violaceum CV026. The AI-1 positive recombinant F18ab E. coli exhibited impaired expression of flagella, decreased motility, reduced biofilm formation and AI-2 production, as well as attenuated adherence and invasion on IPEC-J2 cells. This study provides new insights to the crucial function of AI-1 in regulating STEC virulence. (c) 2013 Published by Elsevier B.V.
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