Article
Fisheries
Chang Wei, Chaonan Xu, Yuyu Sun, Jinzhou Li, Motohiko Sano, Qiang Li
Summary: This study found that CyHV-2 can establish persistent or latent infection in asymptomatic farmed gibel carp, and different viral mRNA expression patterns were observed in different individuals. This indicates that apparently healthy gibel carp can also be a source of infection.
Article
Immunology
Peng Chen, Mingming Zhang, Yichan Zhang, Jun Li, Xihe Wan, Tingli Lv, Yiyue Chen, Zhigang Zhao, Zhihao Ma, Zhu Zhu, Lihua Chen, Zhen Li, Zisheng Wang, Guo Qiao
Summary: This study analyzed the impact of Cyprinid herpesvirus 2 (CyHV-2) infection on the gut microbiota and metabolite composition of gibel carp. The results showed that CyHV-2 infection significantly altered the gut microbiota and metabolite profiles, leading to an increase in the opportunistic pathogen Aeromonas. Furthermore, modulation of tryptophan metabolism affected the abundance of Aeromonas, the transcription of antiviral cytokines, and the infection level of CyHV-2.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Mingyang Xue, Miao Fu, Mengwei Zhang, Chen Xu, Yan Meng, Nan Jiang, Yiqun Li, Wenzhi Liu, Yuding Fan, Yong Zhou
Summary: This study investigated the defensive ability of gibel carp exposed to aflatoxin B1 (AFB1) against cyprinid herpesvirus 2 (CyHV-2) infection. The research found that AFB1 exposure increased mortality and viral load in the fish, induced oxidative stress, and down-regulated the expression of Nrf2, IRF3, and IFN1 while up-regulating caspase-1. Additionally, AFB1 exposure affected the intestinal microbiota composition by decreasing diversity and richness and altering the abundance of specific bacteria. These findings suggest that AFB1 may increase susceptibility to CyHV-2 infection in gibel carp and pose a threat to ecological safety in aquatic environment.
Article
Fisheries
Fei Shen, Yufei Zhai, Xiaomi Zhang, Hao Wang, Liqun Lu
Summary: This study tested the inhibitory activity of (-)-Epigallocatechin-3-gallate (EGCG) against the main pathogens Aeromonas hydrophila and cyprinid herpesvirus 2 (CyHV-2) in gibel carp. The results showed that EGCG can effectively inhibit the growth of these pathogens and reduce their load in gibel carp.
Article
Agriculture, Dairy & Animal Science
Wanjie Cai, Lele Fu, Cui Liu, Linyue He, Haokun Liu, Dong Han, Xiaoming Zhu, Yunxia Yang, Junyan Jin, Shouqi Xie
Summary: This study investigated the effect of D-ribose on the flesh quality of gibel carp. The results showed that D-ribose supplementation improved the nutritional value, texture, and flavor of the fish. It enhanced the water-holding capacity and hardness of the flesh, facilitated the deposition of amino acids and fatty acids, and activated purine metabolism pathways.
Article
Fisheries
Yuyu Sun, Chaonan Xu, Hao Wang, Guo Qiao, Zhenhui Wang, Zheng Li, Qiang Li, Chang Wei
Summary: An attenuated strain of CyHV-2 G-RP7 was obtained by subculture on RyuF-2 cells and GiCF cells. Gibel carp showed no clinical symptoms when immersed or injected intraperitoneally with G-RP7 strain. The protection rates of G-RP7 by immersion and intraperitoneal injection were 92% and 100%, respectively.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Lu Chen, Yan'ou Yang, Muhammad Rizwan, Ding'ding Yue, Ibrar Muhammad Khan, Feng Yao, Juan Zhong, Shuang'jie Huang
Summary: The research revealed that high stocking density negatively impacted the growth of gibel carp, with the D-1/2 group achieving complete compensatory growth, while the D-1/4 and D-1/8 groups only partially compensated for the stress.
AQUACULTURE RESEARCH
(2021)
Article
Fisheries
Yipeng Gu, Kai Chen, Bingwen Xi, Jun Xie, Xuwen Bing
Summary: Paeonol exhibits therapeutic effects on Carassius auratus gibelio infected by pathogenic bacteria Aeromonas hydriphila, improving survival rate, attenuating oxidative damage, inflammation, tissue lesions, and enhancing immunity.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Bing-Yao Sun, Hai-Yan Kou, Pan -Yang Jian, Ling-Jiao Kong, Jie Fang, Peng-Kun Meng, Kang Wu, Cai-Gen Yang, Gang Yang, Xue-Hong Song
Summary: This study aimed to develop a specific IgY against CyHV-2 infection in gibel carp. Specific IgY was generated by immunizing laying hens with a recombinant D4ORFs protein and processed into spray-dried egg powder. The results showed that gibel carp receiving D4ORFs IgY had higher survival rates and lower viral load, as well as improved health and enhanced antiviral defense.
Article
Biochemistry & Molecular Biology
Yipeng Gu, Kai Chen, Bingwen Xi, Jun Xie, Xuwen Bing
Summary: The study showed that paeonol could alleviate LPS-induced liver oxidative stress and inflammation in gibel carp by reducing abnormal plasma biochemical indexes, restoring antioxidant enzyme activity, inhibiting the expression of inflammatory-related genes, and alleviating liver tissue damage.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2022)
Article
Fisheries
Wenping Ren, Xiaoyi Pan, Caijiao Dai, Tiancheng Shu, Lijuan Li, Junfa Yuan
Summary: The study found that CyHV-2 infection triggered secondary bacterial infections, with mortality depending on pathogen burden. Evaluation of the integrity of the intestinal mucosal barrier and transcriptome analysis showed disruption of gut homeostasis after CyHV-2 infection in crucian carp.
Article
Chemistry, Applied
Wanjie Cai, Haokun Liu, Linyue He, Lele Fu, Dong Han, Xiaoming Zhu, Junyan Jin, Yunxia Yang, Shouqi Xie
Summary: This study examined the effects of exercise training (ET) combined with a high-fat diet (HFD) on flesh quality. It was found that HFD increased muscular fat content but reduced hardness, flexibility, and adhesiveness, while ET decreased fat content but increased flesh water holding capacity, hardness, and stickiness. The combination of ET and HFD improved the aroma of flesh by enhancing the content of key flavor compounds. This research provides a new strategy for improving the quality of cultured fish flesh.
Article
Fisheries
Ke Ji, Juyun He, Hualiang Liang, Mingchun Ren, Xianping Ge, Karthik Masagounder
Summary: The study investigated the response of gibel carp to dietary lysine levels in zero fish meal diets and found that optimal lysine levels significantly improved growth performance and nutrient absorption in gibel carp. However, excess lysine levels may lead to abnormal physiological indicators, highlighting the importance of controlling dietary lysine levels.
AQUACULTURE NUTRITION
(2021)
Article
Fisheries
Yan Zhou, Yan Li, Luo Lei, Xingxing Deng, Yuting Duan, Suxing Fu, Jingsen Zhang, Dengyue Yuan, Chaowei Zhou, Wenping He
Summary: This study cloned and characterized the coding DNA sequence (CDS) of the MC4R gene in gibel carp and investigated its role in food regulation. The results showed that CgMC4R was predominantly expressed in the hypothalamus and its expression responded to feeding and fasting conditions.
AQUACULTURE INTERNATIONAL
(2022)
Article
Pharmacology & Pharmacy
Meizhen Su, Ruizhe Tang, Hao Wang, Liqun Lu
Summary: Berberine hydrochloride (BBH) effectively inhibits the replication of Cyprinid herpesvirus 2 (CyHV-2) and provides protection for Crucian carps, showing favorable pharmacokinetic properties in vivo.
ANTIVIRAL RESEARCH
(2021)
Article
Green & Sustainable Science & Technology
Yan Wang, YongZe Zhou, ChengLin Duan, Liang Wang, An Jia
Summary: The present study investigates the effects of leading edge delamination on the aerodynamics and performance of a wind turbine. The results show that increased delamination levels result in the appearance of separation bubbles and vortex systems. The aerodynamic performance of the turbine decreases with increasing delamination depth.
JOURNAL OF RENEWABLE AND SUSTAINABLE ENERGY
(2022)
Article
Fisheries
Mingyang Xue, Nan Jiang, Yuding Fan, Tao Yang, Mao Li, Wenzhi Liu, Yiqun Li, Bo Li, Lingbing Zeng, Yong Zhou
Summary: This study investigated the effects of white spot syndrome virus (WSSV) infection on intestinal morphology and gut microbiota in crayfish. The results showed that WSSV infection caused damage to the intestine and hepatopancreas, decreased diversity and richness of the gut microbiota, and altered the composition and function of the microbiota.
AQUACULTURE REPORTS
(2022)
Article
Fisheries
Zidong Xiao, Mingyang Xue, Xingbing Wu, Lingbing Zeng, Yongjiu Zhu, Nan Jiang, Yuding Fan, Yong Zhou
Summary: This study is the first to isolate Staphylococcus warneri from cultured Coreius guichenoti and describe its antibiotic resistance and the histopathological changes it causes.
AQUACULTURE REPORTS
(2022)
Article
Fisheries
Jingjing Zhang, Yiqun Li, Yong Zhou, Nan Jiang, Yuding Fan, Ge Lin, Lingbing Zeng
Summary: This study identified and investigated the OAS gene (AdOAS) in Chinese Giant Salamander, showing that AdOAS plays a crucial role against GSIV infection.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Fisheries
Chen Xu, Jie Yang, JiaJia Cao, Nan Jiang, Yong Zhou, Lingbing Zeng, Qiwang Zhong, Yuding Fan
Summary: This study investigated the protein levels in the spleen of rare minnow after infection with different genotypes of GCRV II using iTRAQ-based quantitative proteomics. The results showed that both virulent and attenuated isolates induced similar upregulated proteins related to ATP synthesis, while the virulent genotype induced more immunoproteins and increased levels of ubiquitin-proteasome system members. The DEPs in the attenuated infection group changed more quickly, indicating their sensitivity during virus infection.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Zidong Xiao, Xudong Li, Mingyang Xue, Mengwei Zhang, Wei Liu, Yuding Fan, Xihua Chen, Zhipeng Chu, Feilong Gong, Lingbing Zeng, Yong Zhou
Summary: This study confirms that Vibrio metschnikovii is the pathogenic bacterium causing disease in hybrid sturgeon and reveals its potential risks to the sturgeon farming industry. The results provide a basis for the diagnosis and prevention of this disease.
Article
Fisheries
Yanlin Dai, Yiqun Li, Ge Lin, Jingjing Zhang, Nan Jiang, Wenzhi Liu, Yan Meng, Yong Zhou, Yuding Fan
Summary: This study investigated the cell death mechanisms involved in grass carp reovirus - HH196 (GCRV-HH196) infection using a grass carp ovary cell line (GCO cells). Results showed that GCRV-HH196 infection caused DNA damage, cell volume reduction, and cytoplasm shrinkage. The expression levels of pro-apoptotic genes were up-regulated and caspase proteins involved in apoptosis were activated. Flow cytometry analysis revealed a higher number of apoptotic cells in GCRV-HH196-infected cells compared to control cells. Additionally, autophagy was also involved in the regulation of GCRV-HH196 infection.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jingjing Zhang, Yanlin Dai, Yuding Fan, Nan Jiang, Yong Zhou, Lingbing Zeng, Yiqun Li
Summary: In this study, the GPI-MT-I gene in the Chinese giant salamander was characterized. The overexpression of AdGPI-MT-I protein was found to significantly reduce the replication of GSIV in salamander cells, providing insights into the antiviral mechanism against iridovirus infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yuchen Liu, Nan Jiang, Wenzhi Liu, Yong Zhou, Mingyang Xue, Qiwang Zhong, Zhong Li, Yuding Fan
Summary: In this study, we cloned and sequenced the rag1 and rag2 genes of rice-field eel and investigated their expressions in different tissues. The results showed that multiple tissues of rice-field eel contain lymphocyte lineage cells and undergo lymphopoiesis, with the thymus being the primary lymphopoietic tissue and the liver and kidney also involved in lymphocyte differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemical Research Methods
Chen Xu, Yiqun Li, Zidong Xiao, Jie Yang, Mingyang Xue, Nan Jiang, Yan Meng, Wenzhi Liu, Yuding Fan, Yong Zhou
Summary: Cyprinid herpesvirus 2 (CyHV-2) is a double-stranded DNA virus that causes severe herpesviral hematopoietic necrosis disease (HVHND) in gibel carp. This study used proteomic and phosphoproteomic analysis methods to uncover the host response to CyHV-2 infection in the kidneys of symptomatic and diseased gibel carp. The results showed that proteins involved in energy generation and ion balance were downregulated in the host after CyHV-2 infection, and phosphorylated proteins mainly participated in the regulation of RNA processing, translation, cytoskeleton organization, and immunoreaction. Furthermore, potential host kinases that interacted with viral proteins were identified, which could be candidate targets for disease treatment in the future.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Immunology
Zhao-Ran Dong, Qing-Jiang Mu, Wei-Guang Kong, Da-Cheng Qin, Yong Zhou, Xin-You Wang, Gao-Feng Cheng, Yang-Zhi Luo, Tao-Shan Ai, Zhen Xu
Summary: This study developed an oral vaccine against CyHV-2 using yeast cell surface display technology and demonstrated its protective efficacy in gibel carp. The vaccine group showed higher activities of immune-related enzymes and gene expression, and strong innate and adaptive immune responses were elicited in mucosal and systemic tissues after oral vaccination with the yeast vaccine.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Fisheries
Nan Jiang, Jinyu Shen, Yong Zhou, Wenzhi Liu, Yan Meng, Yiqun Li, Mingyang Xue, Chen Xu, Yuding Fan
Summary: In this study, a droplet digital PCR method was established to detect and quantify the genome copy number of largemouth bass ranavirus (LMBRaV), providing a specific and sensitive tool for rapid diagnosis of LMBRaV infection in largemouth bass and quantification of the virus load.
JOURNAL OF FISH DISEASES
(2023)
Article
Agriculture, Dairy & Animal Science
Yan Meng, Yuding Fan, Nan Jiang, Mingyang Xue, Yiqun Li, Wenzhi Liu, Lingbing Zeng, Yong Zhou
Summary: This study investigated the Mx genes in Andrias davidianus and found that they share structural features and functions with Mx genes in other species. The expression of these genes was different in healthy and infected individuals. Understanding the antiviral immunity of A. davidianus is important for captive breeding and wildlife protection.
Article
Fisheries
Yangyang Xing, Mingyang Xue, Zidong Xiao, Xiaowei Hu, Jiale Zhai, Chunjie Zhang, Nan Jiang, Yuding Fan, Yan Meng, Yong Zhou
Summary: In this study, the pathogen causing the death of farmed Pelodiscus sinensis was isolated and identified as Chryseobacterium indologenes. Histopathological analysis revealed the pathological changes in tissues, and antibiotic susceptibility tests showed that the bacteria could be treated with certain antibiotics. These findings contribute to the future diagnosis and treatment of the disease.
JOURNAL OF FISH DISEASES
(2023)
Article
Agriculture, Dairy & Animal Science
Naicheng Liu, Peng Zhang, Mingyang Xue, Zidong Xiao, Mengjie Zhang, Yan Meng, Yuding Fan, Junqiang Qiu, Qinghua Zhang, Yong Zhou
Summary: In this study, the bacterial community structure of Chinese soft-shelled turtles (SST) and its aquaculture water system were analyzed using Illumina MiSeq sequencing. The results showed that the bacterial diversity and richness compositions in the intestinal tract and aquaculture water were the same, but the relative abundances of bacterial communities varied. The intestinal microorganisms in the SSTs experienced significant changes after transfer to a different environment.