期刊
VETERINARY MICROBIOLOGY
卷 163, 期 1-2, 页码 62-70出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetmic.2012.12.017
关键词
Foot-and-mouth disease; Replicase-based DNA vaccine; Multivalent epitope vaccine; Prime-boost vaccination regime; Immune response; Cattle
资金
- Department of Biotechnology (DBT), Government of India
- IVRI Research fellowship
The limited efficacy of DNA vaccines against foot-and-mouth disease (FMD) in cattle and other natural hosts has prompted a search for a more effective vaccination regimen. In this study we tested a DNA prime-protein boost vaccination strategy against FMD in bovine calves. We used purified recombinant FMDV specific multi-epitope protein (rMEG990) and an optimized sindbis virus replicase-based DNA vaccine expressing this protein (pSinCMV-Vac-MEG990). We demonstrate that vaccination with a low dose of pSinCMV-Vac-MEG990 (10 mu g/animal) and subsequently boosting with rMEG990 resulted in induction of neutralizing antibodies, IFN-gamma production and protection against homologous virus challenge. However, vaccination with a high dose of pSinCMV-Vac-MEG990 (100 mu g/animal) and boosting with rMEG990 resulted in significantly lower immune responses and more severity to the challenge test. Additionally, we show that the post-vaccinal IFN-gamma levels in animals correlated positively to their protection against FMDV challenge. These findings suggest that a replicase-based DNA vaccine in proper prime-boost combination may offer an efficient vaccine strategy against FMDV and that IFN-gamma could be used as an additional immune parameter to predict protection against FMDV infection. (c) 2012 Elsevier B.V. All rights reserved.
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