期刊
VETERINARY MICROBIOLOGY
卷 155, 期 2-4, 页码 220-229出版社
ELSEVIER
DOI: 10.1016/j.vetmic.2011.09.018
关键词
PRRSV; NSP1; Monoclonal antibody; Epitopes
资金
- National Key Genomic Transformation Program [2009ZX08009-143B]
- National Natural Science Foundation [30871868]
- special fund for Agro-Scientific Research in the Public Interest [200803020-5, 201003060-04]
Porcine reproductive and respiratory syndrome virus (PRRSV) was divided into North American and European genotypes. NSP1 was an important non-structural protein of PRRSV, which was auto-cleaved from the replicase polyprotein into NSP1 alpha and NSP1 beta subunits and played an important role in the immune suppression. In this study, six monoclonal antibodies (MAbs) against the recombinant PRRSV NSP1, expressed in Escherichia coli system, were screened out and identified. Western blot and IFA results indicated that 4 out of 6 MAbs recognized the recombinant NSP1 alpha and 2 MAbs recognized NSP1 beta. Epitope mapping results indicated that MAb 4H2 recognized the linear epitopes E(54)EPLRW(59) in NSP1 alpha, MAbs (2G5, 3E11 and 4D4) recognized the epitopes H(157)VLTNLP(163) in NSP1 alpha, and MAbs 3C7 and 1H7 reacted with the epitopes 185aa to 232aa in NSP1 beta. Protein sequence alignment of NSP1 indicated E(54)EPLRW(59) was conserved in all North American PRRSV strains, whereas European type strains has variable amino acids in this region. The epitope H(157)VLTNLP(163) was relatively conserved among all PRRSV strains, except for a L162 -> S162 change in European type strains. The epitope 185-232aa was variable among North American PRRSV strains. These results may facilitate future investigations into the function of NSP1 of PRRSV and diagnostic methods for PRRSV infection. (C) 2011 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据