期刊
VETERINARY MICROBIOLOGY
卷 156, 期 1-2, 页码 200-204出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetmic.2011.10.012
关键词
Classical swine fever virus; Dendrimeric peptide; Neutralizing epitope; T helper cell epitope; Marker vaccine
资金
- Shanghai Science and Technology Program for Agriculture [2009-5-4]
The amino acid sequence (TAVSPTTLR, 829-837 aa) on the glycoprotein E2 of classical swine fever virus (CSFV) is a conserved and linear neutralizing epitope. In the present study, two peptides were constructed based the core sequence of this neutralizing epitope, the dendrimeric peptide (Th-B-4) containing four copies of B cell epitope fused to one copy of promiscuous T helper (Th) cell epitope and the peptide Th-B containing a single copy of B cell epitope fused to one copy of Th cell epitope. The dendrimeric peptide Th-B-4 elicited high titers of neutralizing antibodies as detected in an indirect ELISA, blocking ELISA and neutralization test and induced a complete protection against CSFV C strain in rabbits. The Th-B elicited low titers of neutralizing antibodies and did not induce a protection in rabbits. These results suggest that the dendrimeric peptide Th-B-4 may be a promising marker vaccine candidate against CSFV and the multimerization is a requirement for development of a peptide vaccine. (C) 2011 Elsevier B.V. All rights reserved.
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