Article
Biotechnology & Applied Microbiology
V. L. Ely, D. I. B. Pereira, M. M. da Costa, L. Panagio, G. Nakasato, G. Reis, J. F. Cargnelutti, L. A. Sangioni, S. A. Botton
Summary: This study evaluated the therapeutic potential of biogenic silver nanoparticles (Bio-AgNP) against Prototheca spp. The results showed that Bio-AgNP exhibited algaecide activity against Prototheca spp., indicating its potential as a novel therapeutic approach.
LETTERS IN APPLIED MICROBIOLOGY
(2022)
Review
Biology
Giancarlo Bozzo, Michela Maria Dimuccio, Gaia Casalino, Edmondo Ceci, Marialaura Corrente
Summary: This article discusses the issue of protothecosis, a potential zoonosis related to bovine mastitis, and proposes new approaches to farm management based on risk analysis to control its spread among farms. According to EU regulations, diseases caused by Prototheca species are listed as transmissible animal diseases, which can have significant implications for human and animal health, as well as feed and food safety.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Tomasz Jagielski, Mateusz Iskra, Joanna Rudna, Katarzyna Roeske, Julita Nowakowska, Jacek Bielecki, Henryk Krukowski
Summary: The study investigates the occurrence of very rare and poorly studied microalgae of the genus Prototheca, potentially pathogenic to humans and animals, in different water environments. Given the potential hazard to human and animal health from exposure to water-inhabiting protothecae, the prevalence of the algae in aquatic habitats deserves an insightful examination.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Immunology
Jian Guo, Juan Chen, Teng Li, Lei Gao, Cizhong Jiang, Wenjuan Wu
Summary: In this study, a mucous-like strain of P. wickerhamii with atypical colony morphology was identified. The transcriptomics, proteomics, and metabolomics of this strain and two other P. wickerhamii strains were analyzed to reveal the morphological differences and molecular basis of pathogenicity. Mannan endo-1,4-beta-mannosidase was found to be significantly downregulated in the mucous-like strain, resulting in a thinner cell wall and reduced toxicity to macrophages. Metabolite analysis suggested that the mucoid appearance may be caused by increased levels of linoleic acid, glycerol, and other metabolites. Further study is needed to understand the ecology, aetiology, and pathogenesis of P. wickerhamii, particularly its transmission between humans, animals, and the environment, from a One Health perspective.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Immunology
Wenpeng Zhao, Maolin Xu, Herman W. W. Barkema, Xiaochen Xie, Yushan Lin, Sohrab Khan, John P. P. Kastelic, Dong Wang, Zhaoju Deng, Bo Han
Summary: Prototheca bovis induces autophagy in bovine mammary epithelial cells (bMECs) through the HIF-1 alpha and AMPK alpha/ULK1 pathway, involving lysosome-associated proteins Rab7 and LAMP2a.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Takahito Toyotome, Shihori Matsui
Summary: This study surveyed the yeast species and Prototheca species isolated from bulk tank milk in the Tokachi district of Japan from April 2020 through March 2021. The most prevalent yeast species was Pichia kudriavzevii, while Prototheca bovis was the only Prototheca species isolated. The study also observed seasonal variations in the presence of certain yeast species.
JOURNAL OF DAIRY SCIENCE
(2022)
Review
Microbiology
Balazs Libisch, Carine Picot, Andres Ceballos-Garzon, Monika Moravkova, Marcela Klimesova, Gabor Telkes, Shih-Te Chuang, Patrice Le Pape
Summary: Prototheca microalgae, recognized as pathogens in both humans and animals, have gained increasing interest in human and veterinary medicine. They have become an important cause of bovine mastitis and pose challenges to dairy farm management. Effective treatment is currently lacking, highlighting the importance of prevention.
Article
Infectious Diseases
Valentina Monistero, Antonio Barberio, Paola Cremonesi, Bianca Castiglioni, Stefano Morandi, Desiree C. K. Lassen, Laerke B. Astrup, Clara Locatelli, Renata Piccinini, M. Filippa Addis, Valerio Bronzo, Paolo Moroni
Summary: The study revealed that Strep. uberis is an environmental pathogen responsible for mastitis in dairy cows in Italy, showing resistance to multiple antimicrobials with a low prevalence of beta-lactam resistance. The genotypic variability of the isolates was investigated using multiplex PCR and RAPD-PCR typing, along with phenotypic antimicrobial resistance assessment. The highest resistance rate was observed for lincomycin, with most isolates showing resistance to at least one drug tested.
Review
Dermatology
Feifan Chen, Mario Saab-Chalhoub, Joy Tao, Amanda T. Harrington, Fritzie S. Albarillo, Andrew S. Crone, Nina M. Clark, Jodi J. Speiser
Summary: Prototheca species are rare algae that can cause infection in humans. It typically manifests as localized cutaneous disease and can rarely become disseminated. Immunocompromised patients are at highest risk for disseminated protothecosis, which has a higher mortality rate than localized infections. Prototheca zopfii infections are reported less frequently than Prototheca wickerhamii infections. Diagnosis can be made using histopathology, culture, and molecular testing. Treatment options are not definitive, but antifungal drugs like amphotericin B are commonly used. We present an additional case of disseminated protothecosis caused by P. zopfii in a post-bone marrow transplant patient in the Midwest of the United States.
AMERICAN JOURNAL OF DERMATOPATHOLOGY
(2023)
Article
Immunology
Jian Guo, Jianbo Jian, Lili Wang, Lijuan Xiong, Huiping Lin, Ziyi Zhou, Eva C. Sonnenschein, Wenjuan Wu
Summary: This study sequenced the genomes of two strains of P. wickerhamii to gain insight into the genetic basis for its pathogenicity and provide a basis for future prevention and treatment strategies. The analysis revealed the evolutionary relationship of Prototheca and identified enriched genes in metabolic pathways.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
S. Pegolo, R. Tessari, V Bisutti, A. Vanzin, D. Giannuzzi, M. Gianesella, A. Lisuzzo, E. Fiore, A. Barberio, E. Schiavon, E. Trevisi, F. Piccioli Cappelli, L. Gallo, P. Ruegg, R. Negrini, A. Cecchinato
Summary: This study investigated the associations between subclinical intra-mammary infection (IMI) and milk composition, udder health indicators, and cheesemaking traits. The results showed that udder health indicators were associated with increased SCC and IMI, and IMI had detrimental effects on milk quality and cheesemaking ability.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Mariola Bochniarz, Tomasz Piech, Tomasz Kocki, Mateusz Iskra, Henryk Krukowski, Tomasz Jagielski
Summary: The study confirmed that the decrease in tryptophan and its metabolites in milk samples, along with the increase in activity of indoleamine 2,3-dioxygenase, could be used as markers of mastitis due to Prototheca algae.
Article
Veterinary Sciences
Maria P. Huilca-Ibarra, David Vasco-Julio, Yanua Ledesma, Salome Guerrero-Freire, Jeannete Zurita, Pablo Castillejo, Francisco Barcelo Blasco, Lisseth Yanez, Darwin Changoluisa, Gustavo Echeverria, Carlos Bastidas-Caldes, Jacobus H. de Waard
Summary: This study confirms the presence of Prototheca bovis in cows with mastitis in Ecuador and evaluates its infection rate. The detection and disinfection methods for P.bovis are discussed, and recommendations to increase diagnostic yield are proposed.
VETERINARY SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Wenpeng Zhao, Zhaoju Deng, Herman W. Barkema, Maolin Xu, Jian Gao, Gang Liu, Yushan Lin, John P. Kastelic, Bo Han
Summary: Prototheca bovis infection induces inflammatory responses in mouse mammary gland tissue and mammary epithelial cells. The NF-kappa B/NLRP3 inflammasome pathway plays a crucial role in the inflammatory response, while scavenging ROS or activating Nrf2 can mitigate this response.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Correction
Multidisciplinary Sciences
Marco Severgnini, Barbara Lazzari, Emanuele Capra, Stefania Chessa, Mario Luini, Roberta Bordoni, Bianca Castiglioni, Matteo Ricchi, Paola Cremonesi
Summary: An amendment to this paper has been published and a link to access it is available at the top of the paper.
SCIENTIFIC REPORTS
(2022)
Article
Microbiology
Narda Medina, Juan Carlos Soto-Debran, Danila Seidel, Isin Akyar, Hamid Badali, Aleksandra Barac, Stephane Bretagne, Yasemin Cag, Carole Cassagne, Carmen Castro, Arunaloke Chakrabarti, Eric Dannaoui, Celia Cardozo, Julio Garcia-Rodriguez, Juliette Guitard, Petr Hamal, Martin Hoenigl, Tomasz Jagielski, Sadegh Khodavaisy, Giuliana Lo Cascio, Maria Carmen Martinez-Rubio, Joseph Meletiadis, Patricia Munoz, Elzbieta Ochman, Teresa Pelaez, Ana Perez-Ayala Balzola, Juergen Prattes, Emmanuel Roilides, Maite Ruiz-Perez de Pipaon, Raphael Stauf, Joerg Steinmann, Ana Isabel Suarez-Barrenechea, Rocio Tejero, Laura Trovato, Lourdes Vinuela, Thanwa Wongsuk, Iwona Zak, Hossein Zarrinfar, Cornelia Lass-Florl, Sevtap Arikan-Akdagli, Ana Alastruey-Izquierdo
Summary: Invasive candidiasis caused by mixed yeast (MY) infections was studied in a multicenter prospective research, with Europe having the highest MY rate. The most frequent combinations were Candida albicans/C. glabrata and C. albicans/C. parapsilosis. All Candida isolates were susceptible to amphotericin B.
Article
Biotechnology & Applied Microbiology
Zofia Bakula, Pawel Siedlecki, Robert Gromadka, Jan Gawor, Agnieszka Gromadka, Jan J. Pomorski, Hanna Panagiotopoulou, Tomasz Jagielski
Summary: This study is the first to describe the genetic make-up of P. wickerhamii and discover proteins possibly involved in the development of protothecosis.
Article
Veterinary Sciences
Carlos E. Fidelis, Manoela Franke, Leticia C. R. de Abreu, Tomasz Jagielski, Marcio G. Ribeiro, Marcos dos Santos, Juliano L. Goncalves
Summary: The study evaluated the use of MALDI-TOF MS for the rapid and accurate identification of three major Prototheca species in mastitis cases. With an extended algae database, MALDI-TOF MS demonstrated the ability to quickly and effectively identify Prototheca species from mastitis cases.
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
(2021)
Article
Multidisciplinary Sciences
Alina Minias, Filip Gasior, Anna Brzostek, Tomasz Jagielski, Jaroslaw Dziadek
Summary: The study found that vitamin B12 synthesis occurs in mycobacteria, with the probable exception of M. tuberculosis. This provides direct evidence of vitamin B12 synthesis in a clinically important group of bacteria.
SCIENTIFIC REPORTS
(2021)
Editorial Material
Respiratory System
Sophia B. Georghiou, Timothy C. Rodwell, Alexei Korobitsyn, Said H. Abbadi, Kanchan Ajbani, Jan-Willem Alffenaar, David Alland, Nataly Alvarez, Sonke Andres, Elisa Ardizzoni, Alexandra Aubry, Rossella Baldan, Marie Ballif, Ivan Barilar, Erik C. Bottger, Soumitesh Chakravorty, Pauline M. Claxton, Daniela M. Cirillo, Inaki Comas, Chris Coulter, Claudia M. Denkinger, Brigitta Derendinger, Edward P. Desmond, Jurriaan E. M. de Steenwinkel, Keertan Dheda, Andreas H. Diacon, David L. Dolinger, Kelly E. Dooley, Matthias Egger, Maha R. Farhat, Lanfranco Fattorini, Iris Finci, Laure Fournier Le Ray, Victoria Furio, Ramona Groenheit, Tawanda Gumbo, Scott K. Heysell, Doris Hillemann, Harald Hoffmann, Po-Ren Hsueh, Yi Hu, Hairong Huang, Alamdar Hussain, Farzana Ismail, Kiyohiko Izumi, Tomasz Jagielski, John L. Johnson, Priti Kambli, Kone Kaniga, G. H. R. Eranga Karunaratne, Meenu Kaushal Sharma, Peter M. Keller, Ellis C. Kelly, Margarita Kholina, Mikashmi Kohli, Katharina Kranzer, Ian F. Laurenson, Jason Limberis, S-Y Grace Lin, Yongge Liu, Alexandre Lopez-Gavin, Anna Lyander, Diana Machado, Elena Martinez, Faisal Masood, Satoshi Mitarai, Nomonde R. Mvelase, Stefan Niemann, Vladyslav Nikolayevskyy, Florian P. Maurer, Matthias Merker, Paolo Miotto, Shaheed Omar, Ralf Otto-Knapp, Moises Palaci, Juan Jose Palacios Gutierrez, Sharon J. Peacock, Charles A. Peloquin, Jennifer Perera, Catherine Pierre-Audigier, Suporn Pholwat, James E. Posey, Therdsak Prammananan, Leen Rigouts, Jaime Robledo, Neesha Rockwood, Camilla Rodrigues, Max Salfinger, Marcos C. Schechter, Marva Seifert, Sarah Sengstake, Thomas Shinnick, Natalia Shubladze, Vitali Sintchenko, Frederick Sirgel, Sulochana Somasundaram, Timothy R. Sterling, Andrea Spitaleri, Elizabeth Streicher, Philip Supply, Erik Svensson, Elisa Tagliani, Sabira Tahseen, Akiko Takaki, Grant Theron, Gabriela Torrea, Armand Van Deun, Annelies Van Rie, Dick van Soolingen, Roger Vargas, Amour Venter, Nicolas Veziris, Cristina Villellas, Miguel Viveiros, Robin Warren, Shu'an Wen, Jim Werngren, Robert J. Wilkinson, Caie Yang, Tingting Zhang, Danila Zimenkov, Nazir Ismail, Thomas Schon, Claudio U. Koser, Soudeh Ehsani, Jakko van Ingen, F. Ferda Yilmaz
EUROPEAN RESPIRATORY JOURNAL
(2022)
Article
Multidisciplinary Sciences
Zofia Bakula, Valentine B. I. Wuyep, Lukasz Bartocha, Anna Vyazovaya, Eugene I. Ikeh, Jacek Bielecki, Igor Mokrousov, Tomasz Jagielski
Summary: Nigeria has the highest burden of tuberculosis in Africa and ranks 6th globally. However, few studies have examined the molecular epidemiology of Mycobacterium tuberculosis in the country. This study analyzed the genetic structure of drug-resistant tuberculosis in central Nigeria and found that the Cameroon and T lineages were the most prevalent, with no association between the Cameroon lineage and drug resistance.
Article
Biotechnology & Applied Microbiology
Tomasz Jagielski, Mateusz Iskra, Joanna Rudna, Katarzyna Roeske, Julita Nowakowska, Jacek Bielecki, Henryk Krukowski
Summary: The study investigates the occurrence of very rare and poorly studied microalgae of the genus Prototheca, potentially pathogenic to humans and animals, in different water environments. Given the potential hazard to human and animal health from exposure to water-inhabiting protothecae, the prevalence of the algae in aquatic habitats deserves an insightful examination.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Editorial Material
Infectious Diseases
Tomasz Jagielski
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Article
Immunology
Zofia Bakula, Mateusz Marczak, Agata Bluszcz, Malgorzata Proboszcz, Justyna Kosciuch, Rafal Krenke, Petras Stakenas, Igor Mokrousov, Tomasz Jagielski
Summary: This study investigated the genetic diversity of Mycobacterium tuberculosis isolates in Poland, with a focus on multidrug-resistant strains. The results showed that the Beijing genotype was the most prevalent among MDR-TB cases, especially among foreign-born patients, indicating possible transmission from FSU countries. These findings are important for monitoring and controlling the spread of tuberculosis in Poland.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Dermatology
Wendie Roldan Villalobos, Tassia Ferreira, Vanessa Gmyterco, Renata Bastos, Louise Bacher, Tomasz Jagielski, Marcio Ribeiro, Marconi Farias
Summary: A domestic cat was diagnosed with Prototheca wickerhamii infection through cytological and histopathological examination, and culture. Intralesional application of amphotericin B in conjunction with oral terbinafine resulted in a significant reduction of the nasal lesion and complete resolution of the pinnal lesion, without adverse effects.
VETERINARY DERMATOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Marcelo Fagali Arabe Filho, Tomasz Jagielski, Angelika Proskurnicka, Marcos Veiga dos Santos, Carlos Eduardo Fidelis, Felipe Freitas Guimaraes, Simony Trevizan Guerra, Samea Fernandes Joaquim, Mateus de Souza Ribeiro Mioni, Jose Carlos de Figueiredo Pantoja, Helio Langoni, Luisa Fernanda Garcia Sanchez, Marcio Garcia Ribeiro
Summary: The in vitro algaecide activity of quaternary ammonium (QA) against Prototheca isolated from bovine clinical mastitis was investigated. The study highlights the predominance of P. bovis as the causative agent of algal mastitis in bovines, and the strong algaecide activity of QA at low concentrations against Prototheca.
Article
Microbiology
David Vasco-Julio, Maria Huilca-Ibarra, Yanua Ledesma, Gustavo Echeverria, Salome Guerrero-Freire, Tomasz Jagielski, Carlos Bastidas-Caldes, Jacobus H. de Waard
Summary: A multiplex PCR system (m-PCR) has been developed for accurate differentiation of five important pathogenic Prototheca species. The method is cost-effective and uses a simple heat-shock method in a TE buffer for DNA extraction. The method can identify the species within three hours and can detect mixed infections involving two or three Prototheca species.
Article
Veterinary Sciences
Vanessa Cunningham Gmyterco, Tomasz Jagielski, Gustavo Baldasso, Louise Helene Bacher, Marcio Garcia Ribeiro, Marconi Rodrigues de Farias
Summary: This article reports a case of chronic cutaneous protothecosis in a dog in Brazil, successfully treated with long-term pulse therapy with itraconazole. The study suggests that oral itraconazole pulse dosing can effectively control this skin infection.
ACTA VETERINARIA SCANDINAVICA
(2023)
Article
Mathematical & Computational Biology
Mikolaj Dziurzynski, Przemyslaw Decewicz, Mateusz Iskra, Zofia Bakula, Tomasz Jagielski
Summary: Prototheca is a genus of unicellular, achlorophyllous, yeast-like algae widely distributed in the environment, known to infect vertebrates including humans. Protothecosis can be clinically challenging and treatment-resistant, requiring accurate identification through DNA sequencing. Current limitations in sequence databases make Prototheca identification difficult and inconclusive, with a lack of bioinformatic tools for data processing and analysis. The introduction of Prototheca-ID aims to address these challenges by providing a user-friendly, web-based application for fast and reliable speciation of Prototheca isolates.
DATABASE-THE JOURNAL OF BIOLOGICAL DATABASES AND CURATION
(2021)
Review
Mycology
Michiaki Masuda, Tomasz Jagielski, Patrizia Danesi, Christian Falcaro, Michela Bertola, Mark Krockenberger, Richard Malik, Rui Kano
Summary: Protothecosis is a disease of humans and animals caused by infection with fungus-like, colourless microalgae of the genus Prototheca. While still uncommon, there is an increasing number of diagnosed cases worldwide. This review summarizes new findings in basic science and clinical features, diagnosis and treatment of protothecosis in dogs and cats based on information presented at a medical phycology symposium.
