期刊
VETERINARY MICROBIOLOGY
卷 147, 期 1-2, 页码 67-74出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetmic.2010.05.044
关键词
(GTG)(5)-PCR fingerprinting; Bovine; Coagulase-negative staphylococci; Mastitis
资金
- Research Council of the Vrije Universiteit Brussel
- Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT-Vlaanderen) [60714]
Due to significant financial losses in the dairy cattle farming industry caused by mastitis and the possible influence of coagulase-negative staphylococci (CNS) in the development of this disease, accurate identification methods are needed that untangle the different species of the diverse CNS group. In this study, 39 Staphylococcus type strains and 253 field isolates were subjected to (GTG)(5)-PCR fingerprinting to construct a reference framework for the classification and identification of different CNS from (sub)clinical milk samples and teat apices swabs. Validation of the reference framework was performed by dividing the field isolates in two separate groups and testing whether one group of field isolates, in combination with type strains, could be used for a correct classification and identification of a second group of field isolates. (GTG)(5)-PCR fingerprinting achieved a typeability of 94.7% and an accuracy of 94.3% compared to identifications based on gene sequencing. The study shows the usefulness of the method to determine the identity of bovine Staphylococcus species, provided an identification framework updated with field isolates is available. (C) 2010 Elsevier B.V. All rights reserved.
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