期刊
VETERINARY MICROBIOLOGY
卷 147, 期 3-4, 页码 383-388出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetmic.2010.07.011
关键词
Antimicrobial susceptibility; Bovine; Escherichia coli; Mastitis; Phylogeny; Virulence factors
资金
- Walter Ehrstrom Foundation
- Mercedes Zachariassen Foundation
- Finnish Veterinary Foundation
- Lammi-Tuulos Health Centre
- Hame Regional Foundation of the Finnish Cultural Foundation
The aim of this study was to identify specific phylogeny groups, virulence genes or antimicrobial resistance traits of Escherichia colt isolated in bovine mastitis associated to clinical signs, persistence of intramammary infection in the quarter and recovery from mastitis. A total of 154 E. colt isolates from bovine clinical mastitis, 144 from the acute stage and 10 from follow-up samples 3 weeks later, originating from 144 cows in 65 dairy herds in Southern Finland were investigated. Phylogeny groups and virulence genes of the isolates were determined using polymerase chain reaction, and antimicrobial susceptibility using the VetMIC (TM) microdilution method. In ten cows (11.8%), infection persisted, confirmed by re-isolation of the same pulsed-field gel electrophoresis type from the affected quarter at 3 weeks post-treatment. The majority of isolates, 119(82.6%). belonged to phylogeny group A, which mainly consisted of commensal strains. Altogether 56 isolates (38.9%) had at least one virulence gene detected. Most common virulence genes detected were irp2, iucD, papC iss; genes svg, stx1, stx2, cnf1 and hlyA were not found. Combinations of virulence genes varied greatly. Forty (27.8%) of the 144 E. colt isolates showed resistance to at least one antimicrobial tested. None of the studied phylogeny groups, virulence factors or antimicrobial resistance traits was associated with clinical signs, persistence of intramammary infection or clinical recovery from mastitis. The results support the conclusion that mastitis-causing E. coli bacteria are typical commensals. (C) 2010 Elsevier B.V. All rights reserved.
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