Article
Microbiology
Craig S. Ross, Sahar Mahmood, Paul Skinner, Jo Mayers, Scott M. Reid, Rowena D. E. Hansen, Ashley C. Banyard
Summary: Newcastle disease (ND) is a highly contagious disease caused by avian paramyxovirus-1. It is endemic in poultry in developing countries and can still cause outbreaks in developed countries, impacting both commercial and backyard flocks. Outbreaks of ND can have significant economic consequences and no antiviral treatments are available currently.
JOURNAL OF MEDICAL MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Jongseo Mo, Michael Angelichio, Lisa Gow, Valerie Leathers, Mark W. Jackwood
Summary: This study adapted pre-existing molecular-based diagnostic assays for NDV and ILTV, and developed new assays for aMPV A and B, which showed equivalent performance with high specificity under synchronized thermocycling conditions. Identical amplification conditions will simplify procedures for detection in diagnostic laboratories.
JOURNAL OF VETERINARY SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Dominika Bebnowska, Rafal Hrynkiewicz, Paulina Niedzwiedzka-Rystwej
Summary: Real-time PCR is a useful method for rapid, specific, and effective virus detection, especially for identifying the Lagovirus europaeus causing rabbit hemorrhagic disease. Molecular research is essential for solving epidemic issues related to pathogen spread, and real-time PCR has been shown to be a fast and specific tool for detecting RNA viruses infecting rabbits.
APPLIED SCIENCES-BASEL
(2021)
Article
Infectious Diseases
Naomi Ariyama, Rodrigo Tapia, Claudia Godoy, Belen Aguero, Valentina Valdes, Felipe Berrios, Pablo Garcia Borboroglu, Klemens Putz, Raul Alegria, Gonzalo P. Barriga, Rafael Medina, Victor Neira
Summary: The study identified antibodies against Avian orthoavulavirus 1 (AOaV-1) in various penguin species in the Antarctic and Sub-Antarctic regions, with Magellanic penguins potentially serving as a natural reservoir. The highest seropositivity rate was observed in Magellanic penguins, while Chinstrap penguins in the Antarctic regions had the highest occurrence. No seroreactivity was found in penguins in Southern locations, indicating further studies are needed to understand the epidemiology and effects of AOaV-1 on these populations.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Veterinary Sciences
Vinay G. Joshi, Deepika Chaudhary, Nitish Bansal, Renu Singh, Sushila Maan, Nand K. Mahajan, Chintu Ravishankar, Niranjana Sahoo, Sunil K. Mor, Jessica Radzio-Basu, Catherine M. Herzog, Vivek Kapur, Parveen Goel, Naresh Jindal, Sagar M. Goyal
Summary: Newcastle disease virus (NDV) causes Newcastle disease in poultry in India, with a 9.8% apparent prevalence rate found in a surveillance study. The presence of NDV in commercial and backyard poultry emphasizes the importance of surveillance studies even in apparently healthy flocks. The study contributes to a better understanding of NDV epidemiology and could help formulate disease control strategies for both commercial and backyard birds.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
Jong-Min Kim, Hye-Ryung Kim, Gyu-Tae Jeon, Ji-Su Baek, Oh-Deog Kwon, Choi-Kyu Park
Summary: A highly specific and sensitive dqRT-PCR assay was developed for simultaneous detection of PPIV1 and PPIV5 in clinical pig samples. The assay showed high sensitivity, specificity, and accuracy, and was able to detect PPIV1 and PPIV5 in Korean pig herds. Phylogenetic analysis revealed genetically diverse PPIV1 strains in Korean pig herds. This dqRT-PCR assay will be a valuable tool for diagnosis and further studies on PPIV1 and PPIV5.
Article
Biochemical Research Methods
Andrea Fortin, Andrea Laconi, Isabella Monne, Siamak Zohari, Kristofer Andersson, Christian Grund, Mattia Cecchinato, Marika Crimaudo, Viviana Valastro, Valeria D'Amico, Alessio Bortolami, Michele Gastaldelli, Maria Varotto, Calogero Terregino, Valentina Panzarin
Summary: This study presents a new real-time RT-PCR method that can rapidly differentiate between virulent and avirulent viruses. The method has been validated and shows favorable results in terms of sensitivity, specificity, repeatability, and stability, making it suitable for routine clinical sample testing.
JOURNAL OF VIROLOGICAL METHODS
(2023)
Article
Agriculture, Dairy & Animal Science
Mengqing Yang, Jiangang Ma, Zhili Chu, Xuhong Cao, Kejia Lu, Xiaolei Shi, Lina Tong, Chuanqi Yan, Haijin Liu, Xinglong Wang, Sa Xiao, Zengqi Yang
Summary: The study revealed a potential interaction between MSI1 and NDV V protein, indicating that MSI1 may act as a new antiviral molecule by inhibiting viral release and blocking apoptosis to inhibit the release of NDV.
Article
Zoology
Ting Xu, Rongxiang Tan, Yutao Zhu, Jian Ye
Summary: This study developed a new real-time PCR method for the detection of decapod iridescent virus 1 (DIV1) DNA. The method showed good linearity, repeatability, and specificity, and efficiently, sensitively, and reliably detected DIV1.
JOURNAL OF INVERTEBRATE PATHOLOGY
(2023)
Article
Virology
Mattia Calzolari, Simone Russo, Katia Marzani, Gastone Dalmonte, Matteo Ricchi, Paolo Bonilauri
Summary: A specific real-time PCR method for Fermo virus has been developed and reported in this study. This method is of great importance for studying the epidemiology of Fermo virus and its potential pathogenicity in humans or animals.
Article
Veterinary Sciences
Shaopeng Wu, Tian Ding, Hongxia Shao, Kun Qian, Jianqiang Ye, Aijian Qin
Summary: A new quadruplex real-time PCR assay using TaqMan probes was developed to differentiate and quantify HVT, CVI988, and virulent MDV-1. The assay showed high sensitivity and specificity, with a limit of detection of 10 copies and no cross-reactivity with other avian disease viruses. Analysis of virus replication kinetics demonstrated that vaccination with CVI988 significantly reduced the viral load of MD5. This assay, combined with meq gene PCR, effectively identifies virulent MDV infections in immunized chickens.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Review
Veterinary Sciences
Shimaa M. G. Mansour, Reham M. ElBakrey, Fakry F. Mohamed, Esraa E. Hamouda, Mona S. Abdallah, Ahmed R. Elbestawy, Mahmoud M. Ismail, Hanan M. F. Abdien, Amal A. M. Eid
Summary: Avian orthoavulavirus 1 has a broad range of hosts and can cause devastating economic impact globally. The emergence of new virulent genotypes in Egypt and the concurrent evolution of distinct genotypes in different geographic locations illustrate the genetic diversity of the virus. Different APMV-1 genotypes circulate among various bird species in Egypt.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Review
Infectious Diseases
Donia Bouzid, Jordi Vila, Glen Hansen, Davide Manissero, Josep Pareja, Sonia N. Rao, Benoit Visseaux
Summary: This systematic review examines the association between respiratory pathogen rt-PCR Ct values and clinical presentation or outcomes. The findings suggest that low Ct values may be associated with disease severity and outcomes for certain pathogens.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Miaomiao Li, Yan Lv, Dawei Cui, Yushan Xu, Mengjiao Lin, Xiaojun Zhang, Yongjun Wang, Cuifen Shen, Jue Xie
Summary: This study developed a one-step pentaplex real-time reverse transcription PCR (qRT-PCR) assay for simultaneous and sensitive detection of hepatitis B virus (HBV), hepatitis C virus (HCV), hepatitis E virus (HEV), Treponema pallidum (T. pallidum), and RNase P in blood. The assay demonstrated high clinical sensitivity, specificity, and consistency, and can detect pathogens during the window period of infection, making it a valuable tool for screening blood donors and early clinical diagnosis.
BMC INFECTIOUS DISEASES
(2023)
Article
Microbiology
Xue-Lian Luo, Xiu-Dan Zhang, Bei-Jie Li, Tian Qin, Zhi-Jie Cao, Qian-Jin Fan, Jing Yang, Dong Jin, Shan Lu, Ya-Yun Zheng, Xue-Fang Xu, Ji Pu, Jianguo Xu
Summary: Lassa virus is an important human pathogen causing hemorrhagic fever in West Africa, with an increasing risk of imported cases to other countries due to increased traveling. The high nucleotide diversity of the virus strains complicates the development of diagnostic assays. The GPC RT-PCR/2007 assay and the Mabsky kit showed good sensitivity and specificity for detecting most LASV strains.
MICROBIOLOGY SPECTRUM
(2023)
Article
Astronomy & Astrophysics
Amanda G. Bendia, Flavia Callefo, Maicon N. Araujo, Evelyn Sanchez, Veronica C. Teixeira, Alessandra Vasconcelos, Gislaine Battilani, Vivian H. Pellizari, Fabio Rodrigues, Douglas Galante
Summary: The study explores microbial communities in a silica and iron-rich cave in Brazil, revealing metabolic potentials and genetic diversity of prokaryotic lineages. Through genomic analysis, novel microbial lineages related to anaerobic methane oxidation, iron oxidation, and radiation resistance were identified, providing important genomic information about these organisms with potential implications for astrobiology research.
Review
Oceanography
Juliana Correa Neiva Ferreira, Natascha M. Bergo, Pedro M. Tura, Mateus Gustavo Chuqui, Frederico P. Brandini, Luigi Jovane, Vivian H. Pellizari
Summary: Marine microbes play a crucial role in controlling the distribution and diversity of matter and energy in the oceans. This study provides the first insights into the abundance, taxonomy, and diversity of picoplankton and their ecological processes in the Fe-Mn deposit region of the Rio Grande Rise in the Southwestern Atlantic Ocean.
PROGRESS IN OCEANOGRAPHY
(2022)
Article
Environmental Sciences
Natascha Menezes Bergo, Adriana Torres-Ballesteros, Camila Negrao Signori, Mariana Benites, Luigi Jovane, Bramley J. Murton, Ulisses Nunes da Rocha, Vivian Helena Pellizari
Summary: This study analyzed the composition and diversity of bacteria and archaea in deep-sea Fe-Mn crusts, nodules, and sediments from the Atlantic Ocean. The results showed that the microbiome of Fe-Mn deposits in the Atlantic seamounts had lower diversity and richness compared to the Pacific areas. Unique taxa and a higher abundance of potential metal-cycling bacteria were found in the crusts and nodules from Atlantic seamounts. The microbial beta-diversity was clearly grouped according to the microhabitats and geochemistry of the samples. These findings demonstrate the importance of microbial community analysis in environmental baseline studies for areas within the potential of deep-sea mining.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Infectious Diseases
Marina Maria Moraes de Seixas, Jansen de Araujo, Scott Krauss, Thomas Fabrizio, David Walker, Tatiana Ometto, Luciano Matsumiya Thomazelli, Ralph Eric Thijl Vanstreels, Renata Ferreira Hurtado, Lucas Kruger, Roberta Piuco, Maria Virginia Petry, Robert G. Webster, Richard J. Webby, Dong-Hun Lee, David Hyunjung Chung, Helena Lage Ferreira, Edison Luiz Durigon
Summary: Wild aquatic birds in Antarctic region may carry avian influenza viruses (AIVs) and have the potential of inter-species transmission.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Virology
Janaina Macedo-da-Silva, Livia Rosa-Fernandes, Vinicius de Morais Gomes, Veronica Feijoli Santiago, Deivid Martins Santos, Catarina Maria Stanischesk Molnar, Bruno Rafael Barboza, Edmarcia Elisa de Souza, Rodolfo Ferreira Marques, Silvia Beatriz Boscardin, Edison Luiz Durigon, Claudio Romero Farias Marinho, Carsten Wrenger, Suely Kazue Nagahashi Marie, Giuseppe Palmisano
Summary: We found that SARS-CoV-2 infection leads to increased protein arginylation, and inhibition of this process has potential therapeutic value.
Article
Multidisciplinary Sciences
Silvia Y. Bando, Fernanda B. Bertonha, Sandra E. Vieira, Danielle B. L. de Oliveira, Vanessa N. Chalup, Edison L. Durigon, Patricia Palmeira, Ana Cristina P. Curi, Caroline S. Faria, Leila Antonangelo, Gerhard da P. Lauterbach, Fabiane A. Regalio, Roberto M. Cesar, Carlos A. Moreira-Filho
Summary: Comparative transcriptome analysis, along with clinico-demographic and laboratory data, revealed differences in the molecular mechanisms underlying COVID-19 severity between mild and severe cases and between age subgroups. Three transcriptional modules were identified that correlated with disease traits, along with 23 differentially expressed genes associated with disease severity. These biomarkers may aid in better risk stratification for COVID-19.
SCIENTIFIC REPORTS
(2023)
Article
Virology
Carlos Roberto Prudencio, Vivaldo Gomes da Costa, Leticia Barboza Rocha, Hernan Hermes Monteiro da Costa, Diego Jose Belato Orts, Felipe Rocha da Silva Santos, Paula Rahal, Nikolas Alexander Borsato Lino, Pamela Joyce Previdelli da Conceicao, Cintia Bittar, Rafael Rahal Guaragna Machado, Edison Luiz Durigon, Joao Pessoa Araujo Jr, Juliana Moutinho Polatto, Miriam Aparecida da Silva, Joyce Araujo de Oliveira, Thais Mitsunari, Lennon Ramos Pereira, Robert Andreata-Santos, Luis Carlos de Souza Ferreira, Daniela Luz, Roxane Maria Fontes Piazza
Summary: This study successfully developed serological tests for the detection of Zika virus infection, which can enhance the sensitivity of detecting Zika virus-infected subjects.
Article
Virology
Yasmin da Silva Santos, Thais Helena Martins Gamon, Marcela Santiago Pacheco de Azevedo, Bruna Larotonda Telezynski, Edmarcia Elisa de Souza, Danielle Bruna Leal de Oliveira, Jamille Gregorio Dombrowski, Livia Rosa-Fernandes, Giuseppe Palmisano, Leonardo Jose de Moura Carvalho, Maria Cecilia Rui Luvizotto, Carsten Wrenger, Dimas Tadeu Covas, Rui Curi, Claudio Romero Farias Marinho, Edison Luiz Durigon, Sabrina Epiphanio
Summary: Since December 2019, the COVID-19 pandemic caused by SARS-CoV-2 has been affecting the world, and now multiple variants are emerging. This study compared the effects of the wild-type (Wt) (Wuhan) strain, P.1 (Gamma) variant, and Delta variant in infected K18-hACE2 mice. The P.1-infected mice showed more severe clinical manifestations and weight loss compared to the other groups. The respiratory capacity was also reduced in P.1-infected mice. Pulmonary histological findings revealed a more aggressive disease with P.1 and Delta variants compared to the Wt strain.
Article
Immunology
Ariane Nardy, Camila Tussato Soares Camargo, Yasmim Faustina Castro de Oliveira, Fernanda Cristina da Silva, Millena Soares de Almeida, Fernanda Rodrigues Monteiro, Brenda Rodrigues Silva, Jonatas Bussador do Amaral, Danielle Bruna Leal Oliveira, Edison Luiz Durigon, Guilherme Pereira Scagion, Vanessa Nascimento Chalup, Erika Donizetti Candido, Andressa Simoes Aguiar, Neil Ferreira Novo, Marina Tiemi Shio, Carolina Nunes Franca, Luiz Henrique da Silva Nali, Andre Luis Lacerda Bachi
Summary: This study investigated the impact of IFN-lambda-3 polymorphism on specific IgG responses for COVID-19 in older adults seropositive for CMV. The results suggested that both CMV seropositivity and the homozygosis for allele 1 (C/C) in IFN-lambda-3 gene can negatively impact the antibody response to COVID-19 infection and vaccination in older adults.
Article
Microbiology
Gabriela Gomes Celestino, Marla Karine Amarante, Eliana Carolina Vespero, Eliandro Reis Tavares, Lucy Megumi Yamauchi, Erika Donizetti Candido, Danielle Bruna Leal de Oliveira, Edison Luiz Durigon, Sueli Fumie Yamada-Ogatta, Ligia Carla Faccin-Galhardi
Summary: The case study presents the dermatological manifestations of COVID-19 in a patient with genetic thrombophilia and a SARS-CoV-2 variant of interest (VOI). A 47-year-old unvaccinated female patient with thrombophilia was diagnosed with COVID-19 and developed urticarial and maculopapular eruptions. Viral genome sequencing identified the VOI Zeta (P.2). The patient's symptoms and skin manifestations improved over time, indicating a correlation with the reduction in D-dimer levels.
Article
Microbiology
Fabiana Fioravante Coelho, Miriam Aparecida da Silva, Thiciany Blener Lopes, Juliana Moutinho Polatto, Natalia Salazar de Castro, Luis Adan Flores Andrade, Karine Lima Lourenco, Hugo Itaru Sato, Alex Fiorini de Carvalho, Helena Perez Coelho, Flavia Fonseca Bagno, Daniela Luz, Vincent Louis Viala, Pedro Queiroz Cattony, Bruna de Sousa Melo, Ana Maria Moro, Wagner Quintilio, Ana Paula Barbosa, Camila Gasque Bomfim, Camila Pereira Soares, Cristiane Rodrigues Guzzo, Flavio Guimaraes Fonseca, Edison Luiz Durigon, Ricardo Tostes Gazzinelli, Santuza M. Ribeiro Teixeira, Roxane Maria Fontes Piazza, Ana Paula Fernandes
Summary: SARS-CoV-2 antigen rapid diagnostic tests (Ag-RDTs) have become an important tool for pandemic control. This study describes the development and validation of an Ag-RDT prototype using a new monoclonal antibody that recognizes various SARS-CoV-2 variants, including the Omicron variant. The prototype showed high sensitivity and specificity, meeting the WHO recommendations, and may serve as an alternative tool for COVID-19 point-of-care diagnosis and epidemiological surveillance.
Article
Marine & Freshwater Biology
Paulo Y. G. Sumida, Vivian H. Pellizari, Rafael A. Lourenco, Camila N. Signori, Amanda G. Bendia, Orlemir Carrerette, Fernanda M. Nakamura, Raissa B. Ramos, Gilberto Bergamo, Bruno H. M. Souza, Ana C. A. Butarelli, Julia G. Passos, Rodolfo J. S. Dias, Mascimiliano Maly, Thomas N. S. Banha, Arthur Z. Guth, Luiza F. Soares, Paula D. N. Perugino, Felipe R. Santos, Frederico R. Santana, Michel M. de Mahiques
Summary: The article discusses the geological-geomorphological features and ecosystems in the southeastern Brazilian coast, as well as the multidisciplinary oceanographic research conducted in the region to understand the geological, chemical, and biological aspects of deep-sea habitats.
OCEAN AND COASTAL RESEARCH
(2022)
Article
Multidisciplinary Sciences
Camila T. Kinasz, Marianne G. Kreusch, Amanda G. Bendia, Vivian H. Pellizari, Rubens T. D. Duarte
Summary: Active volcanoes in Antarctica can disperse pyroclastic minerals to the surrounding glacial environment, carrying nutrients and microorganisms. This study analyzed the microbial community structure of an Antarctic glacier ice with tephra layers and found a highly diverse community dominated by Bacteroidetes, Cyanobacteria, and Proteobacteria. Functional analysis revealed complete carbon, nitrogen, and sulfur biogeochemical cycles, with potential applications in biotechnology.
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS
(2022)
