期刊
VETERINARY MICROBIOLOGY
卷 131, 期 3-4, 页码 419-423出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetmic.2008.04.012
关键词
Prototheca zopfii; Genotype; Bovine mastitis; Cow barn
资金
- Japanese Ministry of Education, Culture, Sports, Science and Technology (MEXT)
- Japanese Ministry of Agriculture, Forestry and Fisheries (MAFF)
This study is the first investigation on Japanese isolates of Prototheca zopfii from bovine mastitis and the cow-bam surroundings by molecular characterization to clarify routes of infection for bovine protothecal mastitis. We performed isolation of Prototheca from cow-bam surroundings (drinking water, sewage and feces) and milk samples from cases of bovine mastitis. Genotypes of the 32 isolates of P. zopfii from cow-barn surroundings and 67 isolates from mastitis were analyzed by genotype-specific PCR assays and restriction fragment length polymorphism (RFLP) assays. All mastitis isolates were identified as P. zopfii genotype 2. Conversely, 29 isolates from cow-barn surroundings were identified as P. zopfii genotypes I and 3 isolates as genotype 2, respectively. Given these results, both genotypes of P. zopfii could exist in cow-barn surroundings, but no sites were identified as frequent sources of P. zopfii genotype 2. P. zopfii isolates should thus be further explored with regard to genotype to clarify the reservoir of etiological agents in bovine Prototheca mastitis. (C) 2008 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据