Article
Biochemistry & Molecular Biology
Christopher H. Connor, Amanda Z. Zucoloto, John T. Munnoch, Ian-Ling Yu, Jukka Corander, Paul A. Hoskisson, Braedon McDonald, Alan Mcnally
Summary: This study demonstrates that MDR E. coli ST131 can compete and displace non-MDR E. coli in vivo, even without antibiotic treatment. The study also reveals that carriage of AMR genes is associated with increased diversity in carbohydrate metabolism genes.
Article
Microbiology
Dalmasso Guillaume, Beyrouthy Racha, Brugiroux Sandrine, Ruppe Etienne, Guillouard Laurent, Bonnin Virginie, Saint-Sardos Pierre, Ghozlane Amine, Gaumet Vincent, Barnich Nicolas, Delmas Julien, Bonnet Richard
Summary: Without the use of antibiotics, the plasmid-mediated resistance gene mcr-1 enhances the colonization ability of Escherichia coli in the gut, but impairs its lethal effect. This improvement in gut fitness is associated with a downregulation of intestinal inflammatory markers and the preservation of intestinal microbiota composition.
Article
Immunology
Regula Burkhard, Mia Koegler, Kirsty Brown, Kirsten Wilson, Lukas F. Mager, Amanda Z. Zucoloto, Carolyn Thomson, Roopa Hebbandi Nanjundappa, Isla Skalosky, Shokouh Ahmadi, Braedon McDonald, Markus B. Geuking
Summary: Healthy host-microbial mutualism with our intestinal microbiota relies on compartmentalization and regulation of adaptive mucosal and systemic anti-microbial immune responses. However, intestinal commensal bacteria can reach the systemic circulation, leading to commensal bacteremia that needs to be dealt with by the systemic immune system. Germ-free mice show increased systemic immune sensitivity and hyperreactivity to commensal Escherichia coli, indicating that commensal colonization also regulates systemic anti-commensal responses.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Microbiology
Denis K. Byarugaba, Godfrey Wokorach, Stephen Alafi, Bernard Erima, Florence Najjuka, Edison A. Mworozi, Hannah Kibuuka, Fred Wabwire-Mangen
Summary: This study characterized commensal Escherichia coli from food-producing animals in Uganda and found high genomic diversity with antimicrobial resistance and a wide range of virulence-associated genes. Cattle were the main source of E. coli carrying Shiga toxin genes, while swine were the main source of E. coli carrying colicin-like Usp toxin gene.
Article
Parasitology
Lei Deng, Jingxin Yao, Shanyu Chen, Tingmei He, Yijun Chai, Ziyao Zhou, Xiaogang Shi, Haifeng Liu, Zhijun Zhong, Hualin Fu, Guangneng Peng
Summary: The study investigated the prevalence and subtype distribution of Blastocystis sp. in captive wildlife in zoos in Sichuan Province, southwestern China, revealing a high prevalence of infection among the animals, with Panzhihua Zoo having the highest prevalence. Subtype analysis identified nine subtypes, including six zoonotic and three animal-specific subtypes, with ST17 being the predominant subtype in Blastocystis sp.-positive isolates. This study suggests that captive wildlife in zoos may serve as reservoirs for human Blastocystis sp. infections.
PARASITES & VECTORS
(2021)
Article
Infectious Diseases
Javier Caballero-Gomez, Ignacio Garcia-Bocanegra, David Cano-Terriza, Adrian Beato-Benitez, Rainer G. Ulrich, Javier Martinez, Rafael Guerra, Rosa Martinez-Valverde, Eva Martinez-Nevado, Miguel Angel Quevedo-Munoz, Cecilia Sierra-Arqueros, Javier Planas, Noelia de Castro-Garcia, Antonio Rivero, Antonio Rivero-Juarez
Summary: In recent years, exposure to hepatitis E virus (HEV) has been found in zoo animals in Spain. The study shows a higher seropositivity rate in carnivores and perissodactyls, as well as a higher infection rate during the period of 2012-2016. Further research is needed to determine the role of zoo species in the epidemiology of HEV.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Microbiology
Xueliang Zhao, Yunyang Lv, Fathalrhman Eisa Addoma Adam, Qingfang Xie, Bin Wang, Xindong Bai, Xiaoyuan Wang, Honghu Shan, Xinglong Wang, Haijin Liu, Ruyi Dang, Juan Wang, Zengqi Yang
Summary: Pathogenic E. coli in sheep from large-scale farms in China exhibit differences in antimicrobial resistance, virulence genes, and biofilm formation. Free-range sheep have fewer antibiotic-resistant strains but higher levels of virulence genes and biofilm formation compared to intensively farmed sheep.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Marta Ksiazczyk, Bartlomiej Dudek, Maciej Kuczkowski, Robert O'Hara, Kamila Korzekwa, Anna Wzorek, Agnieszka Korzeniowska-Kowal, Mathew Upton, Adam Junka, Alina Wieliczko, Radoslaw Ratajszczak, Gabriela Bugla-Ploskonska
Summary: This study compared E. coli populations isolated from reptiles, birds, and mammals (humans) for the first time, revealing that most reptilian E. coli strains are nonpathogenic with a low frequency of virulence gene occurrence, differing from the uropathogenic and avian pathogenic strains. Additionally, phylogroups distribution was relatively homogeneous among tested mammalian and avian strains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Infectious Diseases
Timea Kocurekova, Livia Karahutova, Dobroslava Bujnakova
Summary: The study revealed that commercial broilers can act as reservoirs of virulent and resistant genes, in addition to E. coli strains causing (extra-)intestinal infections, posing a potential threat to human health through direct contact and foodborne transmission.
Article
Multidisciplinary Sciences
Bin Liu, Lingyan Jiang, Yutao Liu, Hongmin Sun, Jun Yan, Chenbo Kang, Bin Yang
Summary: The mammalian gastrointestinal tract is a complex environment that hosts a diverse microbial community. This study discovered that enterohemorrhagic Escherichia coli (EHEC) O157:H7 imports L-malate from the host and microbiota using the DcuABC transporters, converting it into fumarate to fuel anaerobic respiration and promote colonization. L-malate also acts as a signaling molecule to activate virulence gene expression in EHEC O157:H7.
NATURE COMMUNICATIONS
(2023)
Article
Materials Science, Multidisciplinary
Sadaf Tariq, Sobia Tabassum, Sadia Aslam, Mika Sillanpaa, Wahidah H. Al-Qahtani, Shafaqat Ali
Summary: This study found that drinking water from different sources is contaminated with potential DEC pathotypes, which may cause diarrheal diseases. PCR analysis showed that some samples carry virulence genes, with eaeA and sth genes being the most commonly detected.
Article
Immunology
Uriel A. Angulo-Zamudio, Javier Gutierrez-Jimenez, Luis Monroy-Higuera, Hector Flores-Villasenor, Nidia Leon-Sicairos, Jorge Velazquez-Roman, Jorge E. Vidal, Gabriela Tapia-Pastrana, Adrian Canizalez-Roman
Summary: Escherichia coli strains, including diarrheagenic E. coli (DEC), are significant causes of childhood diarrhea in developing countries. The study revealed that the presence of E. coli strains with SVG, regardless of being DEC or non-DEC, may be associated with diarrhea in Mexican children.
MICROBIAL PATHOGENESIS
(2021)
Article
Microbiology
Monika Michalecka, Joanna Pulawska
Summary: This study analyzed the relationship between housekeeping and pathogenicity-related genes and virulence towards primary Malus resistance genes in Venturia inaequalis fungus, the causal agent of apple scab. The results showed that the analyzed genes may be too conserved to reflect the adaptation of pathogens to different apple genotypes with resistance genes, and genetic divergence was observed among strains virulent to apple trees containing specific resistance genes. The study confirmed a lack of free recombination between strains, indicating non-random polymorphisms associated with the strains analyzed.
Article
Agriculture, Dairy & Animal Science
Adriana Yanez-Villa, Rosa I. Martinez-Flores, Efren Diaz-Aparicio, Erika M. Carrillo-Casas, Rosario Morales-Espinosa, Gabriela Delgado, Armando Navarro, Sara Arroyo-Escalante, Juan Xicohtencatl-Cortes, Francisco Aguilar-Romero, Rigoberto Hernandez-Castro
Summary: This study aimed to determine the clonality of 41 strains of Escherichia coli isolated from diarrheal goat kids using PFGE. PCR amplification was used to detect the presence of virulence genes associated with various diarrheagenic pathotypes. The results showed a heterogeneous population of E. coli with a predominance of virulence genes from STEC and ETEC pathotypes. The most frequent phylogenetic groups were B1 and A.
SMALL RUMINANT RESEARCH
(2023)
Article
Immunology
Diana Tilevik, Anna-Karin Pernestig, Magnus Fagerlind, Andreas Tilevik, Lars Ljungstrom, Markus Johansson, Helena Enroth
Summary: In this study, we used whole-genome sequencing to conduct a comprehensive genotypic characterization of ExPEC isolates. Our findings showed that ESBL-producing isolates had a higher number of plasmids, virulence genes, and resistance genes compared to non-ESBL-producing isolates, although this difference was not statistically significant due to the small sample size. Additionally, we observed a diversity of sequence types and a predominance of phylogroup A in both ESBL-producing and non-ESBL-producing isolates.
MICROBIAL PATHOGENESIS
(2022)
Article
Environmental Sciences
Ewa Bok, Aleksandra Kozanska, Justyna Mazurek-Popczyk, Magdalena Wojciech, Katarzyna Baldy-Chudzik
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2020)
Article
Infectious Diseases
Justyna Mazurek-Popczyk, Justyna Pisarska, Ewa Bok, Katarzyna Baldy-Chudzik
Review
Immunology
Michal Stosik, Beata Tokarz-Deptula, Jakub Deptula, Wieslaw Deptula
FRONTIERS IN IMMUNOLOGY
(2020)
Review
Medicine, General & Internal
Justyna Pisarska, Katarzyna Baldy-Chudzik
JOURNAL OF CLINICAL MEDICINE
(2020)
Article
Fisheries
Michal Stosik, Beata Tokarz-Deptula, Wieslaw Deptula
Summary: Interferons are vital proteins in the immune response, found in various animals including ray-finned fish. In Actinopterygii, cytokines like IFN I and IFN II primarily exhibit antiviral activity through the JAK-STAT signaling pathway, while also participating in antimicrobial responses in some fish species.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Fisheries
Michal Stosik, Beata Tokarz-Deptula, Wieslaw Deptula
Summary: Immunological memory plays a key role in adaptive immunity, leading to a faster response upon re-exposure to the same antigen. Genetic alterations may maintain immune activity at a low level independently of the antigen. Studies in Teleostei suggest the presence of immunological memory determined by T and B cells, with potential involvement of macrophages and NK-like cells.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Review
Immunology
Beata Tokarz-Deptula, Joanna Palma, Lukasz Baraniecki, Michal Stosik, Roman Kolacz, Wieslaw Deptula
Summary: Platelets play a role in inflammation and infections by reacting with the endovascular environment and cells of the immune system. This role is influenced by biologically active substances present in platelet granules and EV structures.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Critical Care Medicine
Justyna Mazurek-Popczyk, Lukasz Palka, Katarzyna Arkusz, Bartosz Dalewski, Katarzyna Baldy-Chudzik
Summary: Additive manufacturing has enabled the development of personalized medical devices. In this study, the topography of 3D-printed fracture fixation plates and their susceptibility to biofilm development were compared with commercially available plates. The results showed that 3D-printed plates had higher roughness and were more densely colonized by bacteria.
INJURY-INTERNATIONAL JOURNAL OF THE CARE OF THE INJURED
(2022)
Review
Immunology
Michal Stosik, Beata Tokarz-Deptula, Wieslaw Deptula
Summary: This review presents Danio rerio as a model organism for studying hematopoiesis in vertebrates and discusses the development of this process in fish and mammals, as well as the impact of microenvironmental factors.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jagoda Kurowiak, Agnieszka Kaczmarek-Pawelska, Agnieszka Mackiewicz, Katarzyna Baldy-Chudzik, Justyna Mazurek-Popczyk, Lukasz Zareba, Tomasz Klekiel, Romuald Bedzinski
Summary: This article introduces the potential of new hydrogel materials in medical applications, focusing on the study of mechanical properties and antimicrobial activity. The study found that adding gelatin to sodium alginate-based hydrogels reduces their mechanical properties and accelerates degradation. Moreover, the presence of African plum bark extract enhances the inhibitory effect of the hydrogel on bacteria.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Dentistry, Oral Surgery & Medicine
Justyna Mazurek-Popczyk, Adam Nowicki, Katarzyna Arkusz, Lukasz Palka, Anna Zimoch-Korzycka, Katarzyna Baldy-Chudzik
Summary: This study aimed to assess the microbial adhesion on acrylamide resins used for temporary restorations in 3D printing technology and evaluate the impact of post-processing. The results showed that the tested resins were susceptible to biofilm formation by oral streptococci, staphylococci, and Candida. Post-processing, particularly glazing, significantly reduced bacterial biofilm formation and the risk of failure of the final restoration.
Article
Medicine, Research & Experimental
Aleksandra Kozanska, Katarzyna Baldy-Chudzik
Summary: HPV is a common causative agent of human infections, with some types posing serious health risks such as cervical cancer. The virus replication cycle is intricately linked to the differentiation of mucosal epithelial cells, requiring different replication mechanisms at various stages. Understanding these mechanisms is crucial for developing effective therapeutic approaches against HPV-related diseases.
POSTEPY HIGIENY I MEDYCYNY DOSWIADCZALNEJ
(2021)
Article
Nanoscience & Nanotechnology
Katarzyna Arkusz, Ewa Paradowska, Marta Nycz, Justyna Mazurek-Popczyk, Katarzyna Baldy-Chudzik
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
(2020)
Review
Veterinary Sciences
Michal Stosik, Beata Tokarz-Deptula, Wieslaw Deptula
JOURNAL OF VETERINARY RESEARCH
(2020)
Article
Microbiology
Lukasz Palka, Justyna Mazurek-Popczyk, Katarzyna Arkusz, Katarzyna Baldy-Chudzik
JOURNAL OF ORAL MICROBIOLOGY
(2020)