Article
Biology
Maria Alfonsa Cavalera, Andrea Zatelli, Rossella Donghia, Jairo Alfonso Mendoza-Roldan, Floriana Gernone, Domenico Otranto, Roberta Iatta
Summary: This study found that conjunctival swab (CS) real-time polymerase chain reaction (qPCR) had unsatisfactory diagnostic and prognostic performance for canine leishmaniosis (CanL). Even with highly sensitive qPCR detecting Leishmania infantum kDNA, it should be accompanied by significant clinical score for CanL and/or other direct diagnostic tests positivity. In seropositive dogs with CS qPCR positivity, sampling time and season variability of results should be considered.
Article
Infectious Diseases
Yun Wu, Mengyuan Jiang, Shaogang Li, Nicholas R. Waterfield, Guowei Yang
Summary: The study established a rapid and accurate Leishmania species identification method using real-time PCR, which can directly use clinical samples for identification, and has important implications for clinical diagnosis and patient management.
INFECTIOUS DISEASES OF POVERTY
(2022)
Article
Microbiology
Olga Lucia Fernandez, Mariana Rosales-Chilama, Natali Quintero, Bruno L. Travi, Dawn M. Wetzel, Maria Adelaida Gomez, Nancy Gore Saravia
Summary: Combination therapy with posaconazole and miltefosine shows synergistic activity against Leishmania panamensis infection, suggesting its potential as a novel therapeutic strategy to improve treatment efficacy and reduce drug resistance.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Parasitology
Zahra Bahramdoost, Hamed Mirjalali, Parvin Yavari, Ali Haghighi
Summary: Stool samples from dogs and humans were examined for Giardia lamblia infection using parasitological and molecular techniques. The prevalence of assemblages A and B in humans was 55.8% and 14.7% respectively, with 14.7% of samples showing mixed infections. HRM real-time PCR was more effective than PCR/sequencing in detecting infections in both dogs and humans.
Article
Multidisciplinary Sciences
Jennifer J. Labisch, G. Philip Wiese, Kalpana Barnes, Franziska Bollmann, Karl Pflanz
Summary: The study developed a real-time imaging method for quantifying the infectious titer of lentiviral vectors, which showed lower standard deviation and a broader linear range compared to traditional methods, enabling real-time titer results.
Article
Medicine, General & Internal
Juliana B. T. Carnielli, Anuja Dave, Audrey Romano, Sarah Forrester, Pedro R. de Faria, Renata Monti-Rocha, Carlos H. N. Costa, Reynaldo Dietze, Ian A. Graham, Jeremy C. Mottram
Summary: In Brazil, miltefosine treatment failure in visceral leishmaniasis has been linked to the deletion of the miltefosine susceptibility locus (MSL) in Leishmania infantum. CRISPR-Cas9 was used to investigate the role of NUC1, NUC2, HLP, and TEI in miltefosine resistance mechanisms. Findings suggest that the absence of NUC1 and NUC2 from the MSL contributes to natural resistance to miltefosine in L. infantum.
Article
Entomology
Maria S. Latrofa, Jairo A. Mendoza-Roldan, Ranju R. S. Manoj, Marco Pombi, Filipe Dantas-Torres, Domenico Otranto
Summary: Leishmanioses are vector-borne diseases transmitted by sand flies, and a new duplex quantitative real-time PCR was developed to detect and differentiate two Leishmania species. The study found the presence of Leishmania in sand flies and reptiles, highlighting the potential of the new PCR method in diagnosing Leishmaniasis.
ENTOMOLOGIA GENERALIS
(2021)
Article
Chemistry, Analytical
Teruaki Tozaki, Aoi Ohnuma, Shinichi Iwai, Mio Kikuchi, Taichiro Ishige, Hironaga Kakoi, Keiichi Hirota, Kanichi Kusano, Shunichi Nagata
Summary: The study investigated the robustness of digital PCR and real-time PCR in transgene detection. Digital PCR was relatively robust when primer and probe sequences did not completely match the template transgene, while real-time PCR was less sensitive. Lowering the annealing temperature can improve PCR sensitivity.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Bong Kyun Kim, Sang-A Lee, Minju Park, Eui Ju Jeon, Mi Jung Kim, Jung Min Kim, Heesuk Kim, Seungwon Jung, Sang Kyung Kim
Summary: This article introduces a photonic PCR platform using hydrogel microparticles, which utilizes photothermal nanomaterials as heating elements for rapid and portable POCT. The researchers successfully demonstrate the ability to perform multiplex assays by loading multiple encoded pPIN microparticles in a single reaction.
Article
Biochemistry & Molecular Biology
Estela Melcon-Fernandez, Giulio Galli, Carlos Garcia-Estrada, Rafael Balana-Fouce, Rosa M. Reguera, Yolanda Perez-Pertejo
Summary: Visceral leishmaniasis is a neglected tropical disease caused by Leishmania parasites, which is prevalent in East Africa, Asia, South America, and the Mediterranean Basin. The current pharmacological control of the disease is limited by the availability of old, poorly adherent drugs with adverse effects and low oral bioavailability. Resistance to first-line drugs has emerged due to misuse. However, recent research has identified nifuratel, a drug used for vaginal infections, as a promising candidate for repurposing due to its efficacy against leishmaniasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Medicinal
Caroline R. Espada, Andreia Albuquerque-Wendt, Valentin Hornillos, Eva Gluenz, Adriano C. Coelho, Silvia R. B. Uliana
Summary: The ros3 gene copy number was found to be associated with increased miltefosine uptake and susceptibility in Leishmania. Modulating the gene copy number showed significant impact on miltefosine susceptibility, with overexpression leading to increased EC50 values and deletion resulting in higher EC50 values. This study confirms that ros3 copy number is a factor affecting differential susceptibility and uptake of miltefosine.
ACS INFECTIOUS DISEASES
(2021)
Article
Public, Environmental & Occupational Health
Jaime Soto, Patricia Gutierrez, Paula Soto, David Paz, Eduardo Cayhuara, Carmen Molina, Mia Sanchez, Jonathan Berman
Summary: This study provides valuable guidance on the treatment efficacy and adverse events of Leishmania braziliensis infection based on limited clinical data from a hospital in Bolivia. The cure rates are relatively high, but new drugs are still needed to improve chemotherapy outcomes.
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2022)
Review
Veterinary Sciences
Sharmaine L. Miller, James A. Serpell, Kathryn R. Dalton, Kaitlin B. Waite, Daniel O. Morris, Laurel E. Redding, Nancy A. Dreschel, Meghan F. Davis
Summary: Investigations of therapy dog welfare have primarily focused on physiological and behavioral measures, but have not fully considered the continuum of welfare. This review proposes using objective measurements of the HPA axis and behavioral observations to evaluate positive welfare in therapy dogs. The role of oxytocin and questionnaire data are also discussed. However, to date, no studies have comprehensively measured these factors to assess therapy dog welfare.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Veterinary Sciences
Mahdi Fakhar, Majid Derakhshani-nia, Shaban Gohardehi, Mehdi Karamian, Hajar Ziaei Hezarjaribi, Mehdi Mohebali, Behnaz Akhoundi, Mitra Sharbatkhori
Summary: The aim of this study was to determine the Leishmania species infecting domestic dogs in Golestan Province, Iran. The results showed that 7% of the dogs tested positive for antibodies and 25% tested positive for Leishmania spp. DNA by PCR, with most of the dogs being asymptomatic. PCR testing also revealed a significantly higher infection rate in male dogs compared to female dogs. Additionally, a new emerging agent for Leishmania infection was identified.
VETERINARY MEDICINE AND SCIENCE
(2022)
Article
Microbiology
Caroline Ricce Espada, Erica V. de Castro Levatti, Mariana Cortes Boite, Dorcas Lamounier, Jorge Alvar, Elisa Cupolillo, Carlos Henrique Nery Costa, Joelle Rode, Silvia R. B. Uliana
Summary: This study investigated the susceptibility of 73 Brazilian strains of Leishmania infantum to miltefosine, showing relatively low heterogeneity in miltefosine susceptibility. The results suggest that Brazilian L. infantum may not have decreased susceptibility to miltefosine, indicating the potential for future clinical evaluation of miltefosine for VL treatment in Brazil.
Article
Chemistry, Applied
Angela Salzano, Gianluca Neglia, Nunzia D'Onofrio, Maria Luisa Balestrieri, Antonio Limone, Alessio Cotticelli, Raffaele Marrone, Aniello Anastasio, Michael J. D'Occhio, Giuseppe Campanile
Summary: The study demonstrated that adding green feed to the diet of dairy buffaloes enhances the content of health-promoting biomolecules and the antioxidant and antineoplastic properties in milk. Treated buffaloes had higher levels of L-carnitine biomolecules in their milk and showed greater antioxidant activity. Additionally, milk from the treated buffaloes exhibited stronger potency in inhibiting the viability of cancer cells.
Article
Agriculture, Dairy & Animal Science
Angela Salzano, Sara Damiano, Livia D'Angelo, Gabriele Ballistreri, Salvatore Claps, Domenico Rufrano, Aristide Maggiolino, Gianluca Neglia, Pasquale De Palo, Roberto Ciarcia
Summary: The study demonstrates that supplementing the diet with RLE rich in anthocyanins is effective in improving meat quality, reducing oxidative levels, enhancing color and fatty acid profile, ultimately producing healthier meat for human consumption.
Article
Agriculture, Dairy & Animal Science
Vincenzo Landi, Aristide Maggiolino, Angela Salzano, Salvatore Claps, Pasquale De Palo, Domenico Rufrano, Giuseppina Pedota, Gianluca Neglia
Summary: The study compared the application of Wood's model to different test-day milk recording protocols for estimating total milk, fat, and protein yield in dairy goats, showing that the Wood model applied to all 14 recordings yielded results very close to applying the model on test-day records every 15 days.
Article
Agriculture, Dairy & Animal Science
A. Maggiolino, A. Bragaglio, A. Salzano, D. Rufrano, S. Claps, L. Sepe, S. Damiano, R. Ciarcia, F. R. Dinardo, D. L. Hopkins, G. Neglia, P. De Palo
Summary: The study showed that the addition of red orange and lemon extract rich in anthocyanins in suckling lambs can reduce cooking loss and shear force values in meat, as well as lower the levels of oxidized substances. Additionally, the meat from lambs supplemented with RLE exhibited better yellowness values and antioxidant profile during aging compared to the control group.
ANIMAL FEED SCIENCE AND TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Nunzio Antonio Cacciola, Angela Salzano, Nunzia D'Onofrio, Tommaso Venneri, Paola De Cicco, Francesco Vinale, Orsolina Petillo, Manuela Martano, Paola Maiolino, Gianluca Neglia, Ciro Campanile, Lorella Severino, Carmine Merola, Francesca Borrelli, Maria Luisa Balestrieri, Giuseppe Campanile
Summary: Pharmacological research on milk whey has shown its potential therapeutic properties in modern medicine. This study focused on whey from Mediterranean buffalo milk and investigated its anticancer effects on colorectal cancer. The results revealed that whey was able to regulate protein expression in apoptotic and necroptotic pathways to inhibit tumor growth. The findings suggest that whey could be a valuable source of biomolecules for the treatment of colorectal cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Green & Sustainable Science & Technology
Roberto Chirone, Andrea Paulillo, Piero Salatino, Angela Salzano, Brigida Cristofaro, Teresa Cristiano, Giuseppe Campanile, Gianluca Neglia
Summary: This study evaluates the environmental impacts of buffalo milk production in southern Italy using the Life Cycle Assessment approach. The analysis finds that the environmental impacts are highly dependent on the allocation method and that the milk productivity of buffalos is a key factor in determining the environmental performance of each farm.
JOURNAL OF CLEANER PRODUCTION
(2022)
Article
Agriculture, Dairy & Animal Science
Roberta Matera, Gabriele Di Vuolo, Alessio Cotticelli, Angela Salzano, Gianluca Neglia, Roberta Cimmino, Danila D'Angelo, Stefano Biffani
Summary: The study aimed to investigate the relationships between milk electrical conductivity (EC), production traits, and somatic cell count in Italian Mediterranean Buffalo. The results showed a strong relationship between EC and somatic cells, with frequent EC recording proving more effective for early mastitis detection. Further studies are needed to validate these findings for the development of udder disease prediction models.
Article
Agriculture, Dairy & Animal Science
T. Bobbo, R. Matera, G. Pedota, A. Manunza, A. Cotticelli, G. Neglia, S. Biffani
Summary: Mastitis has negative impacts on the dairy industry worldwide, affecting animal health, milk production and quality, as well as farmers' income. Machine learning analysis was applied in this study to predict subclinical mastitis in Italian Mediterranean buffaloes, using data collected during routine milk recording procedures and climatic data.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
Gianluca Neglia, Serena Calabro, Alessio Cotticelli, Angela Salzano, Roberta Matera, Alessandro Vastolo, Nunzia D'Onofrio, Andrea Giorgino, Elisa Martino, Maria L. Balestrieri, Giuseppe Campanile
Summary: The inclusion of former food products in the diet of buffaloes as an alternative to green forage did not significantly affect fermentation characteristics, milk yield and quality, and the content of certain biomolecules and total antioxidant activity. Therefore, biscuit meal can be used as a substitute for green fodder to ensure environmental sustainability and optimize costs without compromising milk quality.
JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION
(2023)
Article
Agriculture, Dairy & Animal Science
Roberta Matera, Lorenzo Pascarella, Alessio Cotticelli, Giuseppe Conte, Alessia Tondo, Giuseppe Campanile, Gianluca Neglia
Summary: The study aimed to investigate the effects of different milking machine setting parameters on the milking performance of Italian Mediterranean buffaloes. The results showed that incorrect milking settings can significantly impact milking yield and milk quality in buffaloes. Specific milking parameters are crucial for achieving optimal milking performance in buffaloes.
ITALIAN JOURNAL OF ANIMAL SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
Alessio Cotticelli, Maria Teresa Verde, Roberta Matera, Isabella Pividori, Alberto Prandi, Gianluca Neglia, Tanja Peric
Summary: This study validated a radioimmunoassay method to measure cortisol levels in buffalo milk, which can be used to assess the welfare and stress levels of dairy ruminants. The results showed that the method had good precision, sensitivity, and specificity, making it suitable for cortisol measurement in buffalo milk. The described ranges can be employed in calibrating biosensing technologies integrated directly into milking parlour systems.
ITALIAN JOURNAL OF ANIMAL SCIENCE
(2022)
Article
Veterinary Sciences
Giorgio A. Presicce, Domenico Vistocco, Massimo Capuano, Luigi Navas, Angela Salzano, Giovanna Bifulco, Giuseppe Campanile, Gianluca Neglia
Summary: This study evaluated the effects of two different synchronization protocols in different seasons on primiparous buffaloes. The results showed that the progesterone-based treatment was more effective in increasing the pregnancy rate after the initial AI, and that AI efficiency after re-synchronization through progesterone treatment was higher than the Ovsynch protocol.
VETERINARY SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Alessandro Vastolo, Roberta Matera, Francesco Serrapica, Monica I. Cutrignelli, Gianluca Neglia, Dieu Donne Kiatti, Serena Calabro
Summary: This study evaluated the fermentation characteristics and kinetics of different types of liquid feed using bovine and buffalo rumen liquor as inocula. The results showed that supplementing liquid feed improved fermentation and had no negative effects.
FERMENTATION-BASEL
(2022)
Article
Agriculture, Dairy & Animal Science
Roberta Matera, Alessio Cotticelli, Mayra Gomez Carpio, Stefano Biffani, Francesco Iannacone, Angela Salzano, Gianluca Neglia
Summary: The study reveals the significant impact of temperature-humidity index (THI) on milk yield, fat percentage, and somatic cell count in buffaloes, especially in primiparous buffaloes. Unfavorable effects were observed in primiparous buffaloes at THI values lower than 62. Overall, a positive relationship between THI and milk yield was observed.
ITALIAN JOURNAL OF ANIMAL SCIENCE
(2022)
Proceedings Paper
Computer Science, Artificial Intelligence
Alessandra Amato, Flora Amato, Leopoldo Angrisani, Leonard Barolli, Francesco Bonavolonta, Gianluca Neglia, Oscar Tamburis
Summary: This study aims to apply artificial intelligence techniques in the field of precise livestock farming, specifically focusing on the identification of mastitis using non-invasive infrared techniques and early detection through deep learning. The experimental results demonstrate the effectiveness of infrared thermography measurement in early detection of mastitis in buffalo populations.
ADVANCES IN INTELLIGENT NETWORKING AND COLLABORATIVE SYSTEMS (INCOS-2021)
(2022)
Article
Veterinary Sciences
L. Alibrandi, R. Tognetti, O. Domenech, M. Croce, M. Giuntoli, G. Grosso, T. Vezzosi
Summary: This study assessed the feasibility and diagnostic reliability of a new smartphone-based ECG device in dogs, and found no significant differences compared to a traditional ECG device. The results suggest that the smartphone-based device is clinically reliable for assessing heart rate and rhythm in dogs.
VETERINARY JOURNAL
(2024)