Article
Immunology
Luiza Aymee, Maria Isabel Nogueira Di Azevedo, Ana Luiza dos Santos Baptista Borges, Filipe Anibal Carvalho-Costa, Walter Lilenbaum
Summary: This study performed a molecular analysis of Leptospira spp. from genital and urine samples of cows with Bovine Genital Leptospirosis (BGL), revealing high genetic diversity and emphasizing the importance of genital samples for diagnosing cows with reproductive failures.
MICROBIAL PATHOGENESIS
(2022)
Article
Chemistry, Analytical
Wanwisa Deenin, Abdulhadee Yakoh, Chahya Kreangkaiwal, Orawon Chailapakul, Kanitha Patarakul, Sudkate Chaiyo
Summary: In this study, an integrated lateral-flow immunoassay with an electrochemical readout (eLFIA) was developed for early-stage detection of LipL32, a protein present only in pathogenic Leptospira species. The eLFIA showed superior performance in terms of a low detectable limit and a broad linear dynamic range. The test strip provided a straightforward and sensitive approach for the diagnosis of leptospirosis.
ANALYTICAL CHEMISTRY
(2022)
Article
Agriculture, Dairy & Animal Science
Yuri Almeida Gomes, L. S. Medeiros, Maria Isabel Nogueira Di Azevedo, Ana Paula Loureiro, Juliana dos Santos Loria de Melo, Filipe Anibal Carvalho-Costa, Walter Lilenbaum
Summary: Genital leptospirosis in pigs can lead to economic losses and reproductive failures. This study identified and analyzed leptospires in the reproductive tracts of female pigs slaughtered in the Amazon region. The results suggest the possibility of venereal transmission and highlight the potential impact of genital leptospirosis syndrome on swine production.
ANIMAL REPRODUCTION SCIENCE
(2022)
Article
Biochemical Research Methods
Chintana Chirathaworn, Weena Janwitthayanan, Yupin Suputtamongkol, Yong Poovorawan
Summary: The study found that both ColA and LipL32 proteins showed high sensitivity and specificity in detecting antibodies to Leptospira, with ColA protein demonstrating even higher specificity than LipL32. This suggests that ColA protein could be a promising candidate antigen for antibody detection in leptospirosis diagnosis.
JOURNAL OF IMMUNOLOGICAL METHODS
(2021)
Article
Chemistry, Multidisciplinary
Rupak Nagraik, Sunil Sethi, Avinash Sharma, Deepak Kumar, Dinesh Kumar, Avvaru Praveen Kumar
Summary: An ultrasensitive nanohybrid electrochemical DNA sensor was developed for detecting the LipL32 gene of Leptospira interrogans, showing high sensitivity and specificity with a lower limit of detection of 0.112 pg/mu l. The sensor exhibited good reproducibility over a 6-month period, making it suitable for routine analysis in the identification of L. interrogans and diagnosis of leptospirosis.
Article
Veterinary Sciences
Felipe D. 'Azeredo Torres, Ana Luiza dos Santos Baptista Borges, Cristiane Kolesnikovas, Camila Domit, Carla Beatriz Barbosa, Filipe Anibal Carvalho-Costa, Maria Isabel Nogueira Di Azevedo, Walter Lilenbaum
Summary: This study aimed to investigate the role of pinnipeds as carriers of Leptospira sp. DNA from kidney samples of 47 pinnipeds were analyzed, and pathogenic Leptospira sp. DNA was detected in 31.9% of the samples. Eight strains were identified as L. interrogans, closely related to sequences from the Icterohaemorrhagiae serogroup. The results demonstrate that pinnipeds can act as carriers and play an important role in the dynamics of leptospirosis.
RESEARCH IN VETERINARY SCIENCE
(2023)
Article
Nanoscience & Nanotechnology
Pothiaraj Govindan, Packiyadass Manjusha, Konda Mani Saravanan, Vijayakumar Natesan, Saleh H. Salmen, Saleh Alfarraj, Milton Wainwright, Harshavardhan Shakila
Summary: The study investigated the over expression of LipL32 and hGMCSF genes in a yeast expression system to obtain a high yield of recombinant protein production. By using a novel protein expression system and protein purification method, the study confirmed the potential for enhancing vaccine effectiveness against leptospirosis through protein-protein interactions.
APPLIED NANOSCIENCE
(2021)
Article
Microbiology
Stephanie Blanchard, Carine Cariou, Jerome Bouvet, William Valfort, Frantz Oberli, Severine Villard, Florence Barret-Hilaire, Herve Poulet, Lionel Cupillard, Blandine de Saint-Vis
Summary: The efficacy of Leptospira canine vaccines was evaluated by comparing the detection of Leptospira in blood and urine samples using species-specific real-time quantitative PCR (qPCR) with the reference culture method. The results showed strong agreement between the two methods for both blood and urine samples, with no false-negative results with the qPCR assay. However, there was a statistically significant difference between blood and urine samples in terms of agreement.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Review
Infectious Diseases
Lili Hao, Chaoxi Chen, Keith Bailey, Leyi Wang
Summary: Bovine kobuvirus (BKV), a non-enveloped RNA virus, has been detected in 13 countries across four continents, potentially causing neonatal calf diarrhoea. Further research, including animal challenge studies, is urgently needed to understand the pathogenicity of BKV.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Veterinary Sciences
Junzhen Chen, Dan Li, Yafang Xu, Zeyu Li, Siqi Ma, Xinyi Liu, Yuanyuan Yuan, Chengyuan Zhang, Qiang Fu, Huijun Shi
Summary: This study optimized dual and multiplex ddPCR methods for the detection of bovine enterovirus, bovine coronavirus, and bovine rotavirus infections. The results showed that these methods were more effective than conventional qPCR methods.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Veterinary Sciences
Camila Hamond, Karen LeCount, Ellie J. Putz, Darrell O. Bayles, Patrick Camp, Marga G. A. Goris, Hans van der Linden, Nathan E. Stone, Linda K. Schlater, Jason W. Sahl, David M. Wagner, Jarlath E. Nally
Summary: This study reports the isolation of L. borgpetersenii serovar Tarassovi from the urine of a dairy cow in the U.S. for the first time. The isolate was characterized using whole-genome sequencing, serotyping, MALDI, and immunoblotting. This finding highlights the importance of culture, genotyping, and serotyping for accurate classification of leptospires and the design of effective vaccine and diagnostic strategies.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Microbiology
Luiza Aymee, Maria Isabel Nogueira Di Azevedo, Juliana de Souza Pedrosa, Juliana dos Santos Loria de Melo, Filipe Anibal Carvalho-Costa, Walter Lilenbaum
Summary: This study aimed to reinforce the importance of L. santarosai strain Guaricura as a BGL agent through genetic characterization. The isolation of this strain from the uterus of a live subfertile cow provides substantial evidence of its role as an important BGL agent.
VETERINARY MICROBIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Giusi Macaluso, Alessandra Torina, Valeria Blanda, Annalisa Guercio, Antonio Lastra, Ilenia Giacchino, Rosalia D'Agostino, Carmela Sciacca, Mario D'Incau, Cristina Bertasio, Francesca Grippi
Summary: This study investigated the prevalence and diversity of Leptospira spp. in slaughtered pigs in southern Italy (Sicily). The results showed that Leptospira infection is common among these pigs and may pose an occupational risk for humans. The study provides important insights into swine leptospiral infections and emphasizes the importance of strengthening control measures.
Article
Multidisciplinary Sciences
Ines Armendariz, Pablo A. Ferrari, Daniel Fraiman, Jose M. Martinez, Hugo G. Menzella, Silvina Ponce Dawson
Summary: The use of droplet digital PCR has been shown to be more sensitive than RT-qPCR, allowing for direct quantification of RNA content and optimizing strategies for better identification of infected individuals.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Collins Chimezie Udechukwu, Caleb Ayuba Kudi, Paul Ayub Abdu, Paul H. Mamman, Nicholas Nathaniel Pilau, Elmina Abiba Abiayi, Kelvin Olutimilehin Jolayemie, Magdalene Ogbonneya Okoronkwo, Adewole Augustine Adekola
Summary: This study found that cattle may serve as a significant reservoir of leptospirosis in the study area, posing a potential risk to abattoir workers, veterinarians, and local communities.
COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES
(2023)
Review
Immunology
Adriana Alves Oliveira Paim, Agata Lopes-Ribeiro, Daniele S. O. Daian e Silva, Luis Adan F. Andrade, Thais F. S. Moraes, Edel F. Barbosa-Stancioli, Flavio Guimaraes da Fonseca, Jordana G. Coelho-dos-Reis
Summary: COVID-19, caused by SARS-CoV-2, has become a major public health crisis with rapid transmission and multifactorial disease progression. Understanding the immune response and viral invasion mechanisms is crucial for COVID-19 management.
IMMUNOLOGY LETTERS
(2021)
Article
Biotechnology & Applied Microbiology
Silvia Giannattasio-Ferraz, Adriana Ene, Genevieve Johnson, Laura Maskeri, Andre Penido Oliveira, Swarnali Banerjee, Edel F. Barbosa-Stancioli, Catherine Putonti
Summary: Klebsiella variicola is an opportunistic pathogen in humans and is associated with bovine mastitis. The presence of this bacterium in the urine of healthy cattle raises concerns about its transmission and antibiotic resistance. The genomes of these isolates show similarity to those from the human urinary tract, suggesting niche specialization.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Daniel Goncalves Chaves, Leonardo Camilo de Oliveira, Maria Clara Fernandes da Silva Malta, Isabelle Rocha de Oliveira, Edel Figueiredo Barbosa-Stancioli, Mauro Martins Teixeira, Marina Lobato Martins
Summary: This study aimed to profile the immune response and viral neutralization capacity of individuals with IgG anti-SARS-CoV-2 antibodies. Results showed that positive individuals had high serum concentrations of chemokines, TNF, IFN-gamma and IL-10. The study also found that the neutralizing capacity of antibodies was significantly related to lower serum concentrations of CCL5 and CXCL10.
Article
Microbiology
Silvia Giannattasio-Ferraz, Adriana Ene, Vitor Junio Gomes, Cid Oliveira Queiroz, Laura Maskeri, Andre Penido Oliveira, Catherine Putonti, Edel F. Barbosa-Stancioli
Summary: The study of livestock microbiota is important for animal health and reducing food contamination and emerging pathogens. This study isolated E. coli and Pseudomonas aeruginosa strains from urine of dairy Gyr cattle. Although these strains are usually associated with urinary tract infections and mastitis, all sampled animals were healthy. The strains from bovine urine had genomic similarity to isolates from the gastrointestinal tract of cattle, while the P. aeruginosa strains were more similar to human isolates, suggesting niche adaptation. Further research is needed to determine if these bacteria are resident members of the bovine urinary microbiota and possible sources of emerging pathogens in humans.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Public, Environmental & Occupational Health
Gabriela de Melo Franco, Anderson Santos da Rocha, Laura Jorge Cox, Danielle Soares de Oliveira Daian e Silva, Debora Marques da Silveira e Santos, Marina Lobato Martins, Luis Claudio Romanelli, Ricardo Ishak, Antonio C. R. Vallinoto, Maria Rosa Q. Bomfim, Adele Caterino-de-Araujo, Jordana G. A. Coelho-dos-Reis, Flavio Guimaraes da Fonseca, Edel Figueiredo Barbosa-Stancioli
Summary: A multi-epitope protein was developed for the serological screening of HTLV-1/2 infection. The test showed high specificity and sensitivity in Brazilian samples, demonstrating its potential for accurate detection of HTLV-1/2 infection. This study is important for supporting public policies to reduce HTLV-1/2 transmission in Brazil.
FRONTIERS IN PUBLIC HEALTH
(2022)
Article
Biotechnology & Applied Microbiology
Andressa Coelho Ferreira, Maria Rosa Quaresma Bomfim, Carlos Henrique de Barros da Costa Sobrinho, Daniela Talissa Lobo Boaz, Railane Da Silva Lira, Valeria Costa Fontes, Mariana Oliveira Arruda, Patricia Maria Wiziack Zago, Carlos Alberto Alves Dias Filho, Carlos Jose Moraes Dias, Marilene Oliveira da Rocha Borges, Rachel Melo Ribeiro, Cicero Wellington Brito Bezerra, Rosiane Silva Penha
Summary: This study aims to produce, characterize, and assess the antimicrobial activity and cytotoxicity of polymer blends based on chitosan and fish collagen. The results showed that these polymer blends have good biocompatibility and antimicrobial activity, making them suitable for biomedical applications.
Article
Immunology
Luis A. F. Andrade, Alice F. Versiani, Edel F. Barbosa-Stancioli, Jenner K. P. dos Reis, Jordana Grazziela A. C. dos Reis, Flavio G. da Fonseca
Summary: The feline immunodeficiency virus (FIV) is a global retrovirus that affects both domestic and wild cats, and can lead to death. Currently, there is only one effective vaccine for certain FIV subtypes, while another common subtype is not preventable by vaccination. Researchers have developed a new vaccine prototype and conducted preclinical tests in mice, showing potential for further development and use in cats.
Article
Immunology
Agata Lopes-Ribeiro, Franklin Pereira Araujo, Patricia de Melo Oliveira, Lorena de Almeida Teixeira, Geovane Marques Ferreira, Alice Aparecida Lourenco, Laura Cardoso Correa Dias, Caio Wilker Teixeira, Henrique Morais Retes, Elisson Nogueira Lopes, Alice Freitas Versiani, Edel Figueiredo Barbosa-Stancioli, Flavio Guimaraes da Fonseca, Olindo Assis Martins-Filho, Moriya Tsuji, Vanessa Peruhype-Magalhaes, Jordana Grazziela Alves Coelho-dos-Reis
Summary: This study aimed to identify MHC-I-restricted peptide signatures for arboviruses using in silico and in vitro peptide microarray tools. The results showed that there were few overlapping immunogenic peptides between different arboviruses, with DENV-derived peptides showing the highest reactivity with HLA-A*02:01. Additionally, non-structural proteins were identified as hotspots for HLA-A*02:01-reactive peptides.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Thaiza Aline Pereira-Santos, Anderson Santos da Rocha, Agata Lopes-Ribeiro, Laura Cardoso Correa-Dias, Patricia Melo-Oliveira, Erik Vinicius de Sousa Reis, Flavio Guimaraes da Fonseca, Edel Figueiredo Barbosa-Stancioli, Moriya Tsuji, Jordana Grazziela Alves Coelho-dos-Reis
Summary: This study aimed to identify immunodominant regions of HTLV-1 Tax using peptide microarray analysis. Results showed dose-dependent reactivity of HLA-A*02:01 in all regions, with specific hotspots. In silico analysis revealed IFN-gamma-inducing peptides in the N-terminal portion and IL-4 inducers in the C-terminal portion. This research provides insight for designing immunomodulatory strategies for HTLV-1 chronic diseases.
Article
Medicine, Research & Experimental
Daniel Wagner de Castro Lima Santos, Yankee Costa Magalhaes Diniz, Raimunda Ribeiro da Silva, Sirlei Garcia Marques, Maria Rosa Quaresma Bomfim, Conceicao de Maria Pedrozo e Silva de Azevedo
Summary: This article presents a case of extensive human cutaneous protothecosis in northeastern Brazil. The diagnosis was based on histopathological examination and culturing of skin samples, followed by identification using mass spectrometry. Treatment with itraconazole was unsuccessful, but liposomal amphotericin followed by voriconazole led to clinical cure. Protothecosis should be considered as a diagnostic possibility in cases with skin lesions after trauma, especially when the evolution is atypical.
MEDICAL MYCOLOGY CASE REPORTS
(2023)
Article
Virology
Thiago Lima Leao, Karine Lima Lourenco, Cid de Oliveira Queiroz, angela Vieira Serufo, Aristobolo Mendes da Silva, Edel F. Barbosa-Stancioli, Flavio Guimaraes da Fonseca
Summary: In this study, it was found that the vaccinia virus strains WR and MVA modulate the UPR pathway to enhance their replication. They activate ATF6α signaling to trigger the expression of endoplasmic reticulum chaperones while inhibiting IRE1α-XBP1 activation.
Article
Microbiology
Artur Serra Neto, Sirlei G. Marques, Maria Rosa Q. Bomfim, Silvio G. Monteiro, Rosangela C. de Souza, Rodolfo A. Nunes
Summary: Hand antisepsis of medical personnel is crucial in patient care as it prevents the spread of pathogenic microorganisms during surgical procedures. This study examined the prevalence of microorganisms on the hands of 131 surgeons before surgery in a university hospital. The results showed a high frequency of microorganisms before handwashing, but after surgical antisepsis, the rate dropped significantly. Gram-positive species, particularly Staphylococcus spp. and Micrococcus luteus, were the most detected, followed by gram-negative species such as Stenotrophomonas maltophilia and Acinetobacter baumanii.
Article
Parasitology
Adele Caterino-de-Araujo, Edel Figueiredo Barbosa-Stancioli, Jose Boullosa Alonso Neto, Mayra Goncalves Aragon, Bernardo Galvao-Castro, Ricardo Ishak, Carolina Rosadas
Summary: This study analyzed and reviewed the laboratory diagnosis of human T-lymphotropic virus 1 and 2 (HTLV-1/2) infections in Brazil, including diagnostic serological and molecular assays, causes of false results, and diagnostic flowcharts. The results support the implementation of public policies to reduce HTLV-1/2 transmission.
REVISTA DA SOCIEDADE BRASILEIRA DE MEDICINA TROPICAL
(2021)
Article
Parasitology
Marina Lobato Martins, Maria Clara Fernandes Da Silva-Malta, Argus Leao Araujo, Fabiola Araujo Goncalves, Maiara de Lourdes Botelho, Isabelle Rocha de Oliveira, Luciana de Souza Madeira Ferreira Boy, Helinse Medeiros Moreira, Edel Figueiredo Barbosa-Stancioli, Maisa Aparecida Ribeiro, Daniel Goncalves Chaves
Summary: The study found that patients with severe COVID-19 and asymptomatic infected individuals both exhibited inflammatory characteristics, with high levels of inflammatory mediators. In contrast, the non-infected group showed lower levels of inflammatory response.
REVISTA DA SOCIEDADE BRASILEIRA DE MEDICINA TROPICAL
(2022)
Article
Veterinary Sciences
L. Alibrandi, R. Tognetti, O. Domenech, M. Croce, M. Giuntoli, G. Grosso, T. Vezzosi
Summary: This study assessed the feasibility and diagnostic reliability of a new smartphone-based ECG device in dogs, and found no significant differences compared to a traditional ECG device. The results suggest that the smartphone-based device is clinically reliable for assessing heart rate and rhythm in dogs.
VETERINARY JOURNAL
(2024)
