期刊
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
卷 155, 期 3, 页码 211-217出版社
ELSEVIER
DOI: 10.1016/j.vetimm.2013.06.013
关键词
Canine; NK cells; Interleukins
资金
- National Institutes of Health of the United States Public Health Service [P30 CA77598]
- University of Minnesota Companion Animal Grant
- University of Minnesota Animal Cancer Care and Research Program/Comparative Oncology Research Fund
NM cells are non-T, non-B lymphocytes that kill target cells without previous activation. The immunophenotype and function of these cells in humans and mice are well defined, but canine NM cells remain incompletely characterized. Our objectives were to isolate and culture canine peripheral blood NM cells, and to define their immunophenotype and killing capability. PBMC were obtained from healthy dogs and T cells were depleted by immunomagnetic separation. The residual cells were cultured in media supplemented with IL-2, IL-15 or both, or with mouse embryonic liver (EL) feeder cells. Non-T, non-B lymphocytes survived and expanded in these cultures. IL-2 was necessary and sufficient for survival; the addition of IL-15 was necessary for expansion, but IL-15 alone did not support survival. Culture with EL cells and IL-2 also fostered survival and expansion. The non-T, non-B lymphocytes uniformly expressed CD45, MHC I, and showed significant cytotoxic activity against CTAC targets. Expression of MHC II, CD11/18 was restricted to subsets of these cells. The data show that cells meeting the criteria for NM cells in other species, i.e., non-T, non-B lymphocytes with cytotoxic activity, can be expanded from canine PBMC by T-cell depletion and culture with cytokines or feeder cells. Published by Elsevier B.V.
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