期刊
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
卷 133, 期 2-4, 页码 133-143出版社
ELSEVIER
DOI: 10.1016/j.vetimm.2009.07.014
关键词
LEAP-2; Antimicrobial peptide; Expression; Liver; Grass carp
资金
- Ministry of Agriculture of China
- Science and Technology Commission of Shanghai Municipal Government [06DJ14003]
- Shanghai Leading Academic Discipline Project [Y1101]
Liver-expressed antimicrobial peptide 2 (LEAP-2) is a novel antimicrobial peptide (AMP) recently found in vertebrates, and exhibits distinct amino acid sequence, secondary structure and expression pattern from other peptides. In this study, the LEAP-2 gene and its full-length cDNA were cloned from grass carp. Grass carp LEAP-2 gene consists of two introns and three exons. The translated product contains 92 amino acids, including a 26 amino acids signal peptide and a mature peptide of 41 amino acids. Grass carp LEAP-2 gene was expressed in a wide range of tissues except blood, with the highest level of transcripts found in liver. Upon induction by Aeromonas hydrophila, its expression was significantly up-regulated in liver, gill, skin, muscle, spleen, blood, head kidney, heart and intestine, but down-regulated in trunk kidney and brain. The transcript level was high in embryos at the 16-cell stage but declined gradually afterwards, suggesting that LEAP-2 transcripts in early embryos might be maternal. Mature peptides obtained by in vitro expression displayed selective antimicrobial activities. These results together further our understanding of the physiological function of LEAP-2 in vertebrates. (C) 2009 Elsevier B.V. All rights reserved.
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