期刊
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
卷 137, 期 1-2, 页码 161-165出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetimm.2010.04.017
关键词
Induced T regulatory cell; TGF; IL-10; Suppression assay
资金
- OARDC
- George and Edna Jaap fund
In mammals, induced T regulatory cells (iTregs) are produced by treating CD4(+)CD25(-) cells in vitro with TGF-beta in the presence of anti-CD3 and IL-2 Chicken CD4(+)CD25(-) cells were treated in vitro with TGF-beta, anti-CD3, anti-CD28 and IL-2 and analyzed for suppressive properties, IL-10 and IL-2 mRNA amounts. TGF-beta treatment decreased (P < 0.01) CD4(+)CD25(-) cell proliferation by 45% at 5d of cell culture with no increase in dead cell population CD4(+)CD25(-) cells treated with 5 ng TGF-beta for 5 d had 130-fold (P < 0.01) more IL-10 and 104-fold (P < 0.01) less IL-2 mRNA content than the 0 ng TGF-beta treated groups. CD4(+)CD25(-) cells treated with 5 ng TGF-beta for 5 d suppressed (P < 0.01) naive cell proliferation compared to the 0 ng TGF-beta treated groups The results suggest the presence of iTreg-like cells in avian species Published by Elsevier B V
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