Review
Biochemistry & Molecular Biology
Sarah S. Wang, Jihao Xu, Keely Y. Ji, Chang-Il Hwang
Summary: Pancreatic cancer, particularly pancreatic ductal adenocarcinoma (PDA), is a significant cause of cancer-related deaths in the United States. While genetic mutations driving PDA initiation and progression have been identified, the mechanisms underlying PDA metastasis remain elusive. It is suggested that epigenetic fluctuations may play a critical role in driving PDA metastasis.
Review
Gastroenterology & Hepatology
Laura D. Wood, Marcia Irene Canto, Elizabeth M. Jaffee, Diane M. Simeone
Summary: This review discusses the pathogenesis, early detection methods, and treatment considerations of pancreatic ductal adenocarcinoma (PDAC). Recent advances in understanding PDAC have led to significant improvements in detection and treatment, but coordinated efforts are needed to maximize clinical impact for patients and overall survival.
Review
Oncology
Yuhao Zhao, Mao Yang, Shijia Wang, Sk Jahir Abbas, Junzhe Zhang, Yongsheng Li, Rong Shao, Yingbin Liu
Summary: This review focuses on the mechanistic insights of DNA, histone, and RNA methylation in regulating the progression of pancreatic cancer. The roles of methylation regulators in modulating gene expression associated with cell proliferation, invasion, and apoptosis are discussed. Recent clinical trials on methylation drug targeting are also explored. Understanding the novel regulatory mechanisms of methylation modification may offer alternative opportunities to improve therapeutic efficacy in combating this devastating disease.
FRONTIERS IN ONCOLOGY
(2022)
Review
Pharmacology & Pharmacy
Chaithanya Ganji, Batoul Farran
Summary: This review focuses on dysregulated genes associated with epigenetic mechanisms in pancreatic cancer progression and resistance, as well as current clinical trials for epigenetic drugs. Combining epigenetic drugs with targeted therapies might represent a promising approach for treating pancreatic cancer.
DRUG DISCOVERY TODAY
(2022)
Review
Biochemistry & Molecular Biology
Simeon Santourlidis, Wolfgang A. Schulz, Marcos J. Arauzo-Bravo, Daniela Gerovska, Pauline Ott, Marcelo L. Bendhack, Mohamed Hassan, Lars Erichsen
Summary: Epigenetic mechanisms play a fundamental role in the initiation and development of melanoma, but research in this area is relatively limited compared to other cancers. This review provides a brief summary of the epigenetic aspects relevant to melanoma pathogenesis and introduces new perspectives from epigenetic research in other cancers that may have potential for melanoma diagnosis and therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Oncology
Maria Victoria Garcia-Ortiz, Pablo Cano-Ramirez, Marta Toledano-Fonseca, Enrique Aranda, Antonio Rodriguez-Ariza
Summary: Pancreatic cancer is a challenging and deadly disease, and there is a need to improve early detection, prognosis, and treatment response. Liquid biopsy and cell-free DNA (cfDNA) methylation analysis have emerged as promising approaches for developing biomarkers in pancreatic cancer research.
BIOMARKER RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Yang Zhao, Christine M. O'Keefe, Kuangwen Hsieh, Leslie Cope, Sonali C. Joyce, Thomas R. Pisanic, James G. Herman, Tza-Huei Wang
Summary: There is a strong interest in developing liquid biopsy assays for detecting cancer-specific alterations in circulating tumor DNA obtained from noninvasive blood draws. This paper describes the development and validation of a multiplex digital methylation-specific PCR platform for the detection of non-small cell lung cancer (NSCLC) in liquid biopsy samples. The platform demonstrates high analytical sensitivity and superior clinical performance compared to traditional MSP assays.
Article
Biotechnology & Applied Microbiology
Vadim Fedyuk, Nir Erez, Noa Furth, Olga Beresh, Ekaterina Andreishcheva, Abhijeet Shinde, Daniel Jones, Barak Bar Zakai, Yael Mavor, Tamar Peretz, Ayala Hubert, Jonathan E. Cohen, Azzam Salah, Mark Temper, Albert Grinshpun, Myriam Maoz, Aviad Zick, Guy Ron, Efrat Shema
Summary: This study developed a single-molecule imaging technique that can accurately and sensitively detect colorectal cancer in cell-free nucleosomes. By analyzing the epigenetics, DNA methylation, and cancer-specific protein biomarkers of plasma-isolated nucleosomes, the system allows for high-resolution detection of histone modifications and provides quantitative data on plasma proteins. The analysis showed high accuracy and sensitivity in detecting cancer, even at early stages, and could determine the tissue origin of tumors.
NATURE BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Groves Dixon, Mikhail Matz
Summary: In this study, three different methods for measuring DNA methylation were evaluated, with MBD-seq and mdRAD identified as reliable and cost-effective alternatives to WGBS. The lower sequencing effort required for mdRAD to produce comparable methylation estimates makes it particularly useful for ecological epigenetics research. The three assays showed similar performance in measuring absolute methylation levels for gene bodies, exons, and 1 Kb windows, with mdRAD standing out as a promising method for ecological epigenetics.
MOLECULAR ECOLOGY RESOURCES
(2021)
Review
Genetics & Heredity
Sonam Tulsyan, Mehreen Aftab, Sandeep Sisodiya, Asiya Khan, Atul Chikara, Pranay Tanwar, Showket Hussain
Summary: The global cancer cases and mortality rates are increasing, and efficient biomarkers are needed for accurate screening and diagnosis. Alterations in epigenetic marks may serve as potential biomarkers for early cancer detection. This review discusses the key epigenetic mechanisms and their deregulation in cancer etiology, exploring the future prospects of cancer precision medicine.
FRONTIERS IN GENETICS
(2022)
Article
Genetics & Heredity
Jennifer Kerkhof, Gabriella Maria Squeo, Haley McConkey, Michael A. Levy, Maria Rosaria Piemontese, Marco Castori, Maria Accadia, Elisa Biamino, Matteo Della Monica, Marilena Carmela Di Giacomo, Cristina Gervasini, Silvia Maitz, Daniela Melis, Donatella Milani, Maria Piccione, Paolo Prontera, Angelo Selicorni, Bekim Sadikovic, Giuseppe Merla
Summary: This study analyzed the methylation profiles of individuals with chromatinopathies and identified unique genome-wide DNA methylation profiles, which were matched and confirmed with known episignatures. Additionally, uncertain variants were reclassified as likely pathogenic, and specific chromatinopathy disorders were identified in some sequencing negative cases. Therefore, DNA methylation analysis plays an important role in diagnosing chromatinopathies.
GENETICS IN MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Eshaan Patnaik, Chikezie Madu, Yi Lu
Summary: Epigenetics plays a crucial role in gene regulation and tumor development. DNA methylation inhibitors and histone deacetylase inhibitors can restore normal gene expression and be effective against cancer. Understanding epigenetic modifications and utilizing inhibitors offer new possibilities for cancer research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Ines Faleiro, Vania Palma Roberto, Secil Demirkol Canli, Nicolas A. Fraunhoffer, Juan Iovanna, Ali Osmay Gure, Wolfgang Link, Pedro Castelo-Branco
Summary: This study investigated the potential of DNA methylation levels of PI3K/AKT-related genes in diagnosing and predicting the clinical outcomes of pancreatic cancer. It identified several genes as potential diagnostic tools and independent prognostic indicators, providing evidence for the use of epigenetic alterations as biomarkers for disease screening and management, as well as highlighting possible therapeutic targets.
Review
Biochemistry & Molecular Biology
Kangchun Wang, Xin Wang, Qi Pan, Bei Zhao
Summary: Liquid biopsy is a noninvasive detection method that shows promising prospects in the diagnosis, treatment monitoring, and prognosis assessment of pancreatic cancer.
Article
Oncology
Alexis Overs, Mylene Flammang, Eric Hervouet, Laurent Bermont, Jean-Luc Pretet, Borg Christophe, Zohair Selmani
Summary: The study found that hypermethylation of NPY and WIF1 are consistent specific biomarkers of CRCs, regardless of histological characteristics, tumor stages or mutation status. The hypermethylation of NPY or WIF1 in liquid biopsy showed high sensitivity and specificity for the detection of CRCs.
Article
Biochemistry & Molecular Biology
Rongjun Chen, Sariah Khormaee, Mark E. Eccleston, Nigel K. H. Slater
Article
Engineering, Biomedical
Rongjun Chen, Zhilian Yue, Mark E. Eccleston, Nigel K. H. Slater
Article
Engineering, Biomedical
Rongjun Chen, Sariah Khormaee, Mark E. Eccleston, Nigel K. H. Slater
Article
Chemistry, Physical
Rongjun Chen, Mark E. Eccleston, Zhilian Yue, Nigel K. H. Slater
JOURNAL OF MATERIALS CHEMISTRY
(2009)
Article
Nanoscience & Nanotechnology
Xiaowen Dai, Zhilian Yue, Mark E. Eccleston, Johannes Swartling, Nigel K. H. Slater, Clemens F. Kaminski
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2008)
Article
Pharmacology & Pharmacy
Mark Eccleston, George Morris
PERSONALIZED MEDICINE
(2008)
Review
Genetics & Heredity
Ning Kang, Mark Eccleston, Pier-Luc Clermont, Maryam Latarani, David Kingsley Male, Yuzhuo Wang, Francesco Crea
Article
Oncology
Jeroen G. H. P. Verhoeven, Carla C. Baan, Annemiek M. A. Peeters, Marian C. Clahsen-van Groningen, Daan Nieboer, Marielle Herzog, Marc Eccleston, Dennis A. Hesselink, Karin Boer
Summary: In this pilot study, total circulating cell-free nucleosomes (H3) concentrations are increased during acute kidney transplant rejection. The high negative predictive value suggests that when a patient experiences loss of renal transplant function and total CCFN (H3) is not increased, causes other than acute rejection should be considered. Clinical implementation of total CCFN (H3) measurement may help avoid unnecessary and potentially harmful kidney transplant biopsies.
CLINICAL EPIGENETICS
(2021)
Article
Biochemistry & Molecular Biology
Etienne Cavalier, Julien Guiot, Katharina Lechner, Alexander Dutsch, Mark Eccleston, Marielle Herzog, Thomas Bygott, Adrian Schomburg, Theresa Kelly, Stefan Holdenrieder
Summary: The study found elevated levels of nucleosomes in the plasma of COVID-19 patients, especially in those with severe disease. Increased citrullinated nucleosomes levels indicate neutrophil activation in severely affected individuals. Furthermore, nucleosome levels correlate with disease severity and may serve as a guiding biomarker for treatment.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Genetics & Heredity
Francesca Salani, Maryam Latarani, Andrea Casadei-Gardini, Priyadarsini Gangadharannambiar, Lorenzo Fornaro, Caterina Vivaldi, Irene Pecora, Valentina Massa, Giorgia Marisi, Matteo Canale, Paola Ulivi, Mario Scartozzi, Mark Eccleston, Gianluca Masi, Francesco Crea
Summary: The circulating H3K27me3/H3K36me3 ratio acts as a predictive biomarker for sorafenib treatment outcomes in patients with advanced hepatocellular carcinoma.
Article
Multidisciplinary Sciences
Priscilla Van den Ackerveken, Alison Lobbens, Dorian Pamart, Aristotelis Kotronoulas, Guillaume Rommelaere, Mark Eccleston, Marielle Herzog
Summary: This study found that the levels of H3.1-nucleosomes are elevated in the plasma samples of patients with non-Hodgkin lymphoma (NHL) compared to healthy donors. The study also identified eight histone post-translational modifications (PTMs) that were present at higher levels in NHL patients' plasma. These findings suggest that H3.1-nucleosomes and histone PTMs may serve as useful biomarkers for diagnosing and monitoring non-Hodgkin lymphoma.
SCIENTIFIC REPORTS
(2023)
Article
Engineering, Chemical
Mark Eccleston
Article
Polymer Science
XW Dai, ME Eccleston, ZL Yue, NKH Slater, CF Kaminski
Article
Chemistry, Multidisciplinary
RJ Chen, ZL Yue, ME Eccleston, S Williams, NKH Slater
JOURNAL OF CONTROLLED RELEASE
(2005)