Article
Cell & Tissue Engineering
Xiao Bai, Zaiwen Qi, Mingzhen Zhu, Zhuangzhuang Lu, Xin Zhao, Lining Zhang, Guangmin Song
Summary: The study found that lncRNA MIR155HG can promote the survival and migration of MSCs and reduce the apoptosis of HUVECs through exosome delivery. The combined treatment with MSCs and exosomes modified with MIR155HG can effectively attenuate intimal hyperplasia of vein graft.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Cardiac & Cardiovascular Systems
Dengshen Zhang, Yiran Cao, Daxing Liu, Jian Zhang, Yingqiang Guo
Summary: This article reviews the key pathophysiological mechanism of vein graft restenosis, which is the phenotypic transformation of venous SMCs from differentiated to dedifferentiated phenotypes, and the role of miRNAs in this process.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Review
Cell Biology
Shameem S. Ladak, Liam W. McQueen, Georgia R. Layton, Hardeep Aujla, Adewale Adebayo, Mustafa Zakkar
Summary: Endothelial cells play a crucial role in maintaining vascular health and preventing vascular diseases. Dysfunction of the endothelium is a key driver for vein graft disease, resulting in poor long-term patency rates after coronary artery bypass graft surgery.
Article
Biochemistry & Molecular Biology
Liam W. McQueen, Shameem S. Ladak, Mustafa Zakkar
Summary: The long saphenous vein is commonly used in cardiac surgery to bypass occluded coronary arteries. However, its use is complicated by late stenosis and occlusion due to intimal hyperplasia. This review examines the multifactorial inflammatory process of intimal hyperplasia and the impact of acute shear stress changes on pro-inflammatory mediators.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Justine R. Stehn, Scott R. Floyd, Erik W. Wilker, H. Christian Reinhardt, Scott M. Clarke, Qiuying Huang, Roberto D. Polakiewicz, Nahum Sonenberg, Yi Wen Kong, Michael B. Yaffe
Summary: 14-3-3 proteins play critical roles in regulating multiple cellular processes and their phosphorylation on specific residues is required for binding to protein kinases. To better understand this interaction, a biochemical approach was used to identify PABPC1 as a substrate for Chk1 and MK2 kinases, with Ser-470 being the critical binding site for 14-3-3. Loss of PABPC1 binding to 14-3-3 resulted in increased cell proliferation and decreased cell death in response to UV-induced DNA damage.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Immunology
Lucia Suarez-Lopez, Yi Wen Kong, Ganapathy Sriram, Jesse C. Patterson, Samantha Rosenberg, Sandra Morandell, Kevin M. Haigis, Michael B. Yaffe
Summary: The activity of MK2 in macrophages is essential for tumor angiogenesis during inflammation-induced cancer progression, by regulating various pro-angiogenesis factors, including CXCL-12/SDF-1 secreted by tumor associated-macrophages.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Pharmacology & Pharmacy
Tamara Melnik, Olivier Jordan, Jean-Marc Corpataux, Florence Delie, Francois Saucy
Summary: This article reviews the development pathways of intimal hyperplasia (IH) and discusses different drugs and drug combinations that interfere with these pathways. Local application of drugs and drug combinations may provide an effective and safe approach for preventing IH.
PHARMACOLOGY & THERAPEUTICS
(2022)
Article
Multidisciplinary Sciences
T. Girao-Silva, M. H. Fonseca-Alaniz, J. C. Ribeiro-Silva, J. Lee, N. P. Patil, L. A. Dallan, A. B. Baker, M. C. Harmsen, J. E. Krieger, A. A. Miyakawa
Summary: High stretch conditions induce dysfunction in human saphenous vein endothelial cells, characterized by reduced NO bioavailability, increased inflammatory adhesion molecule expression, and elevated cell adhesion. Additionally, high stretch leads to actin remodeling and increased oxidative stress, which can be alleviated by pre-treatment with antioxidants.
SCIENTIFIC REPORTS
(2021)
Article
Hematology
Vijay Kondreddy, Shiva Keshava, Kaushik Das, Jhansi Magisetty, L. Vijaya Mohan Rao, Usha R. Pendurthi
Summary: This study reveals the important role of the Gab2-MALT1 axis in thromboinflammation. Targeting this axis may be an effective strategy to treat DVT without inducing bleeding complications.
Article
Biochemistry & Molecular Biology
Debao Li, Lisheng Qiu, Haifa Hong, Hao Chen, Peibin Zhao, Yingying Xiao, Hao Zhang, Qi Sun, Lincai Ye
Summary: A neonatal rat model of pulmonary vein stenosis (PVS) was successfully established, providing insights into the mechanisms and potential treatments for PVS.
CELL AND BIOSCIENCE
(2023)
Article
Cardiac & Cardiovascular Systems
Davide Maselli, Gloria Garoffolo, Giada Andrea Cassanmagnago, Rosa Vono, Matthijs S. Ruiter, Anita C. Thomas, Paolo Madeddu, Maurizio Pesce, Gaia Spinetti
Summary: Mechanical stress promotes molecular phenotypic switching of saphenous vein progenitors and increases their responsiveness to extracellular environment alterations.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Review
Cardiac & Cardiovascular Systems
Oluwatomini Fashina, Riccardo G. Abbasciano, Liam W. McQueen, Shameem Ladak, Sarah J. George, Sadeeh Suleiman, Prakash P. Punjabi, Gianni D. Angelini, Mustafa Zakkar
Summary: Coronary artery bypass grafting is the preferred treatment for patients with significant coronary disease, but vein graft patency remains a challenge. Research models, especially large animal models, play a vital role in understanding the pathophysiology of intimal hyperplasia.
Article
Cell Biology
Xue-Hua Du, Shao-Bo Ke, Xin-Yi Liang, Jie Gao, Xiao-Xiao Xie, Lin-Zhi Qi, Xue-Yi Liu, Guo-Yuan Xu, Xiao-Dong Zhang, Run-Lei Du, Shang-Ze Li
Summary: Our study demonstrates that USP14 functions as a deubiquitinase that interacts and stabilizes JNK, promoting MAPK/JNK signaling and colorectal carcinogenesis. Increased expression of USP14 is associated with elevated levels of JNK protein and downstream gene expression in colorectal cancer patients. Inhibition of USP14 reduces cancer cell proliferation and tumorigenesis by downregulating the activation of the MAPK/JNK pathway.
CELL DEATH & DISEASE
(2023)
Review
Biochemistry & Molecular Biology
Aziz M. Rangwala, Victoria R. Mingione, George Georghiou, Markus A. Seeliger
Summary: Phosphorylation plays a vital role in regulating biological processes, and protein kinases have been extensively studied for their involvement in human health and disease. Some kinases possess additional catalytic functions in addition to phosphotransferase activity, while others have lost their catalytic activity completely. In this study, we analyzed the UniProtKB database for bifunctional protein kinases and focused on those crucial for bacterial and human cellular homeostasis. These kinases have diverse functional roles in environmental sensing, metabolic regulation, immune-host defense, and cell cycle control. This article explores their dual catalytic activities and their contributions to disease pathogenesis.
Article
Biochemistry & Molecular Biology
Jung-Hwan Kim, Eui Hwa Jang, Ji-Yeon Ryu, Jiyong Lee, Jae Ho Kim, Wonhyoung Ryu, Young-Nam Youn
Summary: We investigated the effectiveness of a sirolimus-embedded silk microneedle wrap as an external vascular device for drug delivery, inhibition of neointimal hyperplasia, and vascular remodeling. In a vein graft model using dogs, the sirolimus-embedded silk MN wrap successfully delivered the drug to the intimal layer and prevented vein graft dilatation. It also inhibited neointimal hyperplasia, resulting in decreased collagen density in the intima layer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Hematology
Haidi Hu, Shin-Rong Lee, Hualong Bai, Jianming Guo, Takuya Hashimoto, Toshihiko Isaji, Xiangjiang Guo, Tun Wang, Katharine Wolf, Shirley Liu, Shun Ono, Bogdan Yatsula, Alan Dardik
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2020)
Article
Surgery
Kirstyn E. Brownson, Ramak Khosravi, Shin-Rong Lee, Kimberly Goldstein, Toshihiko Isaji, Shun Ono, Clinton D. Protack, Jay D. Humphrey, Alan Dardik
JOURNAL OF SURGICAL RESEARCH
(2020)
Article
Surgery
Tun Wang, Jia Liu, Haiyang Liu, Shin-Rong Lee, Luis Gonzalez, Jolanta Gorecka, Chang Shu, Alan Dardik
Summary: This study reveals that activation of EphrinB2 signaling during AVF maturation is associated with venous remodeling and could be a strategy to improve AVF patency.
JOURNAL OF SURGICAL RESEARCH
(2021)
Article
Hematology
Yutaka Matsubara, Gathe Kiwan, Jia Liu, Luis Gonzalez, John Langford, Mingjie Gao, Xixiang Gao, Ryosuke Taniguchi, Bogdan Yatsula, Tadashi Furuyama, Takuya Matsumoto, Kimihiro Komori, Alan Dardik
Summary: In this study, it was found that T-cells precede macrophages in the remodeling AVF wall, and regulation of macrophage accumulation by T-cells is important for promoting AVF maturation. This research suggests that targeting T-cells during AVF maturation could be a potential strategy to improve AVF maturation.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2021)
Article
Cell Biology
Xixiang Gao, Mingjie Gao, Jolanta Gorecka, John Langford, Jia Liu, Jiesi Luo, Ryosuke Taniguchi, Yutaka Matsubara, Hao Liu, Lianrui Guo, Yongquan Gu, Yibing Qyang, Alan Dardik
Summary: Research suggests that treatment using human iPSC-derived smooth muscle cells in a murine hindlimb ischemia model can improve functional outcomes and blood flow, increase muscle fiber area and capillary density, reduce macrophage numbers, and enhance vascular endothelial growth factor expression in ischemic limbs.
Review
Cardiac & Cardiovascular Systems
Shin Mei Chan, Gabe Weininger, John Langford, Daniel Jane-Wit, Alan Dardik
Summary: Vascular disorders present differently in women and men, with sex differences in AVF maturation and utilization. Inflammation plays a significant role in AVF maturation, suggesting potential for sex-specific vascular therapies to improve success rates.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Hematology
Yutaka Matsubara, Luis Gonzalez, Gathe Kiwan, Jia Liu, John Langford, Mingjie Gao, Xixiang Gao, Ryosuke Taniguchi, Bogdan Yatsula, Tadashi Furuyama, Takuya Matsumoto, Kimihiro Komori, Masaki Mori, Alan Dardik
Summary: Inhibition of PD-L1 during AVF maturation leads to changes in T cell populations and macrophages, resulting in reduced vascular wall thickening and AVF patency. The effects of anti-PD-L1 antibody on venous remodeling are T cell-dependent, suggesting PD-L1 as a potential therapeutic target for improving AVF maturation.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2021)
Article
Hematology
Ryosuke Taniguchi, Yuichi Ohashi, Jung Seok Lee, Haidi Hu, Luis Gonzalez, Weichang Zhang, John Langford, Yutaka Matsubara, Bogdan Yatsula, George Tellides, Tarek M. Fahmy, Katsuyuki Hoshina, Alan Dardik
Summary: Endothelial cell-targeted TGF-beta inhibition may be a translational strategy to improve AVF patency.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2022)
Article
Peripheral Vascular Disease
Jia Liu, Dingyao Zhang, Anand Brahmandam, Yutaka Matsubara, Mingjie Gao, Jingru Tian, Bing Liu, Chang Shu, Alan Dardik
Summary: This study reveals the activation of inflammatory and immune responses during arteriovenous fistula (AVF) maturation. The regulation of genes associated with AVF maturation differs between male and diabetic patients. Diabetes may impair AVF maturation by altering inflammatory and immune responses.
JOURNAL OF VASCULAR ACCESS
(2022)
Editorial Material
Surgery
Alan Dardik
JOURNAL OF VASCULAR SURGERY
(2022)
Article
Biochemistry & Molecular Biology
Mingjie Gao, Xixiang Gao, Ryosuke Taniguchi, Anand Brahmandam, Yutaka Matsubara, Jia Liu, Hao Liu, Weichang Zhang, Alan Dardik
Summary: During arterial remodeling, female rats exhibited decreased neointimal thickness, which was associated with alterations in arterial identity dependent on intact sex hormones. The alteration of arterial identity may contribute to the sex differences in neointimal proliferation after arterial injury.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Surgery
Mytien Nguyen, Luis Gonzalez, Ashley Newman, Ashley Cannon, Shenika A. Zarebski, Sarwat I. Chaudhry, Bohdan Pomahac, Dowin Boatright, Alan Dardik
Summary: Research indicates that surgeon-scientists are underrepresented in NIH funding, emphasizing the need for more support and funding.
Article
Medicine, General & Internal
Mytien Nguyen, Luis Gonzalez, Sarwat I. Chaudhry, Nita Ahuja, Bohdan Pomahac, Ashley Newman, Ashley Cannon, Shenika A. Zarebski, Alan Dardik, Dowin Boatright
Summary: This study examines the distribution of biomedical research funding by the NIH among male and female surgeon-scientists. The findings show that although women surgeon-scientists have achieved success in receiving NIH funding, they are still significantly underrepresented as super principal investigators. This highlights the need for further investigation and efforts to promote funding distribution among women surgeon-scientists.
Article
Cardiac & Cardiovascular Systems
Keyuree Satam, Yuichi Ohashi, Carly Thaxton, Luis Gonzalez, Ocean Setia, Hualong Bai, Yukihiko Aoyagi, Yangzhouyun Xie, Weichang Zhang, Bogdan Yatsula, Kathleen A. Martin, Yujun Cai, Alan Dardik
Summary: In this mouse model study, it was found that arteriovenous fistulae (AVF) fail to mature more frequently in female patients compared with male patients. Sex hormones were identified as potential mediators of these sex differences in AVF maturation, with testosterone associated with reduced shear stress and estrogen associated with increased immune cell recruitment.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2023)
Article
Surgery
Keyuree Satam, Ocean Setia, Miranda S. Moore, Eric Schneider, Cassius Iyad Ochoa Chaar, Alan Dardik
Summary: Arteriovenous fistulae mature less frequently in women than in men, possibly due to both anatomical and physiological differences in arterial inflow.
ANNALS OF VASCULAR SURGERY
(2023)