期刊
VACCINE
卷 32, 期 23, 页码 2682-2687出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2014.03.079
关键词
Clostridium perfringens; Epsilon toxin pore-forming toxin; Enterotoxemia; Recombinant vaccine
资金
- Wellcome Trust [WT089618MA]
- European Union Marie Curie Network [237942]
Epsilon toxin (Etx) is a beta-pore-forming toxin produced by Clostridium perfringens toxinotypes B and D and plays a key role in the pathogenesis of enterotoxemia, a severe, often fatal disease of ruminants that causes significant economic losses to the farming industry worldwide. This study aimed to determine the potential of a site-directed mutant of Etx (Y30A-Y196A) to be exploited as a recombinant vaccine against enterotoxemia. Replacement of Y30 and Y196 with alanine generated a stable variant of Etx with significantly reduced cell binding and cytotoxic activities in MDCK.2 cells relative to wild type toxin (>430-fold increase in CT50) and Y30A-Y196A was inactive in mice after intraperitoneal administration of trypsin activated toxin at 1000x the expected LD50 dose of trypsin activated wild type toxin. Moreover, polyclonal antibody raised in rabbits against Y30A-Y196A provided protection against wild type toxin in an in vitro neutralisation assay. These data suggest that Y30A-Y196A mutant could form the basis of an improved recombinant vaccine against enterotoxemia. (C) 2014 The Authors. Published by Elsevier Ltd.
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