Review
Pharmacology & Pharmacy
Neftali Ortega Alarcon, Maddy Jaramillo, Heidi M. Mansour, Bo Sun
Summary: This review summarizes the current progress of therapeutic subunit vaccines for cancer immunotherapy, including the classification of tumor antigens and the bioinformatic approaches for identifying tumor neoantigens. The targeting adjuvants based on pattern recognition receptors (PRRs) and their delivery platforms are extensively discussed, emphasizing the importance of the interplay between multiple adjuvants and their combined delivery in cancer immunotherapy.
Review
Immunology
Melika Moradi, Farzaneh Vahedi, Arian Abbassioun, Arash Ramezanpour Shahi, Mohammad Sholeh, Mortaza Taheri-Anganeh, Zahra Dargahi, Roya Ghanavati, Seyyed Hossein Khatami, Ahmad Movahedpour
Summary: According to the World Health Organization, 10 million people worldwide are infected with tuberculosis (TB), resulting in 1.5 million deaths, with 214,000 individuals co-infected with HIV. The development of an effective TB vaccine is crucial due to the high infection rate. Various methodologies, including the protein subunit vaccine, have been proposed, demonstrating higher protection than Bacillus culture vaccines. The liposomal adjuvant system is a safe and efficient method for TB vaccination, as well as for other intracellular infections and malignancies.
IMMUNITY INFLAMMATION AND DISEASE
(2023)
Review
Biochemistry & Molecular Biology
Nunzianna Doti, Mario Mardirossian, Annamaria Sandomenico, Menotti Ruvo, Andrea Caporale
Summary: Retro-inverso peptides, possessing reversed sequences and chirality compared to parent molecules, show promise as more stable surrogates with increased potential as drugs, especially in specific cases. However, their inability to adopt the 3D structure of parent peptides requires careful evaluation and investigation of their applications on a case-by-case basis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Fisheries
Raja Aadil Hussain Bhat, Dimpal Thakuria, Ritesh Shantilal Tandel, Victoria C. Khangembam, Pragyan Dash, Gayatri Tripathi, Debajit Sarma
Summary: Fisheries and aquaculture industries are crucial for providing food and nutrition to millions of people worldwide. However, the indiscriminate use of antibiotics has resulted in the emergence of antimicrobial-resistant bacteria, posing a significant threat to public health. Researchers suggest that antimicrobial peptides (AMPs) could be an effective alternative to combat antimicrobial resistance in aquaculture.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Ehud Shahar, Ken Emquies, Itai Bloch, Dalia Eliahu, Ran Ben Adiva, Jacob Pitcovski, Itamar Yadid
Summary: This study demonstrates the potential of LPS-free gram-negative bacteria as an important tool for protein expression, with wide applications in research and biotechnology.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biophysics
Emanuelle D. Freitas, Rogerio A. Rataglioli, Josephine Oshodi, Marisa M. Beppu
Summary: The coronavirus pandemic has highlighted the importance of bioactive materials in mitigating viral infections. Bioinspired antimicrobial peptides (AMPs) have emerged as suitable building blocks for antimicrobial coatings due to their versatile design and environmentally friendly features. This review discusses the advances and opportunities in using AMPs to create virucidal coatings, including the fundamental characteristics of peptide structure, advances in coating medical devices, and the role of different materials in designing antiviral coatings.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Immunology
Elham Jafari, Shirin Mahmoodi
Summary: The study designed a potential vaccine against Helicobacter Pylori using immunoinformatics, incorporating selected epitopes and honey bee venom protein as an adjuvant. Experimental results showed high antigenicity and non-allergenic potential of the vaccine.
MICROBIAL PATHOGENESIS
(2021)
Article
Microbiology
Li Yi, Qingying Fan, Yuxin Wang, Chenlong Mao, Jinpeng Li, Manyu Jin, Xiaoling Zhang, Ke Ding, Yang Wang
Summary: The study demonstrated that recombinant PDH protein induced high levels of antibodies in mice, enhancing the immune response and protecting pathological organs from damage. Additionally, PDH antiserum significantly inhibited the growth of S. suis and the formation of biofilm in vitro.
VETERINARY MICROBIOLOGY
(2021)
Article
Engineering, Biomedical
Laura T. Gray, Michal M. Raczy, Priscilla S. Briquez, Tiffany M. Marchell, Aaron T. Alpar, Rachel P. Wallace, Lisa R. Volpatti, Maria Stella Sasso, Shijie Cao, Mindy Nguyen, Aslan Mansurov, Erica Budina, Elyse A. Watkins, Ani Solanki, Nikolaos Mitrousis, Joseph W. Reda, Shann S. Yu, Andrew C. Tremain, Ruyi Wang, Vlad Nicolaescu, Kevin Furlong, Steve Dvorkin, Balaji Manicassamy, Glenn Randall, D. Scott Wilson, Marcin Kwissa, Melody A. Swartz, Jeffrey A. Hubbell
Summary: Spike-p(Man-TLR7) vaccination induces robust antigen-specific cellular and humoral responses in mice, generating high and long-lasting titers of antibodies with neutralizing abilities surpassing convalescent human serum. The vaccine also enhances germinal center B cells, antigen-specific antibody secreting cells, activated T follicular helper cells, and polyfunctional Th1-cytokine producing CD4(+) and CD8(+) T cells.
Article
Immunology
James Baber, Mark Arya, Yuben Moodley, Anna Jaques, Qin Jiang, Kena A. Swanson, David Cooper, Mohan S. Maddur, Jakob Loschko, Alejandra Gurtman, Kathrin U. Jansen, William C. Gruber, Philip R. Dormitzer, Beate Schmoele-Thoma
Summary: The stabilized RSV prefusion F subunit (RSVpreF) vaccine candidate was well tolerated and elicited strong and persistent serum neutralizing responses in adults aged 65-85, with or without adjuvant. Adjuvanted formulations did not show additional enhancement of immune responses.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Immunology
Renato B. Baleeiro, Louisa S. Chard Dunmall, Peng Liu, Shuangshuang Lu, Yuchun Lone, Nicholas R. Lemoine, Yaohe Wang
Summary: RAS mutations can be targeted by immunotherapy as immunogenic peptides derived from these mutations can activate specific CD8+ T cell responses and exhibit cytotoxic effects on tumor cells expressing the mutations.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jose-Luis Velasco-Bolom, Ramon Garduno-Juarez
Summary: Understanding the effect of antimicrobial peptides on biological membranes is important for combating multidrug-resistant bacteria. In this study, molecular dynamics simulations were used to investigate the pore formation by antimicrobial peptide Pin2 on model membranes. The results suggest that Pin2 forms toroidal pores, which are selective for chlorine ions.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Medicine, General & Internal
Lindsey R. Baden, Hana M. El Sahly, Brandon Essink, Karen Kotloff, Sharon Frey, Rick Novak, David Diemert, Stephen A. Spector, Nadine Rouphael, C. Buddy Creech, John McGettigan, Shishir Khetan, Nathan Segall, Joel Solis, Adam Brosz, Carlos Fierro, Howard Schwartz, Kathleen Neuzil, Larry Corey, Peter Gilbert, Holly Janes, Dean Follmann, Mary Marovich, John Mascola, Laura Polakowski, Julie Ledgerwood, Barney S. Graham, Hamilton Bennett, Rolando Pajon, Conor Knightly, Brett Leav, Weiping Deng, Honghong Zhou, Shu Han, Melanie Ivarsson, Jacqueline Miller, Tal Zaks
Summary: The mRNA-1273 vaccine demonstrated 94.1% efficacy in preventing Covid-19 illness, including severe cases. Mild, transient local reactions were the only adverse effects, with low incidence of systemic reactions such as fever, headache, and fatigue.
NEW ENGLAND JOURNAL OF MEDICINE
(2021)
Article
Chemistry, Multidisciplinary
Yu Wen, Ru-Yan Zhang, Jian Wang, Shi-Hao Zhou, Xiao-Qian Peng, Dong Ding, Zhi-Ming Zhang, Hua-Wei Wei, Jun Guo
Summary: Self-adjuvanting protein vaccines with efficient codelivery of adjuvant and antigen have been proven to be highly immunogenic. However, current protein vaccines with built-in adjuvants lack the ability to achieve minor epitope interference and adjuvant multivalency simultaneously. In this study, a new conjugate strategy was developed to construct an effective adjuvant-protein vaccine with adjuvant cluster effect and minimal epitope interference.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Biochemistry & Molecular Biology
Abu Tayab Moin, Gagandeep Singh, Nafisa Ahmed, Syeda Afra Saiara, Vladimir Timofeev, Nairita Ahsan Faruqui, Shama Sharika Ahsan, Afrida Tabassum, Sadman Sakib Nebir, K. M. Salim Andalib, Yusha Araf, Md Asad Ullah, Bishajit Sarkar, Nafisa Nawal Islam, Umme Salma Zohora
Summary: Human cytomegalovirus (HCMV) is a widespread virus that can cause serious neurological damage in newborns and even death in children. Existing treatments have limitations due to side effects and drug resistance. In this study, an immunoinformatics approach was used to design a potential vaccine effective against four virulent strains of HCMV. The vaccine was found to be stable, safe, and effective through various experiments and simulations.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Fisheries
Mayte Gonzalez, Jesus Luis Betancourt, Tania Rodriguez-Ramos, Mario Pablo Estrada, Yamila Carpio, Laida Ramos
AQUACULTURE RESEARCH
(2020)
Article
Fisheries
Janet Velazquez, Geysi Perez, Shawna L. Semple, Tania Rodriguez-Ramos, Patricia Diaz-Rosales, Maria del Camino Ordas, Juana Maria Lugo, Brian Dixon, Carolina Tafalla, Mario Pablo Estrada, Yamila Carpio
FISH & SHELLFISH IMMUNOLOGY
(2020)
Article
Multidisciplinary Sciences
Felipe E. Bravo, Natalie C. Parra, Frank Camacho, Jannel Acosta, Alain Gonzalez, Jorge R. Toledo, Oliberto Sanchez
SCIENTIFIC REPORTS
(2020)
Article
Multidisciplinary Sciences
Jaya Kumari Swain, Yamila Carpio, Lill-Heidi Johansen, Janet Velazquez, Liz Hernandez, Yeny Leal, Ajey Kumar, Mario Pablo Estrada
Article
Fisheries
Janet Velazquez, Alianet Rodriguez, Hasel Aragon, Arlette Haidar, Marcos Gonzalez, Rodolfo Valdes, Hilda Elsa Garay, David Diago Abreu, Yassel Ramos, Ania Cabrales, Antonio Morales, Osmany Gonzalez, Fidel Herrera, Mario Pablo Estrada, Yamila Carpio
Summary: In this study, a monoclonal antibody against tilapia IgM heavy chain was successfully produced and characterized using a peptide-based strategy. The antibody showed specificity and efficacy in studying the humoral immune response in fish species, demonstrating its utility as a molecular tool for fish immunology research.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Fisheries
Alianet Rodriguez, Janet Velazquez, Luis Gonzalez, Tania Rodriguez-Ramos, Brian Dixon, Fidel Herrera Miyares, Antonio Morales, Osmany Gonzalez, Mario Pablo Estrada, Yamila Carpio
Summary: PACAP-38 from African catfish enhances antibacterial activity and modulates gene expression of immune-related molecules, suggesting its potential as an antimicrobial agent in aquaculture.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Fisheries
Fidel Herrera, Janet Velazquez, Juana Maria Lugo, Paola Orellana, Joceline Ruiz, Matias Vega, Alex Romero, Nestor Santos, Gerardo Ramses, Tania Rodriguez-Ramos, Brian Dixon, Mario Pablo Estrada, Patricio Dantagnan, Yamila Carpio
Summary: This study demonstrated that dietary supplementation with synthetic PACAP-38 can improve PUFA content in muscle, increase the number of caliciform cells within intestinal villi, and modulate the transcription of cytokines in head kidney of rainbow trout fingerlings. These findings suggest potential benefits of using small peptides as feed additives to enhance the quality of cultured fish fingerlings.
AQUACULTURE REPORTS
(2021)
Article
Fisheries
Liz Hernandez, Hanlet Camacho, Adriana Nunez-Robainas, Daniel O. Palenzuela, Antonio Morales, Liliana Basabe, Fidel Herrera, Osmany Rodrigo, Adrian Rodriguez-Gabilondo, Janet Velazquez, Soraya Piloto, Mario P. Estrada, Rebeca Martinez
Summary: The study demonstrates that GHRP-6 can increase the transcription levels of antimicrobial peptides in tilapia, enhancing their immune system activity against Pseudomonas aeruginosa infection.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Immunology
Ileanet Avalos, Thailin Lao, Elsa Maria Rodriguez, Yasser Zamora, Alianet Rodriguez, Ailyn Ramon, Gilda Lemos, Ania Cabrales, Monica Bequet-Romero, Dionne Casillas, Ivan Andujar, Luis Ariel Espinosa, Luis Javier Gonzalez, Yanitza Alvarez, Yamila Carpio, Mario Pablo Estrada
Summary: COVID-19 is a highly contagious respiratory viral disease caused by the novel coronavirus SARS-CoV-2. Researchers have developed a recombinant protein by fusing the receptor-binding domain (RBD) of the viral protein spike (S) with the extracellular domain of CD154, an immune system modulator molecule. This recombinant protein has shown promising immunogenicity in mice and non-human primates, inducing a specific and high IgG response and neutralizing the virus. It has the potential to enhance the current vaccines against COVID-19.
Article
Infectious Diseases
Janet Velazquez, Tania Rodriguez-Cornejo, Tania Rodriguez-Ramos, Geysi Perez-Rodriguez, Laura Rivera, James Hugh Campbell, Lowia Al-Hussinee, Yamila Carpio, Mario Pablo Estrada, Brian Dixon
Summary: PACAP has been found to have antimicrobial and immunomodulatory effects in fish, making it a potential alternative to antibiotics in the aquaculture industry. The study demonstrates that PACAP can directly inhibit bacterial growth and pre-treatment with low concentrations of the peptide significantly suppresses infection.
Article
Fisheries
Janet Velazquez, Lynn Cruz, Maylin Perez-Bernal, Onel Valdivia, Arlette Haidar, Alianet Rodriguez, Fidel Herrera, Osmany Gonzalez, Antonio Morales, Lisbet Ulloa, Reinaldo Blanco, Joel Perez, Dayami Dorta, Yaramis Luna, Hilda Elisa Garay, David Diago Abreu, Yassel Ramos, Vladimir Besada, Yeosvany Cabrera, Mario Pablo Estrada, Yamila Carpio
Summary: In this study, a monoclonal antibody (mAb) specific to Nile tilapia IgT was generated and confirmed for its specificity. The mAb showed immunoreactivity against recombinant IgT fragment and native IgT in skin mucus. The percentage of IgT+ B cell populations differed between juveniles and adults and after immunostimulation. This mAb serves as an important immunological tool for studying the biological function and structural characteristics of tilapia IgT.
FISH AND SHELLFISH IMMUNOLOGY REPORTS
(2023)
